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Alan Smrcka

TitleProfessor
InstitutionSchool of Medicine and Dentistry
DepartmentPharmacology and Physiology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester NY 14642
Other Positions
TitleProfessor
InstitutionSchool of Medicine and Dentistry
DepartmentBiochemistry and Biophysics

TitleProfessor
InstitutionUniversity of Rochester Medical Center
DepartmentAab Cardiovascular Research Institute

 
 Awards And Honors
2007     Davey Award  | URMC Cancer Center
 
 Overview
G protein coupled receptors (GPCRs) form a large family of cell surface receptors responsible for triggering cellular responses to a variety of extracellular stimuli including drugs such as opiates, and hormones such as adrenaline, serotonin or acetylcholine. All GPCRs function through activation of trimeric G proteins located on the inner surface of the plasma membrane. Activated G proteins target ion channels or enzymes that produce second messengers with a variety of effects depending on the type of cell that is stimulated. Examples include regulation of synaptic transmission in the central nervous system, chemotaxis in the immune system, and vascular remodeling in the cardiovascular system. This family of receptors is an important target for pharmaceuticals and defects in GPCR systems are responsible for a number of diseases.

Our laboratory focuses on analysis of the interactions between the G proteins and their protein targets at a molecular and structural level with the goal of understanding how these interactions lead to alterations in protein and cellular activities. Another goal is to connect the biochemical information about protein interaction interfaces to specific cellular physiologies. To this end we are developing antagonists of specific G protein interactions and using these tools to probe the functions of those interactions in living cells. This approach will help to define the roles of specific G protein interactions in physiological processes and as potential targets for therapeutic intervention in cardiovascular disease or cancer.

 
 Selected Publications
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  1. Kan W, Adjobo-Hermans M, Burroughs M, Faibis G, Malik S, Tall GG, Smrcka AV. M3 Muscarinic Receptor Interaction with Phospholipase C ß3 Determines Its Signaling Efficiency. J Biol Chem. 2014 Apr 18; 289(16):11206-18.
    View in: PubMed
  2. Xiang SY, Ouyang K, Yung BS, Miyamoto S, Smrcka AV, Chen J, Brown JH. PLC{varepsilon}, PKD1, and SSH1L Transduce RhoA Signaling to Protect Mitochondria from Oxidative Stress in the Heart. Sci Signal. 2013; 6(306):ra108.
    View in: PubMed
  3. Ruisanchez E, Dancs P, Kerék M, Németh T, Faragó B, Balogh A, Patil R, Jennings BL, Liliom K, Malik KU, Smrcka AV, Tigyi G, Benyó Z. Lysophosphatidic acid induces vasodilation mediated by LPA1 receptors, phospholipase C, and endothelial nitric oxide synthase. FASEB J. 2014 Feb; 28(2):880-90.
    View in: PubMed
  4. Easterhoff D, Dimaio JT, Liyanage W, Lo CW, Bae W, Doran TM, Smrcka A, Nilsson BL, Dewhurst S. Fluorescence detection of cationic amyloid fibrils in human semen. Bioorg Med Chem Lett. 2013 Sep 15; 23(18):5199-202.
    View in: PubMed
  5. Sun Z, Smrcka AV, Chen S. WDR26 Functions as a Scaffolding Protein to Promote Gß?-mediated Phospholipase C ß2 (PLCß2) Activation in Leukocytes. J Biol Chem. 2013 Jun 7; 288(23):16715-25.
    View in: PubMed
  6. Smrcka AV. Molecular targeting of Ga and Gß? subunits: a potential approach for cancer therapeutics. Trends Pharmacol Sci. 2013 May; 34(5):290-8.
    View in: PubMed
  7. Zhang L, Malik S, Pang J, Wang H, Park KM, Yule DI, Blaxall BC, Smrcka AV. Phospholipase ce hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy. Cell. 2013 Mar 28; 153(1):216-27.
