Mark Dumont
| Title | Professor |
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| Institution | School of Medicine and Dentistry |
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| Department | Biochemistry and Biophysics |
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| Address | University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 712
Rochester NY 14642
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Transmembrane proteins carry out critical functions in transporting ions and metabolites in an out of cells and organelles, in transducing extracellular signals into intracellular responses, in cell-cell recognition, and in catalyzing a variety of enzymatic reactions. They also constitute 20-30% of the protein coding potential of most genomes. However, the structures and molecular mechanisms of the function of membrane proteins remain much more poorly characterized than is the case for most soluble proteins. The Dumont laboratory focuses on understanding mechanisms of membrane protein function at the molecular level.
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Mathew E, Ding FX, Naider F, Dumont ME. Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast. Protein Eng Des Sel. 2013 Jan; 26(1):59-71.
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Gehret AU, Connelly SM, Dumont ME. Functional and Physical Interactions among Saccharomyces cerevisiae a-Factor Receptors. Eukaryot Cell. 2012 Oct; 11(10):1276-88.
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Lesoine JF, Venkataraman PA, Maloney PC, Dumont ME, Novotny L. Nanochannel-based single molecule recycling. Nano Lett. 2012 Jun 13; 12(6):3273-8.
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Taslimi A, Mathew E, Celic A, Wessel S, Dumont ME. Identifying Functionally Important Conformational Changes in Proteins: Activation of the Yeast a-factor Receptor Ste2p. J Mol Biol. 2012 May 18; 418(5):367-78.
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Kang DC, Venkataraman PA, Dumont ME, Maloney PC. Oligomeric state of the oxalate transporter, OxlT. Biochemistry. 2011 Oct 4; 50(39):8445-53.
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Mathew E, Bajaj A, Connelly SM, Sargsyan H, Ding FX, Hajduczok AG, Naider F, Dumont ME. Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p. J Mol Biol. 2011 Jun 17; 409(4):513-28.
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Tantry S, Ding FX, Dumont M, Becker JM, Naider F. Binding of fluorinated phenylalanine alpha-factor analogues to Ste2p: evidence for a cation-pi binding interaction between a peptide ligand and its cognate G protein-coupled receptor. Biochemistry. 2010 Jun 22; 49(24):5007-15.
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Shi S, Notenboom S, Dumont ME, Ballatori N. Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast. Cell Physiol Biochem. 2010; 25(2-3):293-306.
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Clark KM, Fedoriw N, Robinson K, Connelly SM, Randles J, Malkowski MG, DeTitta GT, Dumont ME. Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography. Protein Expr Purif. 2010 Jun; 71(2):207-23.
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Fan E, Baker D, Fields S, Gelb MH, Buckner FS, Van Voorhis WC, Phizicky E, Dumont M, Mehlin C, Grayhack E, Sullivan M, Verlinde C, Detitta G, Meldrum DR, Merritt EA, Earnest T, Soltis M, Zucker F, Myler PJ, Schoenfeld L, Kim D, Worthey L, Lacount D, Vignali M, Li J, Mondal S, Massey A, Carroll B, Gulde S, Luft J, Desoto L, Holl M, Caruthers J, Bosch J, Robien M, Arakaki T, Holmes M, Le Trong I, Hol WG. Structural genomics of pathogenic protozoa: an overview. Methods Mol Biol. 2008; 426:497-513.
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Chloupková M, Pickert A, Lee JY, Souza S, Trinh YT, Connelly SM, Dumont ME, Dean M, Urbatsch IL. Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity. Biochemistry. 2007 Jul 10; 46(27):7992-8003.
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Bajaj A, Connelly SM, Gehret AU, Naider F, Dumont ME. Role of extracellular charged amino acids in the yeast alpha-factor receptor. Biochim Biophys Acta. 2007 Jun; 1773(6):707-17.
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White MA, Clark KM, Grayhack EJ, Dumont ME. Characteristics affecting expression and solubilization of yeast membrane proteins. J Mol Biol. 2007 Jan 19; 365(3):621-36.
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Wang Z, Stalcup LD, Harvey BJ, Weber J, Chloupkova M, Dumont ME, Dean M, Urbatsch IL. Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8. Biochemistry. 2006 Aug 15; 45(32):9929-39.
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Gehret AU, Bajaj A, Naider F, Dumont ME. Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors. J Biol Chem. 2006 Jul 28; 281(30):20698-714.
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Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky EM, Snyder M, Grayhack EJ. Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes Dev. 2005 Dec 1; 19(23):2816-26.
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Bajaj A, Celic A, Ding FX, Naider F, Becker JM, Dumont ME. A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast. Biochemistry. 2004 Oct 26; 43(42):13564-78.
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Alexandrov A, Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, Babulski J, Mitchell SF, Schoenfeld LW, Fields S, Hol WG, Dumont ME, Phizicky EM, Grayhack EJ. A facile method for high-throughput co-expression of protein pairs. Mol Cell Proteomics. 2004 Sep; 3(9):934-8.
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Roginskaya M, Connelly SM, Kim KS, Patel D, Dumont ME. Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors. Mol Genet Genomics. 2004 Mar; 271(2):237-48.
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Celic A, Connelly SM, Martin NP, Dumont ME. Intensive mutational analysis of G protein-coupled receptors in yeast. Methods Mol Biol. 2004; 237:105-20.
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Celic A, Martin NP, Son CD, Becker JM, Naider F, Dumont ME. Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation. Biochemistry. 2003 Mar 18; 42(10):3004-17.
