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Nancy Wang

TitleProfessor
InstitutionSchool of Medicine and Dentistry
DepartmentPathology and Laboratory Medicine
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 608
Rochester NY 14642
 
 Awards And Honors
1984     Appointed to mediate the Cancer and Developmental Genetics Session  | Annual meeting for the American Socieity of Human Genetics
1985 - 1986Charles E. Culpepper Fellowship
1986     Tenured Associate Professorship, Dept of Biochemistry
1989     America's Outstanding Women of the 80's-America's Who's Who
2000 - 2001President, Association of Chinese Geneticists in America
2009     Editorial Board  | Cancer, Genetics and Cytogenetics
 
 Overview
Microcell-mediated transfer of chromosome 11 into ovarian carcinoma cell line, SKOV-3 results in suppression of tumorigenicity in scid/scid mice providing evidence from functional aspect that chromosome 11 carries ovarian tumor suppressor gene(s).

To identify the differentially expressed transcripts associated with suppression of tumorigenicity, cDNA from the tumorigenic clones were subtracted from the non-tumorigenic clones which were then either cloned, sequenced and searched in GenBank, or analyzed by Gene Discovery Array Screening (GDAS). A cDNA transcript corresponding to the gene Thy-1 located (11q23-24) was found to be exclusively expressed in the non-tumorigenic clones. Transfection of Thy-1-antisence to the non-tumorigenic clone, 11(H)9-8, abolished the Thy-1 expression and restored tumorigenicity in scid/scid mice. We therefore, hypothesize that Thy-1 is a putative ovarian tumor suppressor gene (TSG).

To test this hypothesis, the following studies are ongoing: 1) To verify the tumor suppressing nature of Thy-1 and its specificity in fresh/frozen ovarian tumors by Northern blot, immunocytochemistry, and flow cytometry followed by statistical correlation study; 2) To define the effect of Thy-1 on in vivo growth and tumorigenicity by comparative xenographic study in scid/scid between 11(C)9-8-Thy-1-antisense and 11(C)9-8-null, as well as SKOV-3-Thy-1 and SKOV-3-null; 3) To identify the downstream genes associated with Thy-1 by comparative macroarray and microarray analysis combined with suppression subtractive hybridization on the DNA population between SKOV-3-Thy-1 and SKOV-3-null, as well as 11(C)9-8-Thy-1-antisense and 11(C)9-8-null; 4) To identify the sequential signaling pathway of Thy-1 using the in vitro doxycyclin (dox) Thy-1 inducible system established in our laboratory. cDNA obtained from SKOV-3-Thy-1-inducible cells at various time post dox exposure will be analyzed and compared between adjacent time points by both macroarray and dual color microarray.

The accomplishment of this study cannot only verify the OVC tumor suppressing nature of Thy-1, but also let us understand the function of this important cell surface marker.

 
 Selected Publications
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  1. Bunawan NC, Rastegar A, White KP, Wang NE. Djenkolism: case report and literature review. Int Med Case Rep J. 2014; 7:79-84.
    View in: PubMed
  2. Dean D, Wetzel B, White N, Kuppermann N, Wang NE, Haukoos JS, Hsia RY, Mann NC, Barton ED, Newgard CD. From 9-1-1 call to death: Evaluating traumatic deaths in seven regions for early recognition of high-risk patients. J Trauma Acute Care Surg. 2014 Mar; 76(3):846-53.
    View in: PubMed
  3. Wang NE, Saynina O, Vogel LD, Newgard CD, Bhattacharya J, Phibbs CS. The effect of trauma center care on pediatric injury mortality in California, 1999 to 2011. J Trauma Acute Care Surg. 2013 Oct; 75(4):704-16.
    View in: PubMed
  4. Yang DR, Lin SJ, Ding XF, Miyamoto H, Messing E, Li LQ, Wang N, Chang C. Higher Expression of Peroxisome Proliferator-activated Receptor ? or Its Activation by Agonist Thiazolidinedione-rosiglitazone Promotes Bladder Cancer Cell Migration and Invasion. Urology. 2013 May; 81(5):1109.e1-6.
