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Diane Piekut

TitleAssociate Professor
InstitutionSchool of Medicine and Dentistry
DepartmentNeurobiology and Anatomy
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 603
Rochester NY 14642
 
 Awards And Honors
      Manuel D. Goldman Prize for Excellence in Teaching Medical Gross Anatomy
      NSF Ad Hoc reviewer
      NIH Ad Hoc reviewer  | Neurology A Study Section
1977     Outstanding Young Women of America
1978     Mellon Foundation Award
1981     Outstanding Young Women of America
1984 - 1989NIH Research Career Development Award
2003 - 2005George W. Merck Dean's Teaching Fellow
2004     Commendation for Excellence in Medical Education
2006     Commendation for Excellence in Medical Education
2007     Faculty Marshall  | Univ. of Rochester Commencement Ceremony
 
 Overview
RESEARCH:

The focus of my research program is to determine the activation and plasticity of neurons, especially corticotropin releasing factor (CRF)-containing neurons, in response to a brain insult (i.e., stress, transient global forebrain ischemia, seizure). Current studies address 1) the effect of transient global ischemia and seizure on the neuroendocrine hypothalamus, particularly neural substrates involved in regulation of the pituitary-adrenal axis, and 2) the differential effects of acute and chronic stress on the regulation of specific mRNA and peptide expression in activated neurons. 3) In addition to its neuroendocrine function, CRF also functions as an excitatory neurotransmitter/neuromodulator in extrahypothalamic brain sites. Studies in this laboratory suggest that extrahypothalamic CRF is significantly increased following specific brain insults and may play a role in ischemic and seizure brain damage. Investigations, which address the association of CRF induction following a brain insult with neuropathological processes, the administration of a CRF receptor antagonist to protect against the neurodegeneration and direct neurotoxic effects of CRF on neurons, are being pursued. Elucidation of the mechanisms responsible for neuronal vulnerability to brain insults will aid in the development of therapeutic drug interventions which selectively target the neuronal population most susceptible to ischemic or seizure damage, and thereby protect against neurodegeneration. Immunocytochemical localization of neuropeptides /neurotransmitters, in situ hybridization of neuropeptide gene expression, and tissue culture techniques are being employed in this research.

PUBLICATIONS:

Pretel, S., C.D. Applegate and D.T. Piekut. Apoptotic and necrotic cell death following kindling induced seizures. Acta Histologica, 99:71-79, 1997.
Callahan, T.A. and D.T. Piekut. Differential FOS expression induced by IL-1ß and IL-6 in rat hypothalamus and pituitary gland. J. Neuroimmunol., 73:207-211, 1997.
Samoriski, G.M., D.T. Piekut, and C.D. Applegate. Differential spatial patterns of Fos induction following generalized clonic and generalized tonic seizures. Exp. Neurol., 143:255-268, 1997.
Kellogg, C.K., G.B. Awatramani and D.T. Piekut. Adolescent development alters stressor-induced FOS immunoreactivity in rat brain. Neurosci., 83: 681-689, 1998.
Samoriski, G.M., D.T. Piekut and C.D. Applegate. Regional analysis of the patterns of Fos induction in brain following a seizure: Dependence on seizure history and seizure phenotype. Neuroscience, 84:1209-1222, 1998.
Piekut, D.T. and B. Phipps. Increased corticotropin-releasing factor immunoreactivity in select brain sites following kainate elicited seizures, Brain Research, 781:100-113, 1998.
Blair, M.L., A. Want, J.A. Olschowka and D.T. Piekut. Role of paraventricular nucleus parvicellular neurons in the compensatory responses to graded hemorrhage, Am.J. Physiol., 275: R278-R285, 1998.
Callahan, T.A., J.A. Moynihan and D.T. Piekut. Central nervous system activation following peripheral chemical sympathectomy: implications for neural-immune interactions, Brain, Behavior and Immunity, 12:230-241, 1998.
Piekut, D.P. and B Phipps. (1999) Corticotropin-releasing factor immunolabeled fibers in brain regions with localized kainate neurotoxicity, Acta Neuropathology 98:622-628, 1999.
Blair, M. L., R. L. Parman-Jaworski, A. Want, and D. T. Piekut. Parabrachial nucleus modulates cardiovascular responses to blood loss. Am. J. Physiol. 280: R1141-48, 2001.
Jaworski, R.L., D. Piekut, and M.L. Blair. Pregnancy alters lateral parabrachial nucleus but not hypothalamic Fos expression following hypotensive hemorrhag Brain Research Bulletin, 57: 595-602, 2002.
A Hiwatashi (P). R.Sidhu, RK Lee, R Deguzman, DT Piekut, PA Westesson: Kyphoplasty increases vertebral body height more than vertebroplasty. A cadaveric study, Radiology 237 (3): 1115-1119, 2005.

