David A. Dean, Ph.D.

David A. Dean, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 651
Rochester, NY 14642

Office: (585) 276-3933
Administrative: (585) 275-5948
Lab: (585) 275-3577
Office: (585) 756-7780

Books

2007
Zhou, R., J. E. Norton, and D. A. Dean. "Electroporation-mediated Gene Delivery to the Lungs.". Electroporation Protocols: Experimental and Clinical Medicine (in press).. Totowa, NJ: S. Li (Ed). Humana Press, 2007.
2005
Gokay, K. E., and D. A. Dean.. "Nuclear import of DNA. .". Nucleocytoplasmic Transport. Georgetown, TX.: T. Tzvi and V. Citovsky (Eds). Landes Bioscience, 2005. 187-205
2004
Dean, D. A.. "Improving gene delivery and expression of GFP by cyclic stretch.". Live Cell Imaging: A Laboratory Manual. Cold Spring Harbor, NY: D. L. Spector and R. D. Goldman (Eds). Cold Spring Harbor Laboratory Press, 2004. 51-66
2002
Dean, D. A.. "Nucleocytoplasmic trafficking". Pharmaceutical perspectives of nucleic acid-based therapeutics. London: R. I. Mahato (Ed). Harwood Academic Publishers, 2002. 229-260

Journal Articles

Showing all 70 journal articles available.

2013 Oct
Dean DA. "Cell-specific targeting strategies for electroporation-mediated gene delivery in cells and animals." The Journal of membrane biology. 2013 Oct; 246(10):737-44. Epub 2013 Mar 24.
2013 Jun
Badding MA, Dean DA. "Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules." Gene therapy. 2013 Jun; 20(6):616-24. Epub 2012 Sep 27.
2011 Jul
Lin X, Dean DA. "Gene therapy for ALI/ARDS." Critical care clinics. 2011 Jul; 27(3):705-18.
2011 Feb
Eldib M, Dean DA. "Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics." Biotechnology and bioengineering. 2011 Feb; 108(2):446-53.
2010 Sep
Staversky RJ, Vitiello PF, Yee M, Callahan LM, Dean DA, O'Reilly MA. "Epithelial ablation of Bcl-XL increases sensitivity to oxygen without disrupting lung development." American journal of respiratory cell and molecular biology.. 2010 Sep; 43(3):376-85. Epub 2009 Oct 30.
2010 Sep
Kaufman CD, Geiger RC, Dean DA. "Electroporation- and mechanical ventilation-mediated gene transfer to the lung." Gene therapy. 2010 Sep; 17(9):1098-104. Epub 2010 Apr 29.
2010
DeGiulio, J. V., and D. A. Dean. "Alveolar Epithelial Cell-Specific Plasmid Nuclear Import." Gene Therapy. 2010; 17: 541-549. Link
2010
Wang, Y.; K. Heilig, A. Minto, S. Chen, M. Xiang, D. Dean, C. Geiger, A. Chang, D. Pravtcheva, M. Schlimme, D. Deb, Y. Wang, R. Quigg, and C. Heilig. "Nephron-Deficient FVB Mice Develop Rapidly Progressive Renal Failure and Heavy Albuminuria Involving Excess Glomerular GLUT1 and VEGF. Lab Investigation 90:83-97". Lab Invest. 2010; 90: 83-97. Link
2009 Oct
Yee M, Chess PR, McGrath-Morrow SA, Wang Z, Gelein R, Zhou R, Dean DA, Notter RH, O'Reilly MA. "Neonatal oxygen adversely affects lung function in adult mice without altering surfactant composition or activity." American journal of physiology. Lung cellular and molecular physiology.. 2009 Oct; 297(4):L641-9. Epub 2009 Jul 17.