    View in: PubMed
  5. Spence JM, Rothberg PG, Wang N, Burack WR. Demonstration of array-based analysis for highly multiplexed PCR assays application to detection of IGH@-BCL2 translocations in FFPE follicular lymphoma specimens. J Mol Diagn. 2011 May; 13(3):252-62.
    View in: PubMed
  6. Krishnamoorthi R, Ramarajan N, Wang NE, Newman B, Rubesova E, Mueller CM, Barth RA. Effectiveness of a staged US and CT protocol for the diagnosis of pediatric appendicitis: reducing radiation exposure in the age of ALARA. Radiology. 2011 Apr; 259(1):231-9.
    View in: PubMed
  7. Chen Y, Tsai Y, Nowak I, Wang N, Hyrien O, Wilkins R, Ferrarotto C, Sun H, Dertinger SD. Validating high-throughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: benchmark against dicentric and CBMN assays in a mouse model. Health Phys. 2010 Feb; 98(2):218-27.
    View in: PubMed
  8. Simon RA, Laughlin TS, Nuccie B, Wang N, Rothberg PG, Wang X. A 46 XY phenotypic female adolescent with bilateral gonadal tumors consisting of five different components. Int J Gynecol Pathol. 2008 Jul; 27(3):407-11.
    View in: PubMed
  9. Fender AB, Gust A, Wang N, Scott GA, Mercurio MG. Congenital leukemia cutis. Pediatr Dermatol. 2008 Jan-Feb; 25(1):34-7.
    View in: PubMed
  10. Szych CM, Liesveld JL, Iqbal MA, Li L, Siebert S, Asmus C, O'Malley J, Lee A, Wang N. Isodicentric Philadelphia chromosomes in imatinib mesylate (Gleevec)-resistant patients. Cancer Genet Cytogenet. 2007 Apr 15; 174(2):132-7.
    View in: PubMed
  11. Chintala SK, Wang N, Diskin S, Mattox C, Kagemann L, Fini ME, Schuman JS. Matrix metalloproteinase gelatinase B (MMP-9) is associated with leaking glaucoma filtering blebs. Exp Eye Res. 2005 Oct; 81(4):429-36.
    View in: PubMed
  12. Lung HL, Bangarusamy DK, Xie D, Cheung AK, Cheng Y, Kumaran MK, Miller L, Liu ET, Guan XY, Sham JS, Fang Y, Li L, Wang N, Protopopov AI, Zabarovsky ER, Tsao SW, Stanbridge EJ, Lung ML. THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma. Oncogene. 2005 Sep 29; 24(43):6525-32.
    View in: PubMed
  13. Abeysinghe HR, Li LQ, Guckert NL, Reeder J, Wang N. THY-1 induction is associated with up-regulation of fibronectin and thrombospondin-1 in human ovarian cancer. Cancer Genet Cytogenet. 2005 Sep; 161(2):151-8.
    View in: PubMed
  14. Patel D, Incassati A, Wang N, McCance DJ. Human papillomavirus type 16 E6 and E7 cause polyploidy in human keratinocytes and up-regulation of G2-M-phase proteins. Cancer Res. 2004 Feb 15; 64(4):1299-306.
    View in: PubMed
  15. Abeysinghe HR, Pollock SJ, Guckert NL, Veyberman Y, Keng P, Halterman M, Federoff HJ, Rosenblatt JP, Wang N. The role of the THY1 gene in human ovarian cancer suppression based on transfection studies. Cancer Genet Cytogenet. 2004 Feb; 149(1):1-10.
    View in: PubMed
  16. Abeysinghe HR, Cao Q, Xu J, Pollock S, Veyberman Y, Guckert NL, Keng P, Wang N. THY1 expression is associated with tumor suppression of human ovarian cancer. Cancer Genet Cytogenet. 2003 Jun; 143(2):125-32.