 
 Selected Publications
List All   |   Timeline
  1. Hiwatashi A, Sidhu R, Lee RK, deGuzman RR, Piekut DT, Westesson PL. Kyphoplasty versus vertebroplasty to increase vertebral body height: a cadaveric study. Radiology. 2005 Dec; 237(3):1115-9.
    View in: PubMed
  2. Jaworski RL, Piekut D, Blair ML. Pregnancy alters lateral parabrachial nucleus but not hypothalamic Fos expression following hypotensive hemorrhage. Brain Res Bull. 2002 Mar 15; 57(5):595-602.
    View in: PubMed
  3. Blair ML, Jaworski RL, Want A, Piekut DT. Parabrachial nucleus modulates cardiovascular responses to blood loss. Am J Physiol Regul Integr Comp Physiol. 2001 Apr; 280(4):R1141-8.
    View in: PubMed
  4. Piekut DT, Phipps B. Corticotropin-releasing factor--immunolabeled fibers in brain regions with localized kainate neurotoxicity. Acta Neuropathol. 1999 Dec; 98(6):622-8.
    View in: PubMed
  5. Callahan TA, Moynihan JA, Piekut DT. Central nervous system activation following peripheral chemical sympathectomy: implications for neural-immune interactions. Brain Behav Immun. 1998 Sep; 12(3):230-41.
    View in: PubMed
  6. Blair ML, Want A, Olschowka JA, Piekut D. Role of paraventricular nucleus parvicellular neurons in the compensatory responses to graded hemorrhage. Am J Physiol. 1998 Jul; 275(1 Pt 2):R278-85.
    View in: PubMed
  7. Samoriski GM, Piekut DT, Applegate CD. Regional analysis of the spatial patterns of Fos induction in brain following flurothyl kindling. Neuroscience. 1998 Jun; 84(4):1209-22.
    View in: PubMed
  8. Kellogg CK, Awatramani GB, Piekut DT. Adolescent development alters stressor-induced Fos immunoreactivity in rat brain. Neuroscience. 1998 Apr; 83(3):681-9.
    View in: PubMed
  9. Piekut DT, Phipps B. Increased corticotropin-releasing factor immunoreactivity in select brain sites following kainate elicited seizures. Brain Res. 1998 Jan 19; 781(1-2):100-13.
    View in: PubMed
  10. Callahan TA, Piekut DT. Differential Fos expression induced by IL-1beta and IL-6 in rat hypothalamus and pituitary gland. J Neuroimmunol. 1997 Mar; 73(1-2):207-11.
    View in: PubMed
  11. Pretel S, Applegate CD, Piekut D. Apoptotic and necrotic cell death following kindling induced seizures. Acta Histochem. 1997 Mar; 99(1):71-9.
    View in: PubMed
  12. Samoriski GM, Piekut DT, Applegate CD. Differential spatial patterns of Fos induction following generalized clonic and generalized tonic seizures. Exp Neurol. 1997 Feb; 143(2):255-68.
    View in: PubMed
  13. Piekut D, Phipps B, Pretel S, Applegate C. Effects of generalized convulsive seizures on corticotropin-releasing factor neuronal systems. Brain Res. 1996 Dec 16; 743(1-2):63-9.
    View in: PubMed
  14. Piekut DT, Pretel S, Applegate CD. Activation of oxytocin-containing neurons of the paraventricular nucleus (PVN) following generalized seizures. Synapse. 1996 Aug; 23(4):312-20.
    View in: PubMed
  15. Piekut DT, Phipps B. Corticotropin-releasing factor induction in rat piriform cortex following kainate-elicited seizures. Neurosci Lett. 1996 May 3; 209(1):45-8.
    View in: PubMed
  16. Sun Q, Pretel S, Applegate CD, Piekut DT. Oxytocin and vasopressin mRNA expression in rat hypothalamus following kainic acid-induced seizures. Neuroscience. 1996 Mar; 71(2):543-54.
    View in: PubMed
  17. Blair ML, Piekut D, Want A, Olschowka JA. Role of the hypothalamic paraventricular nucleus in cardiovascular regulation. Clin Exp Pharmacol Physiol. 1996 Feb; 23(2):161-5.
    View in: PubMed
  18. Pretel S, Applegate CD, Piekut DT. The kindling-activated neuronal network: recruitment of somatostatin-synthesizing neurons. Brain Res Bull. 1996; 41(4):237-47.
    View in: PubMed
  19. Pretel S, Applegate CD, Piekut DT. Seizure-induced activation of enkephalin- and somatostatin-synthesizing neurons. Peptides. 1995; 16(5):951-7.
    View in: PubMed