2009
Geiger, R.C.; C.D. Kaufman; A.P. Lam; G.R.S. Budinger and D.A. Dean. "Tubulin acetylation and histone deacetylase 6 (HDAC6) activity in the lung under cyclic load." Am J Resp Cell Mol Biol. 2009; 40: 76-82. Link
2009
Munkonge, F.M.; V. Amin; J.Smith, R. Hooley; S. Xenariou; C. Nixon; M.A. Ward; N. Leeds; K.-Y. Leung, E. Hillery, U. Griesenbach; D.M. Geddes, E. H. Postel; D.A. Dean; M. J. Dunn, and E.W.F.W Alton. "Identification and Functional Characterisation of Cytoplasmic Determinants of Plasmid DNA Nuclear Import. J Bio Chem 284:26978-26987". J Bio Chem. 2009; 284: 26978-26987. Link
2009
Xavier J.; S. Singh; D.A. Dean; N. M. Rao, and V. Gopal. "Designed multi-domain protein as a carrier of nucleic acids into cells." J Control Release. 2009; 133: 154-160. Link
2009
Miller, A.M., and D.A. Dean. "Identification of protein cofactors necessary for sequence-specific plasmid DNA nuclear import." Mol Therapy. 2009; 17: 1897-1903. Link
2009
Aistrup, G.L.; R. Villuendas; J. Ng; A. Gilchris; T.W. Lynch; D. Gordon; I. Cokic, S. Mottl; R. Zhou; D.A. Dean; J.A. Wasserstrom; J.J. Goldberger; A.H. Kadish, and R. Arora. "Targeted G-Protein Inhibition as a Novel Approach to Decrease Vagal Atrial Fibrillation by Selective Parasympathetic Attenuation." Cardiovasc Res. 2009; 83: 481-492. Link
2008 Jul
Young JL, Zimmer WE, Dean DA. "Smooth muscle-specific gene delivery in the vasculature based on restriction of DNA nuclear import." Experimental biology and medicine (Maywood, N.J.). 2008 Jul; 233(7):840-8. Epub 2008 Apr 29.
2008
Lam, A.P. and D.A. Dean. "Cyclic stretch-induced nuclear localization of transcription factors results in increased nuclear targeting of plasmids in alveolar epithelial cells." J Gene Medicine. 2008; 10: 668-678. Link
2008
Dean, D.A.; Ramanathan, T.; Machado, D.; and Sundarajan, R. "Electrical impedance spectroscopy study of biological tissues." J Electrostatistics. 2008; 66: 165-177. Link
2008
Capaccio, C.; Stoykov, N.S.; Sundarajan, R.; and D.A. Dean. "Quantifying Induced Electric Field Strengths During Gene Transfer to the Intact Rat Vasculature." J Electrostatics. 2008; : (in press). Link
2008
Miller, A.M. and D.A. Dean. "Cell Specific nuclear import of plasmid DNA in smooth muscle requires tissue-specific transcription factors and DNA sequences." Gene Ther. 2008; 15: 1107-1115. Link
2008
Vaughan, E.E., R.C. Geiger; A.M. Miller; T. Suzuki; N. Miyata; and D.A. Dean. "Microtubule acetylation via HDAC6 inhibition results increased transfection efficiency. Mol Therapy 16:1841-1847." Mol Thearpy. 2008; 16: 1841-1847. Link
2007 Oct
Mutlu GM, Green D, Bellmeyer A, Baker CM, Burgess Z, Rajamannan N, Christman JW, Foiles N, Kamp DW, Ghio AJ, Chandel NS, Dean DA, Sznajder JI, Budinger GR. "Ambient particulate matter accelerates coagulation via an IL-6-dependent pathway." The Journal of clinical investigation.. 2007 Oct; 117(10):2952-61.
2007 Sep 15
Mutlu GM, Machado-Aranda D, Norton JE, Bellmeyer A, Urich D, Zhou R, Dean DA. "Electroporation-mediated gene transfer of the Na+,K+ -ATPase rescues endotoxin-induced lung injury." American journal of respiratory and critical care medicine.. 2007 Sep 15; 176(6):582-90. Epub 2007 Jun 07.
2007 Mar 15
Bellmeyer A, Martino JM, Chandel NS, Scott Budinger GR, Dean DA, Mutlu GM. "Leptin resistance protects mice from hyperoxia-induced acute lung injury." American journal of respiratory and critical care medicine.. 2007 Mar 15; 175(6):587-94. Epub 2006 Dec 21.
