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David Dean

TitleProfessor
InstitutionSchool of Medicine and Dentistry
DepartmentPediatrics
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 651
Rochester NY 14642
Other Positions
TitleProfessor
InstitutionSchool of Medicine and Dentistry
DepartmentBiomedical Engineering

 
 Awards And Honors
1984     Presidents Undergraduate Scholarship, University of California, San Diego
1991 - 1994NIH Individual Postdoctoral Fellowship (NRSA)
2005     Sandler Program for Asthma Research, Unsolicited conversion of Junior Investigator Award status to Senior Investigator Award based on research productivity
2006     Mentoring the Mentors, Searle Center for Teaching Excellence, Northwestern University
 
 Overview
Despite all of the hype regarding gene therapy, at present, gene therapy is a dream due to insufficient levels of gene transfer and expression at desired sites. One way to increase gene expression is to target more DNA to the cell nucleus. Since the nucleus is the site of transcription, without the movement of plasmids through the cytoplasm, translocation into the nucleus, and localization to the appropriate subnuclear domain, no gene expression, or "gene therapy" can take place. Ongoing projects in my laboratory are studying the mechanisms of cytoplasmic trafficking of plasmids along the cytoskeleton, plasmid nuclear entry, subnuclear organization, and exploiting what we learn to improve gene therapy.


We have shown that plasmids are able to enter the nucleus in a sequence-specific manner that appears to be mediated by transcription factor binding. My lab is interested in identifying the proteins required for this activity and the regulation of their nuclear import. Based on our model, we have created cell-specific plasmids by incorporating DNA sequences that bind to cell-specific transcription factors. At present, we have examples of smooth muscle, alveolar epithelial cell, and endothelial cell-targeting vectors that we hope to use for the treatment of a number of pulmonary diseases, and we are working to expand our repertoire to selectively target expression to any desired cell or tissue.


We also are developing methods for extracellular delivery of non-viral vectors in animal models for disease. Using electroporation, we have obtained very high levels of gene expression in the vasculature and lungs of animals. We have used this approach to prevent and/or cure existing disease in models of acute lung injury and asthma in mice and rats. Our next goal is to optimize these approaches in more relevant preclinical models to move toward clinical application.

 
 Selected Publications
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  1. Young JL, Barravecchia MS, Dean DA. Electroporation-mediated gene delivery to the lungs. Methods Mol Biol. 2014; 1121:189-204.
    View in: PubMed
  2. Emr B, Gatto LA, Roy S, Satalin J, Ghosh A, Snyder K, Andrews P, Habashi N, Marx W, Ge L, Wang G, Dean DA, Vodovotz Y, Nieman G. Airway pressure release ventilation prevents ventilator-induced lung injury in normal lungs. JAMA Surg. 2013 Nov 1; 148(11):1005-12.
    View in: PubMed
  3. Dean DA. Cell-specific targeting strategies for electroporation-mediated gene delivery in cells and animals. J Membr Biol. 2013 Oct; 246(10):737-44.
    View in: PubMed
  4. Roy S, Habashi N, Sadowitz B, Andrews P, Ge L, Wang G, Roy P, Ghosh A, Kuhn M, Satalin J, Gatto LA, Lin X, Dean DA, Vodovotz Y, Nieman G. Early airway pressure release ventilation prevents ards-a novel preventive approach to lung injury. Shock. 2013 Jan; 39(1):28-38.
    View in: PubMed
  5. Badding MA, Lapek JD, Friedman AE, Dean DA. Proteomic and Functional Analyses of Protein-DNA Complexes During Gene Transfer. Mol Ther. 2013 Apr; 21(4):775-85.
    View in: PubMed
  6. Badding MA, Dean DA. Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules. Gene Ther. 2013 Jun; 20(6):616-24.
    View in: PubMed
  7. Cramer F, Christensen CL, Poulsen TT, Badding MA, Dean DA, Poulsen HS. Insertion of a nuclear factor kappa B DNA nuclear-targeting sequence potentiates suicide gene therapy efficacy in lung cancer cell lines. Cancer Gene Ther. 2012 Oct; 19(10):675-83.
    View in: PubMed
  8. Roy S, Sadowitz B, Andrews P, Gatto LA, Marx W, Ge L, Wang G, Lin X, Dean DA, Kuhn M, Ghosh A, Satalin J, Snyder K, Vodovotz Y, Nieman G, Habashi N. Early stabilizing alveolar ventilation prevents acute respiratory distress syndrome: a novel timing-based ventilatory intervention to avert lung injury. J Trauma Acute Care Surg. 2012 Aug; 73(2):391-400.
