Lynne Maquat
| Title | Professor |
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| Institution | School of Medicine and Dentistry |
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| Department | Biochemistry and Biophysics |
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| Address | University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 712
Rochester NY 14642
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| Title | Director, Center for RNA Biology |
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| Institution | University of Rochester Medical Center |
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| Department | Office of VP for Health Sciences |
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| Title | Professor |
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| Institution | University of Rochester Medical Center |
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| Department | Cancer Center |
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| 2012 |
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| Batsheva de Rothschild Fellow of the Israel Academy of Sciences and Humanities |
Research in my lab focuses on RNA decay pathways. One pathway, called nonsense-mediated mRNA decay (NMD) or mRNA surveillance, surveys all newly synthesized mRNAs during what we call a "pioneer" round of translation. This round of translation involves mRNA that is associated with the cap binding heterodimer CBP80 and CBP20. It is distinct from the type of translation that supports the bulk of cellular protein synthesis and involves a different cap binding protein, eukaryotic initiation factor (eIF) 4E. Generally, if translation terminates more than 50-55 nt upstream of an exon-exon junction that is marked by the NMD factors Upf3 or Upf3X, Upf2 and ultimately Upf1, then the mRNA will be subject to NMD. By the time CBP80 and CBP20 have been replaced by eIF4E, the Upf mark has been removed so that mRNA is immune to NMD.
Studies in progress will significantly advance our understanding of the mRNP proteins, translation factors and nucleases that trigger NMD. Our results will be useful when designing therapies that aim to abrogate NMD in order to abrogate the severity of nonsense-generated diseases. We are also interested in further characterizing the pioneer translation initiation complex and requirements for its remodeling to the steady-state initiation complex that involves eIF4E. Additionally, we are interested in the cycle of posttranslational modifications that typify at least some of the NMD factors, including phosphorylation of Upf1 that is mediated by the PI 3-kinase-related protein kinase Smg1.
We have also uncovered a new mRNA decay pathway that we call Staufen (Stau)1-mediated mRNA decay (SMD). This pathway provides cells with a previously unappreciated means to regulate gene expression posttranscriptionally. We have found that the double-stranded RNA binding protein Stau1 recruits the NMD factor Upf1 to mRNAs, a number of which have been identified using microarray analysis in collaboration with Luc DesGroseillers (Université de Montréal). For those mRNAs that we have studied in detail, Stau1 recruits Upf1 to the 3' UTR and elicits mRNA decay in way that depends on translation termination at the normal (i.e., upstream) termination codon. By so doing, Stau1 bypasses the need for the Upf3 or Upf3X and Upf2 NMD factors, which serve to recruit Upf1 during NMD. More recent microarray and other types of analyses of mRNAs that are upregulated when Stau1 is downregulated indicate that SMD is widely used by cells as a means of posttranscriptional control.
Please visit our lab website for more information.
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Park E, Gleghorn ML, Maquat LE. Staufen2 functions in Staufen1-mediated mRNA decay by binding to itself and its paralog and promoting UPF1 helicase but not ATPase activity. Proc Natl Acad Sci U S A. 2013 Jan 8; 110(2):405-12.
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Gong C, Popp MW, Maquat LE. Biochemical analysis of long non-coding RNA-containing ribonucleoprotein complexes. Methods. 2012 Oct; 58(2):88-93.
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Schoenberg DR, Maquat LE. Regulation of cytoplasmic mRNA decay. Nat Rev Genet. 2012; 13(4):246-59.
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Hwang J, Maquat LE. Nonsense-mediated mRNA decay (NMD) in animal embryogenesis: to die or not to die, that is the question. Curr Opin Genet Dev. 2011 Aug; 21(4):422-30.
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Gong C, Maquat LE. "Alu"strious long ncRNAs and their role in shortening mRNA half-lives. Cell Cycle. 2011 Jun 15; 10(12):1882-3.
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Ju S, Tardiff DF, Han H, Divya K, Zhong Q, Maquat LE, Bosco DA, Hayward LJ, Brown RH, Lindquist S, Ringe D, Petsko GA. A yeast model of FUS/TLS-dependent cytotoxicity. PLoS Biol. 2011 Apr; 9(4):e1001052.
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Maquat LE, Hwang J, Sato H, Tang Y. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harb Symp Quant Biol. 2010; 75:127-34.
