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Peter Sims

TitleProfessor Emeritus
InstitutionSchool of Medicine and Dentistry
DepartmentMedicine
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box MED
Rochester NY 14642
 
 Awards And Honors
1972     Sigma Xi, Amherst College
1972     Phi Beta Kappa, Amherst College
1974     B.A., Summa Cum Laude, Amherst College
1974 - 1980Medical Scientist Traineeship (PHS), GM-07171
1976     Alpha Omega Alpha, Duke University School of Medicine
1981     Southern Medical Association Research Award
1982     Clinician-Scientist Award, American Heart Association (Awarded but not activated in lieu of Hartford Fellowship)
1982 - 1985John A. and George L. Hartford Fellowship in Biomedical Research
1985     N.I.H. Research Career Development Award (Awarded but not activated in lieu of AHA Estabished Investigatorship)
1985 - 1990Established Investigator of American Heart Association #85-128
1988     American Society for Clinical Investigation
1988 - 1992S. Graham Smith Endowment Distinguished Scientist, Oklahoma Medical Research Foundation,
1989     Man-of-the-Year Award, American Heart Association, Oklahoma Affiliate Chapter
1990 - 1991President, American Heart Association, Oklahoma Affiliate
1991 - 1992Chairman, Veterans Administration Merit Review Board - Hematology
1993 - 1999Walter H. Schroeder Chair in Research, The Blood Center of Southeastern Wisconsin
1995     N.I.H. (NHLBI) MERIT Award
1999 - 2001Chairman, N.I.H. Hematology 1 Study Section
2004     Adjunct Professor Shanghai Second Medical University Shanghai, China
 
 Overview
We originally discovered and cloned phospholipid scramblase as a membrane protein with capacity to promote rapid transbilayer movement of phospholipids in response to Ca2+ . This protein turned out to be the first of four that together form the phospholipid scramblase (PLSCR) gene family. Our research during the last few years has revealed that PLSCRs may have functions beyond their putative role as membrane phospholipid scramblases. Taking advantage of gene knockout technology, we discovered that mice with a targeted deletion of PLSCR1 display perinatal granulocytopenia due to defective response to hematologic precursors to granulocyte colony-stimulating factor and stem cell factor. Interestingly, PLSCR3-/- mice have a phenotype reminiscent of metabolic syndrome in man: they display aberrant accumulation of abdominal fat, accompanied by the development of insulin resistance, glucose intolerance, and dyslipidemia. These observations and our in vitro data suggest that PLSCRs are involved in signal transduction through growth factor receptors and may be required for normal cell maturation. Furthermore, we have shown that aberrant expression of PLSCR1 may play a role in tumor formation and increased susceptibility to viral infection. Current efforts are focused on unraveling the molecular mechanisms by which PLSCRs mediate these diverse functions.

 
 Selected Publications
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  1. Kodigepalli KM, Anur P, Spellman P, Sims PJ, Nanjundan M. Phospholipid Scramblase 1, an interferon-regulated gene located at 3q23, is regulated by SnoN/SkiL in ovarian cancer cells. Mol Cancer. 2013; 12(1):32.
    View in: PubMed
  2. Jackson BR, Krasowski MD, Sims PJ. Laboratory diagnostic oversight committees and the profession of laboratory medicine. Am J Clin Pathol. 2013 Mar; 139(3):273-4.
    View in: PubMed
  3. Ory S, Ceridono M, Momboisse F, Houy S, Chasserot-Golaz S, Heintz D, Calco V, Haeberlé AM, Espinoza FA, Sims PJ, Bailly Y, Bader MF, Gasman S. Phospholipid scramblase-1-induced lipid reorganization regulates compensatory endocytosis in neuroendocrine cells. J Neurosci. 2013 Feb 20; 33(8):3545-56.
    View in: PubMed
  4. Li D, Yang H, Nan H, Liu P, Pang S, Zhao Q, Karni R, Kamps MP, Xu Y, Zhou J, Wiedmer T, Sims PJ, Wang F. Identification of key regulatory pathways of myeloid differentiation using an mESC-based karyotypically normal cell model. Blood. 2012 Dec 6; 120(24):4712-9.
    View in: PubMed
  5. Kirov A, Al-Hashimi H, Solomon P, Mazur C, Thorpe PE, Sims PJ, Tarantini F, Kumar TK, Prudovsky I. Phosphatidylserine externalization and membrane blebbing are involved in the nonclassical export of FGF1. J Cell Biochem. 2012 Mar; 113(3):956-66.
    View in: PubMed
  6. Lott K, Bhardwaj A, Sims PJ, Cingolani G. A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1. J Biol Chem. 2011 Aug 12; 286(32):28160-9.
    View in: PubMed
  7. Chen CW, Sowden M, Zhao Q, Wiedmer T, Sims PJ. Nuclear phospholipid scramblase 1 prolongs the mitotic expansion of granulocyte precursors during G-CSF-induced granulopoiesis. J Leukoc Biol. 2011 Aug; 90(2):221-33.
    View in: PubMed
  8. Suzuki J, Umeda M, Sims PJ, Nagata S. Calcium-dependent phospholipid scrambling by TMEM16F. Nature. 2010 Dec 9; 468(7325):834-8.