    View in: PubMed
  9. O'Reilly MA, Yee M, Buczynski BW, Vitiello PF, Keng PC, Welle SL, Finkelstein JN, Dean DA, Lawrence BP. Neonatal oxygen increases sensitivity to influenza A virus infection in adult mice by suppressing epithelial expression of Ear1. Am J Pathol. 2012 Aug; 181(2):441-51.
    View in: PubMed
  10. Yao H, Chung S, Hwang JW, Rajendrasozhan S, Sundar IK, Dean DA, McBurney MW, Guarente L, Gu W, Rönty M, Kinnula VL, Rahman I. SIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in mice. J Clin Invest. 2012 Jun 1; 122(6):2032-45.
    View in: PubMed
  11. Lin X, Dean DA. Gene therapy for ALI/ARDS. Crit Care Clin. 2011 Jul; 27(3):705-18.
    View in: PubMed
  12. Badding MA, Vaughan EE, Dean DA. Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer. Gene Ther. 2012 Mar; 19(3):338-46.
    View in: PubMed
  13. Dean DA, Gasiorowski JZ. Dendrimer-mediated transfection. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5584.
    View in: PubMed
  14. Dean DA, Gasiorowski JZ. Preparing injection pipettes on a flaming/brown pipette puller. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5586.
    View in: PubMed
  15. Dean DA, Gasiorowski JZ. Microinjecting cells using a pulsed-flow microinjection system. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5589.
    View in: PubMed
  16. Dean DA, Gasiorowski JZ. Nonviral gene delivery. Cold Spring Harb Protoc. 2011 Mar; 2011(3):top101.
    View in: PubMed
  17. Dean DA, Gasiorowski JZ. DNA sample preparation and loading sample into pipettes for microinjection of cells. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5588.
    View in: PubMed
  18. Dean DA, Gasiorowski JZ. Liposome-mediated transfection. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5583.
    View in: PubMed
  19. Dean DA, Gasiorowski JZ. Plating cells for microinjection. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5585.
    View in: PubMed
  20. Dean DA, Gasiorowski JZ. Preparing injection pipettes on a PUL-1 micropipette puller. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5587.
    View in: PubMed
  21. Dean DA, Gasiorowski JZ. Microinjecting cells using a constant-flow microinjection system. Cold Spring Harb Protoc. 2011 Mar; 2011(3):prot5590.
    View in: PubMed
  22. Eldib M, Dean DA. Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics. Biotechnol Bioeng. 2011 Feb; 108(2):446-53.
    View in: PubMed
  23. Kaufman CD, Geiger RC, Dean DA. Electroporation- and mechanical ventilation-mediated gene transfer to the lung. Gene Ther. 2010 Sep; 17(9):1098-104.
    View in: PubMed
  24. Lam AP, Dean DA. Progress and prospects: nuclear import of nonviral vectors. Gene Ther. 2010 Apr; 17(4):439-47.
    View in: PubMed
  25. Magovern JA, Moraca RJ, Bailey SH, Dean DA, Simpson KA, Maher TD, Benckart DH, Magovern GJ. Preoperative statin is associated with decreased operative mortality in high risk coronary artery bypass patients. J Cardiothorac Surg. 2010; 5:8.
    View in: PubMed
  26. Degiulio JV, Kaufman CD, Dean DA. The SP-C promoter facilitates alveolar type II epithelial cell-specific plasmid nuclear import and gene expression. Gene Ther. 2010 Apr; 17(4):541-9.
    View in: PubMed
  27. Wang Y, Heilig KO, Minto AW, Chen S, Xiang M, Dean DA, Geiger RC, Chang A, Pravtcheva DD, Schlimme M, Deb DK, Wang Y, Heilig CW. Nephron-deficient Fvb mice develop rapidly progressive renal failure and heavy albuminuria involving excess glomerular GLUT1 and VEGF. Lab Invest. 2010 Jan; 90(1):83-97.
    View in: PubMed
  28. Staversky RJ, Vitiello PF, Yee M, Callahan LM, Dean DA, O'Reilly MA. Epithelial ablation of Bcl-XL increases sensitivity to oxygen without disrupting lung development. Am J Respir Cell Mol Biol. 2010 Sep; 43(3):376-85.