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Arevalo E, Estephan R, Madeo J, Arshava B, Dumont M, Becker JM, Naider F. Biosynthesis and biophysical analysis of domains of a yeast G protein-coupled receptor. Biopolymers. 2003; 71(4):516-31.
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Martin NP, Celic A, Dumont ME. Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor. J Mol Biol. 2002 Apr 12; 317(5):765-88.
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Erlenbach I, Kostenis E, Schmidt C, Serradeil-Le Gal C, Raufaste D, Dumont ME, Pausch MH, Wess J. Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis. J Biol Chem. 2001 Aug 3; 276(31):29382-92.
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Sommers CM, Martin NP, Akal-Strader A, Becker JM, Naider F, Dumont ME. A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor. Biochemistry. 2000 Jun 13; 39(23):6898-909.
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Leavitt LM, Macaluso CR, Kim KS, Martin NP, Dumont ME. Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1999 Jul; 261(6):917-32.
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Martin NP, Leavitt LM, Sommers CM, Dumont ME. Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments. Biochemistry. 1999 Jan 12; 38(2):682-95.
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Rebbeor JF, Connolly GC, Dumont ME, Ballatori N. ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins. J Biol Chem. 1998 Dec 11; 273(50):33449-54.
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Rebbeor JF, Connolly GC, Dumont ME, Ballatori N. ATP-dependent transport of reduced glutathione in yeast secretory vesicles. Biochem J. 1998 Sep 15; 334 ( Pt 3):723-9.
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Pollock WB, Rosell FI, Twitchett MB, Dumont ME, Mauk AG. Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition. Biochemistry. 1998 Apr 28; 37(17):6124-31.
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Sommers CM, Dumont ME. Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene. J Mol Biol. 1997 Feb 28; 266(3):559-75.
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Wang X, Dumont ME, Sherman F. Sequence requirements for mitochondrial import of yeast cytochrome c. J Biol Chem. 1996 Mar 22; 271(12):6594-604.
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Tavtigian SV, Simard J, Rommens J, Couch F, Shattuck-Eidens D, Neuhausen S, Merajver S, Thorlacius S, Offit K, Stoppa-Lyonnet D, Belanger C, Bell R, Berry S, Bogden R, Chen Q, Davis T, Dumont M, Frye C, Hattier T, Jammulapati S, Janecki T, Jiang P, Kehrer R, Leblanc JF, Mitchell JT, McArthur-Morrison J, Nguyen K, Peng Y, Samson C, Schroeder M, Snyder SC, Steele L, Stringfellow M, Stroup C, Swedlund B, Swense J, Teng D, Thomas A, Tran T, Tranchant M, Weaver-Feldhaus J, Wong AK, Shizuya H, Eyfjord JE, Cannon-Albright L, Tranchant M, Labrie F, Skolnick MH, Weber B, Kamb A, Goldgar DE. The complete BRCA2 gene and mutations in chromosome 13q-linked kindreds. Nat Genet. 1996 Mar; 12(3):333-7.
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Dumont ME, Corin AF, Campbell GA. Noncovalent binding of heme induces a compact apocytochrome c structure. Biochemistry. 1994 Jun 14; 33(23):7368-78.
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Dumont ME, Schlichter JB, Cardillo TS, Hayes MK, Bethlendy G, Sherman F. CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c. Mol Cell Biol. 1993 Oct; 13(10):6442-51.
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Dumont ME, Cardillo TS, Hayes MK, Sherman F. Role of cytochrome c heme lyase in mitochondrial import and accumulation of cytochrome c in Saccharomyces cerevisiae. Mol Cell Biol. 1991 Nov; 11(11):5487-96.
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Lachance Y, Luu-The V, Labrie C, Simard J, Dumont M, de Launoit Y, Guérin S, Leblanc G, Labrie F. Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells. J Biol Chem. 1990 Nov 25; 265(33):20469-75.
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Dumont MD, Mathews AJ, Nall BT, Baim SB, Eustice DC, Sherman F. Differential stability of two apo-isocytochromes c in the yeast Saccharomyces cerevisiae. J Biol Chem. 1990 Feb 15; 265(5):2733-9.
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Dumont ME, Sherman F, Thomas DY. Protein targeting and protein secretion. Genome. 1989; 31(2):1109-10.
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Dumont ME, Ernst JF, Sherman F. Coupling of heme attachment to import of cytochrome c into yeast mitochondria. Studies with heme lyase-deficient mitochondria and altered apocytochromes c. J Biol Chem. 1988 Nov 5; 263(31):15928-37.
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Dumont ME, Richards FM. The pH-dependent conformational change of diphtheria toxin. J Biol Chem. 1988 Feb 5; 263(4):2087-97.
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Dumont ME, Dei-Cas E, Maurois P, Slomianny C, Prensier G, Houcke-Lecomte M, Vernes A, Camus D. [Histopathology of the liver and kidney during malaria: relation to malaria-induced dyslipoproteinemia]. Ann Parasitol Hum Comp. 1988; 63(3):171-83.
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Dumont ME, Ernst JF, Hampsey DM, Sherman F. Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae. EMBO J. 1987 Jan; 6(1):235-41.
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Dumont ME, Trewhella J, Engelman DM, Richards FM. Stability of transmembrane regions in bacteriorhodopsin studied by progressive proteolysis. J Membr Biol. 1985; 88(3):233-47.
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Dumont ME, Richards FM. Insertion of apocytochrome c into lipid vesicles. J Biol Chem. 1984 Apr 10; 259(7):4147-56.
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Dumont ME, Wiggins JW, Hayward SB. Location of platinum binding sites on bacteriorhodopsin by electron diffraction. Proc Natl Acad Sci U S A. 1981 May; 78(5):2947-51.
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