Marlise Klein
| Title | Research Assistant Professor |
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| Institution | School of Medicine and Dentistry |
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| Department | Dentistry in the Center for Oral Biology |
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| Address | University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 611
Rochester NY 14642
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| 2002 |
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| 2nd Place, Pediatric Dentistry Research Group Award, Brazilian Division of the International Association for Dental Research | | 2004 |
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| 1st Place, Junior Category, Edward S. Hatton Competition, American Division of the International Association for Dental Research | | 2008 |
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| 2nd Place, Postdoctoral Category, Edward S. Hatton Competition, American Association for Dental Research | | 2008 |
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| Unilever Travel Award, International Association for Dental Research | | 2009 |
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| William H. Bowen Award, Rochester Section of the American Association for Dental Research |
The major focus of my research has been on dental caries, which is increasing substantially in the world and putting more and more demand on resources of healthcare systems. Dental caries is a biofilm-dependent infectious disease that affects populations worldwide, and results from the interaction of specific bacteria and salivary constituents with dietary carbohydrates in the oral cavity. Streptococcus mutans are the major pathogens of dental caries. These organisms are able to succeed in the complex microbial community of the oral biofilm and cause caries because of their critical virulence properties: production of extracellular polysaccharides from sucrose, acidogenicity and acid tolerance.
The combination of sucrose and starch, the main dietary carbohydrates in modern societies, is potentially more cariogenic than either carbohydrate alone in humans. The biochemical reactions arising from interactions between specific salivary-amylases and bacterial-derived glucosyltransferases and dietary carbohydrates (sucrose and starch) promote cariogenic biofilm formation by Streptococcus mutans by increasing synthesis of the extracellular polysaccharide matrix and acidogenicity; which include modulation of expression of proven virulence genes (gtfBCD). Thus, understanding the roles of starch and sucrose in formation of biofilms of Streptococcus mutans and the microbial traits critical for enhancing the cariogenic potential of this bacterium could lead to the development of new therapeutic approach.
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Klein MI, Xiao J, Lu B, Delahunty CM, Yates JR, Koo H. Streptococcus mutans Protein Synthesis during Mixed-Species Biofilm Development by High-Throughput Quantitative Proteomics. PLoS One. 2012; 7(9):e45795.
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Falsetta ML, Klein MI, Lemos JA, Silva BB, Agidi S, Scott-Anne KK, Koo H. Novel Antibiofilm Chemotherapy Targets Exopolysaccharide Synthesis and Stress Tolerance in Streptococcus mutans To Modulate Virulence Expression In Vivo. Antimicrob Agents Chemother. 2012 Dec; 56(12):6201-11.
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Klein MI, Scott-Anne KM, Gregoire S, Rosalen PL, Koo H. Molecular approaches for viable bacterial population and transcriptional analyses in a rodent model of dental caries. Mol Oral Microbiol. 2012 Oct; 27(5):350-61.
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Xiao J, Klein MI, Falsetta ML, Lu B, Delahunty CM, Yates JR, Heydorn A, Koo H. The Exopolysaccharide Matrix Modulates the Interaction between 3D Architecture and Virulence of a Mixed-Species Oral Biofilm. PLoS Pathog. 2012 Apr; 8(4):e1002623.
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Gregoire S, Xiao J, Silva BB, Gonzalez I, Agidi PS, Klein MI, Ambatipudi KS, Rosalen PL, Bauserman R, Waugh RE, Koo H. Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces. Appl Environ Microbiol. 2011 Sep; 77(18):6357-67.
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Jeon JG, Pandit S, Xiao J, Gregoire S, Falsetta ML, Klein MI, Koo H. Influences of trans-trans farnesol, a membrane-targeting sesquiterpenoid, on Streptococcus mutans physiology and survival within mixed-species oral biofilms. Int J Oral Sci. 2011 Apr; 3(2):98-106.
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Catalán MA, Scott-Anne K, Klein MI, Koo H, Bowen WH, Melvin JE. Elevated incidence of dental caries in a mouse model of cystic fibrosis. PLoS One. 2011; 6(1):e16549.
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Klein MI, Xiao J, Heydorn A, Koo H. An analytical tool-box for comprehensive biochemical, structural and transcriptome evaluation of oral biofilms mediated by mutans streptococci. J Vis Exp. 2011; (47).