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Gleghorn ML, Maquat LE. UPF1 learns to relax and unwind. Mol Cell. 2011 Mar 18; 41(6):621-3.
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Gong C, Maquat LE. lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3' UTRs via Alu elements. Nature. 2011 Feb 10; 470(7333):284-8.
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Maquat LE, Tarn WY, Isken O. The pioneer round of translation: features and functions. Cell. 2010 Aug 6; 142(3):368-74.
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Hwang J, Sato H, Tang Y, Matsuda D, Maquat LE. UPF1 association with the cap-binding protein, CBP80, promotes nonsense-mediated mRNA decay at two distinct steps. Mol Cell. 2010 Aug 13; 39(3):396-409.
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Maquat LE, Gong C. Gene expression networks: competing mRNA decay pathways in mammalian cells. Biochem Soc Trans. 2009 Dec; 37(Pt 6):1287-92.
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Isken O, Maquat LE. Telomeric RNAs as a novel player in telomeric integrity. F1000 Biol Rep. 2009; 1:90.
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Sato H, Maquat LE. Remodeling of the pioneer translation initiation complex involves translation and the karyopherin importin beta. Genes Dev. 2009 Nov 1; 23(21):2537-50.
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Schoenberg DR, Maquat LE. Re-capping the message. Trends Biochem Sci. 2009 Sep; 34(9):435-42.
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Gong C, Kim YK, Woeller CF, Tang Y, Maquat LE. SMD and NMD are competitive pathways that contribute to myogenesis: effects on PAX3 and myogenin mRNAs. Genes Dev. 2009 Jan 1; 23(1):54-66.
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Isken O, Maquat LE. The multiple lives of NMD factors: balancing roles in gene and genome regulation. Nat Rev Genet. 2008 Sep; 9(9):699-712.
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Saltzman AL, Kim YK, Pan Q, Fagnani MM, Maquat LE, Blencowe BJ. Regulation of multiple core spliceosomal proteins by alternative splicing-coupled nonsense-mediated mRNA decay. Mol Cell Biol. 2008 Jul; 28(13):4320-30.
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Isken O, Kim YK, Hosoda N, Mayeur GL, Hershey JW, Maquat LE. Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay. Cell. 2008 Apr 18; 133(2):314-27.
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Woeller CF, Gaspari M, Isken O, Maquat LE. NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA. EMBO Rep. 2008 May; 9(5):446-51.
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Sato H, Hosoda N, Maquat LE. Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay. Mol Cell. 2008 Feb 1; 29(2):255-62.
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Maquat LE, Kiledjian M. RNA turnover in eukaryotes: nucleases, pathways and analysis of mRNA decay. Preface. Methods Enzymol. 2008; 448:xxi-xxii.
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Matsuda D, Sato H, Maquat LE. Chapter 9. Studying nonsense-mediated mRNA decay in mammalian cells. Methods Enzymol. 2008; 449:177-201.
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Maquat LE, Kiledjian M. RNA turnover in eukaryotes: analysis of specialized and quality control RNA decay pathways. Preface. Methods Enzymol. 2008; 449:xvii-xviii.
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Maquat LE, Arraiano CM. RNA turnover in prokaryotes, archaea and organelles. Preface. Methods Enzymol. 2008; 447:xxiii-xxiv.
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Matsuda D, Hosoda N, Kim YK, Maquat LE. Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA. Nat Struct Mol Biol. 2007 Oct; 14(10):974-9.
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Isken O, Maquat LE. Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function. Genes Dev. 2007 Aug 1; 21(15):1833-56.
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Kim YK, Furic L, Parisien M, Major F, DesGroseillers L, Maquat LE. Staufen1 regulates diverse classes of mammalian transcripts. EMBO J. 2007 Jun 6; 26(11):2670-81.
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Kuzmiak HA, Maquat LE. Applying nonsense-mediated mRNA decay research to the clinic: progress and challenges. Trends Mol Med. 2006 Jul; 12(7):306-16.
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Hosoda N, Lejeune F, Maquat LE. Evidence that poly(A) binding protein C1 binds nuclear pre-mRNA poly(A) tails. Mol Cell Biol. 2006 Apr; 26(8):3085-97.
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Pan Q, Saltzman AL, Kim YK, Misquitta C, Shai O, Maquat LE, Frey BJ, Blencowe BJ. Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression. Genes Dev. 2006 Jan 15; 20(2):153-8.