    View in: PubMed
  9. Bateman A, Finn RD, Sims PJ, Wiedmer T, Biegert A, Söding J. Phospholipid scramblases and Tubby-like proteins belong to a new superfamily of membrane tethered transcription factors. Bioinformatics. 2009 Jan 15; 25(2):159-62.
    View in: PubMed
  10. Lu B, Sims PJ, Wiedmer T, Moser AH, Shigenaga JK, Grunfeld C, Feingold KR. Expression of the phospholipid scramblase (PLSCR) gene family during the acute phase response. Biochim Biophys Acta. 2007 Sep; 1771(9):1177-85.
    View in: PubMed
  11. Mutch DM, O'Maille G, Wikoff WR, Wiedmer T, Sims PJ, Siuzdak G. Mobilization of pro-inflammatory lipids in obese Plscr3-deficient mice. Genome Biol. 2007; 8(3):R38.
    View in: PubMed
  12. Huang Y, Zhao Q, Zhou CX, Gu ZM, Li D, Xu HZ, Wiedmer T, Sims PJ, Zhao KW, Chen GQ. Antileukemic roles of human phospholipid scramblase 1 gene, evidence from inducible PLSCR1-expressing leukemic cells. Oncogene. 2006 Oct 26; 25(50):6618-27.
    View in: PubMed
  13. Li Y, Rogulski K, Zhou Q, Sims PJ, Prochownik EV. The negative c-Myc target onzin affects proliferation and apoptosis via its obligate interaction with phospholipid scramblase 1. Mol Cell Biol. 2006 May; 26(9):3401-13.
    View in: PubMed
  14. Zhao KW, Li D, Zhao Q, Huang Y, Silverman RH, Sims PJ, Chen GQ. Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK. J Biol Chem. 2005 Dec 30; 280(52):42707-14.
    View in: PubMed
  15. Zhou Q, Ben-Efraim I, Bigcas JL, Junqueira D, Wiedmer T, Sims PJ. Phospholipid scramblase 1 binds to the promoter region of the inositol 1,4,5-triphosphate receptor type 1 gene to enhance its expression. J Biol Chem. 2005 Oct 14; 280(41):35062-8.
    View in: PubMed
  16. Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, Cingolani G. Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha. J Biol Chem. 2005 Mar 18; 280(11):10599-606.
    View in: PubMed
  17. Temple V, Mapira P, Adeniyi K, Sims P. Iodine deficiency in Papua New Guinea (sub-clinical iodine deficiency and salt iodization in the highlands of Papua New Guinea). J Public Health (Oxf). 2005 Mar; 27(1):45-8.
    View in: PubMed
  18. Giddings KS, Zhao J, Sims PJ, Tweten RK. Human CD59 is a receptor for the cholesterol-dependent cytolysin intermedilysin. Nat Struct Mol Biol. 2004 Dec; 11(12):1173-8.
    View in: PubMed
  19. Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH. Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep; 78(17):8983-93.
    View in: PubMed
  20. Wiedmer T, Zhao J, Li L, Zhou Q, Hevener A, Olefsky JM, Curtiss LK, Sims PJ. Adiposity, dyslipidemia, and insulin resistance in mice with targeted deletion of phospholipid scramblase 3 (PLSCR3). Proc Natl Acad Sci U S A. 2004 Sep 7; 101(36):13296-301.
    View in: PubMed
  21. Zhao KW, Li X, Zhao Q, Huang Y, Li D, Peng ZG, Shen WZ, Zhao J, Zhou Q, Chen Z, Sims PJ, Wiedmer T, Chen GQ. Protein kinase Cdelta mediates retinoic acid and phorbol myristate acetate-induced phospholipid scramblase 1 gene expression: its role in leukemic cell differentiation. Blood. 2004 Dec 1; 104(12):3731-8.
    View in: PubMed
  22. Ben-Efraim I, Zhou Q, Wiedmer T, Gerace L, Sims PJ. Phospholipid scramblase 1 is imported into the nucleus by a receptor-mediated pathway and interacts with DNA. Biochemistry. 2004 Mar 30; 43(12):3518-26.
    View in: PubMed
  23. Nanjundan M, Sun J, Zhao J, Zhou Q, Sims PJ, Wiedmer T. Plasma membrane phospholipid scramblase 1 promotes EGF-dependent activation of c-Src through the epidermal growth factor receptor. J Biol Chem. 2003 Sep 26; 278(39):37413-8.
    View in: PubMed
  24. Rami A, Sims J, Botez G, Winckler J. Spatial resolution of phospholipid scramblase 1 (PLSCR1), caspase-3 activation and DNA-fragmentation in the human hippocampus after cerebral ischemia. Neurochem Int. 2003 Jul; 43(1):79-87.
    View in: PubMed
  25. Kametaka S, Shibata M, Moroe K, Kanamori S, Ohsawa Y, Waguri S, Sims PJ, Emoto K, Umeda M, Uchiyama Y. Identification of phospholipid scramblase 1 as a novel interacting molecule with beta -secretase (beta -site amyloid precursor protein (APP) cleaving enzyme (BACE)). J Biol Chem. 2003 Apr 25; 278(17):15239-45.