    View in: PubMed
  17. Komrokji R, Lancet J, Felgar R, Wang N, Bennett JM. Burkitt's leukemia with precursor B-cell immunophenotype and atypical morphology (atypical Burkitt's leukemia/lymphoma): case report and review of literature. Leuk Res. 2003 Jun; 27(6):561-6.
    View in: PubMed
  18. Segel GB, Woodlock TJ, Xu J, Li L, Felgar RE, Ryan DH, Lichtman MA, Wang N. Early gene activation in chronic leukemic B lymphocytes induced toward a plasma cell phenotype. Blood Cells Mol Dis. 2003 May-Jun; 30(3):277-87.
    View in: PubMed
  19. Wang N. Cytogenetics and molecular genetics of ovarian cancer. Am J Med Genet. 2002 Oct 30; 115(3):157-63.
    View in: PubMed
  20. Wang N. Methodologies in cancer cytogenetics and molecular cytogenetics. Am J Med Genet. 2002 Oct 30; 115(3):118-24.
    View in: PubMed
  21. Cao Q, Abeysinghe H, Chow O, Xu J, Kaung H, Fong C, Keng P, Insel RA, Lee WM, Barrett JC, Wang N. Suppression of tumorigenicity in human ovarian carcinoma cell line SKOV-3 by microcell-mediated transfer of chromosome 11. Cancer Genet Cytogenet. 2001 Sep; 129(2):131-7.
    View in: PubMed
  22. Abeysinghe HR, Cedrone E, Tyan T, Xu J, Wang N. Amplification of C-MYC as the origin of the homogeneous staining region in ovarian carcinoma detected by micro-FISH. Cancer Genet Cytogenet. 1999 Oct 15; 114(2):136-43.
    View in: PubMed
  23. Xu J, Fong CT, Cedrone E, Sullivan J, Wang N. Prenatal identification of de novo marker chromosomes using micro-FISH approach. Clin Genet. 1998 Jun; 53(6):490-6.
    View in: PubMed
  24. Choi C, Cho S, Horikawa I, Berchuck A, Wang N, Cedrone E, Jhung SW, Lee JB, Kerr J, Chenevix-Trench G, Kim S, Barrett JC, Koi M. Loss of heterozygosity at chromosome segment Xq25-26.1 in advanced human ovarian carcinomas. Genes Chromosomes Cancer. 1997 Nov; 20(3):234-42.
    View in: PubMed
  25. Xu J, Cedrone E, Roberts M, Wu G, Gershagen S, Wang N. The characterization of chromosomal rearrangements by a combined micro-FISH approach in a patient with myelodysplastic syndrome. Cancer Genet Cytogenet. 1995 Sep; 83(2):105-10.
    View in: PubMed
  26. Woodruff K, Wang N, May W, Adrone E, Denny C, Feig SA. The clonal nature of mediastinal germ cell tumors and acute myelogenous leukemia. A case report and review of the literature. Cancer Genet Cytogenet. 1995 Jan; 79(1):25-31.
    View in: PubMed
  27. Kouides PA, Phatak PD, Wang N, Bennett JM. B-cell acute lymphoblastic leukemia with L1 morphology and coexistence of t(1;19) and t(14;18) chromosome translocations. Cancer Genet Cytogenet. 1994 Nov; 78(1):23-7.
    View in: PubMed
  28. Thomas M, Kosciolek B, Wang N, Rowley P. Capping of bcr-abl antisense oligonucleotides enhances antiproliferative activity against chronic myeloid leukemia cell lines. Leuk Res. 1994 Jun; 18(6):401-8.
    View in: PubMed
  29. Xu J, Wang N. Identification of chromosomal structural alterations in human ovarian carcinoma cells using combined GTG-banding and repetitive fluorescence in situ hybridization (FISH). Cancer Genet Cytogenet. 1994 May; 74(1):1-7.
