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Martin Pavelka

TitleAssociate Professor
InstitutionSchool of Medicine and Dentistry
DepartmentMicrobiology and Immunology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 672
Rochester NY 14642
 
 Awards And Honors
1988     Graduated magna cum laude - Cleveland State University
1988     Doretta C. Thelker Award, Cleveland State University
1990 - 1993Public Heath Service NIH Predoctoral Trainee
1997     Recipient of a Burroughs Wellcome Fund Career Award in the Biomedical Sciences
2006     Awardee, URMC Excellence in Research
 
 Overview
1. Research Projects in Progress

My research expertise is in the genetics, physiology, and biochemistry of the cell envelopes of bacterial respiratory pathogens, with a special focus on the cell wall peptidoglycan.

Tuberculosis Research
1. Biosynthesis of the mycobacterial cell wall. Our current research activities focus on understanding the genetics and biochemistry of cell envelope biosynthesis in Mycobacterium tuberculosis and related mycobacteria with the goal of identifying new targets that can be translated into the development of novel anti-tuberculosis drugs. Our studies focus on four aspects of the peptidoglycan of mycobacteria: 1) identifying the enzymes responsible for DAP-DAP cross links in the cell wall and their importance for cell survival under non-replicating conditions, 2) understanding the role of carbohydrate hydroxylation and amino acid amidation on peptidoglycan physiology, 3) understanding the function of unusual L,D-transpeptidase enzymes that may modify the cell wall, and 4) understanding the factors that contribute to antibiotic resistance in M. tuberculosis. These research activities include collaborations with Dr. Dean Crick and Dr. Sebabrata Mahapatra (Colorado State University, Fort Collins, CO).

2. We are also collaborating with Dr. Scott Butler in the Department of Microbiology and Immunology here at the UR to investigate the function of toxin-antitoxin genes in M. tuberculosis and their potential role in latency in tuberculosis.

Tularemia and Biodefense Research
1. Development of genetic tools for Francisella tularensis and investigations into tularemia pathogenesis. Our early work on Francisella was to develop genetic methods to manipulate Francisella tularensis, the causative agent of tularemia and a Category A Select Agent. This work focused on developing new plasmid vectors, selectable markers, a method of allelic exchange and transposon mutagenesis, single-copy chromosomal integration systems, and protein secretion reporter systems. Future plans include the development of projects to investigate Francisella cell wall physiology and the roles that cell envelope components have in the innate immune response and tularemia pathogenesis.