    View in: PubMed
  26. Wiedmer T, Zhao J, Nanjundan M, Sims PJ. Palmitoylation of phospholipid scramblase 1 controls its distribution between nucleus and plasma membrane. Biochemistry. 2003 Feb 11; 42(5):1227-33.
    View in: PubMed
  27. de Vries KJ, Wiedmer T, Sims PJ, Gadella BM. Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells. Biol Reprod. 2003 Jun; 68(6):2122-34.
    View in: PubMed
  28. Zhou Q, Zhao J, Wiedmer T, Sims PJ. Normal hemostasis but defective hematopoietic response to growth factors in mice deficient in phospholipid scramblase 1. Blood. 2002 Jun 1; 99(11):4030-8.
    View in: PubMed
  29. Sun J, Nanjundan M, Pike LJ, Wiedmer T, Sims PJ. Plasma membrane phospholipid scramblase 1 is enriched in lipid rafts and interacts with the epidermal growth factor receptor. Biochemistry. 2002 May 21; 41(20):6338-45.
    View in: PubMed
  30. Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ. Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15; 62(2):397-402.
    View in: PubMed
  31. Sims PJ, Wiedmer T. Unraveling the mysteries of phospholipid scrambling. Thromb Haemost. 2001 Jul; 86(1):266-75.
    View in: PubMed
  32. Sun J, Zhao J, Schwartz MA, Wang JY, Wiedmer T, Sims PJ. c-Abl tyrosine kinase binds and phosphorylates phospholipid scramblase 1. J Biol Chem. 2001 Aug 3; 276(31):28984-90.
    View in: PubMed
  33. Presta L, Sims P, Meng YG, Moran P, Bullens S, Bunting S, Schoenfeld J, Lowe D, Lai J, Rancatore P, Iverson M, Lim A, Chisholm V, Kelley RF, Riederer M, Kirchhofer D. Generation of a humanized, high affinity anti-tissue factor antibody for use as a novel antithrombotic therapeutic. Thromb Haemost. 2001 Mar; 85(3):379-89.
    View in: PubMed
  34. Wiedmer T, Zhou Q, Kwoh DY, Sims PJ. Identification of three new members of the phospholipid scramblase gene family. Biochim Biophys Acta. 2000 Jul 31; 1467(1):244-53.
    View in: PubMed
  35. Tepper AD, Ruurs P, Wiedmer T, Sims PJ, Borst J, van Blitterswijk WJ. Sphingomyelin hydrolysis to ceramide during the execution phase of apoptosis results from phospholipid scrambling and alters cell-surface morphology. J Cell Biol. 2000 Jul 10; 150(1):155-64.
    View in: PubMed
  36. Zhou Q, Zhao J, Al-Zoghaibi F, Zhou A, Wiedmer T, Silverman RH, Sims PJ. Transcriptional control of the human plasma membrane phospholipid scramblase 1 gene is mediated by interferon-alpha. Blood. 2000 Apr 15; 95(8):2593-9.
    View in: PubMed
  37. Fadeel B, Gleiss B, Högstrand K, Chandra J, Wiedmer T, Sims PJ, Henter JI, Orrenius S, Samali A. Phosphatidylserine exposure during apoptosis is a cell-type-specific event and does not correlate with plasma membrane phospholipid scramblase expression. Biochem Biophys Res Commun. 1999 Dec 20; 266(2):504-11.
    View in: PubMed
  38. Wilson HA, Waldrip JB, Nielson KH, Judd AM, Han SK, Cho W, Sims PJ, Bell JD. Mechanisms by which elevated intracellular calcium induces S49 cell membranes to become susceptible to the action of secretory phospholipase A2. J Biol Chem. 1999 Apr 23; 274(17):11494-504.
    View in: PubMed
  39. Stout JG, Zhou Q, Wiedmer T, Sims PJ. Change in conformation of plasma membrane phospholipid scramblase induced by occupancy of its Ca2+ binding site. Biochemistry. 1998 Oct 20; 37(42):14860-6.
    View in: PubMed
  40. Zhou Q, Sims PJ, Wiedmer T. Expression of proteins controlling transbilayer movement of plasma membrane phospholipids in the B lymphocytes from a patient with Scott syndrome. Blood. 1998 Sep 1; 92(5):1707-12.
    View in: PubMed
  41. Zhao J, Zhou Q, Wiedmer T, Sims PJ. Palmitoylation of phospholipid scramblase is required for normal function in promoting Ca2+-activated transbilayer movement of membrane phospholipids. Biochemistry. 1998 May 5; 37(18):6361-6.
    View in: PubMed
  42. Zhao XJ, Zhao J, Zhou Q, Sims PJ. Identity of the residues responsible for the species-restricted complement inhibitory function of human CD59. J Biol Chem. 1998 Apr 24; 273(17):10665-71.
    View in: PubMed
  43. Zhao J, Zhou Q, Wiedmer T, Sims PJ. Level of expression of phospholipid scramblase regulates induced movement of phosphatidylserine to the cell surface. J Biol Chem. 1998 Mar 20; 273(12):6603-6.
    View in: PubMed
  44. Zhou Q, Sims PJ, Wiedmer T. Identity of a conserved motif in phospholipid scramblase that is required for Ca2+-accelerated transbilayer movement of membrane phospholipids. Biochemistry. 1998 Feb 24; 37(8):2356-60.