  20. Applegate CD, Pretel S, Piekut DT. The substantia nigra pars reticulata, seizures and Fos expression. Epilepsy Res. 1995 Jan; 20(1):31-9.
    View in: PubMed
  21. Pretel S, Applegate CD, Piekut DT. Activation of somatostatin-synthesizing neurons in the hippocampal formation through kindling-induced seizures. Hippocampus. 1995; 5(1):40-51.
    View in: PubMed
  22. Pretel S, Piekut DT. Enkephalin, substance P, and serotonin axonal input to c-fos-like immunoreactive neurons of the rat spinal cord. Peptides. 1991 Nov-Dec; 12(6):1243-50.
    View in: PubMed
  23. Pretel S, Piekut DT. ACTH and enkephalin axonal input to paraventricular neurons containing c-fos-like immunoreactivity. Synapse. 1991 Jun; 8(2):100-6.
    View in: PubMed
  24. Pretel S, Piekut DT. Coexistence of CRF peptide and oxytocin mRNA in the paraventricular nucleus. Peptides. 1990 May-Jun; 11(3):621-4.
    View in: PubMed
  25. Pretel S, Piekut D. Coexistence of corticotropin-releasing factor and enkephalin in the paraventricular nucleus of the rat. J Comp Neurol. 1990 Apr 8; 294(2):192-201.
    View in: PubMed
  26. Hornby PJ, Piekut DT. Distribution of catecholamine-synthesizing enzymes in goldfish brains: presumptive dopamine and norepinephrine neuronal organization. Brain Behav Evol. 1990; 35(1):49-64.
    View in: PubMed
  27. Berlove DJ, Piekut DT. Co-localization of putative vasopressin receptors and vasopressinergic neurons in rat hypothalamus. Histochemistry. 1990; 94(6):653-7.
    View in: PubMed
  28. Pretel S, Piekut DT. Mediation of changes in paraventricular vasopressin and oxytocin mRNA content to the medullary vagal complex and spinal cord of the rat. J Chem Neuroanat. 1989 Nov-Dec; 2(6):327-34.
    View in: PubMed
  29. Hornby PJ, Piekut DT. Opiocortin and catecholamine input to CRF-immunoreactive neurons in rat forebrain. Peptides. 1989 Nov-Dec; 10(6):1139-46.
    View in: PubMed
  30. Berlove DJ, Piekut DT. Vasopressin receptor distribution in adrenalectomized rats using vasopressin anti-idiotype. Peptides. 1989 Jul-Aug; 10(4):877-81.
    View in: PubMed
  31. Knigge KM, Piekut DT, Abood LG, Joseph SA, Michael GJ, Xin L, Berlove DJ. Immunocytochemistry of receptors using anti-idiotypic antibodies. Methods Enzymol. 1989; 178:212-21.
    View in: PubMed
  32. Knigge KM, Piekut DT, Berlove D. Immunocytochemistry of a vasopressin (AVP) receptor with anti-idiotype antibody: inhibition of staining with a peptide (PVA) encoded by an RNA that is complementary to AVP mRNA. Neurosci Lett. 1988 Apr 12; 86(3):269-71.
    View in: PubMed
  33. Hornby PJ, Piekut DT. Immunoreactive dopamine beta-hydroxylase in neuronal groups in the goldfish brain. Brain Behav Evol. 1988; 32(4):252-6.
    View in: PubMed
  34. Hornby PJ, Piekut DT. Anatomical evidence for interaction of ACTH1-39 immunostained fibers and hypothalamic paraventricular neurons that project to the dorsal vagal complex. Histochemistry. 1988; 90(3):201-6.
    View in: PubMed
  35. Hornby PJ, Piekut DT, Demski LS. Localization of immunoreactive tyrosine hydroxylase in the goldfish brain. J Comp Neurol. 1987 Jul 1; 261(1):1-14.
    View in: PubMed
  36. Hornby PJ, Piekut DT. Catecholamine distribution and relationship to magnocellular neurons in the paraventricular nucleus of the rat. Cell Tissue Res. 1987 May; 248(2):239-46.
    View in: PubMed
  37. Knigge KM, Piekut DT, Berlove DJ, Junig JT, Melrose PA. Staining of magnocellular neurons of the supraoptic and paraventricular nuclei with vasopressin anti-idiotype antibody: a potential method for receptor immunocytochemistry. Brain Res. 1987 Apr; 388(1):69-78.
    View in: PubMed
  38. Piekut DT. Interactions of immunostained ACTH1-39 fibers and CRF neurons in the paraventricular nucleus of rat hypothalamus: application of avidin-glucose oxidase to dual immunostaining procedures. J Histochem Cytochem. 1987 Feb; 35(2):261-5.
