Hinkle Lab

Patricia M. Hinkle, Ph.D.

Patricia M. Hinkle, Ph.D.
View Hinkle profile
Professor Emeritus

Affiliations:

 

 

 

 

Research Overview

We are interested in the broad question of how a hormone triggers a characteristic set of cellular responses. We use a number of model systems (pituitary cells, cell lines, transgenic animals) to study signal pathways for a hypothalamic peptide hormone, TRH, which acts via a G protein coupled receptor to increase cytoplasmic calcium and protein kinase C activity. Modern technologies allow us to study hormonal responses in live cells. For example, we can "tag" signaling proteins with green fluorescent protein (GFP) and then follow their movements in the cell during hormonal stimulation in real time. We monitor intracellular calcium in the cytoplasm and the endoplasmic reticulum using several fluorescence imaging techniques. In addition, we use biochemical and genetic approaches to identify proteins that interact with the receptor during biosynthesis, signaling and desensitization. Similar strategies are being used to ask how insulin secretion by the pancreatic beta cell is controlled. The long-term goal is to understand signal transduction in endocrine cells at a molecular and cellular level.

GFP-beta-arrestin

 

The movie shows the movement of GFP-beta-arrestin after TRH addition. When TRH binds to its receptor, the receptor changes conformation, activates Gq, and becomes phosphorylated by specific receptor kinases; the phospho-receptor binds to beta-arrestin. The movie covers about 10 min.

Recent Publications

Christian WV, Li N, Hinkle PM, and Ballatori N. (2012) β-Subunit of the Ostα-Ostβ organic solute transporter is required not only for heterodimerization and trafficking but also for function. J. Biol. Chem. 287:21233-21243.

Gehret AU, and Hinkle PM. (2012) siRNA Screen Identifies the Phosphatase Acting on the G Protein-Coupled Thyrotropin-Releasing Hormone Receptor. ACS Chem. Biol. [Epub ahead of print]

Hinkle PM, Gehret AU, and Jones BW. (2012) Desensitization, trafficking, and resensitization of the pituitary thyrotropin-releasing hormone receptor. Front. Neurosci. 6:180.

Thal DM, Homan KT, Chen J, Wu EK, Hinkle PM, Huang ZM, Chuprun JK, Song J, Gao E, Cheung JY, Sklar LA, Koch WJ, and Tesmer JJ. (2012) Paroxetine is a direct inhibitor of g protein-coupled receptor kinase 2 and increases myocardial contractility. ACS Chem. Biol. 7:1830-1839.

More papers

Contact Us

Patricia M. Hinkle, Ph.D.
University of Rochester
School of Medicine and Dentistry
Box 711
601 Elmwood Avenue
Rochester, NY 14642

Telephone: 585-275-4933
Fax: 585-273-2652

Patricia_Hinkle@
urmc.rochester.edu