    View in: PubMed
  8. Dusaban SS, Purcell NH, Rockenstein E, Masliah E, Cho MK, Smrcka AV, Brown JH. Phospholipase C{varepsilon} links G protein-coupled receptor activation to inflammatory astrocytic responses. Proc Natl Acad Sci U S A. 2013 Feb 26; 110(9):3609-14.
    View in: PubMed
  9. Dbouk HA, Vadas O, Shymanets A, Burke JE, Salamon RS, Khalil BD, Barrett MO, Waldo GL, Surve C, Hsueh C, Perisic O, Harteneck C, Shepherd PR, Harden TK, Smrcka AV, Taussig R, Bresnick AR, Nürnberg B, Williams RL, Backer JM. G Protein-Coupled Receptor-Mediated Activation of p110ß by Gß? Is Required for Cellular Transformation and Invasiveness. Sci Signal. 2012; 5(253):ra89.
    View in: PubMed
  10. McCoy KL, Gyoneva S, Vellano CP, Smrcka AV, Traynelis SF, Hepler JR. Protease-activated receptor 1 (PAR1) coupling to G(q/11) but not to G(i/o) or G(12/13) is mediated by discrete amino acids within the receptor second intracellular loop. Cell Signal. 2012 Jun; 24(6):1351-60.
    View in: PubMed
  11. Smrcka AV, Brown JH, Holz GG. Role of phospholipase Ce in physiological phosphoinositide signaling networks. Cell Signal. 2012 Jun; 24(6):1333-43.
    View in: PubMed
  12. Lin Y, Smrcka AV. Understanding molecular recognition by G protein ß? subunits on the path to pharmacological targeting. Mol Pharmacol. 2011 Oct; 80(4):551-7.
    View in: PubMed
  13. Seneviratne AM, Burroughs M, Giralt E, Smrcka AV. Direct-reversible binding of small molecules to G protein ß? subunits. Biochim Biophys Acta. 2011 Sep; 1814(9):1210-8.
    View in: PubMed
  14. Zhang L, Malik S, Kelley GG, Kapiloff MS, Smrcka AV. Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes. J Biol Chem. 2011 Jul 1; 286(26):23012-21.
    View in: PubMed
  15. Dzhura I, Chepurny OG, Leech CA, Roe MW, Dzhura E, Xu X, Lu Y, Schwede F, Genieser HG, Smrcka AV, Holz GG. Phospholipase C-e links Epac2 activation to the potentiation of glucose-stimulated insulin secretion from mouse islets of Langerhans. Islets. 2011 May-Jun; 3(3):121-8.
    View in: PubMed
  16. Park MS, Smrcka AV, Stern HA. Conformational flexibility and binding interactions of the G protein ß? heterodimer. Proteins. 2011 Feb; 79(2):518-27.
    View in: PubMed
  17. Kamal FA, Smrcka AV, Blaxall BC. Taking the heart failure battle inside the cell: small molecule targeting of Gß? subunits. J Mol Cell Cardiol. 2011 Oct; 51(4):462-7.
    View in: PubMed
  18. Chan P, Gabay M, Wright FA, Kan W, Oner SS, Lanier SM, Smrcka AV, Blumer JB, Tall GG. Purification of heterotrimeric G protein alpha subunits by GST-Ric-8 association: primary characterization of purified G alpha(olf). J Biol Chem. 2011 Jan 28; 286(4):2625-35.
    View in: PubMed
  19. Dzhura I, Chepurny OG, Kelley GG, Leech CA, Roe MW, Dzhura E, Afshari P, Malik S, Rindler MJ, Xu X, Lu Y, Smrcka AV, Holz GG. Epac2-dependent mobilization of intracellular Ca²+ by glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in ß-cells of phospholipase C-e knockout mice. J Physiol. 2010 Dec 15; 588(Pt 24):4871-89.
    View in: PubMed
  20. Gaudy AM, Clementi AH, Campbell JS, Smrcka AV, Mooney RA. Suppressor of cytokine signaling-3 is a glucagon-inducible inhibitor of PKA activity and gluconeogenic gene expression in hepatocytes. J Biol Chem. 2010 Dec 31; 285(53):41356-65.