    View in: PubMed
  30. Sherer DM, Wang N, Thompson HO, Peterson JC, Miller ME, Metlay LA, Abramowicz JS. An infant with trisomy 9 mosaicism presenting as a complete trisomy 9 by amniocentesis. Prenat Diagn. 1992 Jan; 12(1):31-7.
    View in: PubMed
  31. Wang N, Youngblom J, Janatipour M, Shoffner RN. Differential evolution in chromosomal composition during multiple cycles of subcutaneous and intravenous metastasis. Cancer Genet Cytogenet. 1991 Jul 15; 54(2):205-14.
    View in: PubMed
  32. Wang N, Cedrone E, Dry J, Skuse GR, Schwartz C, Terryberry S. Transposition of the oncogene ets-1 in t(11;19) translocation in acute leukemia. Cancer Genet Cytogenet. 1990 Dec; 50(2):199-205.
    View in: PubMed
  33. Wang N, Cedrone E, Skuse GR, Insel R, Dry J. Two identical active X chromosomes in human mammary carcinoma cells. Cancer Genet Cytogenet. 1990 Jun; 46(2):271-80.
    View in: PubMed
  34. Youngblom JH, Wang N, McCarthy JB, Janatipour M, Wang T, Sheppard JR, Panter SS. Expression of a specific protein in spontaneously metastatic fibrosarcoma cell lines and its enhanced synthesis by growth on laminin or fibronectin. Clin Exp Metastasis. 1990 Jan-Feb; 8(1):47-58.
    View in: PubMed
  35. Pedersen MI, Wang N. Chromosomal evolution in the progression and metastasis of human malignant melanoma. A multiple lesion study. Cancer Genet Cytogenet. 1989 Sep; 41(2):185-201.
    View in: PubMed
  36. Blennerhassett GT, Furth ME, Anderson A, Burns JP, Chaganti RS, Blick M, Talpaz M, Dev VG, Chan LC, Wiedemann LM, et al. Clinical evaluation of a DNA probe assay for the Philadelphia (Ph1) translocation in chronic myelogenous leukemia. Leukemia. 1988 Oct; 2(10):648-57.
    View in: PubMed
  37. Wang N, Leung J, Warrier RP, Schorin M, Kirkpatrick D, Nowak MJ, Strand R. Nonrandom chromosomal aberrations and clonal chromosomal evolution in acute leukemia associated with Down's syndrome. Cancer Genet Cytogenet. 1987 Sep; 28(1):155-62.
    View in: PubMed
  38. Bear A, Clayman RV, Elbers J, Limas C, Wang N, Stone K, Gebhard R, Prigge W, Palmer J. Characterization of two human cell lines (TK-10, TK-164) of renal cell cancer. Cancer Res. 1987 Jul 15; 47(14):3856-62.
    View in: PubMed
  39. Varela M, Wang N, Cerrillo M. Prenatal diagnosis of a 46,XX,inv(12)pat/47,XX,i(Xq),inv(12)pat. Hum Genet. 1987 Jan; 75(1):93-4.
    View in: PubMed
  40. Rangan SR, Martin LN, Bozelka BE, Wang N, Gormus BJ. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int J Cancer. 1986 Sep 15; 38(3):425-32.
    View in: PubMed
  41. Hornung MO, Krementz ET, Sullivan KA, Muchmore JH, Prats I, Wang N, Strand R. Immunological heterogeneity in human melanoma: immunogenic alloantigen expression in autologous host. Cancer Res. 1986 Jul; 46(7):3704-10.
    View in: PubMed
  42. Gregory P, Wang N. Alterations in the X chromosome replication pattern induced by 5-azacytidine in a human tumor line. Cancer Genet Cytogenet. 1986 Feb 15; 20(3-4):263-7.
    View in: PubMed
  43. Pedersen MI, Bennett JW, Wang N. Nonrandom chromosome structural aberrations and oncogene loci in human malignant melanoma. Cancer Genet Cytogenet. 1986 Feb 1; 20(1-2):11-27.