2007 Mar
Zhou R, Dean DA. "Gene transfer of interleukin 10 to the murine cornea using electroporation." Experimental biology and medicine (Maywood, N.J.). 2007 Mar; 232(3):362-9.
2007 Feb
Kopp K, Gasiorowski JZ, Chen D, Gilmore R, Norton JT, Wang C, Leary DJ, Chan EK, Dean DA, Huang S. "Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells." Molecular biology of the cell. 2007 Feb; 18(2):394-403. Epub 2006 Nov 15.
2007
Capaccio, C., N. S. Stoykov, R. Sundararajan, and D. A. Dean. "Quantifying Induced Electric Field Strengths During Gene Transfer to the Intact Rat Vasculature." IEEE Trans. Biomed. Eng.. 2007; . Link
2007
Zhou, R.; J.E. Norton and D.A. Dean. "Electroporation-mediated Gene Delivery to the Lungs." Methods Molecular Biology. 2007; 423: 233-247. Link
2007
Gasiorowski JZ, Dean DA. "Intranuclear trafficking of episomal DNA is transcription-dependent." Molecular therapy : the journal of the American Society of Gene Therapy. 2007 15(12):2132-9. Epub 2007 Jul 31.
2006 May 15
Hillery E, Munkonge FM, Xenariou S, Dean DA, Alton EW. "Nondisruptive, sequence-specific coupling of fluorochromes to plasmid DNA." Analytical biochemistry.. 2006 May 15; 352(2):169-75. Epub 2006 Mar 09.
2006 Apr
Geiger RC, Taylor W, Glucksberg MR, Dean DA. "Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer." Gene therapy. 2006 Apr; 13(8):725-31.
2006 Feb
Vaughan EE, Dean DA. "Intracellular trafficking of plasmids during transfection is mediated by microtubules." Molecular therapy : the journal of the American Society of Gene Therapy. 2006 Feb; 13(2):422-8. Epub 2005 Nov 21.
2006
Zhou, R., J. E. Norton, N. Zhang, and D. A. Dean. "Electroporation-mediated transfer plasmids to the lung results in reduced TLR9 signaling and inflammation." Gene Therapy. 2006; . Link
2005 Sep
Gasiorowski JZ, Dean DA. "Postmitotic nuclear retention of episomal plasmids is altered by DNA labeling and detection methods." Molecular therapy : the journal of the American Society of Gene Therapy. 2005 Sep; 12(3):460-7.
2005 Jun 15
Jones JC, Lane K, Hopkinson SB, Lecuona E, Geiger RC, Dean DA, Correa-Meyer E, Gonzales M, Campbell K, Sznajder JI, Budinger S. "Laminin-6 assembles into multimolecular fibrillar complexes with perlecan and participates in mechanical-signal transduction via a dystroglycan-dependent, integrin-independent mechanism." Journal of cell science.. 2005 Jun 15; 118(Pt 12):2557-66. Epub 2005 May 31.
2005 Feb 1
Machado-Aranda D, Adir Y, Young JL, Briva A, Budinger GR, Yeldandi AV, Sznajder JI, Dean DA. "Gene transfer of the Na+,K+-ATPase beta1 subunit using electroporation increases lung liquid clearance." American journal of respiratory and critical care medicine.. 2005 Feb 1; 171(3):204-11. Epub 2004 Oct 29.
2005 Jan
Wang L, Dean DA, Macdonald RC. "Effect of vinblastine on transfection: influence of cell types, cationic lipids and promoters." Current drug delivery. 2005 Jan; 2(1):93-6.
2004 Apr
Zhang G, Gao X, Song YK, Vollmer R, Stolz DB, Gasiorowski JZ, Dean DA, Liu D. "Hydroporation as the mechanism of hydrodynamic delivery." Gene therapy. 2004 Apr; 11(8):675-82.
2003 Nov
Nunamaker EA, Zhang HY, Shirasawa Y, Benoit JN, Dean DA. "Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression." American journal of physiology. Heart and circulatory physiology. 2003 Nov; 285(5):H2240-7. Epub 2003 Jul 24.
