Functional Genomics Center
Director
Stephen L. Welle, Ph.D.
Mission Statement
The purpose of the FGC is to provide support for the analysis of DNA and RNA, either by analyzing samples in the FGC laboratory or providing advice about laboratory protocols and data analysis.
Core Services
- DNA sequencing
- Genotyping
- Analysis of SNPs, microsatellite polymorphisms, or other DNA variations
- Affymetrix microarrays (gene expression, SNP, tiling)
- Preparation of labeled DNA or RNA for hybridization with microarray slides (any brand)
- Microarray hybridization, washing, and scanning
- Quantitative PCR (“real time”)
- Assistance with data analysis for all methods listed above
- DNA and RNA extraction from cells and tissues
- Measurement of DNA and RNA concentrations (UV absorbance)
- Assessment of RNA quality (Bioanalyzer)
- Reverse transcription of RNA
- Amplification of RNA and DNA (non-PCR)
- Applied Biosystems freezer program
- IDT oligonucleotide orders (saves shipping costs for individual labs)
- Advice about DNA and RNA analysis
- Access to liquid handling robots
Major equipment
- ABI 3730 DNA sequencer
- Affymetrix Fluidics Station 450, Scanner 3000, Hybridization Oven 640
- ABI 7900 HT real time PCR instrument with robotic plate loader and microfluidic card module
- Perkin Elmer ScanArray Lite microarray scanner (two color)
- Agilent 2100 Bioanalyzer
- Nanodrop ND-1000 spectrophotometers (including one at medical center for public use)
- Bio-Rad gel documentation system
- Tecan HS4800 Hybridization Station
- SciGene hybridization oven
- Perkin Elmer MiniTrak V liquid handling robot
- Perkin Elmer Multiprobe II HT EX liquid handling robot
- CAS 1200 liquid handling robot
Faculty Advisory Group
Six faculty members serve on the advisory group:
Dirk Bohmann
Nick Crispe
Beth Grayhack
Joe Miano
Steve Goldman
Charles Thornton
