Field Test of MALDI
MALDI Field Test Results
- A dilution series of seven proteins were analyzed
- 1000 to 1 ng of protein were loaded in half-log dilution
| Protein | Mass (kDa) | pmoles of 1ug band | pmoles of 0.3 ug bands |  |
|---|---|---|---|---|
| ß-gal | 116 | 9 | 3 | |
| Albumin, serum | 66 | 15 | 5 | |
| Glut.Dehydrogenase | 61 | 16 | 5 | |
| peroxidase | 44 | 23 | 7 | |
| GPDH | 36 | 26 | 9 | |
| superoxide dismutase | 16 | 62 | 20 | |
| α-Lactalbumin | 14 | 71 | 22 |
- MALDI-TOF analysis of all protein bands ( > 312 ng ) produced excellent quality and publishable protein identifications.
- 312 ng is not our limit of detection; however, this is what we currently guarantee by MALDI.
Alternative Mass Spectrometry Methods:
For increased coverage and sensitivity, samples can be simultaneously applied to our:
- Nanospray LC-MS/MS instruments. (Method # 40, 50)
- Integrated HPLC, robot-spotter, high-throughput MALDI-TOF/TOF service (LC-MALDI, Method # 30)