    View in: PubMed
  39. Piekut DT, Joseph SA. Co-existence of CRF and vasopressin immunoreactivity in parvocellular paraventricular neurons of rat hypothalamus. Peptides. 1986 Sep-Oct; 7(5):891-8.
    View in: PubMed
  40. Piekut DT. Ultrastructural characteristics of peptidergic neurons using pre-embedding immunocytochemical methods. Am J Anat. 1986 Feb-Mar; 175(2-3):197-216.
    View in: PubMed
  41. Joseph SA, Piekut DT. Dual immunostaining procedure demonstrating neurotransmitter and neuropeptide codistribution in the same brain section. Am J Anat. 1986 Feb-Mar; 175(2-3):331-42, 356-61.
    View in: PubMed
  42. Knigge KM, Piekut DT, Berlove DJ. Immunocytochemistry of magnocellular neurons of supraoptic and paraventricular nuclei of normal and Brattleboro rats with vasopressin anti-idiotype antibody. Cell Tissue Res. 1986; 246(3):509-13.
    View in: PubMed
  43. Piekut DT, Joseph SA. Relationship of CRF-immunostained cells and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. Peptides. 1985 Sep-Oct; 6(5):873-82.
    View in: PubMed
  44. Piekut DT. Relationship of ACTH1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. Peptides. 1985 Sep-Oct; 6(5):883-90.
    View in: PubMed
  45. Knigge KM, Piekut DT. Distribution of CRF- and tyrosine hydroxylase-immunoreactive neurons in the brainstem of the domestic fowl (Gallus domesticus). Peptides. 1985 Jan-Feb; 6(1):97-101.
    View in: PubMed
  46. Piekut DT. Ultrastructural studies on the afferent synaptic input to oxytocin-containing neurons in the paraventricular nucleus of the hypothalamus. J Ultrastruct Res. 1985 Jan; 90(1):71-9.
    View in: PubMed
  47. Knigge KM, Piekut DT, Joseph SA. The alpha MSH-specific and arcuate pro-opiomelanocortin neuronal systems of the hypothalamus: observations on their connectivity and response to colchicine treatment. Psychopharmacol Bull. 1985; 21(3):472-5.
    View in: PubMed
  48. Piekut DT, Knigge KM. Relationship of alpha MSH-specific neurons to the arcuate opiocortin neuronal system as determined by dual antigen immunocytochemical procedures. Peptides. 1984 Nov-Dec; 5(6):1089-95.
    View in: PubMed
  49. Piekut DT. Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) and LHRH-like material in brain. Influence of fixation solution at various pH values. J Histochem Cytochem. 1983 Nov; 31(11):1329-32.
    View in: PubMed
  50. Piekut DT, Casey SM. Penetration of immunoreagents in Vibratome-sectioned brain: a light and electron microscopic study. J Histochem Cytochem. 1983 May; 31(5):669-74.
    View in: PubMed
  51. Piekut DT. Ultrastructural characteristics of vasopressin-containing neurons in the paraventricular nucleus of the hypothalamus. Cell Tissue Res. 1983; 234(1):125-34.
    View in: PubMed
  52. Piekut DT, Knigge KM. Immunocytochemical analysis of the rat pineal gland using antisera generated against analogs of luteinizing hormone-releasing hormone (LHRH). J Histochem Cytochem. 1982 Feb; 30(2):106-10.
    View in: PubMed
  53. Piekut DT. Postnatal appearance of luteinizing hormone-releasing hormone (LHRH)-like material in the pineal gland of the rat. Cell Tissue Res. 1982; 223(3):695-8.
    View in: PubMed
  54. Piekut DT, Knigge KM. Immunocytochemical analysis of the rat pineal gland using antisera generated against luteinizing hormone-releasing hormone (LHRH). J Histochem Cytochem. 1981 May; 29(5):616-22.
    View in: PubMed
  55. Joseph SA, Piekut DT, Knigge KM. Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in Vibratome-sectioned brain. J Histochem Cytochem. 1981 Feb; 29(2):247-54.
    View in: PubMed
  56. Piekut DT, Knigge KM. Primary cultures of dispersed cells of rat pineal gland. I. Fine structure and indole metabolism. Cell Tissue Res. 1978 Apr 17; 188(2):285-97.
    View in: PubMed

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