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Mark Platt

TitleAssistant Professor
InstitutionSchool of Medicine and Dentistry
DepartmentMicrobiology and Immunology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 611
Rochester NY 14642
 
 Overview
"Elucidating protein structure and function is an important first step towards understanding every cellular process as well as the molecular basis of many diseases. My research into the field of proteomics, which is defined as the "attempt to identify all of the proteins expressed by a given genome," seeks to not only identify proteins through determination of their primary amino acid sequence, but to also characterize protein post-translational modifications (PTMs) and to quantitate their relative expression levels between different cell types and/or growth states. To this end, our laboratory has demonstrated expertise in the enrichment and preparation of samples from bacteria, plants, and mammalian systems (including human tissues and biological fluids) and we have developed several novel proteomic approaches for biomarker discovery and detection of potential drug targets. The instrumentation installed, maintained and operated within the laboratory includes a number of high-end mass spectrometers (Thermo LTQ, Thermo TSQ, Bruker micrOTOF and Bruker 9.4T Apex FTMS) and associated data analysis software (SEQUEST, Mascot, Proteome Discoverer). This equipment and expertise has permitted us to successfully establish joint research collaborations and integrate proteomic workflows into wider research efforts at the URMC."

 
 Selected Publications
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  1. Tang X, Platt MD, Lagnese CM, Leslie JR, Hamada FN. Temperature integration at the AC thermosensory neurons in Drosophila. J Neurosci. 2013 Jan 16; 33(3):894-901.
    View in: PubMed
  2. Porambo JR, Salicioni AM, Visconti PE, Platt MD. Sperm phosphoproteomics: historical perspectives and current methodologies. Expert Rev Proteomics. 2012 Oct; 9(5):533-48.
    View in: PubMed
  3. Platt MD, Salicioni AM, Hunt DF, Visconti PE. Use of differential isotopic labeling and mass spectrometry to analyze capacitation-associated changes in the phosphorylation status of mouse sperm proteins. J Proteome Res. 2009 Mar; 8(3):1431-40.
    View in: PubMed
  4. Platt MD, Schurr MJ, Sauer K, Vazquez G, Kukavica-Ibrulj I, Potvin E, Levesque RC, Fedynak A, Brinkman FS, Schurr J, Hwang SH, Lau GW, Limbach PA, Rowe JJ, Lieberman MA, Barraud N, Webb J, Kjelleberg S, Hunt DF, Hassett DJ. Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. J Bacteriol. 2008 Apr; 190(8):2739-58.
    View in: PubMed
  5. Salicioni AM, Platt MD, Wertheimer EV, Arcelay E, Allaire A, Sosnik J, Visconti PE. Signalling pathways involved in sperm capacitation. Soc Reprod Fertil Suppl. 2007; 65:245-59.
    View in: PubMed
  6. Lin CH, Platt MD, Ficarro SB, Hoofnagle MH, Shabanowitz J, Comai L, Hunt DF, Owens GK. Mass spectrometric identification of phosphorylation sites of rRNA transcription factor upstream binding factor. Am J Physiol Cell Physiol. 2007 May; 292(5):C1617-24.
    View in: PubMed
  7. Garcia BA, Platt MD, Born TL, Shabanowitz J, Marcus NA, Hunt DF. Protein profile of osteoarthritic human articular cartilage using tandem mass spectrometry. Rapid Commun Mass Spectrom. 2006; 20(20):2999-3006.
    View in: PubMed
  8. Hassett DJ, Limbach PA, Hennigan RF, Klose KE, Hancock RE, Platt MD, Hunt DF. Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targets. Expert Opin Biol Ther. 2003 Dec; 3(8):1201-7.
    View in: PubMed
  9. Jeffrey L, Platt MD. [Recent advances in xenotransplantation]. Presse Med. 1992 Dec 2; 21(41):1932-8.
    View in: PubMed

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