2003 Sep
Dean DA, Machado-Aranda D, Blair-Parks K, Yeldandi AV, Young JL. "Electroporation as a method for high-level nonviral gene transfer to the lung." Gene therapy. 2003 Sep; 10(18):1608-15.
2003 Aug
Young JL, Benoit JN, Dean DA. "Effect of a DNA nuclear targeting sequence on gene transfer and expression of plasmids in the intact vasculature." Gene therapy. 2003 Aug; 10(17):1465-70.
2003 Jun 16
Munkonge FM, Dean DA, Hillery E, Griesenbach U, Alton EW. "Emerging significance of plasmid DNA nuclear import in gene therapy." Advanced drug delivery reviews. 2003 Jun 16; 55(6):749-60.
2003 May 1
Shirasawa Y, Rutland TJ, Young JL, Dean DA, Joseph BN. "Modulation of protein kinase C (PKC)-mediated contraction and the possible role of PKC epsilon in rat mesenteric arteries." Frontiers in bioscience : a journal and virtual library. 2003 May 1; 8:a133-8. Epub 2003 May 01.
2003 Apr
Taylor W, Gokay KE, Capaccio C, Davis E, Glucksberg M, Dean DA. "The effects of cyclic stretch on gene transfer in alveolar epithelial cells." Molecular therapy : the journal of the American Society of Gene Therapy. 2003 Apr; 7(4):542-9.
2002 May 3
Budinger GR, Tso M, McClintock DS, Dean DA, Sznajder JI, Chandel NS. "Hyperoxia-induced apoptosis does not require mitochondrial reactive oxygen species and is regulated by Bcl-2 proteins." The Journal of biological chemistry.. 2002 May 3; 277(18):15654-60. Epub 2002 Feb 27.
2002 Feb 22
Hirschi KK, Lai L, Belaguli NS, Dean DA, Schwartz RJ, Zimmer WE. "Transforming growth factor-beta induction of smooth muscle cell phenotpye requires transcriptional and post-transcriptional control of serum response factor." The Journal of biological chemistry.. 2002 Feb 22; 277(8):6287-95. Epub 2001 Dec 11.
2002 Jan
Young JL, Dean DA. "Nonviral gene transfer strategies for the vasculature." Microcirculation (New York, N.Y. : 1994). 2002 Jan; 9(1):35-49.
2002
Blair-Parks K, Weston BC, Dean DA. "High-level gene transfer to the cornea using electroporation." The journal of gene medicine. 2002 4(1):92-100.
2002
Filmore RA, Dean DA, Zimmer WE. "The smooth muscle gamma-actin gene is androgen responsive in prostate epithelia." Gene expression. 2002 10(5-6):201-11.
2001 Mar
Li S, MacLaughlin FC, Fewell JG, Gondo M, Wang J, Nicol F, Dean DA, Smith LC. "Muscle-specific enhancement of gene expression by incorporation of SV40 enhancer in the expression plasmid." Gene therapy. 2001 Mar; 8(6):494-7.
2001
Dean, D. A., G. Urban, I. V. Aragon, M. Swingle, S. Rusconi, M. Bueno, B. Miller, N. M. Dean, and R. E. Honkanen. "Serine / Threonine Protein Phosphatase 5 (PP5) Participates in the Regulation of Glucocorticoid Receptor Nucleocytoplasmic Shuttling." BioMed Central. 2001; 2: 6-14. Link
2000
Bustamante, J. O., E.R.F. Michelette, J.P. Geibel, D. A. Dean, J.A. Hanover, and T.J. McDonnell. "Calcium, ATP, and nuclear pore channel gating. Pflügers Archiv. Eur." J. Physiol.. 2000; 439: 433-444. Link
2000
Bustamante, J. O., E.R.F. Michelette, J.P. Geibel, J.A. Hanover, T.J. McDonnell, and D. A. Dean. "Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Archiv." Eur. J. Physiol.. 2000; 439: 838-844. Link
2000
Norwood, N., T. M. Moore, D. A. Dean, R. Bahattacharjee, M. Li, and T. Stevens. "Store-operated calcium entry and increased endothelial cell permeability." Am. J. Physiol. Lung Cell Mol. Physiol.. 2000; 279: 815-824. Link
2000
Martin JB, Young JL, Benoit JN, Dean DA. "Gene transfer to intact mesenteric arteries by electroporation." Journal of vascular research. 2000 37(5):372-80.