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  21. Dessal AL, Prades R, Giralt E, Smrcka AV. Rational design of a selective covalent modifier of G protein ß? subunits. Mol Pharmacol. 2011 Jan; 79(1):24-33.
    View in: PubMed
  22. Casey LM, Pistner AR, Belmonte SL, Migdalovich D, Stolpnik O, Nwakanma FE, Vorobiof G, Dunaevsky O, Matavel A, Lopes CM, Smrcka AV, Blaxall BC. Small molecule disruption of G beta gamma signaling inhibits the progression of heart failure. Circ Res. 2010 Aug 20; 107(4):532-9.
    View in: PubMed
  23. Smrcka AV, Kichik N, Tarragó T, Burroughs M, Park MS, Itoga NK, Stern HA, Willardson BM, Giralt E. NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Proc Natl Acad Sci U S A. 2010 Jan 12; 107(2):639-44.
    View in: PubMed
  24. Serasinghe MN, Seneviratne AM, Smrcka AV, Yoon Y. Identification and characterization of unique proline-rich peptides binding to the mitochondrial fission protein hFis1. J Biol Chem. 2010 Jan 1; 285(1):620-30.
    View in: PubMed
  25. Wang Z, Kumamoto Y, Wang P, Gan X, Lehmann D, Smrcka AV, Cohn L, Iwasaki A, Li L, Wu D. Regulation of immature dendritic cell migration by RhoA guanine nucleotide exchange factor Arhgef5. J Biol Chem. 2009 Oct 16; 284(42):28599-606.
    View in: PubMed
  26. Bianchi E, Norcini M, Smrcka A, Ghelardini C. Supraspinal Gbetagamma-dependent stimulation of PLCbeta originating from G inhibitory protein-mu opioid receptor-coupling is necessary for morphine induced acute hyperalgesia. J Neurochem. 2009 Oct; 111(1):171-80.
    View in: PubMed
  27. Park MS, Dessal AL, Smrcka AV, Stern HA. Evaluating docking methods for prediction of binding affinities of small molecules to the G protein betagamma subunits. J Chem Inf Model. 2009 Feb; 49(2):437-43.
    View in: PubMed
  28. Mathews JL, Smrcka AV, Bidlack JM. A novel Gbetagamma-subunit inhibitor selectively modulates mu-opioid-dependent antinociception and attenuates acute morphine-induced antinociceptive tolerance and dependence. J Neurosci. 2008 Nov 19; 28(47):12183-9.
    View in: PubMed
  29. Oestreich EA, Malik S, Goonasekera SA, Blaxall BC, Kelley GG, Dirksen RT, Smrcka AV. Epac and phospholipase Cepsilon regulate Ca2+ release in the heart by activation of protein kinase Cepsilon and calcium-calmodulin kinase II. J Biol Chem. 2009 Jan 16; 284(3):1514-22.
    View in: PubMed
  30. Smrcka AV. G protein ß? subunits: central mediators of G protein-coupled receptor signaling. Cell Mol Life Sci. 2008 Jul; 65(14):2191-214.
    View in: PubMed
  31. Smrcka AV, Lehmann DM, Dessal AL. G protein betagamma subunits as targets for small molecule therapeutic development. Comb Chem High Throughput Screen. 2008 Jun; 11(5):382-95.
    View in: PubMed
  32. Lehmann DM, Seneviratne AM, Smrcka AV. Small molecule disruption of G protein beta gamma subunit signaling inhibits neutrophil chemotaxis and inflammation. Mol Pharmacol. 2008 Feb; 73(2):410-8.
    View in: PubMed
  33. Smrcka AV, Oestreich EA, Blaxall BC, Dirksen RT. EPAC regulation of cardiac EC coupling. J Physiol. 2007 Nov 1; 584(Pt 3):1029-31.
