Paul Kingsley
| Title | Research Assistant Professor |
|---|
| Institution | School of Medicine and Dentistry |
|---|
| Department | Pediatrics |
|---|
| Address | University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 703
Rochester NY 14642
|
|---|
|
|
|
| Title | Research Assistant Professor |
|---|
| Institution | University of Rochester Medical Center |
|---|
| Department | Center for Pediatric Biomedical Research |
|---|
|
|
|---|
|
Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal
Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.
An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.
-
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J. EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress. Blood. 2012 Sep 20; 120(12):2501-11.
-
McGrath KE, Frame JM, Fromm GJ, Koniski AD, Kingsley PD, Little J, Bulger M, Palis J. A transient definitive erythroid lineage with unique regulation of the ß-globin locus in the mammalian embryo. Blood. 2011 Apr 28; 117(17):4600-8.
-
Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M. An embryonic stage-specific enhancer within the murine ß-globin locus mediates domain-wide histone hyperacetylation. Blood. 2011 May 12; 117(19):5207-14.
-
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Frame JM, Koniski AD, Chen Y, Williams JP, McGrath KE, Dertinger SD, Palis J. Sublethal radiation injury uncovers a functional transition during erythroid maturation. Exp Hematol. 2011 Apr; 39(4):434-45.
-
Palis J, Malik J, McGrath KE, Kingsley PD. Primitive erythropoiesis in the mammalian embryo. Int J Dev Biol. 2010; 54(6-7):1011-8.
-
Nilsson R, Schultz IJ, Pierce EL, Soltis KA, Naranuntarat A, Ward DM, Baughman JM, Paradkar PN, Kingsley PD, Culotta VC, Kaplan J, Palis J, Paw BH, Mootha VK. Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis. Cell Metab. 2009 Aug; 10(2):119-30.
-
Naik AA, Xie C, Zuscik MJ, Kingsley P, Schwarz EM, Awad H, Guldberg R, Drissi H, Puzas JE, Boyce B, Zhang X, O'Keefe RJ. Reduced COX-2 expression in aged mice is associated with impaired fracture healing. J Bone Miner Res. 2009 Feb; 24(2):251-64.
-
Bullard T, Koek L, Roztocil E, Kingsley PD, Mirels L, Ovitt CE. Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands. Dev Biol. 2008 Aug 1; 320(1):72-8.
-
Keel SB, Doty RT, Yang Z, Quigley JG, Chen J, Knoblaugh S, Kingsley PD, De Domenico I, Vaughn MB, Kaplan J, Palis J, Abkowitz JL. A heme export protein is required for red blood cell differentiation and iron homeostasis. Science. 2008 Feb 8; 319(5864):825-8.
-
McGrath KE, Kingsley PD, Koniski AD, Porter RL, Bushnell TP, Palis J. Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus. Blood. 2008 Feb 15; 111(4):2409-17.
-
Yi Z, Cohen-Barak O, Hagiwara N, Kingsley PD, Fuchs DA, Erickson DT, Epner EM, Palis J, Brilliant MH. Sox6 directly silences epsilon globin expression in definitive erythropoiesis. PLoS Genet. 2006 Feb; 2(2):e14.
-
Kingsley PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, Palis J. "Maturational" globin switching in primary primitive erythroid cells. Blood. 2006 Feb 15; 107(4):1665-72.
-
Wingert RA, Galloway JL, Barut B, Foott H, Fraenkel P, Axe JL, Weber GJ, Dooley K, Davidson AJ, Schmid B, Schmidt B, Paw BH, Shaw GC, Kingsley P, Palis J, Schubert H, Chen O, Kaplan J, Zon LI. Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis. Nature. 2005 Aug 18; 436(7053):1035-39.
-
Smith N, Dong Y, Lian JB, Pratap J, Kingsley PD, van Wijnen AJ, Stein JL, Schwarz EM, O'Keefe RJ, Stein GS, Drissi MH. Overlapping expression of Runx1(Cbfa2) and Runx2(Cbfa1) transcription factors supports cooperative induction of skeletal development. J Cell Physiol. 2005 Apr; 203(1):133-43.
-
Ransom DG, Bahary N, Niss K, Traver D, Burns C, Trede NS, Paffett-Lugassy N, Saganic WJ, Lim CA, Hersey C, Zhou Y, Barut BA, Lin S, Kingsley PD, Palis J, Orkin SH, Zon LI. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004 Aug; 2(8):E237.
-
Kingsley PD, Malik J, Fantauzzo KA, Palis J. Yolk sac-derived primitive erythroblasts enucleate during mammalian embryogenesis. Blood. 2004 Jul 1; 104(1):19-25.
-
Davidson AJ, Ernst P, Wang Y, Dekens MP, Kingsley PD, Palis J, Korsmeyer SJ, Daley GQ, Zon LI. cdx4 mutants fail to specify blood progenitors and can be rescued by multiple hox genes. Nature. 2003 Sep 18; 425(6955):300-6.
-
Chung YS, Zhang WJ, Arentson E, Kingsley PD, Palis J, Choi K. Lineage analysis of the hemangioblast as defined by FLK1 and SCL expression. Development. 2002 Dec; 129(23):5511-20.