    View in: PubMed
  45. Zhou Q, Zhao J, Stout JG, Luhm RA, Wiedmer T, Sims PJ. Molecular cloning of human plasma membrane phospholipid scramblase. A protein mediating transbilayer movement of plasma membrane phospholipids. J Biol Chem. 1997 Jul 18; 272(29):18240-4.
    View in: PubMed
  46. Zhao J, Sims PJ, Wiedmer T. Production and characterization of a mutant cell line defective in aminophospholipid translocase. Biochim Biophys Acta. 1997 Jun 5; 1357(1):57-64.
    View in: PubMed
  47. Stout JG, Bassé F, Luhm RA, Weiss HJ, Wiedmer T, Sims PJ. Scott syndrome erythrocytes contain a membrane protein capable of mediating Ca2+-dependent transbilayer migration of membrane phospholipids. J Clin Invest. 1997 May 1; 99(9):2232-8.
    View in: PubMed
  48. Christiansen VJ, Sims PJ, Hamilton KK. Complement C5b-9 increases plasminogen binding and activation on human endothelial cells. Arterioscler Thromb Vasc Biol. 1997 Jan; 17(1):164-71.
    View in: PubMed
  49. Zhou Q, Zhao J, Hüsler T, Sims PJ. Expression of recombinant CD59 with an N-terminal peptide epitope facilitates analysis of residues contributing to its complement-inhibitory function. Mol Immunol. 1996 Oct; 33(14):1127-34.
    View in: PubMed
  50. Rother RP, Zhao J, Zhou Q, Sims PJ. Elimination of potential sites of glycosylation fails to abrogate complement regulatory function of cell surface CD59. J Biol Chem. 1996 Sep 27; 271(39):23842-5.
    View in: PubMed
  51. Bassé F, Stout JG, Sims PJ, Wiedmer T. Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid. J Biol Chem. 1996 Jul 19; 271(29):17205-10.
    View in: PubMed
  52. Husler T, Lockert DH, Sims PJ. Role of a disulfide-bonded peptide loop within human complement C9 in the species-selectivity of complement inhibitor CD59. Biochemistry. 1996 Mar 12; 35(10):3263-9.
    View in: PubMed
  53. Petranka J, Zhao J, Norris J, Tweedy NB, Ware RE, Sims PJ, Rosse WF. Structure-function relationships of the complement regulatory protein, CD59. Blood Cells Mol Dis. 1996; 22(3):281-96.
    View in: PubMed
  54. Sims PJ, Wiedmer T. Induction of cellular procoagulant activity by the membrane attack complex of complement. Semin Cell Biol. 1995 Oct; 6(5):275-82.
    View in: PubMed
  55. Bevers EM, Wiedmer T, Comfurius P, Zhao J, Smeets EF, Schlegel RA, Schroit AJ, Weiss HJ, Williamson P, Zwaal RF, Sims PJ. The complex of phosphatidylinositol 4,5-bisphosphate and calcium ions is not responsible for Ca2+-induced loss of phospholipid asymmetry in the human erythrocyte: a study in Scott syndrome, a disorder of calcium-induced phospholipid scrambling. Blood. 1995 Sep 1; 86(5):1983-91.
    View in: PubMed
  56. Lockert DH, Kaufman KM, Chang CP, Hüsler T, Sodetz JM, Sims PJ. Identity of the segment of human complement C8 recognized by complement regulatory protein CD59. J Biol Chem. 1995 Aug 25; 270(34):19723-8.
    View in: PubMed
  57. Hüsler T, Lockert DH, Kaufman KM, Sodetz JM, Sims PJ. Chimeras of human complement C9 reveal the site recognized by complement regulatory protein CD59. J Biol Chem. 1995 Feb 24; 270(8):3483-6.
    View in: PubMed
  58. Kojima H, Newton-Nash D, Weiss HJ, Zhao J, Sims PJ, Wiedmer T. Production and characterization of transformed B-lymphocytes expressing the membrane defect of Scott syndrome. J Clin Invest. 1994 Dec; 94(6):2237-44.
    View in: PubMed
  59. Chang CP, Hüsler T, Zhao J, Wiedmer T, Sims PJ. Identity of a peptide domain of human C9 that is bound by the cell-surface complement inhibitor, CD59. J Biol Chem. 1994 Oct 21; 269(42):26424-30.
    View in: PubMed
  60. Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE. CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15; 153(6):2444-56.
    View in: PubMed
  61. Kennedy SP, Rollins SA, Burton WV, Sims PJ, Bothwell AL, Squinto SP, Zavoico GB. Protection of porcine aortic endothelial cells from complement-mediated cell lysis and activation by recombinant human CD59. Transplantation. 1994 May 27; 57(10):1494-501.
    View in: PubMed
  62. Wiedmer T, Hall SE, Ortel TL, Kane WH, Rosse WF, Sims PJ. Complement-induced vesiculation and exposure of membrane prothrombinase sites in platelets of paroxysmal nocturnal hemoglobinuria. Blood. 1993 Aug 15; 82(4):1192-6.
    View in: PubMed
  63. Chang CP, Zhao J, Wiedmer T, Sims PJ. Contribution of platelet microparticle formation and granule secretion to the transmembrane migration of phosphatidylserine. J Biol Chem. 1993 Apr 5; 268(10):7171-8.