1999 Nov
Reddy JA, Dean D, Kennedy MD, Low PS. "Optimization of folate-conjugated liposomal vectors for folate receptor-mediated gene therapy." Journal of pharmaceutical sciences.. 1999 Nov; 88(11):1112-8.
1999 Jul 1
Wang G, Xu X, Pace B, Dean DA, Glazer PM, Chan P, Goodman SR, Shokolenko I. "Peptide nucleic acid (PNA) binding-mediated induction of human gamma-globin gene expression." Nucleic acids research.. 1999 Jul 1; 27(13):2806-13.
1999 Jun
Vacik J, Dean BS, Zimmer WE, Dean DA. "Cell-specific nuclear import of plasmid DNA." Gene therapy. 1999 Jun; 6(6):1006-14.
1999
Dean DA, Dean BS, Muller S, Smith LC. "Sequence requirements for plasmid nuclear import." Experimental cell research.. 1999 253(2):713-22.
1999
Dean, D. A., J. N. Byrd, Jr., and B. S. Dean. "Nuclear targeting of plasmid DNA in human corneal cells." Cur. Eye Res.. 1999; 19: 66-75. Link
1999
Wilson, G. L., G. Wang, B. S. Dean, and D. A. Dean. "Nuclear import of plasmid DNA in digitonin permeabilized cells requires both cytoplasmic extracts and specific DNA sequences." J. Biol. Chem.. 1999; 274: 22025–22032. Link
1997 Feb 1
Dean DA. "Import of plasmid DNA into the nucleus is sequence specific." Experimental cell research.. 1997 Feb 1; 230(2):293-302.
1997
Tran, M. T., D. A. Dean, R. N. Lausch, and J. E. Oakes. "Herpes Simplex Virus replicates in human corneal epithelial cells without enhancing IL-1??release." Virology. 1997; 244: 74-78. Link
1995 Feb
Dean DA, Li PP, Lee LM, Kasamatsu H. "Essential role of the Vp2 and Vp3 DNA-binding domain in simian virus 40 morphogenesis." Journal of virology.. 1995 Feb; 69(2):1115-21.
1994 Feb 18
Dean DA, Kasamatsu H. "Signal- and energy-dependent nuclear transport of SV40 Vp3 by isolated nuclei. Establishment of a filtration assay for nuclear protein import." The Journal of biological chemistry.. 1994 Feb 18; 269(7):4910-6.
1993
Clever, J.C., D.A. Dean, and H. Kasamatsu. "Identification of a DNA-binding domain within the simian virus 40 capsid proteins Vp2 and Vp3." J. Biol. Chem. 1993; 268: 29877-20883. Link
1992 Aug
Dean DA, Hor LI, Shuman HA, Nikaido H. "Interaction between maltose-binding protein and the membrane-associated maltose transporter complex in Escherichia coli." Molecular microbiology. 1992 Aug; 6(15):2033-40.
1990
Dean, D.A., J. Reizer, H. Nikaido, and M. H. Saier, Jr. "Regulation of the maltose transport system of Escherichia coli by the glucose-specific enzyme III of the PTS: characterization of inducer exclusion-resistant mutants and reconstitution of inducer exclusion in proteoliposomes." J. Biol. Chem.. 1990; 265: 15005-15010. Link
1990
Dean DA, Davidson AL, Nikaido H. "The role of ATP as the energy source for maltose transport in Escherichia coli." Research in microbiology. 1990 141(3):348-52.
1989 Jan
Dean DA, Fikes JD, Gehring K, Bassford PJ, Nikaido H. "Active transport of maltose in membrane vesicles obtained from Escherichia coli cells producing tethered maltose-binding protein." Journal of bacteriology.. 1989 Jan; 171(1):503-10.
1989
Dean DA, Davidson AL, Nikaido H. "Maltose transport in membrane vesicles of Escherichia coli is linked to ATP hydrolysis." Proceedings of the National Academy of Sciences of the United States of America.. 1989 86(23):9134-8.