Brian M. Ward, Ph.D.

Brian M. Ward, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 672
Rochester, NY 14642

Appointment: (585) 275-URMC (8762)

Patient Care Bio

Research Focus
Molecular Mechanisms of Poxvirus Envelope Formation
Research Overview
The Poxviridae family includes some of the largest DNA viruses known. While variola (the causative agent of smallpox) remains the most deadly member of the family, several other members, including monkeypox, tanapox, cowpox, vaccinia, Yaba-like disease virus and molluscum contagiosum, are capable of causing disease in humans. Orthopoxviruses, which include variola, monkeypox and vaccinia, have a double stranded genome of about 200 kb and are predicted to encode for approximately 200 functional open reading frames making them some the most complex animal viruses known.

This complexity is best demonstrated during viral morphogenesis that results in a virion that is predicted to incorporate approximately 100 viral polypeptides and several morphologically distinct forms. Viral replication occurs entirely in the cytoplasm in discrete areas know as viral factories and results in the first infectious form termed intracellular mature virions (IMV). A subset of IMV receives an extra double membrane wrapping derived from the trans-Golgi or endosomal cisternae and are referred to as intracellular enveloped virions (IEV). After wrapping, IEV are transported via microtubules to the cell periphery where the outer membrane of the IEV fuses with the plasma membrane depositing one of the newly acquired membranes into the plasma membrane and releasing the enveloped virion from the cell. Many enveloped virions remain attached to the plasma membrane and are termed cell-associated-virus (CEV). Viral proteins deposited into the plasma membrane, by the fusion of IEV at the plasma membrane, direct the polymerization of actin on the cytosolic side forming what are called actin tails, which serve to propel CEV away from the cell and towards adjacent cells. CEV released from the plasma membrane are termed extracellular enveloped virus (EEV). IEV, CEV and EEV make up the enveloped form of vaccinia virus and IMV are considered unenveloped. While IMV represents the majority of progeny virions they are not released from the cell making the enveloped form responsible for cell-to-cell spread. Presently only seven viral proteins have been found to be specific to the enveloped form, and of these seven only six have been shown to be required for efficient envelope virus production. The major focus of my laboratory is the study of poxvirus morphogenesis, emphasizing the intracellular envelopment process. We employ molecular virological techniques along with state of the art live video microscopy and cell biology to study viral egress with the goal of understanding the molecular mechanism employed by poxviruses to produce intracellular enveloped virions. Furthermore, our research should provide insight into such cellular processes as protein trafficking, membrane and vesicle formation and intracellular trafficking.

Awards & Honors (Local)

Who's Who in Medical Science Educatio | AcademicKeys 2005
Fellows Award For Research Excellence | NIH | Bethesda, MD 2003
Finalist - Norman P. Salzman Memorial Award In Virology | NIH | Bethesda MD 2002
Fellows Award For Research Excellence | NIH | Bethesda MD 2002
Finalist - Norman P. Salzman Memorial Award In Virology | NIH | Bethesda MD 2001
Fellows Award For Research Excellence | NIH | Bethesda MD 2001
Intramural Research Training Assistant Postdoctoral Fellowship | NIH | Bethesda MD 1998 - 2003
Francis M. and Harlie M. Clark Research Grant | University of Illinois | Urbana- Champaign, IL 1994
Howard Hughes Undergraduate Research Fellowship | Indiana University | Bloomington, IN 1991 - 1992
High Scholastic Achievement | Indiana University | Bloomington, IN 1991 - 1992

Current Appointments

Associate Professor - Department of Microbiology and Immunology (SMD) - Primary

Education

PhD | Microbiology | Univ of Illinois-Urbana1998
MS | Microbiology | Univ of Illinois-Urbana1994
BS | Microbiology | Indiana University1992

Post-Doctoral Training & Residency

Postdoctoral Fellow, National Institutes of Health, Bethesda, MD. Advisor: Bernard Moss 2003