Pricing
| Experiment | Details | Set-Up Fee | Price per sample | Total Price for 1 sample | Total Price for X samples |
| Pre-Digestion Processing | |||||
| Low pH Elution | Elution of Proteins From Beads | $15 | $10 | $25 | $15 + $10X |
| Protein Quantitation | For Protein Quant of Cell Lysates | $40 | $10 | $50 | $40 + $10X |
| Silver Destain | Required for Gel Digest | $15 | $10 | $25 | $15 + $20X |
| Dialysis | Required for High Salt/High Volume | $40 | $20 | $60 | $40 + $20X |
| Digestion | |||||
| Liquid Digest | Trypsin Digest of In Solution Sample | $30 | $5 | $35 | $30 + $5X |
| On Bead Digest | Trypsin Digest of Bead Sample | $30 | $5 | $35 | $30+ $5X |
| Gel Digest | Trypsin Digest of Gel Isolated Band | $60 | $15 | $75 | $60 + $15X |
| Alternate Enzyme | Chymotrypsin, Glu-C, etc. | $25 | - | $25 | $25 + above |
| Post Digestion Processing | |||||
| Desalting | Required for MALDI | $25 | $10 | $35 | $25 + $10X |
| Strong Cation Exchange | Detergent Removal | $25 | $10 | $35 | $25 + $10X |
| Strong Cation Exchange | Gradient Elution Into 5 Fractions | $50 | $25 | $75 | $50 + $25X |
| Phospho Enrichment | Isolation of Phosphopeptides | $75 | $25 | $100 | $75 + $25X |
| Mass Spectrometry | |||||
| MALDI MS | MW Scan | $50 | $25 | $75 | $50 + $25X |
| MALDI MS/MS | ID 1-2 Pure Proteins | $50 | $50 | $100 | $50 + $50X |
| LCQ Run | 3-30 Proteins | - | $75 | $75 | $375 |
| LTQ 80 Minute Run | 3-150 Proteins | - | $100 | $100 | $500 |
| LTQ 120 Minute Run | >150 Proteins | - | $175 | $175 | $875 |
| LTQ 180 Minute Run | >200 Proteins | - | $250 | $250 | $1,250 |
| Analysis | |||||
| PTM Search | Phosphorylation, Acetylation, etc. | - | $30/each | $30 | $150 |
| Additional MASCOT Search | Different Species Search | - | $20/each | $20 | $100 |
| Custom Database Search | Custom Database Search | - | $20/each | $20 | $100 |
| Detailed Analysis | Additional Custom Reports | - | $30/hr | $30 | $30+ $30x |
| Custom Mass Spec Services | Please contact Fred Hagen for a consultation | inquire |
NOTE: Add 54% to fee if outside U of R and not paid with NIH grant
| Typical Services | MALDI is lowest cost MS service for many samples. LTQ analysis produces highest quality and coverage data. | |||||
| Goals of Service | # of samples | Subtotal for digest | Subtotal for processing | Subtotal for MS analysis | Total fee | |
| ID Pure Protein (>100ng) | MALDI-TOF/TOF of a gel slice (+ desalting) | 1 | $75 | $35 | $100 | $210 |
| ID Pure Protein (>100ng) | MALDI-TOF/TOF of a gel slice (+ desalting) | 5 | $120 | $85 | $300 | $505 |
| ID Protein (<100ng) | LC-MS/MS analysis of a gel slice on an LCQ | 1 | $75 | - | $75 | $150 |
| ID protein (>sensitivity) | LC-MS/MS analysis of a gel slice on an LTQ | 1 | $75 | - | $100 | $175 |
| ID proteins in a complex | LC-MS/MS analysis of a pull-down on an LTQ | 2 | $40 | - | $200 | $240 |
| Map PTM on a protein | LC-MS/MS analysis on an LTQ (with 2 proteases) | 2 | $65 | - | $260 | $325 |
This cost breakdown is very accurate.
Occasionally some additional services may need to be added to optimize your results – if changes need to be made after submitting your samples, then you will be contacted to authorization any additional charges.
For custom services, prices are project dependent – please see Fred Hagen
PLEASE NOTE :
These prices are for NIH grant holders and also for investigators with a University of Rochester grant account. If you pay with neither of these types of grants/accounts, then add 54% to fee.