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Hosoda N, Kim YK, Lejeune F, Maquat LE. CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells. Nat Struct Mol Biol. 2005 Oct; 12(10):893-901.
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Lejeune F, Maquat LE. Mechanistic links between nonsense-mediated mRNA decay and pre-mRNA splicing in mammalian cells. Curr Opin Cell Biol. 2005 Jun; 17(3):309-15.
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Maquat LE. Nonsense-mediated mRNA decay in mammals. J Cell Sci. 2005 May 1; 118(Pt 9):1773-6.
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Gao Q, Das B, Sherman F, Maquat LE. Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast. Proc Natl Acad Sci U S A. 2005 Mar 22; 102(12):4258-63.
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Kim YK, Furic L, Desgroseillers L, Maquat LE. Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so as to elicit mRNA decay. Cell. 2005 Jan 28; 120(2):195-208.
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Valencia-Sánchez MA, Maquat LE. An enemy within: fly reconnaissance deploys an endonuclease to destroy nonsense-containing mRNA. Trends Cell Biol. 2004 Nov; 14(11):594-7.
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Lejeune F, Ranganathan AC, Maquat LE. eIF4G is required for the pioneer round of translation in mammalian cells. Nat Struct Mol Biol. 2004 Oct; 11(10):992-1000.
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Brumbaugh KM, Otterness DM, Geisen C, Oliveira V, Brognard J, Li X, Lejeune F, Tibbetts RS, Maquat LE, Abraham RT. The mRNA surveillance protein hSMG-1 functions in genotoxic stress response pathways in mammalian cells. Mol Cell. 2004 Jun 4; 14(5):585-98.
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Chiu SY, Lejeune F, Ranganathan AC, Maquat LE. The pioneer translation initiation complex is functionally distinct from but structurally overlaps with the steady-state translation initiation complex. Genes Dev. 2004 Apr 1; 18(7):745-54.
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Maquat LE. Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics. Nat Rev Mol Cell Biol. 2004 Feb; 5(2):89-99.
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Lejeune F, Maquat LE. Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells. Methods Mol Biol. 2004; 257:115-24.
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Lejeune F, Li X, Maquat LE. Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities. Mol Cell. 2003 Sep; 12(3):675-87.
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Arraiano CM, Maquat LE. Post-transcriptional control of gene expression: effectors of mRNA decay. Mol Microbiol. 2003 Jul; 49(1):267-76.
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Chiu SY, Serin G, Ohara O, Maquat LE. Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1. RNA. 2003 Jan; 9(1):77-87.
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Stevens A, Wang Y, Bremer K, Zhang J, Hoepfner R, Antoniou M, Schoenberg DR, Maquat LE. Beta -Globin mRNA decay in erythroid cells: UG site-preferred endonucleolytic cleavage that is augmented by a premature termination codon. Proc Natl Acad Sci U S A. 2002 Oct 1; 99(20):12741-6.
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Maquat LE. NASty effects on fibrillin pre-mRNA splicing: another case of ESE does it, but proposals for translation-dependent splice site choice live on. Genes Dev. 2002 Jul 15; 16(14):1743-53.
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Lejeune F, Ishigaki Y, Li X, Maquat LE. The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling. EMBO J. 2002 Jul 1; 21(13):3536-45.
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Maquat LE. Molecular biology. Skiing toward nonstop mRNA decay. Science. 2002 Mar 22; 295(5563):2221-2.
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Maquat LE. Nonsense-mediated mRNA decay. Curr Biol. 2002 Mar 19; 12(6):R196-7.
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Maquat LE. RNA-protein interactions: insight into gene function. Methods. 2002 Feb; 26(2):93-4.
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Sun X, Maquat LE. Nonsense-mediated decay: assaying for effects on selenoprotein mRNAs. Methods Enzymol. 2002; 347:49-57.