    View in: PubMed
  44. Gilman PA, Wang N, Fan SF, Reede J, Khan A, Leventhal BG. Familial osteosarcoma associated with 13;14 chromosomal rearrangement. Cancer Genet Cytogenet. 1985 Jun; 17(2):123-32.
    View in: PubMed
  45. Andes WA, deShazo RD, Reed RJ, Harkin JC, Wang NN. Studies of lymph nodes from patients with classical hemophilia. Blood. 1984 Oct; 64(4):768-73.
    View in: PubMed
  46. Wang N, Perkins KL. Involvement of band 3p14 in t(3;8) hereditary renal carcinoma. Cancer Genet Cytogenet. 1984 Apr; 11(4):479-81.
    View in: PubMed
  47. Wang N, Trend B, Kaung HL, Wang T. Chromosomal replication asynchrony of a human breast carcinoma cell line. I. studied by continuous BrdU incorporation and G-banding analysis. Cancer Genet Cytogenet. 1982 Oct; 7(2):173-80.
    View in: PubMed
  48. Wang N, Shoffner RN, Otis JS, Cheng KM. The induction of chromosomal structural changes in male chickens by the alkylating agents: triethylene melamine and ethyl methanesulfonate. Mutat Res. 1982 Sep; 96(1):53-66.
    View in: PubMed
  49. Johnson MP, Ramsay N, Cervenka J, Wang N. Retinoblastoma and its association with a deletion in chromosome #13: a survey using high-resolution chromosome techniques. Cancer Genet Cytogenet. 1982 May; 6(1):29-37.
    View in: PubMed
  50. Kiang DT, King M, Zhang HJ, Kennedy BJ, Wang N. Cyclic biological expression in mouse mammary tumors. Science. 1982 Apr 2; 216(4541):68-70.
    View in: PubMed
  51. Flynn KJ, Dehner LP, Gajl-Peczalska KJ, Dahl MV, Ramsay N, Wang N. Regressing atypical histiocytosis: a cutaneous proliferation of atypical neoplastic histiocytes with unexpectedly indolent biologic behavior. Cancer. 1982 Mar 1; 49(5):959-70.
    View in: PubMed
  52. Wang N, Yu SH, Liener IE, Hebbel RP, Eaton JW, McKhann CF. Characterization of high- and low-metastatic clones derived from a methylcholanthrene-induced murine fibrosarcoma. Cancer Res. 1982 Mar; 42(3):1046-51.
    View in: PubMed
  53. Flynn KJ, Dehner LP, Gajl-Peczalska KJ, Dahl MV, Ramsay N, Wang N. Regressing atypical histiocytosis: benign or malignant? Minn Med. 1982 Feb; 65(2):73-6.
    View in: PubMed
  54. Bitgood JJ, Shoffner RN, Otis JS, Wang N. Recombinant inversion chromosomes in phenotypically normal chickens. Science. 1982 Jan 22; 215(4531):409-11.
    View in: PubMed
  55. Wiencke JK, Vosika J, Johnson P, Wang N, Garry VF. Differential induction of sister chromatid exchange by chemical carcinogens in lymphocytes cultured from patients with solid tumors. Pharmacology. 1982; 24(2):67-73.
    View in: PubMed
  56. Wang N, Perkins KL, Bronson DL, Fraley EE. Cytogenetic evidence for premeiotic transformation of human testicular cancers. Cancer Res. 1981 Jun; 41(6):2135-40.
    View in: PubMed
  57. Wang N, Sheppard JR, Wang T, Shoffner RN. The induction and detection of diploid spermatozoa in Gallus domesticus. Mutat Res. 1980 Dec; 73(2):279-90.
    View in: PubMed
  58. Wang N, Trend B, Bronson DL, Fraley EE. Nonrandom abnormalities in chromosome 1 in human testicular cancers. Cancer Res. 1980 Mar; 40(3):796-802.
    View in: PubMed
  59. Wang N, Shoffner RN. Trypsin G- and C-banding for interchange analysis and sex identification in the chicken. Chromosoma. 1974; 47(1):61-9.
    View in: PubMed

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