 
 Selected Publications
List All   |   Timeline
  1. Feiler C, Fisher AC, Boock JT, Marrichi MJ, Wright L, Schmidpeter PA, Blankenfeldt W, Pavelka M, Delisa MP. Directed Evolution of Mycobacterium tuberculosis ß-Lactamase Reveals Gatekeeper Residue That Regulates Antibiotic Resistance and Catalytic Efficiency. PLoS One. 2013; 8(9):e73123.
    View in: PubMed
  2. Sanders AN, Pavelka MS. Phenotypic analysis of Eschericia coli mutants lacking L,D-transpeptidases. Microbiology. 2013 Sep; 159(Pt 9):1842-52.
    View in: PubMed
  3. Slayden RA, Jackson M, Zucker J, Ramirez MV, Dawson CC, Crew R, Sampson NS, Thomas ST, Jamshidi N, Sisk P, Caspi R, Crick DC, McNeil MR, Pavelka MS, Niederweis M, Siroy A, Dona V, McFadden J, Boshoff H, Lew JM. Updating and curating metabolic pathways of TB. Tuberculosis (Edinb). 2013 Jan; 93(1):47-59.
    View in: PubMed
  4. Mahapatra S, Piechota C, Gil F, Ma Y, Huang H, Scherman MS, Jones V, Pavelka MS, Moniz-Pereira J, Pimentel M, McNeil MR, Crick DC. Mycobacteriophage Ms6 LysA: a Peptidoglycan Amidase and a Useful Analytical Tool. Appl Environ Microbiol. 2013 Feb; 79(3):768-73.
    View in: PubMed
  5. LoVullo ED, Miller CN, Pavelka MS, Kawula TH. TetR-based gene regulation systems for Francisella tularensis. Appl Environ Microbiol. 2012 Oct; 78(19):6883-9.
    View in: PubMed
  6. Patru MM, Pavelka MS. A role for the class A penicillin-binding protein PonA2 in the survival of Mycobacterium smegmatis under conditions of nonreplication. J Bacteriol. 2010 Jun; 192(12):3043-54.
    View in: PubMed
  7. LoVullo ED, Molins-Schneekloth CR, Schweizer HP, Pavelka MS. Single-copy chromosomal integration systems for Francisella tularensis. Microbiology. 2009 Apr; 155(Pt 4):1152-63.
    View in: PubMed
  8. LoVullo ED, Sherrill LA, Pavelka MS. Improved shuttle vectors for Francisella tularensis genetics. FEMS Microbiol Lett. 2009 Feb; 291(1):95-102.
    View in: PubMed
  9. Pavelka MS. Allelic exchange of unmarked mutations in Mycobacterium tuberculosis. Methods Mol Biol. 2008; 435:191-201.
    View in: PubMed
  10. McCann JR, McDonough JA, Pavelka MS, Braunstein M. Beta-lactamase can function as a reporter of bacterial protein export during Mycobacterium tuberculosis infection of host cells. Microbiology. 2007 Oct; 153(Pt 10):3350-9.
    View in: PubMed
  11. Kincaid EZ, Wolf AJ, Desvignes L, Mahapatra S, Crick DC, Brennan PJ, Pavelka MS, Ernst JD. Codominance of TLR2-dependent and TLR2-independent modulation of MHC class II in Mycobacterium tuberculosis infection in vivo. J Immunol. 2007 Sep 1; 179(5):3187-95.
    View in: PubMed
  12. Titball RW, Sjostedt A, Pavelka MS, Nano FE. Biosafety and selectable markers. Ann N Y Acad Sci. 2007 Jun; 1105:405-17.
    View in: PubMed
  13. Pavelka MS. Another brick in the wall. Trends Microbiol. 2007 Apr; 15(4):147-9.
    View in: PubMed
  14. LoVullo ED, Sherrill LA, Perez LL, Pavelka MS. Genetic tools for highly pathogenic Francisella tularensis subsp. tularensis. Microbiology. 2006 Nov; 152(Pt 11):3425-35.
    View in: PubMed
  15. McDonough JA, Hacker KE, Flores AR, Pavelka MS, Braunstein M. The twin-arginine translocation pathway of Mycobacterium smegmatis is functional and required for the export of mycobacterial beta-lactamases. J Bacteriol. 2005 Nov; 187(22):7667-79.
    View in: PubMed
  16. Flores AR, Parsons LM, Pavelka MS. Characterization of novel Mycobacterium tuberculosis and Mycobacterium smegmatis mutants hypersusceptible to beta-lactam antibiotics. J Bacteriol. 2005 Mar; 187(6):1892-900.
    View in: PubMed
  17. Consaul SA, Wright LF, Mahapatra S, Crick DC, Pavelka MS. An unusual mutation results in the replacement of diaminopimelate with lanthionine in the peptidoglycan of a mutant strain of Mycobacterium smegmatis. J Bacteriol. 2005 Mar; 187(5):1612-20.
    View in: PubMed
  18. Flores AR, Parsons LM, Pavelka MS. Genetic analysis of the beta-lactamases of Mycobacterium tuberculosis and Mycobacterium smegmatis and susceptibility to beta-lactam antibiotics. Microbiology. 2005 Feb; 151(Pt 2):521-32.
    View in: PubMed
  19. Raymond JB, Mahapatra S, Crick DC, Pavelka MS. Identification of the namH gene, encoding the hydroxylase responsible for the N-glycolylation of the mycobacterial peptidoglycan. J Biol Chem. 2005 Jan 7; 280(1):326-33.
    View in: PubMed
  20. Consaul SA, Pavelka MS. Use of a novel allele of the Escherichia coli aacC4 aminoglycoside resistance gene as a genetic marker in mycobacteria. FEMS Microbiol Lett. 2004 May 15; 234(2):297-301.
    View in: PubMed
  21. Raymond JB, Price NP, Pavelka MS. A method for the enzymatic synthesis and HPLC purification of the peptidoglycan precursor UDP-N-acetylmuramic acid. FEMS Microbiol Lett. 2003 Dec 5; 229(1):83-9.
    View in: PubMed
  22. Consaul SA, Jacobs WR, Pavelka MS. Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs of Mycobacterium smegmatis. FEMS Microbiol Lett. 2003 Aug 8; 225(1):131-5.
    View in: PubMed
  23. Pavelka MS, Chen B, Kelley CL, Collins FM, Jacobs Jr WR. Vaccine efficacy of a lysine auxotroph of Mycobacterium tuberculosis. Infect Immun. 2003 Jul; 71(7):4190-2.
    View in: PubMed
  24. Gokulan K, Rupp B, Pavelka MS, Jacobs WR, Sacchettini JC. Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis. J Biol Chem. 2003 May 16; 278(20):18588-96.
    View in: PubMed
  25. Cameron P, Bingham D, Paul A, Pavelka M, Cameron S, Rotondo D, Plevin R. Essential role for verotoxin in sustained stress-activated protein kinase and nuclear factor kappa B signaling, stimulated by Escherichia coli O157:H7 in Vero cells. Infect Immun. 2002 Oct; 70(10):5370-80.
    View in: PubMed
  26. Pavelka MS, Jacobs WR. Comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, Mycobacterium bovis bacillus Calmette-Guérin, and Mycobacterium tuberculosis H37Rv by allelic exchange. J Bacteriol. 1999 Aug; 181(16):4780-9.
    View in: PubMed
  27. Pavelka MS, Weisbrod TR, Jacobs WR. Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis. J Bacteriol. 1997 Apr; 179(8):2777-82.
    View in: PubMed
  28. Pavelka MS, Jacobs WR. Biosynthesis of diaminopimelate, the precursor of lysine and a component of peptidoglycan, is an essential function of Mycobacterium smegmatis. J Bacteriol. 1996 Nov; 178(22):6496-507.
    View in: PubMed
  29. Balasubramanian V, Pavelka MS, Bardarov SS, Martin J, Weisbrod TR, McAdam RA, Bloom BR, Jacobs WR. Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates. J Bacteriol. 1996 Jan; 178(1):273-9.
    View in: PubMed
  30. Pavelka MS, Hayes SF, Silver RP. Characterization of KpsT, the ATP-binding component of the ABC-transporter involved with the export of capsular polysialic acid in Escherichia coli K1. J Biol Chem. 1994 Aug 5; 269(31):20149-58.
    View in: PubMed
  31. Pavelka MS, Wright LF, Silver RP. Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1. J Bacteriol. 1991 Aug; 173(15):4603-10.
    View in: PubMed

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