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Schneider R, Agol VI, Andino R, Bayard F, Cavener DR, Chappell SA, Chen JJ, Darlix JL, Dasgupta A, Donzé O, Duncan R, Elroy-Stein O, Farabaugh PJ, Filipowicz W, Gale M, Gehrke L, Goldman E, Groner Y, Harford JB, Hatzglou M, He B, Hellen CU, Hentze MW, Hershey J, Hershey P, Hohn T, Holcik M, Hunter CP, Igarashi K, Jackson R, Jagus R, Jefferson LS, Joshi B, Kaempfer R, Katze M, Kaufman RJ, Kiledjian M, Kimball SR, Kimchi A, Kirkegaard K, Koromilas AE, Krug RM, Kruys V, Lamphear BJ, Lemon S, Lloyd RE, Maquat LE, Martinez-Salas E, Mathews MB, Mauro VP, Miyamoto S, Mohr I, Morris DR, Moss EG, Nakashima N, Palmenberg A, Parkin NT, Pe'ery T, Pelletier J, Peltz S, Pestova TV, Pilipenko EV, Prats AC, Racaniello V, Read GS, Rhoads RE, Richter JD, Rivera-Pomar R, Rouault T, Sachs A, Sarnow P, Scheper GC, Schiff L, Schoenberg DR, Semler BL, Siddiqui A, Skern T, Sonenberg N, Sossin W, Standart N, Tahara SM, Thomas AA, Toulmé JJ, Wilusz J, Wimmer E, Witherell G, Wormington M. New ways of initiating translation in eukaryotes. Mol Cell Biol. 2001 Dec; 21(23):8238-46.
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Ishigaki Y, Li X, Serin G, Maquat LE. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20. Cell. 2001 Sep 7; 106(5):607-17.
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Denning G, Jamieson L, Maquat LE, Thompson EA, Fields AP. Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein. J Biol Chem. 2001 Jun 22; 276(25):22709-14.
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Sun X, Li X, Moriarty PM, Henics T, LaDuca JP, Maquat LE. Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked or inhibited in rat tissues. Mol Biol Cell. 2001 Apr; 12(4):1009-17.
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Maquat LE, Li X. Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay. RNA. 2001 Mar; 7(3):445-56.
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Maquat LE, Carmichael GG. Quality control of mRNA function. Cell. 2001 Jan 26; 104(2):173-6.
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Maquat LE. Evidence that selenium deficiency results in the cytoplasmic decay of GPx1 mRNA dependent on pre-mRNA splicing proteins bound to the mRNA exon-exon junction. Biofactors. 2001; 14(1-4):37-42.
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Serin G, Gersappe A, Black JD, Aronoff R, Maquat LE. Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4). Mol Cell Biol. 2001 Jan; 21(1):209-23.
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Maquat LE, Serin G. Nonsense-mediated mRNA decay: insights into mechanism from the cellular abundance of human Upf1, Upf2, Upf3, and Upf3X proteins. Cold Spring Harb Symp Quant Biol. 2001; 66:313-20.
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Maquat LE. The power of point mutations. Nat Genet. 2001 Jan; 27(1):5-6.
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Pal M, Ishigaki Y, Nagy E, Maquat LE. Evidence that phosphorylation of human Upfl protein varies with intracellular location and is mediated by a wortmannin-sensitive and rapamycin-sensitive PI 3-kinase-related kinase signaling pathway. RNA. 2001 Jan; 7(1):5-15.
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Le Hir H, Izaurralde E, Maquat LE, Moore MJ. The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. EMBO J. 2000 Dec 15; 19(24):6860-9.
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Sun X, Moriarty PM, Maquat LE. Nonsense-mediated decay of glutathione peroxidase 1 mRNA in the cytoplasm depends on intron position. EMBO J. 2000 Sep 1; 19(17):4734-44.
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Le Hir H, Moore MJ, Maquat LE. Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions. Genes Dev. 2000 May 1; 14(9):1098-108.
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Valentin C, Cohen-Solal M, Maquat L, Horányi M, Inselt-Kovács M, Hollán S. Identical germ-line mutations in the triosephosphate isomerase alleles of two brothers are associated with distinct clinical phenotypes. C R Acad Sci III. 2000 Mar; 323(3):245-50.
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Sun X, Maquat LE. mRNA surveillance in mammalian cells: the relationship between introns and translation termination. RNA. 2000 Jan; 6(1):1-8.
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Zhang J, Sun X, Qian Y, LaDuca JP, Maquat LE. At least one intron is required for the nonsense-mediated decay of triosephosphate isomerase mRNA: a possible link between nuclear splicing and cytoplasmic translation. Mol Cell Biol. 1998 Sep; 18(9):5272-83.
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Sun X, Perlick HA, Dietz HC, Maquat LE. A mutated human homologue to yeast Upf1 protein has a dominant-negative effect on the decay of nonsense-containing mRNAs in mammalian cells. Proc Natl Acad Sci U S A. 1998 Aug 18; 95(17):10009-14.