    View in: PubMed
  29. Munkonge FM, Amin V, Hyde SC, Green AM, Pringle IA, Gill DR, Smith JW, Hooley RP, Xenariou S, Ward MA, Leeds N, Leung KY, Chan M, Hillery E, Geddes DM, Griesenbach U, Postel EH, Dean DA, Dunn MJ, Alton EW. Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear import. J Biol Chem. 2009 Sep 25; 284(39):26978-87.
    View in: PubMed
  30. Yee M, Chess PR, McGrath-Morrow SA, Wang Z, Gelein R, Zhou R, Dean DA, Notter RH, O'Reilly MA. Neonatal oxygen adversely affects lung function in adult mice without altering surfactant composition or activity. Am J Physiol Lung Cell Mol Physiol. 2009 Oct; 297(4):L641-9.
    View in: PubMed
  31. Miller AM, Munkonge FM, Alton EW, Dean DA. Identification of protein cofactors necessary for sequence-specific plasmid DNA nuclear import. Mol Ther. 2009 Nov; 17(11):1897-903.
    View in: PubMed
  32. Lim DS, Cortese CJ, Loree AN, Dean DA, Svitek RG. Left ventricular assist via percutaneous transhepatic transseptal cannulation in swine. Catheter Cardiovasc Interv. 2009 Jun 1; 73(7):961-5.
    View in: PubMed
  33. Aistrup GL, Villuendas R, Ng J, Gilchrist A, Lynch TW, Gordon D, Cokic I, Mottl S, Zhou R, Dean DA, Wasserstrom JA, Goldberger JJ, Kadish AH, Arora R. Targeted G-protein inhibition as a novel approach to decrease vagal atrial fibrillation by selective parasympathetic attenuation. Cardiovasc Res. 2009 Aug 1; 83(3):481-92.
    View in: PubMed
  34. Miller AM, Dean DA. Tissue-specific and transcription factor-mediated nuclear entry of DNA. Adv Drug Deliv Rev. 2009 Jul 2; 61(7-8):603-13.
    View in: PubMed
  35. Xavier J, Singh S, Dean DA, Rao NM, Gopal V. Designed multi-domain protein as a carrier of nucleic acids into cells. J Control Release. 2009 Jan 19; 133(2):154-60.
    View in: PubMed
  36. Vaughan EE, Geiger RC, Miller AM, Loh-Marley PL, Suzuki T, Miyata N, Dean DA. Microtubule acetylation through HDAC6 inhibition results in increased transfection efficiency. Mol Ther. 2008 Nov; 16(11):1841-7.
    View in: PubMed
  37. Geiger RC, Kaufman CD, Lam AP, Budinger GR, Dean DA. Tubulin acetylation and histone deacetylase 6 activity in the lung under cyclic load. Am J Respir Cell Mol Biol. 2009 Jan; 40(1):76-82.
    View in: PubMed
  38. Lam AP, Dean DA. Cyclic stretch-induced nuclear localization of transcription factors results in increased nuclear targeting of plasmids in alveolar epithelial cells. J Gene Med. 2008 Jun; 10(6):668-78.
    View in: PubMed
  39. Miller AM, Dean DA. Cell-specific nuclear import of plasmid DNA in smooth muscle requires tissue-specific transcription factors and DNA sequences. Gene Ther. 2008 Aug; 15(15):1107-15.
    View in: PubMed
  40. Trumble DR, Melvin DB, Dean DA, Magovern JA. In vivo performance of a muscle-powered drive system for implantable blood pumps. ASAIO J. 2008 May-Jun; 54(3):227-32.
    View in: PubMed
  41. Young JL, Zimmer WE, Dean DA. Smooth muscle-specific gene delivery in the vasculature based on restriction of DNA nuclear import. Exp Biol Med (Maywood). 2008 Jul; 233(7):840-8.
    View in: PubMed
  42. Zhou R, Norton JE, Dean DA. Electroporation-mediated gene delivery to the lungs. Methods Mol Biol. 2008; 423:233-47.
    View in: PubMed
  43. Mutlu GM, Green D, Bellmeyer A, Baker CM, Burgess Z, Rajamannan N, Christman JW, Foiles N, Kamp DW, Ghio AJ, Chandel NS, Dean DA, Sznajder JI, Budinger GR. Ambient particulate matter accelerates coagulation via an IL-6-dependent pathway. J Clin Invest. 2007 Oct; 117(10):2952-61.
    View in: PubMed
  44. Gasiorowski JZ, Dean DA. Intranuclear trafficking of episomal DNA is transcription-dependent. Mol Ther. 2007 Dec; 15(12):2132-9.
