URMC Pathology/Morphology Imaging Core (PMIC)
Confocal Imaging Capabilities
| Can do |
Currently Can't do |
| Green (Emission Wavelength Range: 500-550 nm) |
UV -no UV laser line, can substitute far red counterstains (ex. TO-PRO 3, Invitrogen, T3605) |
| Near Red (560-620 nm) |
|
| Far Red (640-720 nm) |
|
| GFP |
|
| YFP |
|
| CFP |
|
| all of the above with DIC (2 colors plus transmitted) |
|
| Laser lines: 458, 488, 543, and 633 nm |
|
| Technical Applications |
|
Optical sectioning with individual and stacked analysis
|
Live Imaging with environmental controls -can adapt with user environmental chambers |
| 3D analysis, 3D movie |
|
| Live Imaging (without environmental control) - with two Forma Scientific incubators next to microscope |
|
| FRET |
|
| Quantum Dot Analysis |
|
| Silicon Polymer Visualization |
|
| Material Penetration |
|
Leica Microscope Capabilities
| Upright Microscope |
Inverted Microscope |
| 5x dry |
10x dry |
| 20x oil, glycerol, water |
40x oil |
| 40x oil |
63x oil |
| 63x saline |
100x oil |
| 63x oil |
|
| 100x oil |
|
| 1.6x dry |
|
| 40x water |
|
| Rotatable Slide Holder |
|
| Fluorescent cubes |
Fluorescent cubes |
| Blue, and green excitation (green and red emission) |
Blue, and green excitation (green and red emission) |
|