    View in: PubMed
  64. Hamilton KK, Zhao J, Sims PJ. Interaction between apolipoproteins A-I and A-II and the membrane attack complex of complement. Affinity of the apoproteins for polymeric C9. J Biol Chem. 1993 Feb 15; 268(5):3632-8.
    View in: PubMed
  65. Dahlbäck B, Wiedmer T, Sims PJ. Binding of anticoagulant vitamin K-dependent protein S to platelet-derived microparticles. Biochemistry. 1992 Dec 29; 31(51):12769-77.
    View in: PubMed
  66. Shattil SJ, Cunningham M, Wiedmer T, Zhao J, Sims PJ, Brass LF. Regulation of glycoprotein IIb-IIIa receptor function studied with platelets permeabilized by the pore-forming complement proteins C5b-9. J Biol Chem. 1992 Sep 15; 267(26):18424-31.
    View in: PubMed
  67. Braga LL, Ninomiya H, McCoy JJ, Eacker S, Wiedmer T, Pham C, Wood S, Sims PJ, Petri WA. Inhibition of the complement membrane attack complex by the galactose-specific adhesion of Entamoeba histolytica. J Clin Invest. 1992 Sep; 90(3):1131-7.
    View in: PubMed
  68. Ninomiya H, Sims PJ. The human complement regulatory protein CD59 binds to the alpha-chain of C8 and to the "b"domain of C9. J Biol Chem. 1992 Jul 5; 267(19):13675-80.
    View in: PubMed
  69. Hahn WC, Menu E, Bothwell AL, Sims PJ, Bierer BE. Overlapping but nonidentical binding sites on CD2 for CD58 and a second ligand CD59. Science. 1992 Jun 26; 256(5065):1805-7.
    View in: PubMed
  70. Ninomiya H, Stewart BH, Rollins SA, Zhao J, Bothwell AL, Sims PJ. Contribution of the N-linked carbohydrate of erythrocyte antigen CD59 to its complement-inhibitory activity. J Biol Chem. 1992 Apr 25; 267(12):8404-10.
    View in: PubMed
  71. Bevers EM, Wiedmer T, Comfurius P, Shattil SJ, Weiss HJ, Zwaal RF, Sims PJ. Defective Ca(2+)-induced microvesiculation and deficient expression of procoagulant activity in erythrocytes from a patient with a bleeding disorder: a study of the red blood cells of Scott syndrome. Blood. 1992 Jan 15; 79(2):380-8.
    View in: PubMed
  72. Braga LL, Ninomiya H, McCoy JJ, Adal K, Wiedmer T, Pham C, Sims PJ, Petri WA. Inhibition of the complement membrane attack complex by the galactose-specific adhesin of Entamoeba histolytica. Arch Med Res. 1992; 23(2):133.
    View in: PubMed
  73. Hamilton KK, Sims PJ. The terminal complement proteins C5b-9 augment binding of high density lipoprotein and its apolipoproteins A-I and A-II to human endothelial cells. J Clin Invest. 1991 Dec; 88(6):1833-40.
    View in: PubMed
  74. Wiedmer T, Sims PJ. Participation of protein kinases in complement C5b-9-induced shedding of platelet plasma membrane vesicles. Blood. 1991 Dec 1; 78(11):2880-6.
    View in: PubMed
  75. Gilbert GE, Sims PJ, Wiedmer T, Furie B, Furie BC, Shattil SJ. Platelet-derived microparticles express high affinity receptors for factor VIII. J Biol Chem. 1991 Sep 15; 266(26):17261-8.
    View in: PubMed
  76. Sims PJ, Wiedmer T. The response of human platelets to activated components of the complement system. Immunol Today. 1991 Sep; 12(9):338-42.
    View in: PubMed
  77. Zhao J, Rollins SA, Maher SE, Bothwell AL, Sims PJ. Amplified gene expression in CD59-transfected Chinese hamster ovary cells confers protection against the membrane attack complex of human complement. J Biol Chem. 1991 Jul 15; 266(20):13418-22.
    View in: PubMed
  78. Tweten RK, Harris RW, Sims PJ. Isolation of a tryptic fragment from Clostridium perfringens theta-toxin that contains sites for membrane binding and self-aggregation. J Biol Chem. 1991 Jul 5; 266(19):12449-54.
    View in: PubMed
  79. Harris RW, Sims PJ, Tweten RK. Evidence that Clostridium perfringens theta-toxin induces colloid-osmotic lysis of erythrocytes. Infect Immun. 1991 Jul; 59(7):2499-501.
    View in: PubMed
  80. Sims PJ, Ginsberg MH, Plow EF, Shattil SJ. Effect of platelet activation on the conformation of the plasma membrane glycoprotein IIb-IIIa complex. J Biol Chem. 1991 Apr 25; 266(12):7345-52.
    View in: PubMed
  81. Harris RW, Sims PJ, Tweten RK. Kinetic aspects of the aggregation of Clostridium perfringens theta-toxin on erythrocyte membranes. A fluorescence energy transfer study. J Biol Chem. 1991 Apr 15; 266(11):6936-41.