    View in: PubMed
  34. Citro S, Malik S, Oestreich EA, Radeff-Huang J, Kelley GG, Smrcka AV, Brown JH. Phospholipase Cepsilon is a nexus for Rho and Rap-mediated G protein-coupled receptor-induced astrocyte proliferation. Proc Natl Acad Sci U S A. 2007 Sep 25; 104(39):15543-8.
    View in: PubMed
  35. Rosenzweig DH, Nair KS, Wei J, Wang Q, Garwin G, Saari JC, Chen CK, Smrcka AV, Swaroop A, Lem J, Hurley JB, Slepak VZ. Subunit dissociation and diffusion determine the subcellular localization of rod and cone transducins. J Neurosci. 2007 May 16; 27(20):5484-94.
    View in: PubMed
  36. Yuan C, Sato M, Lanier SM, Smrcka AV. Signaling by a non-dissociated complex of G protein ß? and a subunits stimulated by a receptor-independent activator of G protein signaling, AGS8. J Biol Chem. 2007 Jul 6; 282(27):19938-47.
    View in: PubMed
  37. Lehmann DM, Yuan C, Smrcka AV. Analysis and pharmacological targeting of phospholipase C beta interactions with G proteins. Methods Enzymol. 2007; 434:29-48.
    View in: PubMed
  38. Oestreich EA, Wang H, Malik S, Kaproth-Joslin KA, Blaxall BC, Kelley GG, Dirksen RT, Smrcka AV. Epac-mediated activation of phospholipase C(epsilon) plays a critical role in beta-adrenergic receptor-dependent enhancement of Ca2+ mobilization in cardiac myocytes. J Biol Chem. 2007 Feb 23; 282(8):5488-95.
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  39. Blumer JB, Smrcka AV, Lanier SM. Mechanistic pathways and biological roles for receptor-independent activators of G-protein signaling. Pharmacol Ther. 2007 Mar; 113(3):488-506.
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  40. Hinkes B, Wiggins RC, Gbadegesin R, Vlangos CN, Seelow D, Nürnberg G, Garg P, Verma R, Chaib H, Hoskins BE, Ashraf S, Becker C, Hennies HC, Goyal M, Wharram BL, Schachter AD, Mudumana S, Drummond I, Kerjaschki D, Waldherr R, Dietrich A, Ozaltin F, Bakkaloglu A, Cleper R, Basel-Vanagaite L, Pohl M, Griebel M, Tsygin AN, Soylu A, Müller D, Sorli CS, Bunney TD, Katan M, Liu J, Attanasio M, O'toole JF, Hasselbacher K, Mucha B, Otto EA, Airik R, Kispert A, Kelley GG, Smrcka AV, Gudermann T, Holzman LB, Nürnberg P, Hildebrandt F. Positional cloning uncovers mutations in PLCE1 responsible for a nephrotic syndrome variant that may be reversible. Nat Genet. 2006 Dec; 38(12):1397-405.
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  41. Bonacci TM, Mathews JL, Yuan C, Lehmann DM, Malik S, Wu D, Font JL, Bidlack JM, Smrcka AV. Differential targeting of Gbetagamma-subunit signaling with small molecules. Science. 2006 Apr 21; 312(5772):443-6.
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  42. Sato M, Cismowski MJ, Toyota E, Smrcka AV, Lucchesi PA, Chilian WM, Lanier SM. Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with Gbetagamma. Proc Natl Acad Sci U S A. 2006 Jan 17; 103(3):797-802.
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  43. Kelley GG, Kaproth-Joslin KA, Reks SE, Smrcka AV, Wojcikiewicz RJ. G-protein-coupled receptor agonists activate endogenous phospholipase Cepsilon and phospholipase Cbeta3 in a temporally distinct manner. J Biol Chem. 2006 Feb 3; 281(5):2639-48.