-
Lacaud G, Gore L, Kennedy M, Kouskoff V, Kingsley P, Hogan C, Carlsson L, Speck N, Palis J, Keller G. Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitro. Blood. 2002 Jul 15; 100(2):458-66.
-
Elble RC, Ji G, Nehrke K, DeBiasio J, Kingsley PD, Kotlikoff MI, Pauli BU. Molecular and functional characterization of a murine calcium-activated chloride channel expressed in smooth muscle. J Biol Chem. 2002 May 24; 277(21):18586-91.
-
Lan Y, Kingsley PD, Cho ES, Jiang R. Osr2, a new mouse gene related to Drosophila odd-skipped, exhibits dynamic expression patterns during craniofacial, limb, and kidney development. Mech Dev. 2001 Sep; 107(1-2):175-9.
-
Kingsley PD, McGrath KE, Maltby KM, Koniski AD, Ramchandran R, Palis J. Subtractive hybridization reveals tissue-specific expression of ahnak during embryonic development. Dev Growth Differ. 2001 Apr; 43(2):133-43.
-
Ten Hagen KG, Bedi GS, Tetaert D, Kingsley PD, Hagen FK, Balys MM, Beres TM, Degand P, Tabak LA. Cloning and characterization of a ninth member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, ppGaNTase-T9. J Biol Chem. 2001 May 18; 276(20):17395-404.
-
Kingsley PD, Hagen KG, Maltby KM, Zara J, Tabak LA. Diverse spatial expression patterns of UDP-GalNAc:polypeptide N-acetylgalactosaminyl-transferase family member mRNAs during mouse development. Glycobiology. 2000 Dec; 10(12):1317-23.
-
Chi H, Yang X, Kingsley PD, O'Keefe RJ, Puzas JE, Rosier RN, Shears SB, Reynolds PR. Targeted deletion of Minpp1 provides new insight into the activity of multiple inositol polyphosphate phosphatase in vivo. Mol Cell Biol. 2000 Sep; 20(17):6496-507.
-
Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI. Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature. 2000 Feb 17; 403(6771):776-81.
-
Liu LQ, Ilaria R, Kingsley PD, Iwama A, van Etten RA, Palis J, Zhang DE. A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein AML1-ETO-expressing mice, functions in hematopoietic cell differentiation. Mol Cell Biol. 1999 Apr; 19(4):3029-38.
-
Hetherington CJ, Kingsley PD, Crocicchio F, Zhang P, Rabin MS, Palis J, Zhang DE. Characterization of human endotoxin lipopolysaccharide receptor CD14 expression in transgenic mice. J Immunol. 1999 Jan 1; 162(1):503-9.
-
Kingsley PD, Whitin JC, Cohen HJ, Palis J. Developmental expression of extracellular glutathione peroxidase suggests antioxidant roles in deciduum, visceral yolk sac, and skin. Mol Reprod Dev. 1998 Apr; 49(4):343-55.
-
Varade WS, Carnahan JA, Kingsley PD, Insel RA. Inherent properties of somatic hypermutation as revealed by human non-productive VH6 immunoglobulin rearrangements. Immunology. 1998 Feb; 93(2):171-6.
-
Miller RN, Dalamagas DG, Kingsley PD, Ettensohn CA. Expression of S9 and actin CyIIa mRNAs reveals dorso-ventral polarity and mesodermal sublineages in the vegetal plate of the sea urchin embryo. Mech Dev. 1996 Nov; 60(1):3-12.
-
Palis J, Kingsley PD. Differential gene expression during early murine yolk sac development. Mol Reprod Dev. 1995 Sep; 42(1):19-27.
-
Palis J, McGrath KE, Kingsley PD. Initiation of hematopoiesis and vasculogenesis in murine yolk sac explants. Blood. 1995 Jul 1; 86(1):156-63.
-
Kingsley PD, Palis J. GRP2 proteins contain both CCHC zinc fingers and a cold shock domain. Plant Cell. 1994 Nov; 6(11):1522-3.
-
Kingsley PD, Angerer LM, Angerer RC. Major temporal and spatial patterns of gene expression during differentiation of the sea urchin embryo. Dev Biol. 1993 Jan; 155(1):216-34.
-
Angerer LM, Yang Q, Liesveld J, Kingsley PD, Angerer RC. Tissue-restricted accumulation of a ribosomal protein mRNA is not coordinated with rRNA transcription and precedes growth of the sea urchin pluteus larva. Dev Biol. 1992 Jan; 149(1):27-40.
-
Futterweit W, Dunaif A, Yeh HC, Kingsley P. The prevalence of hyperandrogenism in 109 consecutive female patients with diffuse alopecia. J Am Acad Dermatol. 1988 Nov; 19(5 Pt 1):831-6.
-
Daley ML, Gallo AE, Gehling GF, Isom JB, Mauch W, Kingsley PD. Fluctuation of intracranial pressure associated with the cardiac cycle. Neurosurgery. 1982 Nov; 11(5):617-21.
|
Kingsley's Networks
Concepts  Derived automatically from this person's publications. _
Co-Authors People in Profiles who have published with this person. _
Similar People People who share similar concepts with this person. _
Same Department
People who are also in this person's primary department.
|