    View in: PubMed
  82. Rollins SA, Zhao J, Ninomiya H, Sims PJ. Inhibition of homologous complement by CD59 is mediated by a species-selective recognition conferred through binding to C8 within C5b-8 or C9 within C5b-9. J Immunol. 1991 Apr 1; 146(7):2345-51.
    View in: PubMed
  83. Gerrard JM, Lint D, Sims PJ, Wiedmer T, Fugate RD, McMillan E, Robertson C, Israels SJ. Identification of a platelet dense granule membrane protein that is deficient in a patient with the Hermansky-Pudlak syndrome. Blood. 1991 Jan 1; 77(1):101-12.
    View in: PubMed
  84. Hamilton KK, Ji Z, Rollins S, Stewart BH, Sims PJ. Regulatory control of the terminal complement proteins at the surface of human endothelial cells: neutralization of a C5b-9 inhibitor by antibody to CD59. Blood. 1990 Dec 15; 76(12):2572-7.
    View in: PubMed
  85. Rollins SA, Sims PJ. The complement-inhibitory activity of CD59 resides in its capacity to block incorporation of C9 into membrane C5b-9. J Immunol. 1990 May 1; 144(9):3478-83.
    View in: PubMed
  86. Hamilton KK, Hattori R, Esmon CT, Sims PJ. Complement proteins C5b-9 induce vesiculation of the endothelial plasma membrane and expose catalytic surface for assembly of the prothrombinase enzyme complex. J Biol Chem. 1990 Mar 5; 265(7):3809-14.
    View in: PubMed
  87. Wiedmer T, Shattil SJ, Cunningham M, Sims PJ. Role of calcium and calpain in complement-induced vesiculation of the platelet plasma membrane and in the exposure of the platelet factor Va receptor. Biochemistry. 1990 Jan 23; 29(3):623-32.
    View in: PubMed
  88. Sims PJ, Rollins SA, Wiedmer T. Regulatory control of complement on blood platelets. Modulation of platelet procoagulant responses by a membrane inhibitor of the C5b-9 complex. J Biol Chem. 1989 Nov 15; 264(32):19228-35.
    View in: PubMed
  89. Van der Meer BW, Fugate RD, Sims PJ. Complement proteins C5b-9 induce transbilayer migration of membrane phospholipids. Biophys J. 1989 Nov; 56(5):935-46.
    View in: PubMed
  90. Sims PJ, Wiedmer T, Esmon CT, Weiss HJ, Shattil SJ. Assembly of the platelet prothrombinase complex is linked to vesiculation of the platelet plasma membrane. Studies in Scott syndrome: an isolated defect in platelet procoagulant activity. J Biol Chem. 1989 Oct 15; 264(29):17049-57.
    View in: PubMed
  91. Hattori R, Hamilton KK, McEver RP, Sims PJ. Complement proteins C5b-9 induce secretion of high molecular weight multimers of endothelial von Willebrand factor and translocation of granule membrane protein GMP-140 to the cell surface. J Biol Chem. 1989 May 25; 264(15):9053-60.
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  92. Hattori R, Hamilton KK, Fugate RD, McEver RP, Sims PJ. Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140. J Biol Chem. 1989 May 15; 264(14):7768-71.
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  93. Ando B, Wiedmer T, Sims PJ. The secretory release reaction initiated by complement proteins C5b-9 occurs without platelet aggregation through glycoprotein IIb-IIIa. Blood. 1989 Feb; 73(2):462-7.
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  94. Sims P, James C, Broda P. The identification, molecular cloning and characterisation of a gene from Phanerochaete chrysosporium that shows strong homology to the exo-cellobiohydrolase I gene from Trichoderma reesei. Gene. 1988 Dec 30; 74(2):411-22.
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  95. Sims PJ, Faioni EM, Wiedmer T, Shattil SJ. Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity. J Biol Chem. 1988 Dec 5; 263(34):18205-12.
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  96. Ando B, Wiedmer T, Hamilton KK, Sims PJ. Complement proteins C5b-9 initiate secretion of platelet storage granules without increased binding of fibrinogen or von Willebrand factor to newly expressed cell surface GPIIb-IIIa. J Biol Chem. 1988 Aug 25; 263(24):11907-14.
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  97. Stearns DJ, Kurosawa S, Sims PJ, Esmon NL, Esmon CT. The interaction of a Ca2+-dependent monoclonal antibody with the protein C activation peptide region. Evidence for obligatory Ca2+ binding to both antigen and antibody. J Biol Chem. 1988 Jan 15; 263(2):826-32.
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  98. Wiedmer T, Ando B, Sims PJ. Complement C5b-9-stimulated platelet secretion is associated with a Ca2+-initiated activation of cellular protein kinases. J Biol Chem. 1987 Oct 5; 262(28):13674-81.
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  99. Hamilton KK, Sims PJ. Changes in cytosolic Ca2+ associated with von Willebrand factor release in human endothelial cells exposed to histamine. Study of microcarrier cell monolayers using the fluorescent probe indo-1. J Clin Invest. 1987 Feb; 79(2):600-8.
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  100. Wiedmer T, Esmon CT, Sims PJ. On the mechanism by which complement proteins C5b-9 increase platelet prothrombinase activity. J Biol Chem. 1986 Nov 5; 261(31):14587-92.