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Zhang J, Sun X, Qian Y, Maquat LE. Intron function in the nonsense-mediated decay of beta-globin mRNA: indications that pre-mRNA splicing in the nucleus can influence mRNA translation in the cytoplasm. RNA. 1998 Jul; 4(7):801-15.
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Nagy E, Maquat LE. A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance. Trends Biochem Sci. 1998 Jun; 23(6):198-9.
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Moriarty PM, Reddy CC, Maquat LE. Selenium deficiency reduces the abundance of mRNA for Se-dependent glutathione peroxidase 1 by a UGA-dependent mechanism likely to be nonsense codon-mediated decay of cytoplasmic mRNA. Mol Cell Biol. 1998 May; 18(5):2932-9.
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Moriarty PM, Reddy CC, Maquat LE. The presence of an intron within the rat gene for selenium-dependent glutathione peroxidase 1 is not required to protect nuclear RNA from UGA-mediated decay. RNA. 1997 Dec; 3(12):1369-73.
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Zhang J, Maquat LE. Evidence that translation reinitiation abrogates nonsense-mediated mRNA decay in mammalian cells. EMBO J. 1997 Feb 17; 16(4):826-33.
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Maquat LE. Defects in RNA splicing and the consequence of shortened translational reading frames. Am J Hum Genet. 1996 Aug; 59(2):279-86.
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Zhang J, Maquat LE. Evidence that the decay of nucleus-associated nonsense mRNA for human triosephosphate isomerase involves nonsense codon recognition after splicing. RNA. 1996 Mar; 2(3):235-43.
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Stephenson LS, Maquat LE. Cytoplasmic mRNA for human triosephosphate isomerase is immune to nonsense-mediated decay despite forming polysomes. Biochimie. 1996; 78(11-12):1043-7.
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Maquat LE. When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells. RNA. 1995 Jul; 1(5):453-65.
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Nesic D, Zhang J, Maquat LE. Lack of an effect of the efficiency of RNA 3'-end formation on the efficiency of removal of either the final or the penultimate intron in intact cells. Mol Cell Biol. 1995 Jan; 15(1):488-96.
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Belgrader P, Cheng J, Zhou X, Stephenson LS, Maquat LE. Mammalian nonsense codons can be cis effectors of nuclear mRNA half-life. Mol Cell Biol. 1994 Dec; 14(12):8219-28.
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Belgrader P, Maquat LE. Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping. Mol Cell Biol. 1994 Sep; 14(9):6326-36.
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Cheng J, Belgrader P, Zhou X, Maquat LE. Introns are cis effectors of the nonsense-codon-mediated reduction in nuclear mRNA abundance. Mol Cell Biol. 1994 Sep; 14(9):6317-25.
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Nesic D, Maquat LE. Upstream introns influence the efficiency of final intron removal and RNA 3'-end formation. Genes Dev. 1994 Feb 1; 8(3):363-75.
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Chang ML, Artymiuk PJ, Wu X, Hollán S, Lammi A, Maquat LE. Human triosephosphate isomerase deficiency resulting from mutation of Phe-240. Am J Hum Genet. 1993 Jun; 52(6):1260-9.
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Nesic D, Cheng J, Maquat LE. Sequences within the last intron function in RNA 3'-end formation in cultured cells. Mol Cell Biol. 1993 Jun; 13(6):3359-69.
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Cheng J, Maquat LE. Nonsense codons can reduce the abundance of nuclear mRNA without affecting the abundance of pre-mRNA or the half-life of cytoplasmic mRNA. Mol Cell Biol. 1993 Mar; 13(3):1892-902.
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Belgrader P, Cheng J, Maquat LE. Evidence to implicate translation by ribosomes in the mechanism by which nonsense codons reduce the nuclear level of human triosephosphate isomerase mRNA. Proc Natl Acad Sci U S A. 1993 Jan 15; 90(2):482-6.
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Lim SK, Maquat LE. Human beta-globin mRNAs that harbor a nonsense codon are degraded in murine erythroid tissues to intermediates lacking regions of exon I or exons I and II that have a cap-like structure at the 5' termini. EMBO J. 1992 Sep; 11(9):3271-8.
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Lim SK, Sigmund CD, Gross KW, Maquat LE. Nonsense codons in human beta-globin mRNA result in the production of mRNA degradation products. Mol Cell Biol. 1992 Mar; 12(3):1149-61.
