Toru Takimoto, D.V.M., Ph.D.

Toru Takimoto, D.V.M., Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 672
Rochester, NY 14642

Office: (585) 273-2856
Lab: (585) 273-2894
Fax: (585) 473-9573

Research Bio

RESEARCH ACTIVITIES



Research project in progress
The major areas of research in my laboratory are as follows:
Molecular mechanism of paramyxovirus assembly: Efficient assembly and release of progeny virions from infected cells are major determinants of viral pathogenicity. Molecular mechanism of paramyxovirus assembly is not well understood. We are characterizing the trafficking of viral nucleocapsid to the site of assembly at the plasma membrane to understand the cellular machinery viruses utilize for assembly of progeny virions. URSMD Collaborators = Brian Ward, Minsoo Kim.

Assembly of Influenza A viruses: Influenza viruses replicate and form nucleocapsid core in nuclei of infected cells. The progeny nucleocapsid cores are then transported to the plasma membrane budding sites. This study analyzes cellular and viral protein interactions required for the nuclear export of nucleocapsid and budding of progeny virions. URSMD Collaborators = Brian Ward

Role of Influenza Virus Polymerase in Host Adaptation: We participate in the virology research team of the New York Influenza Center of Excellence (NYICE), which is part of the NIH Centers of Excellence for Influenza Research and Surveillance (CEIRS) network. Our focus is on the influenza virus RNA polymerase, and understanding how it contributes to host adaptation. We characterize the polymerase activities of avian and human strains, and identify residues and subunits of the polymerase complex important for the enhanced activity in mammalian hosts in vitro and in vivo. URSMD Collaborators = David Topham, John Treanor, Steve Dewhurst, Baek Kim.

Recent Journal Articles

Showing the 5 most recent journal articles. 54 available »

2014 Feb
Bialas KM, Bussey KA, Stone RL, Takimoto T. "Specific nucleoprotein residues affect influenza virus morphology." Journal of virology. 2014 Feb; 88(4):2227-34. Epub 2013 Dec 11.
2013 Mar
Desmet EA, Bussey KA, Stone R, Takimoto T. "Identification of the N-terminal domain of the influenza virus PA responsible for the suppression of host protein synthesis." Journal of virology. 2013 Mar; 87(6):3108-18. Epub 2013 Jan 02.
2013
Stone R, Takimoto T. "Critical role of the fusion protein cytoplasmic tail sequence in parainfluenza virus assembly." PloS one. 2013 8(4):e61281. Epub 2013 Apr 12.
2012 May
Noronha JM, Liu M, Squires RB, Pickett BE, Hale BG, Air GM, Galloway SE, Takimoto T, Schmolke M, Hunt V, Klem E, GarcĂ­a-Sastre A, McGee M, Scheuermann RH. "Influenza virus sequence feature variant type analysis: evidence of a role for NS1 in influenza virus host range restriction." Journal of virology. 2012 May; 86(10):5857-66. Epub 2012 Mar 07.
2012 Apr 25
MacDonald LA, Aggarwal S, Bussey KA, Desmet EA, Kim B, Takimoto T. "Molecular interactions and trafficking of influenza A virus polymerase proteins analyzed by specific monoclonal antibodies." Virology. 2012 Apr 25; 426(1):51-9. Epub 2012 Feb 08.

Current Appointments

Associate Professor - Department of Microbiology and Immunology (SMD) - Primary

Education

PhD | Microbiology | Japan-Hokkaido University - Sapporo1994
DVM | Veterinary Medicine | Japan-Hokkaido University - Sapporo1989
MS | Microbiology | Japan-Hokkaido University - Sapporo1988
BS | Veterinary Medicine | Japan-Hokkaido University - Sapporo1986

Post-Doctoral Training & Residency

Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 1995