Recent Publications
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Gershengorn, M.C., Heinflink, M., Nussenveig, D.R., Hinkle, P.M., and Falck-Pedersen, E. Thyrotropin-releasing hormone (TRH) receptor number determines the size of the TRH-responsive phosphoinositide pool. Demonstration using controlled expression of TRH receptors by adenovirus mediated gene transfer. J. Biol. Chem. 269: 6779-6783, 1994.

Nelson, E.J., Li, C.-R., Bangalore, R., Benson, T., Kass, R.S., and Hinkle, P.M. Inhibition of L-type calcium-channel activity by thapsigargin and 2,5-t-butylhydroquinone, but not by cyclopiazonic acid. Biochem. J. 302: 147-155, 1994.

Nelson, E.J. and Hinkle, P.M. Characteristics of the Ca²⁺ spike and oscillations induced by different doses of thyrotropin-releasing hormone (TRH) in individual pituitary cells and nonexcitable cells transfected with TRH receptor cDNA. Endocrinology 135: 1084-1092, 1994.

Nelson, E.J. and Hinkle, P.M. Thyrotropin-releasing hormone activates Ca²⁺ efflux: evidence suggesting that a plasma membrane Ca²⁺ pump is an effector for a G-protein coupled Ca²⁺-mobilizing receptor. J. Biol. Chem. 269: 30754-30860, 1994.

Ashworth, R., Yu, R., Nelson, E.J., Dermer, S., Gershengorn, M.C., and Hinkle, P.M. Visualization of the thyrotropin-releasing hormone receptor and its ligand during endocytosis and recycling. Proc. Natl. Acad. Sci. U.S.A. 92: 512-516, 1995.

Shupnik, M.A., Weck, J., and Hinkle, P.M. Thyrotropin (TSH)-releasing hormone stimulates TSH beta promoter activity by two distinct mechanisms involving calcium influx through L type Ca²⁺ channels and protein kinase C. Mol. Endocrinol. 10: 90-99, 1996.

Hinkle, P.M., Nelson, E.J., and Ashworth, R. Characterization of the calcium response to TRH in lactotrophs and GH-cells. Trends in Endocrinol. and Metab. 7: 370-374, 1996.

Ashworth, R. and Hinkle, P.M. Thyrotropin-releasing hormone-induced intracellular calcium responses in individual rat lactotrophs and thyrotrophs. Endocrinology 137: 5205-5212, 1996.

Kerper, L.E. and Hinkle, P.M. Cellular uptake of lead is activated by depletion of intracellular calcium stores. J. Biol. Chem. 272: 8346-8352, 1997.

Romoser, V.A., Hinkle, P.M., and Persechini, A. Detection in living cells of Ca²⁺-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators. J. Biol. Chem. 272: 13270-13274, 1997.

Yu, R. and Hinkle, P.M. Effect of cell type on the subcellular localization of the thyrotropin-releasing hormone receptor. Mol. Pharmacol. 51: 785-793, 1997.

Kerper, L.E. and Hinkle, P.M. Cellular uptake of lead is activated by depletion of intracellular calcium stores. J. Biol. Chem. 272: 8346-8352, 1997.

Yu, R. and Hinkle, P.M. Yu, R. and Hinkle, P.M. Desensitization of thyrotropin-releasing hormone receptor-mediated responses involves multiple steps. J. Biol. Chem. 272: 28301-28307, 1997.

Yu, R. and Hinkle, P.M. Signal transduction, desensitization and recovery of the thyrotropin-releasing hormone receptor after inhibition of receptor internalization. Mol. Endocrinol. 12: 737-749, 1998.

Yu, R., Ashworth, R., and Hinkle, P.M. Receptors for thyrotropin-releasing hormone on rat lactotropes and thyrotropes. Thyroid 8: 889-896, 1998.

Nelson, E.J., Zinkin, N.T. and Hinkle, P.M. Fluorescence methods to assess multidrug resistance in individual cells. Cancer Chemother. Pharmacol. 42: 292-299, 1998.

Fliss, M.S., Hinkle, P.M., and Bancroft, C. Expression cloning and characterization of PREB, a novel WD motif DNA-binding protein with a capacity to direct basal and protein kinase A-stimulated prolactin promoter activity. Mol. Endocrinol. 13: 644-657, 1999.

Yu, R. and Hinkle, P. M. Signal transduction and hormone-dependent internalization of the thyrotropin-releasing hormone receptor in cells lacking Gq and G11. J. Biol. Chem. 274: 15745-15750, 1999.

Xie, W., McLaughlin, J.P., Samoriski, G.M., Romoser, V.A., Smrcka, A., Hinkle, P.M., Bidlack, J.M., Gross, R.A., Jiang, H., and Wu, D. Genetic alteration of PLC beta3 expression modulates behavioral and cellular responses to mu opiods. Proc. Natl. Acad. Sci. U.S.A. 96: 10385-10390, 1999.

Yu, R., and Hinkle, P.M. Rapid turnover of calcium in the endoplasmic reticulum during signaling: studies with cameleon calcium indicators. J. Biol. Chem. 275: 23648-23653, 2000.

Smith, J., Yu, R., and Hinkle, P.M. Activation of MAPK by TRH requires clathrin-dependent endocytosis and PKC but not receptor interaction with beta-arrestin or receptor endocytosis. Mol Endocrinol. 15:1539-48, 2001.

Graves, T.K., Patel, S., Dannies, P.S., and Hinkle, P.M. Misfolded growth hormone causes Golgi fragmentation and disrupts ER-to-Golgi traffic. J. Cell Sci. 114:3685-94, 2001.

Zhu, C.-C., Boivin, L.M., and Hinkle, P.M. Dimerization and phosphorylation of thyrotropin-releasing hormone receptors are modulated by agonist stimulation. J. Biol. Chem. 277: 28228-37, 2002.

Hinkle, P.M., Pekary, A.E., Senanayaki, S., and Sattin, A. Role of TRH receptors as possible mediators of analeptic actions of TRH-like peptides. Brain Res. 935:59-64, 2002.

Hinkle, P.M. and Puskas, J.A. Detection of G Protein coupled receptors by immunofluorescence microscopy. In G Proteins and Their Receptors: Methods and Protocols (A. Smrcka, ed.), Methods in Molecular Biology, Humana Press, in press.

Graves, T.K. and Hinkle, P.M. Endoplasmic reticulum calcium storage and release in cells expressing misfolded growth hormone. IGF and Growth Hormone Res., in press, 2002.

Graves, T.K. and Hinkle, P.M. Ca²⁺-Induced Ca²⁺ Release in the pancreatic b-cell: Direct evidence of endoplasmic reticulum Ca²⁺ release. Submitted.

Boivin, L.M., Zhu, C.-C., and Hinkle, P.M. Ubiquitination of the thyrotropin-releasing hormone receptor. Submitted.

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