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Maquat LE. Nuclear mRNA export. Curr Opin Cell Biol. 1991 Dec; 3(6):1004-12.
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Boyer TG, Maquat LE. Modulation of human triosephosphate isomerase gene transcription by serum. J Biol Chem. 1991 Jul 15; 266(20):13350-4.
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Boyer TG, Maquat LE. Minimal sequence and factor requirements for the initiation of transcription from an atypical, TATATAA box-containing housekeeping promoter. J Biol Chem. 1990 Nov 25; 265(33):20524-32.
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Cheng J, Fogel-Petrovic M, Maquat LE. Translation to near the distal end of the penultimate exon is required for normal levels of spliced triosephosphate isomerase mRNA. Mol Cell Biol. 1990 Oct; 10(10):5215-25.
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Cheng J, Mielnicki LM, Pruitt SC, Maquat LE. Nucleotide sequence of murine triosephosphate isomerase cDNA. Nucleic Acids Res. 1990 Jul 25; 18(14):4261.
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Lim S, Mullins JJ, Chen CM, Gross KW, Maquat LE. Novel metabolism of several beta zero-thalassemic beta-globin mRNAs in the erythroid tissues of transgenic mice. EMBO J. 1989 Sep; 8(9):2613-9.
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Boyer TG, Krug JR, Maquat LE. Transcriptional regulatory sequences of the housekeeping gene for human triosephosphate isomerase. J Biol Chem. 1989 Mar 25; 264(9):5177-87.
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Daar IO, Maquat LE. Premature translation termination mediates triosephosphate isomerase mRNA degradation. Mol Cell Biol. 1988 Feb; 8(2):802-13.
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Daar IO, Artymiuk PJ, Phillips DC, Maquat LE. Human triose-phosphate isomerase deficiency: a single amino acid substitution results in a thermolabile enzyme. Proc Natl Acad Sci U S A. 1986 Oct; 83(20):7903-7.
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Baumann H, Maquat LE. Localization of DNA sequences involved in dexamethasone-dependent expression of the rat alpha 1-acid glycoprotein gene. Mol Cell Biol. 1986 Jul; 6(7):2551-61.
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Brown JR, Daar IO, Krug JR, Maquat LE. Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family. Mol Cell Biol. 1985 Jul; 5(7):1694-706.
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Maquat LE, Kinniburgh AJ. A beta zero-thalassemic beta-globin RNA that is labile in bone marrow cells is relatively stable in HeLa cells. Nucleic Acids Res. 1985 Apr 25; 13(8):2855-67.
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Maquat LE, Chilcote R, Ryan PM. Human triosephosphate isomerase cDNA and protein structure. Studies of triosephosphate isomerase deficiency in man. J Biol Chem. 1985 Mar 25; 260(6):3748-53.
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Kinniburgh AJ, Maquat LE, Schedl T, Rachmilewitz E, Ross J. mRNA-deficient beta o-thalassemia results from a single nucleotide deletion. Nucleic Acids Res. 1982 Sep 25; 10(18):5421-7.
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Maquat LE, Kinniburgh AJ, Rachmilewitz EA, Ross J. Unstable beta-globin mRNA in mRNA-deficient beta o thalassemia. Cell. 1981 Dec; 27(3 Pt 2):543-53.
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Maquat LE, Kinniburgh AJ, Beach LR, Honig GR, Lazerson J, Ershler WB, Ross J. Processing of human beta-globin mRNA precursor to mRNA is defective in three patients with beta+-thalassemia. Proc Natl Acad Sci U S A. 1980 Jul; 77(7):4287-91.
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Maquat LE, Thornton K, Reznikoff WS. lac Promoter mutations located downstream from the transcription start site. J Mol Biol. 1980 May 25; 139(3):537-49.
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Maquat LE, Reznikoff WS. lac Promoter mutation Pr115 generates a new transcription initiation point. J Mol Biol. 1980 May 25; 139(3):551-6.
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Patient RK, Hardies SC, Larson JE, Inman RB, Maquat LE, Wells RD. Influence of A-T content on the fractionation of DNA restriction fragments by RPC-5 column chromatography. J Biol Chem. 1979 Jun 25; 254(12):5548-54.
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Maquat LE, Reznikoff WS. In vitro analysis of the Escherichia coli RNA polymerase interaction with wild-type and mutant lactose promoters. J Mol Biol. 1978 Nov 15; 125(4):467-90.
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