Techniques

Research papers listed below were published by University of Rochester researchers with the technical support of the Electron Microscope Research Core.

The following techniques are available for Electron Microscope processing and analysis:

Routine Processing For Electron Microscopy

1. Miano JM, Ramanan N, Georger MA, de Mesy Bentley KL, Emerson RL, Balza RO Jr, Xiao Q, Weiler H, Ginty DD, Misra RP.  Restricted inactivation of serum response factor to the cardiovascular system.  Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17132-7. Epub 2004 Nov 29.
2. Lantum HB, Baggs RB, Krenitsky DM, Anders MW.  Nephrotoxicity of chlorofluoroacetic acid in rats.  Toxicol Sci. 2002 Dec;70(2):261-8.
3. Mason DA, Fleming RJ, Goldfarb DS.  Drosophila melanogaster importin alpha1 and alpha3 can replace importin alpha2 during spermatogenesis but not oogenesis.  Genetics. 2002 May;161(1):157-70.
4. O'Reilly MA, Staversky RJ, Huyck HL, Watkins RH, LoMonaco MB, D'Angio CT, Baggs RB, Maniscalco WM, Pryhuber GS.  Bcl-2 family gene expression during severe hyperoxia induced lung injury.  Lab Invest. 2000 Dec;80(12):1845-54.
5. Roper JM, Staversky RJ, Finkelstein JN, Keng PC, O'Reilly MA.  Identification and isolation of mouse type II cells on the basis of intrinsic expression of enhanced green fluorescent protein.  Am J Physiol Lung Cell Mol Physiol. 2003 Sep;285(3):L691-700. Epub 2003 May 9.

Morphometric Processing and Image Analysis

1. Qin F, Rounds NK, Mao W, Kawai K, Liang CS.  Antioxidant vitamins prevent cardiomyocyte apoptosis produced by norepinephrine infusion in ferrets.  Cardiovasc Res. 2001 Sep;51(4):736-48.

Detection of Antibody/Antigen Reactions by Immunoelectron Microscopy Using Protein A Gold and gold tagged secondary antibodies, as well as Pre-embedding antibody/antigen labeling with the chromagen DAB which is silver enhanced.

1. Harvey DM, Calkins DJ.  Localization of kainate receptors to the presynaptic active zone of the rod photoreceptor in primate retina.  Vis Neurosci. 2002 Sep-Oct;19(5):681-92.
2. Yao PJ, Coleman PD, Calkins DJ.  High-resolution localization of clathrin assembly protein AP180 in the presynaptic terminals of mammalian neurons.  J Comp Neurol. 2002 May 27;447(2):152-62.
3. Sowden MP, Ballatori N, Jensen KL, Reed LH, Smith HC.  The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors.  J Cell Sci. 2002 Mar 1;115(Pt 5):1027-39.
4. Phung TL, Roncone A, Jensen KL, Sparks CE, Sparks JD.  Phosphoinositide 3-kinase activity is necessary for insulin-dependent inhibition of apolipoprotein B secretion by rat hepatocytes and localizes to the endoplasmic reticulum.  J Biol Chem. 1997 Dec 5;272(49):30693-702.

Visualization of Viral Particles by Negative Staining Electron Microscopy

1. Warzecha H, Mason HS, Lane C, Tryggvesson A, Rybicki E, Williamson AL, Clements JD, Rose RC.  Oral immunogenicity of human papillomavirus-like particles expressed in potato.  J Virol. 2003 Aug;77(16):8702-11.
2. Bal HP, Chroboczek J, Schoehn G, Ruigrok RW, Dewhurst S.  Adenovirus type 7 penton purification of soluble pentamers from Escherichia coli and development of an integrin-dependent gene delivery system.  Eur J Biochem. 2000 Oct;267(19):6074-81.
3. Bonnez W, Rose RC, Da Rin C, Borkhuis C, de Mesy Jensen KL, Reichman RC.  Propagation of human papillomavirus type 11 in human xenografts using the severe combined immunodeficiency (SCID) mouse and comparison to the nude mouse model.  Virology. 1993 Nov;197(1):455-8.

Pelleting Technique for Subcellular Fraction Electron Microscopy and/or Immunoelectron Microscopy

1. Beutner G, Sharma VK, Giovannucci DR, Yule DI, Sheu SS. Identification of a ryanodine receptor in rat heart mitochondria. J Biol Chem. 2001 Jun 15;276(24):21482-8. Epub 2001 Apr 10.
2. Pan X, Roberts P, Chen Y, Kvam E, Shulga N, Huang K, Lemmon S, Goldfarb DS.  Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p.  Mol Biol Cell. 2000 Jul;11(7):2445-57.

"Pop-Off" Technique for EM Examination of Cultured Cells

1. Roper JM, Mazzatti DJ, Watkins RH, Maniscalco WM, Keng PC, O'Reilly MA.  In vivo exposure to hyperoxia induces DNA damage in a population of alveolar type II epithelial cells.  Am J Physiol Lung Cell Mol Physiol. 2004 May;286(5):L1045-54. Epub 2004 Jan 16.

Color High Resolution (2.5 Um) Plastic Sections Utilizing Basic Fuchsin and Methylene Blue as a Hematoxylin & Eosin (H & E) Like Stain and Trichrome-Like Stain

1. di Sant'Agnese, PA and de Mesy Jensen, KL. Dibasic staining of large epoxy tissue sections and applications to surgical pathology. Amer. J. Clin. Pathol. 81:25, 1984.