    View in: PubMed
  44. Wang H, Oestreich EA, Maekawa N, Bullard TA, Vikstrom KL, Dirksen RT, Kelley GG, Blaxall BC, Smrcka AV. Phospholipase C epsilon modulates beta-adrenergic receptor-dependent cardiac contraction and inhibits cardiac hypertrophy. Circ Res. 2005 Dec 9; 97(12):1305-13.
    View in: PubMed
  45. Mahon MJ, Bonacci TM, Divieti P, Smrcka AV. A docking site for G protein ß? subunits on the parathyroid hormone 1 receptor supports signaling through multiple pathways. Mol Endocrinol. 2006 Jan; 20(1):136-46.
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  46. Davis TL, Bonacci TM, Sprang SR, Smrcka AV. Structural and molecular characterization of a preferred protein interaction surface on G protein beta gamma subunits. Biochemistry. 2005 Aug 9; 44(31):10593-604.
    View in: PubMed
  47. Malik S, Ghosh M, Bonacci TM, Tall GG, Smrcka AV. Ric-8 enhances G protein betagamma-dependent signaling in response to betagamma-binding peptides in intact cells. Mol Pharmacol. 2005 Jul; 68(1):129-36.
    View in: PubMed
  48. Bonacci TM, Ghosh M, Malik S, Smrcka AV. Regulatory interactions between the amino terminus of G-protein betagamma subunits and the catalytic domain of phospholipase Cbeta2. J Biol Chem. 2005 Mar 18; 280(11):10174-81.
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  49. Shajahan AN, Tiruppathi C, Smrcka AV, Malik AB, Minshall RD. Gbetagamma activation of Src induces caveolae-mediated endocytosis in endothelial cells. J Biol Chem. 2004 Nov 12; 279(46):48055-62.
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  50. Kelley GG, Reks SE, Smrcka AV. Hormonal regulation of phospholipase Cepsilon through distinct and overlapping pathways involving G12 and Ras family G-proteins. Biochem J. 2004 Feb 15; 378(Pt 1):129-39.
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  51. Ghosh M, Smrcka AV. Assay for G protein-dependent activation of phospholipase C beta using purified protein components. Methods Mol Biol. 2004; 237:67-75.
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  52. Ghosh M, Wang H, Kelley GG, Smrcka AV. Purification of phospholipase C beta and phospholipase C epsilon from Sf9 cells. Methods Mol Biol. 2004; 237:55-64.
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  53. Li Z, Hannigan M, Mo Z, Liu B, Lu W, Wu Y, Smrcka AV, Wu G, Li L, Liu M, Huang CK, Wu D. Directional sensing requires G beta gamma-mediated PAK1 and PIX alpha-dependent activation of Cdc42. Cell. 2003 Jul 25; 114(2):215-27.
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  54. Ghosh M, Peterson YK, Lanier SM, Smrcka AV. Receptor- and nucleotide exchange-independent mechanisms for promoting G protein subunit dissociation. J Biol Chem. 2003 Sep 12; 278(37):34747-50.
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  55. Goubaeva F, Ghosh M, Malik S, Yang J, Hinkle PM, Griendling KK, Neubig RR, Smrcka AV. Stimulation of cellular signaling and G protein subunit dissociation by G protein betagamma subunit-binding peptides. J Biol Chem. 2003 May 30; 278(22):19634-41.
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  56. Smrcka AV, Scott JK. Discovery of ligands for beta gamma subunits from phage-displayed peptide libraries. Methods Enzymol. 2002; 344:557-76.
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  57. Fogg VC, Azpiazu I, Linder ME, Smrcka A, Scarlata S, Gautam N. Role of the gamma subunit prenyl moiety in G protein beta gamma complex interaction with phospholipase Cbeta. J Biol Chem. 2001 Nov 9; 276(45):41797-802.
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  58. Kelley GG, Reks SE, Ondrako JM, Smrcka AV. Phospholipase C(epsilon): a novel Ras effector. EMBO J. 2001 Feb 15; 20(4):743-54.