    View in: PubMed
  45. Sokos GG, Bolukoglu H, German J, Hentosz T, Magovern GJ, Maher TD, Dean DA, Bailey SH, Marrone G, Benckart DH, Elahi D, Shannon RP. Effect of glucagon-like peptide-1 (GLP-1) on glycemic control and left ventricular function in patients undergoing coronary artery bypass grafting. Am J Cardiol. 2007 Sep 1; 100(5):824-9.
    View in: PubMed
  46. Mutlu GM, Machado-Aranda D, Norton JE, Bellmeyer A, Urich D, Zhou R, Dean DA. Electroporation-mediated gene transfer of the Na+,K+ -ATPase rescues endotoxin-induced lung injury. Am J Respir Crit Care Med. 2007 Sep 15; 176(6):582-90.
    View in: PubMed
  47. Zhou R, Norton JE, Zhang N, Dean DA. Electroporation-mediated transfer of plasmids to the lung results in reduced TLR9 signaling and inflammation. Gene Ther. 2007 May; 14(9):775-80.
    View in: PubMed
  48. Zhou R, Dean DA. Gene transfer of interleukin 10 to the murine cornea using electroporation. Exp Biol Med (Maywood). 2007 Mar; 232(3):362-9.
    View in: PubMed
  49. Bellmeyer A, Martino JM, Chandel NS, Scott Budinger GR, Dean DA, Mutlu GM. Leptin resistance protects mice from hyperoxia-induced acute lung injury. Am J Respir Crit Care Med. 2007 Mar 15; 175(6):587-94.
    View in: PubMed
  50. Vaughan EE, DeGiulio JV, Dean DA. Intracellular trafficking of plasmids for gene therapy: mechanisms of cytoplasmic movement and nuclear import. Curr Gene Ther. 2006 Dec; 6(6):671-81.
    View in: PubMed
  51. Dean DA. Plating cells for microinjection. CSH Protoc. 2006; 2006(7).
    View in: PubMed
  52. Dean DA. Gene delivery by direct injection (microinjection) using a controlled-flow system. CSH Protoc. 2006; 2006(7).
    View in: PubMed
  53. Dean DA. Preparation (pulling) of needles for gene delivery by microinjection. CSH Protoc. 2006; 2006(7).
    View in: PubMed
  54. Dean DA. DNA preparation and needle loading for gene delivery by microinjection. CSH Protoc. 2006; 2006(7).
    View in: PubMed
  55. Dean DA. Gene delivery by direct injection (microinjection) using a pulsed-flow system. CSH Protoc. 2006; 2006(7).
    View in: PubMed
  56. Kopp K, Gasiorowski JZ, Chen D, Gilmore R, Norton JT, Wang C, Leary DJ, Chan EK, Dean DA, Huang S. Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells. Mol Biol Cell. 2007 Feb; 18(2):394-403.
    View in: PubMed
  57. Geiger RC, Taylor W, Glucksberg MR, Dean DA. Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer. Gene Ther. 2006 Apr; 13(8):725-31.
    View in: PubMed
  58. Hillery E, Munkonge FM, Xenariou S, Dean DA, Alton EW. Nondisruptive, sequence-specific coupling of fluorochromes to plasmid DNA. Anal Biochem. 2006 May 15; 352(2):169-75.
    View in: PubMed
  59. Vaughan EE, Dean DA. Intracellular trafficking of plasmids during transfection is mediated by microtubules. Mol Ther. 2006 Feb; 13(2):422-8.
    View in: PubMed
  60. Kuhn D, Dean D. Is developing scientific thinking all about learning to control variables? Psychol Sci. 2005 Nov; 16(11):866-70.
    View in: PubMed
  61. Gasiorowski JZ, Dean DA. Postmitotic nuclear retention of episomal plasmids is altered by DNA labeling and detection methods. Mol Ther. 2005 Sep; 12(3):460-7.
    View in: PubMed
  62. Dean DA. Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals. Am J Physiol Cell Physiol. 2005 Aug; 289(2):C233-45.
    View in: PubMed
  63. Dean DA, Strong DD, Zimmer WE. Nuclear entry of nonviral vectors. Gene Ther. 2005 Jun; 12(11):881-90.
    View in: PubMed
  64. Jones JC, Lane K, Hopkinson SB, Lecuona E, Geiger RC, Dean DA, Correa-Meyer E, Gonzales M, Campbell K, Sznajder JI, Budinger S. Laminin-6 assembles into multimolecular fibrillar complexes with perlecan and participates in mechanical-signal transduction via a dystroglycan-dependent, integrin-independent mechanism. J Cell Sci. 2005 Jun 15; 118(Pt 12):2557-66.
