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Lisa DeLouise

TitleAssociate Professor
InstitutionSchool of Medicine and Dentistry
DepartmentDermatology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 697
Rochester NY 14642
Other Positions
TitleAssociate Professor
InstitutionSchool of Medicine and Dentistry
DepartmentBiomedical Engineering

TitleAssociate Professor
InstitutionUniversity of Rochester, River Campus
DepartmentElectrical & Computer Engineering

 
 Overview
Research Interest:
The overall research area in the laboratory is the creation of Smart Bandage Biomaterial Engineering and Skin Disease. This laboratory investigates the fundamental optical, morphological, and surface chemical properties of bioengineered nanoporous silicon (PSi) in developing a platform of Smart Bandage technologies targeting biosensors for point of care diagnosis of cutaneous disease, transdermal drug delivery, and tissue engineering for wound healing. PSi is fabricated from single crystal silicon wafers using an electrochemical etch process. The pore diameter, porosity (surface area and internal void volume), bioerosion, and interferometric optical properties can be tailored over a wide range to suit the application. Ongoing projects involve developing a refractive index sensitive biosensor for detection of Candida ablicans for which we've developed methods to site direct the immobilization of phage display scFv antibody receptors. Tests are underway to evaluate detection sensitivity relative to nonspecifically immobilized whole IgG aCandida antibody receptor employing commercial antigen. Fundamental insights into the factors (pore size, steric crowding, operating frequency, device architecture, blocking agent, operation protocol, etc.) that impact detection sensitivity are being explored. Biosensor tests are typically conducted on devices attached to the silicon wafer. Recently, we developed methods to detach the sensor and mount it in a polymer support. We are conducting studies to characterize the diffusion characteristics of small molecules though hydrogel films (40 micron) cast over porous silicon sensor (4 um) by monitoring changes in the optical response that result when molecules diffuse in or out of the porous silicon layer. This work will enable us to develop models for designing transdermal drug delivery systems where the optical response can be measured, while applied to the skin, to monitor the time released delivery of therapeutics concentrated within the porous reservoir. We are interested in developing a smart bandage to deliver antifungal locally to the nail matrix where nail progenitor cells live. We are also investing the morphology and phenotype dermal human fibroblast and immortalized keratinocytes (HaCaT) cells cultured on chemically modified PSi and flat silicon wafer surfaces. The goal is systematically engineer the surface chemistry, energy and topography of biomaterial scaffolds to mimic the fetal reepithelialization process. Our hypothesis is that by controlling differential cell proliferation (keratinocyte vs. fibroblast) and cell phenotype, such as integrin and metalloproteinase expression profiles or the magnitude of actin fiber extensions, novel therapies will result that can accelerate the healing of chronic wounds (ulcers) and burns with reduced scarring. A unique aspect of this research program is the melding of cross-disciplinary skills in surface science, physical chemistry, microfluidic device engineering, optics, and biological and medical sciences. This interdisciplinary approach provides a firm basis for the investigation and fabrication of new biomedical devices, while enabling a broader perspective on quantifying biological efficacy and establishing clinical utility.