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  59. Scott JK, Huang SF, Gangadhar BP, Samoriski GM, Clapp P, Gross RA, Taussig R, Smrcka AV. Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein betagamma subunits is used for recognition of a subclass of effectors. EMBO J. 2001 Feb 15; 20(4):767-76.
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  60. Yoshikawa DM, Bresciano K, Hatwar M, Smrcka AV. Characterization of a phospholipase C beta 2-binding site near the amino-terminal coiled-coil of G protein beta gamma subunits. J Biol Chem. 2001 Apr 6; 276(14):11246-51.
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  61. Hou Y, Azpiazu I, Smrcka A, Gautam N. Selective role of G protein gamma subunits in receptor interaction. J Biol Chem. 2000 Dec 15; 275(50):38961-4.
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  62. Yoshikawa DM, Hatwar M, Smrcka AV. G protein beta 5 subunit interactions with alpha subunits and effectors. Biochemistry. 2000 Sep 19; 39(37):11340-7.
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  63. Barr AJ, Ali H, Haribabu B, Snyderman R, Smrcka AV. Identification of a region at the N-terminus of phospholipase C-beta 3 that interacts with G protein beta gamma subunits. Biochemistry. 2000 Feb 22; 39(7):1800-6.
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  64. Li Z, Jiang H, Xie W, Zhang Z, Smrcka AV, Wu D. Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction. Science. 2000 Feb 11; 287(5455):1046-9.
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  65. Xie W, Samoriski GM, McLaughlin JP, Romoser VA, Smrcka A, Hinkle PM, Bidlack JM, Gross RA, Jiang H, Wu D. Genetic alteration of phospholipase C beta3 expression modulates behavioral and cellular responses to mu opioids. Proc Natl Acad Sci U S A. 1999 Aug 31; 96(18):10385-90.
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  66. Sankaran B, Osterhout J, Wu D, Smrcka AV. Identification of a structural element in phospholipase C beta2 that interacts with G protein betagamma subunits. J Biol Chem. 1998 Mar 20; 273(12):7148-54.
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  67. Stephens LR, Eguinoa A, Erdjument-Bromage H, Lui M, Cooke F, Coadwell J, Smrcka AS, Thelen M, Cadwallader K, Tempst P, Hawkins PT. The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101. Cell. 1997 Apr 4; 89(1):105-14.
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  68. Ptasznik A, Prossnitz ER, Yoshikawa D, Smrcka A, Traynor-Kaplan AE, Bokoch GM. A tyrosine kinase signaling pathway accounts for the majority of phosphatidylinositol 3,4,5-trisphosphate formation in chemoattractant-stimulated human neutrophils. J Biol Chem. 1996 Oct 11; 271(41):25204-7.
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  69. Romoser V, Ball R, Smrcka AV. Phospholipase C beta2 association with phospholipid interfaces assessed by fluorescence resonance energy transfer. G protein betagamma subunit-mediated translocation is not required for enzyme activation. J Biol Chem. 1996 Oct 11; 271(41):25071-8.
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  70. Parish CA, Smrcka AV, Rando RR. The role of G protein methylation in the function of a geranylgeranylated beta gamma isoform. Biochemistry. 1996 Jun 11; 35(23):7499-505.
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  71. Jiang H, Kuang Y, Wu Y, Smrcka A, Simon MI, Wu D. Pertussis toxin-sensitive activation of phospholipase C by the C5a and fMet-Leu-Phe receptors. J Biol Chem. 1996 Jun 7; 271(23):13430-4.
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  72. Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proc Natl Acad Sci U S A. 1996 Apr 2; 93(7):2964-8.
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  73. Parish CA, Smrcka AV, Rando RR. Functional significance of beta gamma-subunit carboxymethylation for the activation of phospholipase C and phosphoinositide 3-kinase. Biochemistry. 1995 Jun 13; 34(23):7722-7.
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  74. Stephens L, Smrcka A, Cooke FT, Jackson TR, Sternweis PC, Hawkins PT. A novel phosphoinositide 3 kinase activity in myeloid-derived cells is activated by G protein beta gamma subunits. Cell. 1994 Apr 8; 77(1):83-93.