    View in: PubMed
  65. Wang L, Dean DA, Macdonald RC. Effect of vinblastine on transfection: influence of cell types, cationic lipids and promoters. Curr Drug Deliv. 2005 Jan; 2(1):93-6.
    View in: PubMed
  66. Zhou R, Geiger RC, Dean DA. Intracellular trafficking of nucleic acids. Expert Opin Drug Deliv. 2004 Nov; 1(1):127-40.
    View in: PubMed
  67. Machado-Aranda D, Adir Y, Young JL, Briva A, Budinger GR, Yeldandi AV, Sznajder JI, Dean DA. Gene transfer of the Na+,K+-ATPase beta1 subunit using electroporation increases lung liquid clearance. Am J Respir Crit Care Med. 2005 Feb 1; 171(3):204-11.
    View in: PubMed
  68. Zhang G, Gao X, Song YK, Vollmer R, Stolz DB, Gasiorowski JZ, Dean DA, Liu D. Hydroporation as the mechanism of hydrodynamic delivery. Gene Ther. 2004 Apr; 11(8):675-82.
    View in: PubMed
  69. Dean DA. Electroporation of the vasculature and the lung. DNA Cell Biol. 2003 Dec; 22(12):797-806.
    View in: PubMed
  70. Dean DA, Machado-Aranda D, Blair-Parks K, Yeldandi AV, Young JL. Electroporation as a method for high-level nonviral gene transfer to the lung. Gene Ther. 2003 Sep; 10(18):1608-15.
    View in: PubMed
  71. Young JL, Benoit JN, Dean DA. Effect of a DNA nuclear targeting sequence on gene transfer and expression of plasmids in the intact vasculature. Gene Ther. 2003 Aug; 10(17):1465-70.
    View in: PubMed
  72. Nunamaker EA, Zhang HY, Shirasawa Y, Benoit JN, Dean DA. Electroporation-mediated delivery of catalytic oligodeoxynucleotides for manipulation of vascular gene expression. Am J Physiol Heart Circ Physiol. 2003 Nov; 285(5):H2240-7.
    View in: PubMed
  73. Dean DA. Nuclear transport: an emerging opportunity for drug targeting. Adv Drug Deliv Rev. 2003 Jun 16; 55(6):699-702.
    View in: PubMed
  74. Gasiorowski JZ, Dean DA. Mechanisms of nuclear transport and interventions. Adv Drug Deliv Rev. 2003 Jun 16; 55(6):703-16.
    View in: PubMed
  75. Munkonge FM, Dean DA, Hillery E, Griesenbach U, Alton EW. Emerging significance of plasmid DNA nuclear import in gene therapy. Adv Drug Deliv Rev. 2003 Jun 16; 55(6):749-60.
    View in: PubMed
  76. Shirasawa Y, Rutland TJ, Young JL, Dean DA, Joseph BN. Modulation of protein kinase C (PKC)-mediated contraction and the possible role of PKC epsilon in rat mesenteric arteries. Front Biosci. 2003 May 1; 8:a133-8.
    View in: PubMed
  77. Taylor W, Gokay KE, Capaccio C, Davis E, Glucksberg M, Dean DA. The effects of cyclic stretch on gene transfer in alveolar epithelial cells. Mol Ther. 2003 Apr; 7(4):542-9.
    View in: PubMed
  78. Jia CX, Rabkin DG, Hart JP, Dean DA, Cabreriza SA, Weinberg AD, Spotnitz HM. Regional variation in myocardial water content in the edematous pig heart. J Surg Res. 2002 Jul; 106(1):70-5.
    View in: PubMed
  79. Budinger GR, Tso M, McClintock DS, Dean DA, Sznajder JI, Chandel NS. Hyperoxia-induced apoptosis does not require mitochondrial reactive oxygen species and is regulated by Bcl-2 proteins. J Biol Chem. 2002 May 3; 277(18):15654-60.
    View in: PubMed
  80. Filmore RA, Dean DA, Zimmer WE. The smooth muscle gamma-actin gene is androgen responsive in prostate epithelia. Gene Expr. 2002; 10(5-6):201-11.
    View in: PubMed
  81. Blair-Parks K, Weston BC, Dean DA. High-level gene transfer to the cornea using electroporation. J Gene Med. 2002 Jan-Feb; 4(1):92-100.
