Molecular Imaging FacilityDirectorScott Butler, Ph.D. Mission StatementThe Molecular Imaging Facility provides researchers at the University of Rochester with access to state of the art instruments capable of detecting and quantifying the levels and positions of radio- and fluorescently labeled molecules in a variety of formats including gels, blots and microtiter plates. Core ServicesThe facility provides users with access to two instruments designed to facilitate the analysis of samples containing radioactive- and fluorescent-labeled biomolecules. The virtue of the two systems is their sensitivity and digital storage, which allows easy quantitation and manipulation of images. InstrumentationStorm 820 Phosphorimager- Storage phosphorimaging allows extremely sensitive detection and digitized quantization of radiolabeled (32P, 14C, 35S, 125I and 3H) samples in gels, blots and microtiter plates. The virtues of this technology include the elimination of the need for film, high sensitivity, linearity over 5 orders of magnitude and computerized analysis and storage of data. The Storm 820 Phosphorimager is located in 3-7424.
Typhoon 9410 - The Typhoon 9410 is housed within the Center for RNA Biology. The instrument is a versatile system that handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and microarrays. Powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets. Red-, green-, and blue-excitation wavelengths and a wide choice of emission filters enable imaging of an extensive variety of fluorophores. Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increacing throughput, and saving time. Strorage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation. Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.
UsersThere is no formal user group. There are currently 41 Medical Center laboratories with permission to use this facility. |