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  101. Wiedmer T, Esmon CT, Sims PJ. Complement proteins C5b-9 stimulate procoagulant activity through platelet prothrombinase. Blood. 1986 Oct; 68(4):875-80.
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  102. Sims PJ, Wiedmer T. Repolarization of the membrane potential of blood platelets after complement damage: evidence for a Ca++ -dependent exocytotic elimination of C5b-9 pores. Blood. 1986 Aug; 68(2):556-61.
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  103. Benz R, Schmid A, Wiedmer T, Sims PJ. Single-channel analysis of the conductance fluctuations induced in lipid bilayer membranes by complement proteins C5b-9. J Membr Biol. 1986; 94(1):37-45.
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  104. Wiedmer T, Sims PJ. Effect of complement proteins C5b-9 on blood platelets. Evidence for reversible depolarization of membrane potential. J Biol Chem. 1985 Jul 5; 260(13):8014-9.
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  105. Cheng KH, Wiedmer T, Sims PJ. Fluorescence resonance energy transfer study of the associative state of membrane-bound complexes of complement proteins C5b-8. J Immunol. 1985 Jul; 135(1):459-64.
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  106. Parker CJ, Wiedmer T, Sims PJ, Rosse WF. Characterization of the complement sensitivity of paroxysmal nocturnal hemoglobinuria erythrocytes. J Clin Invest. 1985 Jun; 75(6):2074-84.
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  107. Wiedmer T, Sims PJ. Cyanine dye fluorescence used to measure membrane potential changes due to the assembly of complement proteins C5b-9. J Membr Biol. 1985; 84(3):249-58.
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  108. Sims PJ. Complement protein C9 labeled with fluorescein isothiocyanate can be used to monitor C9 polymerization and formation of the cytolytic membrane lesion. Biochemistry. 1984 Jul 3; 23(14):3248-60.
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  109. Sims PJ, Wiedmer T. Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8. Biochemistry. 1984 Jul 3; 23(14):3260-7.
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  110. Sims PJ, Wiedmer T. The influence of electrochemical gradients of Na+ and K+ upon the membrane binding and pore forming activity of the terminal complement proteins. J Membr Biol. 1984; 78(2):169-76.
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  111. Glatt H, Friedberg T, Grover PL, Sims P, Oesch F. Inactivation of a diol-epoxide and a K-region epoxide with high efficiency by glutathione transferase X. Cancer Res. 1983 Dec; 43(12 Pt 1):5713-7.
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  112. Hodgson RM, Cary PD, Grover PL, Sims P. Metabolic activation of chrysene by hamster embryo cells: evidence for the formation of a 'bay-region' diol-epoxide-N2-guanine adduct in RNA. Carcinogenesis. 1983 Sep; 4(9):1153-8.
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  113. Sims PJ, Boswell EB. The measurement of platelet-associated IgG by a microplate quantitative antiglobulin consumption assay using automated through-the-well spectrophotometry. J Lab Clin Med. 1983 Sep; 102(3):352-60.
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  114. Sims PJ. Complement pores in erythrocyte membranes. Analysis of C8/C9 binding required for functional membrane damage. Biochim Biophys Acta. 1983 Aug 10; 732(3):541-52.
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  115. Weston A, Grover PL, Sims P. Formation of the 11,12-diol as a metabolite of benzo(a)pyrene by rat skin in vivo. Biochem Biophys Res Commun. 1982 Apr 14; 105(3):935-41.
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  116. Sims PJ, Boswell EB. Elevated platelet-bound IgG associated with an episode of thrombotic thrombocytopenic purpura. Blood. 1981 Oct; 58(4):682-4.
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  117. Sims PJ. Permeability characteristics of complement-damaged membranes: evaluation of the membrane leak generated by the complement proteins C5b-9. Proc Natl Acad Sci U S A. 1981 Mar; 78(3):1838-42.
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  118. Sims PJ, Lauf PK. Analysis of solute diffusion across the C5b-9 membrane lesion of complement: evidence that individual C5b-9 complexes do not function as discrete, uniform pores. J Immunol. 1980 Dec; 125(6):2617-25.
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  119. MacNicoll AD, Easty GC, Neville AM, Grover PL, Sims P. Metabolism and activation of carcinogenic polycyclic hydrocarbons by human mammary cells. Biochem Biophys Res Commun. 1980 Aug 29; 95(4):1599-606.
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  120. Pal K, Grover PL, Sims P. The induction of sister-chromatid exchanges in Chinese hamster ovary cells by some epoxides and phenolic derivatives of benzo[a]pyrene. Mutat Res. 1980 Jun; 78(2):193-9.
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  121. Pal K, Grover PL, Sims P. The metabolism of benzo(a)pyrene by Chinese hamster ovary cells in culture. Xenobiotica. 1980 Jan; 10(1):25-31.
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  122. Vigny P, Kindts M, Duquesne M, Cooper CS, Grover PL, Sims P. Metabolic activation of benz(a)anthracene: fluorescence spectral evidence indicates the involvement of a non-'bay-region' diol-epoxide. Carcinogenesis. 1980 Jan; 1(1):33-6.