    View in: PubMed
  82. Young JL, Dean DA. Nonviral gene transfer strategies for the vasculature. Microcirculation. 2002 Jan; 9(1):35-49.
    View in: PubMed
  83. Hirschi KK, Lai L, Belaguli NS, Dean DA, Schwartz RJ, Zimmer WE. Transforming growth factor-beta induction of smooth muscle cell phenotpye requires transcriptional and post-transcriptional control of serum response factor. J Biol Chem. 2002 Feb 22; 277(8):6287-95.
    View in: PubMed
  84. Dean DA, Urban G, Aragon IV, Swingle M, Miller B, Rusconi S, Bueno M, Dean NM, Honkanen RE. Serine/threonine protein phosphatase 5 (PP5) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling. BMC Cell Biol. 2001; 2:6.
    View in: PubMed
  85. Dean DA, Perkin RA. Gene therapy: if at first you don't succeed... Am Fam Physician. 2001 May 1; 63(9):1707, 1710, 1715-6.
    View in: PubMed
  86. Li S, MacLaughlin FC, Fewell JG, Gondo M, Wang J, Nicol F, Dean DA, Smith LC. Muscle-specific enhancement of gene expression by incorporation of SV40 enhancer in the expression plasmid. Gene Ther. 2001 Mar; 8(6):494-7.
    View in: PubMed
  87. Dean DA. Peptide nucleic acids: versatile tools for gene therapy strategies. Adv Drug Deliv Rev. 2000 Nov 15; 44(2-3):81-95.
    View in: PubMed
  88. Norwood N, Moore TM, Dean DA, Bhattacharjee R, Li M, Stevens T. Store-operated calcium entry and increased endothelial cell permeability. Am J Physiol Lung Cell Mol Physiol. 2000 Nov; 279(5):L815-24.
    View in: PubMed
  89. Martin JB, Young JL, Benoit JN, Dean DA. Gene transfer to intact mesenteric arteries by electroporation. J Vasc Res. 2000 Sep-Oct; 37(5):372-80.
    View in: PubMed
  90. Bustamante JO, Michelette ER, Geibel JP, Hanover JA, McDonnell TJ, Dean DA. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflugers Arch. 2000 Apr; 439(6):829-37.
    View in: PubMed
  91. Bustamante JO, Michelette ER, Geibel JP, Dean DA, Hanover JA, McDonnell TJ. Calcium, ATP and nuclear pore channel gating. Pflugers Arch. 2000 Feb; 439(4):433-44.
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  92. Dean DA, Dean BS, Muller S, Smith LC. Sequence requirements for plasmid nuclear import. Exp Cell Res. 1999 Dec 15; 253(2):713-22.
    View in: PubMed
  93. Reddy JA, Dean D, Kennedy MD, Low PS. Optimization of folate-conjugated liposomal vectors for folate receptor-mediated gene therapy. J Pharm Sci. 1999 Nov; 88(11):1112-8.
    View in: PubMed
  94. Wilson GL, Dean BS, Wang G, Dean DA. Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences. J Biol Chem. 1999 Jul 30; 274(31):22025-32.
    View in: PubMed
  95. Dean DA, Byrd JN, Dean BS. Nuclear targeting of plasmid DNA in human corneal cells. Curr Eye Res. 1999 Jul; 19(1):66-75.
    View in: PubMed
  96. Wang G, Xu X, Pace B, Dean DA, Glazer PM, Chan P, Goodman SR, Shokolenko I. Peptide nucleic acid (PNA) binding-mediated induction of human gamma-globin gene expression. Nucleic Acids Res. 1999 Jul 1; 27(13):2806-13.
    View in: PubMed
  97. Vacik J, Dean BS, Zimmer WE, Dean DA. Cell-specific nuclear import of plasmid DNA. Gene Ther. 1999 Jun; 6(6):1006-14.
    View in: PubMed
  98. Hart JP, Cabreriza SE, Dean DA, Jia CX, Spotnitz HM. Conductance artifacts in a novel in vitro model of ventriculothoracic electrical coupling. ASAIO J. 1999 Jan-Feb; 45(1):64-8.
    View in: PubMed
  99. Dean DA, Amirhamzeh MM, Jia CX, Cabreriza SE, Rabkin DG, Sciacca R, Dickstein ML, Spotnitz HM. Reversal of iatrogenic myocardial edema and related abnormalities of diastolic properties in the pig left ventricle. J Thorac Cardiovasc Surg. 1998 May; 115(5):1209-14.
    View in: PubMed
  100. Tran MT, Dean DA, Lausch RN, Oakes JE. Membranes of herpes simplex virus type-1-infected human corneal epithelial cells are not permeabilized to macromolecules and therefore do not release IL-1alpha. Virology. 1998 Apr 25; 244(1):74-8.