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  75. Ueda N, Iñiguez-Lluhi JA, Lee E, Smrcka AV, Robishaw JD, Gilman AG. G protein beta gamma subunits. Simplified purification and properties of novel isoforms. J Biol Chem. 1994 Feb 11; 269(6):4388-95.
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  76. Pang IH, Smrcka AV, Sternweis PC. Synthesis and applications of affinity matrix containing immobilized beta gamma subunits of G proteins. Methods Enzymol. 1994; 237:164-74.
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  77. Kozasa T, Hepler JR, Smrcka AV, Simon MI, Rhee SG, Sternweis PC, Gilman AG. Purification and characterization of recombinant G16 alpha from Sf9 cells: activation of purified phospholipase C isozymes by G-protein alpha subunits. Proc Natl Acad Sci U S A. 1993 Oct 1; 90(19):9176-80.
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  78. Hepler JR, Kozasa T, Smrcka AV, Simon MI, Rhee SG, Sternweis PC, Gilman AG. Purification from Sf9 cells and characterization of recombinant Gq alpha and G11 alpha. Activation of purified phospholipase C isozymes by G alpha subunits. J Biol Chem. 1993 Jul 5; 268(19):14367-75.
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  79. Smrcka AV, Sternweis PC. Regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and beta gamma subunits. J Biol Chem. 1993 May 5; 268(13):9667-74.
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  80. Sternweis PC, Smrcka AV. G proteins in signal transduction: the regulation of phospholipase C. Ciba Found Symp. 1993; 176:96-106; discussion 106-11.
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  81. Sternweis PC, Smrcka AV. Regulation of phospholipase C by G proteins. Trends Biochem Sci. 1992 Dec; 17(12):502-6.
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  82. Sternweis PC, Smrcka AV, Gutowski S. Hormone signalling via G-protein: regulation of phosphatidylinositol 4,5-bisphosphate hydrolysis by Gq. Philos Trans R Soc Lond B Biol Sci. 1992 Apr 29; 336(1276):35-41; discussion 41-2.
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  83. Berstein G, Blank JL, Smrcka AV, Higashijima T, Sternweis PC, Exton JH, Ross EM. Reconstitution of agonist-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis using purified m1 muscarinic receptor, Gq/11, and phospholipase C-beta 1. J Biol Chem. 1992 Apr 25; 267(12):8081-8.
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  84. Gutowski S, Smrcka A, Nowak L, Wu DG, Simon M, Sternweis PC. Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones. J Biol Chem. 1991 Oct 25; 266(30):20519-24.
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  85. Wange RL, Smrcka AV, Sternweis PC, Exton JH. Photoaffinity labeling of two rat liver plasma membrane proteins with [32P]gamma-azidoanilido GTP in response to vasopressin. Immunologic identification as alpha subunits of the Gq class of G proteins. J Biol Chem. 1991 Jun 25; 266(18):11409-12.
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  86. Smrcka AV, Bohnert HJ, Jensen RG. Modulation of the tight binding of carboxyarabinitol 1,5-bisphosphate to the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys. 1991 Apr; 286(1):14-9.
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  87. Smrcka AV, Ramage RT, Bohnert HJ, Jensen RG. Purification and characterization of large and small subunits of ribulose 1,5-bisphosphate carboxylase expressed separately in Escherichia coli. Arch Biochem Biophys. 1991 Apr; 286(1):6-13.
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  88. Smrcka AV, Hepler JR, Brown KO, Sternweis PC. Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq. Science. 1991 Feb 15; 251(4995):804-7.
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  89. Smrcka AV, Jensen RG. HPLC separation and indirect ultraviolet detection of phosphorylated sugars. Plant Physiol. 1988 Feb; 86(2):615-8.
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  90. Smrcka AV, Szarek SR. Phenotypical temperature adaptation of protein turnover in desert annuals. Plant Physiol. 1986 Jan; 80(1):206-10.
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