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Klein MI, DeBaz L, Agidi S, Lee H, Xie G, Lin AH, Hamaker BR, Lemos JA, Koo H. Dynamics of Streptococcus mutans transcriptome in response to starch and sucrose during biofilm development. PLoS One. 2010; 5(10):e13478.
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Koo H, Xiao J, Klein MI, Jeon JG. Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms. J Bacteriol. 2010 Jun; 192(12):3024-32.
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Koo H, Xiao J, Klein MI. Extracellular polysaccharides matrix--an often forgotten virulence factor in oral biofilm research. Int J Oral Sci. 2009 Dec; 1(4):229-34.
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Jeon JG, Klein MI, Xiao J, Gregoire S, Rosalen PL, Koo H. Influences of naturally occurring agents in combination with fluoride on gene expression and structural organization of Streptococcus mutans in biofilms. BMC Microbiol. 2009; 9:228.
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Klein MI, Duarte S, Xiao J, Mitra S, Foster TH, Koo H. Structural and molecular basis of the role of starch and sucrose in Streptococcus mutans biofilm development. Appl Environ Microbiol. 2009 Feb; 75(3):837-41.
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Alves de Souza R, Borges de Araújo Magnani MB, Nouer DF, Oliveira da Silva C, Klein MI, Sallum EA, Gonçalves RB. Periodontal and microbiologic evaluation of 2 methods of archwire ligation: ligature wires and elastomeric rings. Am J Orthod Dentofacial Orthop. 2008 Oct; 134(4):506-12.
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Aires CP, Del Bel Cury AA, Tenuta LM, Klein MI, Koo H, Duarte S, Cury JA. Effect of starch and sucrose on dental biofilm formation and on root dentine demineralization. Caries Res. 2008; 42(5):380-6.
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Barros LM, Boriollo MF, Alves AC, Klein MI, Gonçalves RB, Höfling JF. Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients. Arch Oral Biol. 2008 Dec; 53(12):1172-8.
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Duarte S, Klein MI, Aires CP, Cury JA, Bowen WH, Koo H. Influences of starch and sucrose on Streptococcus mutans biofilms. Oral Microbiol Immunol. 2008 Jun; 23(3):206-12.
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Klein MI, Bang S, Flório FM, Höfling JF, Gonçalves RB, Smith DJ, Mattos-Graner RO. Genetic diversity of competence gene loci in clinical genotypes of Streptococcus mutans. J Clin Microbiol. 2006 Aug; 44(8):3015-20.
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Alves AC, Napimoga MH, Klein MI, Hofling JF, Gonçalves RB. Increase in probing depth is correlated with a higher number of Prevotella intermedia genotypes. J Periodontol. 2006 Jan; 77(1):61-6.
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Napimoga MH, Höfling JF, Klein MI, Kamiya RU, Gonçalves RB. Tansmission, diversity and virulence factors of Sreptococcus mutans genotypes. J Oral Sci. 2005 Jun; 47(2):59-64.
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Machion L, Andia DC, Saito D, Klein MI, Gonçalves RB, Casati MZ, Nociti FH, Sallum EA. Microbiological changes with the use of locally delivered doxycycline in the periodontal treatment of smokers. J Periodontol. 2004 Dec; 75(12):1600-4.
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Klein MI, Flório FM, Pereira AC, Höfling JF, Gonçalves RB. Longitudinal study of transmission, diversity, and stability of Streptococcus mutans and Streptococcus sobrinus genotypes in Brazilian nursery children. J Clin Microbiol. 2004 Oct; 42(10):4620-6.
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Sallum EJ, Nouer DF, Klein MI, Gonçalves RB, Machion L, Wilson Sallum A, Sallum EA. Clinical and microbiologic changes after removal of orthodontic appliances. Am J Orthod Dentofacial Orthop. 2004 Sep; 126(3):363-6.
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Florio FM, Klein MI, Pereira AC, Goncalves BR. Time of initial acquisition of mutans streptococci by human infants. J Clin Pediatr Dent. 2004; 28(4):303-8.
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Klein MI, Gonçalves RB. Detection of Tannerella forsythensis (Bacteroides forsythus) and porphyromonas gingivalis by polymerase chain reaction in subjects with different periodontal status. J Periodontol. 2003 Jun; 74(6):798-802.
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