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  101. Cabreriza SE, Dean DA, Amirhamzeh MM, Jia CX, Sciacca R, Soto P, Spotnitz HM. A method for detecting changes in left ventricular mass during variations in filling volume. J Am Soc Echocardiogr. 1998 Apr; 11(4):356-64.
    View in: PubMed
  102. Argenziano M, Dean DA, Moazami N, Goldstein DJ, Rose EA, Spotnitz HM, Burkhoff D, Oz MC, Dickstein ML. Inhaled nitric oxide is not a myocardial depressant in a porcine model of heart failure. J Thorac Cardiovasc Surg. 1998 Mar; 115(3):700-8.
    View in: PubMed
  103. Dean DA, Jia CX, Cabreriza SE, Soto PF, Rabkin DG, Sardo MJ, Chalik N, Spotnitz HM. Retrograde coronary perfusion: effects on iatrogenic edema and diastolic properties. Ann Thorac Surg. 1998 Feb; 65(2):449-53.
    View in: PubMed
  104. Goldstein DJ, Dean DA, Smerling A, Oz MC, Burkhoff D, Dickstein ML. Inhaled nitric oxide is not a negative inotropic agent in a porcine model of pulmonary hypertension. J Thorac Cardiovasc Surg. 1997 Sep; 114(3):461-6.
    View in: PubMed
  105. Cabreriza SE, Dean DA, Jia CX, Dickstein ML, Spotnitz HM. Electrical isolation of the heart. Stabilizing parallel conductance for left ventricular volume measurement. ASAIO J. 1997 Sep-Oct; 43(5):M509-14.
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  106. Dean DA. Import of plasmid DNA into the nucleus is sequence specific. Exp Cell Res. 1997 Feb 1; 230(2):293-302.
    View in: PubMed
  107. Amirhamzeh MM, Dean DA, Jia CX, Cabreriza SE, Yano OJ, Burkhoff D, Spotnitz HM. Validation of right and left ventricular conductance and echocardiography for cardiac function studies. Ann Thorac Surg. 1996 Oct; 62(4):1104-9.
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  108. Kisloukhine VV, Dean DA. Validation of a novel ultrasound dilution method to measure cardiac output during hemodialysis. ASAIO J. 1996 Sep-Oct; 42(5):M906-7.
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  109. Amirhamzeh MM, Dean DA, Jia CX, Cabreriza SE, Starr JP, Sardo MJ, Chalik N, Dickstein ML, Spotnitz HM. Iatrogenic myocardial edema: increased diastolic compliance and time course of resolution in vivo. Ann Thorac Surg. 1996 Sep; 62(3):737-43.
    View in: PubMed
  110. Dean DA, Jia CX, Cabreriza SE, D'Alessandro DA, Dickstein ML, Sardo MJ, Chalik N, Spotnitz HM. Validation study of a new transit time ultrasonic flow probe for continuous great vessel measurements. ASAIO J. 1996 Sep-Oct; 42(5):M671-6.
    View in: PubMed
  111. Dean DA, Cabreriza SE, Spotnitz HM. Geometry and temperature dependence of conductance ventriculography. ASAIO J. 1995 Jul-Sep; 41(3):M673-7.
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  112. Dean DA, Li PP, Lee LM, Kasamatsu H. Essential role of the Vp2 and Vp3 DNA-binding domain in simian virus 40 morphogenesis. J Virol. 1995 Feb; 69(2):1115-21.
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  113. Dean DA, Mangold BL, Lewis FA. Comparison of two strains of Schistosoma mansoni with respect to the sites and kinetics of immune elimination in irradiated cercaria-immunized mice. J Parasitol. 1995 Feb; 81(1):43-7.
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  114. Osman A, el Ridi R, Guirguis N, Dean DA. Identification of Schistosoma mansoni antigens recognized by T cells of C57BL/6 mice immunized with gamma-irradiated cercariae. J Parasitol. 1994 Jun; 80(3):421-31.
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  115. Dean DA, Kasamatsu H. Signal- and energy-dependent nuclear transport of SV40 Vp3 by isolated nuclei. Establishment of a filtration assay for nuclear protein import. J Biol Chem. 1994 Feb 18; 269(7):4910-6.
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  116. Clever J, Dean DA, Kasamatsu H. Identification of a DNA binding domain in simian virus 40 capsid proteins Vp2 and Vp3. J Biol Chem. 1993 Oct 5; 268(28):20877-83.