 
 Selected Publications
List All   |   Timeline
  1. Giang UB, Jones MC, Kaule MJ, Virgile CR, Pu Q, Delouise LA. Quantitative analysis of spherical microbubble cavity array formation in thermally cured polydimethylsiloxane for use in cell sorting applications. Biomed Microdevices. 2014 Feb; 16(1):55-67.
    View in: PubMed
  2. Jatana S, Delouise LA. Understanding engineered nanomaterial skin interactions and the modulatory effects of ultraviolet radiation skin exposure. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014 Jan; 6(1):61-79.
    View in: PubMed
  3. Jones MC, Kobie JJ, Delouise LA. Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomed Microdevices. 2013 Jun; 15(3):453-63.
    View in: PubMed
  4. Mortensen LJ, Jatana S, Gelein R, De Benedetto A, De Mesy Bentley KL, Beck LA, Elder A, Delouise LA. Quantification of quantum dot murine skin penetration with UVR barrier impairment. Nanotoxicology. 2013 Dec; 7:1386-98.
    View in: PubMed
  5. Zheng H, Mortensen LJ, DeLouise LA. Thiol antioxidant-functionalized CdSe/ZnS quantum dots: synthesis, characterization, cytotoxicity. J Biomed Nanotechnol. 2013 Mar; 9(3):382-92.
    View in: PubMed
  6. Mortensen LJ, Ravichandran S, Delouise LA. The impact of UVB exposure and differentiation state of primary keratinocytes on their interaction with quantum dots. Nanotoxicology. 2013 Nov; 7:1244-54.
    View in: PubMed
  7. Chandrasekaran S, Geng Y, DeLouise LA, King MR. Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines. Biomaterials. 2012 Dec; 33(35):9037-48.
    View in: PubMed
  8. DeLouise LA. Applications of nanotechnology in dermatology. J Invest Dermatol. 2012 Mar; 132(3 Pt 2):964-75.
    View in: PubMed
  9. Zheng H, Chen G, Song F, DeLouise LA, Lou Z. The cytotoxicity of OPA-modified CdSe/ZnS core/shell quantum dots and its modulation by silibinin in human skin cells. J Biomed Nanotechnol. 2011 Oct; 7(5):648-58.
    View in: PubMed
  10. Chandrasekaran S, DeLouise LA. Enriching and characterizing cancer stem cell sub-populations in the WM115 melanoma cell line. Biomaterials. 2011 Dec; 32(35):9316-27.
    View in: PubMed
  11. Chandrasekaran S, Giang UB, King MR, DeLouise LA. Microenvironment induced spheroid to sheeting transition of immortalized human keratinocytes (HaCaT) cultured in microbubbles formed in polydimethylsiloxane. Biomaterials. 2011 Oct; 32(29):7159-68.
    View in: PubMed
  12. Agastin S, Giang UB, Geng Y, Delouise LA, King MR. Continuously perfused microbubble array for 3D tumor spheroid model. Biomicrofluidics. 2011 Jun; 5(2):24110.
    View in: PubMed
  13. Mortensen LJ, Glazowski CE, Zavislan JM, Delouise LA. Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin. Biomed Opt Express. 2011 Jun 1; 2(6):1610-25.
    View in: PubMed
  14. Ravichandran S, Mortensen LJ, DeLuise LA. Quantification of human skin barrier function and susceptibility to quantum dot skin penetration. Nanotoxicology. 2011 Dec; 5(4):675-86.
    View in: PubMed
  15. Bonanno LM, Kwong TC, DeLouise LA. Label-free porous silicon immunosensor for broad detection of opiates in a blind clinical study and results comparison to commercial analytical chemistry techniques. Anal Chem. 2010 Dec 1; 82(23):9711-8.
    View in: PubMed
  16. Mortensen LJ, Ravichandran S, Zheng H, DeLouise LA. Progress and challenges in quantifying skin permeability to nanoparticles using a quantum dot model. J Biomed Nanotechnol. 2010 Oct; 6(5):596-604.
    View in: PubMed
  17. Zheng H, Chen G, DeLouise LA, Lou Z. Detection of the cancer marker CD146 expression in melanoma cells with semiconductor quantum dot label. J Biomed Nanotechnol. 2010 Aug; 6(4):303-11.
    View in: PubMed
  18. Bonanno LM, Delouise LA. Tunable detection sensitivity of opiates in urine via a label-free porous silicon competitive inhibition immunosensor. Anal Chem. 2010 Jan 15; 82(2):714-22.
    View in: PubMed
  19. Elder A, Vidyasagar S, DeLouise L. Physicochemical factors that affect metal and metal oxide nanoparticle passage across epithelial barriers. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2009 Jul-Aug; 1(4):434-50.
    View in: PubMed
  20. Mortensen LJ, Oberdörster G, Pentland AP, Delouise LA. In vivo skin penetration of quantum dot nanoparticles in the murine model: the effect of UVR. Nano Lett. 2008 Sep; 8(9):2779-87.
    View in: PubMed
  21. Furbert P, Lu C, Winograd N, DeLouise L. Label-free optical detection of peptide synthesis on a porous silicon scaffold/sensor. Langmuir. 2008 Mar 18; 24(6):2908-15.
    View in: PubMed
  22. Giang UB, Lee D, King MR, DeLouise LA. Microfabrication of cavities in polydimethylsiloxane using DRIE silicon molds. Lab Chip. 2007 Dec; 7(12):1660-2.
    View in: PubMed
  23. Rabus DG, DeLouise LA, Ichihashi Y. Enhancement of the evanescent field using polymer waveguides fabricated by deep UV exposure on mesoporous silicon. Opt Lett. 2007 Oct 1; 32(19):2843-5.
    View in: PubMed
  24. Bonanno LM, DeLouise LA. Whole blood optical biosensor. Biosens Bioelectron. 2007 Oct 31; 23(3):444-8.
    View in: PubMed
  25. Bonanno LM, Delouise LA. Steric crowding effects on target detection in an affinity biosensor. Langmuir. 2007 May 8; 23(10):5817-23.
    View in: PubMed
  26. Ouyang H, Delouise LA, Miller BL, Fauchet PM. Label-free quantitative detection of protein using macroporous silicon photonic bandgap biosensors. Anal Chem. 2007 Feb 15; 79(4):1502-6.
    View in: PubMed
  27. DeLouise LA, Kou PM, Miller BL. Cross-correlation of optical microcavity biosensor response with immobilized enzyme activity. Insights into biosensor sensitivity. Anal Chem. 2005 May 15; 77(10):3222-30.
    View in: PubMed
  28. Delouise LA, Miller BL. Enzyme immobilization in porous silicon: quantitative analysis of the kinetic parameters for glutathione-S-transferases. Anal Chem. 2005 Apr 1; 77(7):1950-6.
    View in: PubMed
  29. DeLouise LA, Miller BL. Quantatitive assessment of enzyme immobilization capacity in porous silicon. Anal Chem. 2004 Dec 1; 76(23):6915-20.
    View in: PubMed

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