Research Bio
Saliva is an essential fluid for maintaining not only oral homeostasis, but also for carrying out vital functions such as swallowing, chewing and lubrication. Some systemic diseases such as Sjögren syndrome, and traumatic or iatrogenic events like head and neck radiotherapy, can alter the salivary fluid rate, as well as the salivary composition.
Salivary gland secretion involves the dynamic interaction of the major and minor salivary glands and many other glandular structures which in a coordinated way excrete saliva into the oral cavity. The secretory process is a highly regulated mechanism in which Cl - channels, K + channels, Cl - /HCO 3 - exchangers, aquaporins, Na + /H + exchangers, carbonic anhydrases, Na + /K + /2Cl - co-transporter and the Na + /K + ATPase work in unison to generate saliva. The activation of these proteins on the basolateral and apical membranes of the acinar cells, determines the rate and direction of salt and water secretion, thus producing the primary saliva (isotonic) which is excreted into the lumen. Conversely, activation of the ion transport proteins expressed in ductal cells promotes the excretion of hypotonic saliva, due to the reabsorption of NaCl and the excretion of K + /HCO 3 - .
Within the last five years Proteomics has changed the understanding of molecular biology. Analytical methods and new techniques for proteomics have become available for characterizing thousands of proteins, gathering important information about their physicochemical properties, and studying their role in biological processes and human disease.
The main focus of my current research is to determine the molecular identity of some proteins involved in salivary secretion by MALDI-TOF Mass Spectroscopy in order to gather further understanding about their physiological role in health and disease processes.
| Clausula 90 FAAPA/UAEM Award" for Research Excellence (Health Sciences Area), School of Dentistry, National Autonomous University of the State of Mexico, Toluca, Mexico |
2001 |
| 4Th National Research Award"; Glaxo Wellcome, Mexico, City |
2000 |
| II Interstate Heath 2000 Research Award"; ISEM and ISSEMYM (Health Sciences Area), Toluca, Mexico |
2000 |
| Clausula 90 FAAPA/UAEM Award" for Research Excellence (Health Sciences Area), School of Dentistry, National Autonomous University of the State of Mexico, Toluca, Mexico |
1999 |
| National Dental Research Award" (1st place), National Dental Research Meeting, University of Veracruz; Xalapa, Veracruz |
1999 |
| Clausula 90 FAAPA/UAEM Award" for Research Excellence (Health Sciences Area), School of Dentistry, National Autonomous University of the State of Mexico, Toluca, Mexico |
1997 |
| Gustavo Baz Prada Award" (2nd place) for Social Service Excellence; School of Dentistry, National Autonomous University of Mexico; Mexico, City |
1995 |
| Edward H. Hatton Award" Boston MA, U.S.A |
1992 |
| Gabino Barreda Medal" for Academic Excellence and Outstanding Achievement in the School of Dentistry, National Autonomous University of Mexico; Mexico, City |
1990 |
| Honorable Mention" (Master. degree); School of Dentistry; National Autonomous University of Mexico; Mexico, City |
1989 |
| Honorable Mention" (D.D.S. degree); School of Dentistry; National Autonomous University of Mexico; Mexico, City |
1983 |
| Certificate of Academic Excellence" School of Dentistry; National Autonomous University of Mexico; Mexico, City |
1981 |
2010 Apr 23
Romanenko VG, Catalán MA, Brown DA, Putzier I, Hartzell HC, Marmorstein AD, Gonzalez-Begne M, Rock JR, Harfe BD, Melvin JE. "Tmem16A encodes the Ca2+-activated Cl- channel in mouse submandibular salivary gland acinar cells." The Journal of biological chemistry. 2010 Apr 23; 285(17):12990-3001. Epub 2010 Feb 22. |
2010 Feb 15
Catalán MA, Nakamoto T, Gonzalez-Begne M, Camden JM, Wall SM, Clarke LL, Melvin JE. "Cftr and ENaC ion channels mediate NaCl absorption in the mouse submandibular gland." The Journal of physiology. 2010 Feb 15; 588(Pt 4):713-24. Epub 2009 Dec 21. |
2009 Mar
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR. "Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT)." Journal of proteome research. 2009 Mar 0; 8(3):1304-14. |
2009 Feb 20
Nakamoto T, Brown DA, Catalán MA, Gonzalez-Begne M, Romanenko VG, Melvin JE. "Purinergic P2X7 receptors mediate ATP-induced saliva secretion by the mouse submandibular gland." The Journal of biological chemistry. 2009 Feb 20; 284(8):4815-22. Epub 2008 Dec 19. |
2009 Jan 1
Yan W, Apweiler R, Balgley BM, Boontheung P, Bundy JL, Cargile BJ, Cole S, Fang X, Gonzalez-Begne M, Griffin TJ, Hagen F, Hu S, Wolinsky LE, Lee CS, Malamud D, Melvin JE, Menon R, Mueller M, Qiao R, Rhodus NL, Sevinsky JR, States D, Stephenson JL, Than S, Yates JR, Yu W, Xie H, Xie Y, Omenn GS, Loo JA, Wong DT. "Systematic comparison of the human saliva and plasma proteomes." Proteomics. Clinical applications. 2009 Jan 1; 3(1):116-134. |