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  117. Dean DA, Hor LI, Shuman HA, Nikaido H. Interaction between maltose-binding protein and the membrane-associated maltose transporter complex in Escherichia coli. Mol Microbiol. 1992 Aug; 6(15):2033-40.
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  118. Kassim OO, Dean DA, Mangold BL, Von Lichtenberg F. Combined microautoradiographic and histopathologic analysis of the fate of challenge Schistosoma mansoni schistosomula in mice immunized with irradiated cercariae. Am J Trop Med Hyg. 1992 Aug; 47(2):231-7.
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  119. Dean DA, Mangold BL. Evidence that both normal and immune elimination of Schistosoma mansoni take place at the lung stage of migration prior to parasite death. Am J Trop Med Hyg. 1992 Aug; 47(2):238-48.
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  120. Perlak FJ, Fuchs RL, Dean DA, McPherson SL, Fischhoff DA. Modification of the coding sequence enhances plant expression of insect control protein genes. Proc Natl Acad Sci U S A. 1991 Apr 15; 88(8):3324-8.
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  121. Dean DA, Reizer J, Nikaido H, Saier MH. Regulation of the maltose transport system of Escherichia coli by the glucose-specific enzyme III of the phosphoenolpyruvate-sugar phosphotransferase system. Characterization of inducer exclusion-resistant mutants and reconstitution of inducer exclusion in proteoliposomes. J Biol Chem. 1990 Dec 5; 265(34):21005-10.
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  122. Dean DA, Davidson AL, Nikaido H. The role of ATP as the energy source for maltose transport in Escherichia coli. Res Microbiol. 1990 Mar-Apr; 141(3):348-52.
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  123. Dean DA, Davidson AL, Nikaido H. Maltose transport in membrane vesicles of Escherichia coli is linked to ATP hydrolysis. Proc Natl Acad Sci U S A. 1989 Dec; 86(23):9134-8.
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  124. Dean DA, Fikes JD, Gehring K, Bassford PJ, Nikaido H. Active transport of maltose in membrane vesicles obtained from Escherichia coli cells producing tethered maltose-binding protein. J Bacteriol. 1989 Jan; 171(1):503-10.
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  125. Georgi JR, Wade SE, Dean DA. Schistosoma mansoni: mechanism of attrition and routes of migration from lungs to hepatic portal system in the laboratory mouse. J Parasitol. 1987 Aug; 73(4):706-11.
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  126. Whittier RF, Dean DA, Rogers JC. Nucleotide sequence analysis of alpha-amylase and thiol protease genes that are hormonally regulated in barley aleurone cells. Nucleic Acids Res. 1987 Mar 25; 15(6):2515-35.
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  127. Mangold BL, Dean DA, Coulson PS, Wilson RA. Site requirements and kinetics of immune-dependent elimination of intravascularly administered lung stage schistosomula in mice immunized with highly irradiated cercariae of Schistosoma mansoni. Am J Trop Med Hyg. 1986 Mar; 35(2):332-44.
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  128. Dean DA, Mangold BL, Georgi JR, Jacobson RH. Comparison of Schistosoma mansoni migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis. Evidence that worm elimination occurs after the skin phase in immunized mice. Am J Trop Med Hyg. 1984 Jan; 33(1):89-96.
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  129. Mangold BL, Dean DA. Autoradiographic analysis of Schistosoma mansoni migration from skin to lungs in naive mice. Evidence that most attrition occurs after the skin phase. Am J Trop Med Hyg. 1983 Jul; 32(4):785-9.
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  130. Dean DA, Cioli D, Bukowski MA. Resistance induced by normal and irradiated Schistosoma mansoni: ability of various worm stages to serve as inducers and targets in mice. Am J Trop Med Hyg. 1981 Sep; 30(5):1026-32.
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  131. Damian RT, Greene ND, Suzuki T, Dean DA. Schistosomiasis mansoni in baboons. V. Antibodies and immediate hypersensitivity in multiply infected Papio cynocephalus. Am J Trop Med Hyg. 1981 Jul; 30(4):836-43.
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  132. Dean DA, Bukowski MA, Cheever AW. Relationship between acquired resistance, portal hypertension, and lung granulomas in ten strains of mice infected with Schistosoma mansoni. Am J Trop Med Hyg. 1981 Jul; 30(4):806-14.
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  133. Dean DA, Sell KW. Surface antigens on Schistosoma mansoni. II. Adsorption of a Forssman-like host antigen by schistosomula. Clin Exp Immunol. 1972 Dec; 12(4):525-40.
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