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  123. MacNicoll AD, Cooper CS, Ribeiro O, Gervasi PG, Hewer A, Walsh C, Grover PL, Sims P. The involvement of a non-'bay-region' diol-epoxide in the formation of benz(a)anthracene-DNA adducts in a rat-liver microsomal system. Biochem Biophys Res Commun. 1979 Nov 28; 91(2):490-7.
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  124. Malaveille C, Bartsch H, Marquardt H, Baker S, Tierney B, Hewer A, Grover PL, Sims P. Metabolic activation of 3-methylcholanthrene: mutagenic and transforming activities of the 9,10-dihydrodiol. Biochem Biophys Res Commun. 1978 Dec 29; 85(4):1568-74.
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  125. Marquardt H, Baker S, Tierney B, Grover PL, Sims P. Induction of malignant transformation and mutagenesis by dihydrodiols derived from 7,12-dimethylbenz[a]anthracene. Biochem Biophys Res Commun. 1978 Nov 14; 85(1):357-62.
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  126. Sims PJ, Lauf PK. Steady-state analysis of tracer exchange across the C5b-9 complement lesion in a biological membrane. Proc Natl Acad Sci U S A. 1978 Nov; 75(11):5669-73.
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  127. Malaveille C, Bartsch H, Tierney B, Grover PL, Sims P. Microsome-mediated mutagenicities of the dihydrodiols of 7,12-dimethylbenz[a]anthracene: high mutagenic activity of the 3,4-dihydrodiol. Biochem Biophys Res Commun. 1978 Aug 29; 83(4):1468-73.
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  128. Pal K, Tierney B, Grover PL, Sims P. Induction of sister-chromatid exchanges in Chinese hamster ovary cells treated in vitro with non-K-region dihydrodiols of 7-methylbenz[a]anthracene and benzo[a]pyrene. Mutat Res. 1978 Jun; 50(3):367-75.
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  129. Flaks A, Sims P. The activity of 7-methylbenz(a)anthracene metabolites in an in vitro-in vivo carcinogenicity test using mouse lung tissue. Cancer Lett. 1977 Sep; 3(3-4):163-7.
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  130. Malaveille C, Tierney B, Grover PL, Sims P, Bartsch H. High microsome-mediated mutagenicity of the 3,4-dihydrodiol of 7-methylbenz[a]anthracene in S. typhimurium TA 98. Biochem Biophys Res Commun. 1977 Mar 21; 75(2):427-33.
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  131. Baird WM, Grover PL, Sims P, Brookes P. Comparison of the products of the reaction of 7-methylbenz(a)anthracene 5,6-oxide and RNA, with those formed in 7-methylbenz(a)anthracene-treated cells. Cancer Res. 1976 Jul; 36(7 PT 1):2306-11.
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  132. Marquardt H, Grover PL, Sims P. In vitro malignant transformation of mouse fibroblasts by non-K-region dihydrodiols derived from 7-methylbenz(a)anthracene, 7,12-dimethylbenz(a)anthracene, and benzo(a)pyrene. Cancer Res. 1976 Jun; 36(6):2059-64.
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  133. Malaveille C, Bartsch H, Grover PL, Sims P. Mutagenicity of non-K-region diols and diol-epoxides of benz(a)anthracene and benzo(a)pyrene in S. typhimurium TA 100. Biochem Biophys Res Commun. 1975 Sep 16; 66(2):693-700.
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  134. Sims PJ, Waggoner AS, Wang CH, Hoffman JF. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry. 1974 Jul 30; 13(16):3315-30.
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  135. Marquardt H, Sodergren JE, Sims P, Grover PL. Malignant transformation in vitro of mouse fibroblasts by 7,12-dimethylbenz(A)anthracene and 7-hydroxymethylbenz(A)anthracene and by their K-region derivatives. Int J Cancer. 1974 Mar 15; 13(3):304-10.
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  136. Baird WM, Dipple A, Grover PL, Sims P, Brookes P. Studies on the formation of hydrocarbon-deoxyribonucleoside products by the binding of derivatives of 7-methylbenz(a)anthracene to DNA in aqueous solution and in mouse embryo cells in culture. Cancer Res. 1973 Oct; 33(10):2386-92.
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  137. Flaks A, Hamilton JM, Clayson DB, Sims P. Evaluation of the carcinogenic activity of the hydroxymethyl derivatives of 7,12-dimethylbenz(a)anthracene by an in vitro-in vivo technique. Int J Cancer. 1972 Nov; 10(3):548-51.
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  138. Huberman E, Kuroki T, Marquardt H, Selkirk JK, Heidelberger C, Grover PL, Sims P. Transformation of hamster embryo cells by epoxides and other derivatives of polycyclic hydrocarbons. Cancer Res. 1972 Jul; 32(7):1391-6.
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  139. Kuroki T, Huberman E, Marquardt H, Selkirk JK, Heidelberger C, Grover PL, Sims P. Binding of K-region epoxides and other derivatives of benz(a)anthracene and dibenz(a,h)anthracene to DNA, RNA, and proteins of transformable cells. Chem Biol Interact. 1972 May; 4(6):389-97.
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  140. Marquardt H, Kuroki T, Huberman E, Selkirk JK, Heidelberger C, Grover PL, Sims P. Malignant transformation of cells derived from mouse prostate by epoxides and other derivatives of polycyclic hydrocarbons. Cancer Res. 1972 Apr; 32(4):716-20.
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