Dorota Lucja Piekna-Przybylska, Ph.D.

Dorota Lucja Piekna-Przybylska, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 672
Rochester, NY 14642

Research Bio

HIV Replication and Latency: The Role of RNA and DNA G-quadruplexes.
G-quadruplexes are guanine rich nucleic acid structures capable of forming a four-stranded structure. DNA G-quadruplexes have been identified in telomeric DNA as well as more recently in mammalian chromosomal DNA - and are considered attractive targets in anti-cancer drug development studies.
We have recently shown that guanine-rich sequences in the HIV-1 genome can adopt G-quadruplex structures in both viral RNA and DNA. Viral RNA G-quadruplexes support genome dimerization and may facilitate viral recombination, which contributes to viral diversity.

We have also identified G-quadruplexes in the promoter region of HIV-1 proviral DNA (Sp1 binding sites). This suggests that these structures may also contribute to the regulation of viral gene expression (i.e., regulation of latency and reactivation). Our current research focuses on understanding the role of G-quadruplexes in HIV-1 DNA, and determining whether these structures may represent a new target for antiviral therapies.

Recent Journal Articles

Showing the 5 most recent journal articles. 12 available »

2014 Apr 29
Piekna-Przybylska D, Sullivan MA, Sharma G, Bambara RA. "U3 region in the HIV-1 genome adopts a G-quadruplex structure in its RNA and DNA sequence." Biochemistry.. 2014 Apr 29; 53(16):2581-93. Epub 2014 Apr 15.
2013 Aug 16
Piekna-Przybylska D, Sharma G, Bambara RA. "Mechanism of HIV-1 RNA dimerization in the central region of the genome and significance for viral evolution." The Journal of biological chemistry.. 2013 Aug 16; 288(33):24140-50. Epub 2013 Jul 09.
2011 Feb 20
Piekna-Przybylska D, Dykes C, Demeter LM, Bambara RA. "Sequences in the U3 region of human immunodeficiency virus 1 improve efficiency of minus strand transfer in infected cells." Virology.. 2011 Feb 20; 410(2):368-74. Epub 2010 Dec 30.
2011
Piekna-Przybylska D, Bambara RA. "Requirements for efficient minus strand strong-stop DNA transfer in human immunodeficiency virus 1." RNA biology. 2011 8(2):230-6. Epub 2011 Mar 01.
2010 Jan
Piekna-Przybylska D, Dichiacchio L, Mathews DH, Bambara RA. "A sequence similar to tRNA 3 Lys gene is embedded in HIV-1 U3-R and promotes minus-strand transfer." Nature structural & molecular biology.. 2010 Jan; 17(1):83-9. Epub 2009 Dec 06.

Current Appointments

Research Assistant Professor - Department of Microbiology and Immunology (SMD) - Primary

Education

PhD | Molecular Biology | Poland-Adam Mickiewicz Univ2002
MSc | Biochemistry | Poland-Adam Mickiewicz Univ1997

Post-Doctoral Training & Residency

Postdoctoral Research Associate - Laboratory of Dr. Robert Bambara. University of Rochester, Rochester, NY, United States 2011
Postdoctoral Research Associate - Laboratory of Dr. Maurille (Skip) Fournier. University of Massachusetts, Amherst, MA, United States 2008