Department of Microbiology & Immunology: Resources

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Table 1:Xenopus Lymphocyte surface markers detectable with currently available mAbs

Xenopus markers (mAbs)	Expression pattern
CD8 (AM22, F17)	Larval and adult thymocytes (70-80%) and T-cells (about 20% of splenocytes). All lymphoid tumor lines
CTX (X71, 1S9.2)	Larval and adult thymocytes (60-70%)no consistent expression in periphery. All lymphoid tumor lines and gut epithelial tissue.
XT1 (XT1)	Most, but not all, larval and adult T-cells; earliest marker of thymocytes. All lymphoid tumor lines
MHC class I (TB17)	Ubiquitous in adult; all lymphopoietic lineages. Not expressed until metamorphosis
MHC class II (AM20, 14A2)	Thymocytes, B and T-cells (99% of spleen lymphocytes), only B-cells in larvae
CD5 (2B1)	Thymocytes (>95%), T-cells and some PMA-activated IgM+ B cells. All lymphoid tumor lines
NK-like (1F8)	Non-B and non-T, peripheral lymphoid cells.
RC47	Leukocyte lineage from very early stage. Thymic cortex and medulla (>90% of total thymocytes)
IgM (10A9)	Larval and adult B cells.
IgY (11D5)	Some larval and adult B cells.
IgX (410D9)	Some larval and adult B cells, especially in the gut.

Table 2: Some available cDNA encoding immunologically-relevant X. laevis genes

Immune System Characteristics	Larva (Ancestral-like system) Adult (Mammalian-like system)
CTX	Thymus, all lymphoid tumor lines and gut epithelial tissue.
TCR α, β, γ	thymus, circulating T-cells
BCR μ, γ, ξ, L	B cells
MHC class I	ubiquitous in adult
Non-classical class Ib	more restricted tissue distribution for some of them
MHC class II	thymus, splenic lymphocytes
Rag 1 & 2	mainly thymus, at lower level in bone marrow, kidney, spleen and liver
TdT	mainly thymus of old larvae and adult, less in spleen
Ikaros	mainly thymus
GATA III	thymus and T-cells
LMP7	Thymus, spleen
Hsps (gp96, hsp70)	Ubiquitousely expressed,

Table 3: Currently available X. laevis MHC-defined stains and clones

	Name, (MHC gentotype)
Partially inbred, MHC homozygous strains	F, J,
Isogenetic leavis/gilli (LG) clones with identical heterozygous (a/c) MHC genotype	LG-6, LG-7, LG-15
MHC-disparate LG isogenetic clones	LG-3 (b/d), LG-5 (b/c)

Table 4: Available cloned thymic tumor cell lines

Name of Tumor Cell Line	BB7	ff-2	15/0
Genetic background of initial tumor-bearing host	Partially inbred MHC homozygous ff strain		LG-15 (MHC a/c) isogenetic clone
T-cell surface markers (CD8, CD5, pan T-cell)	+++	+++	+++
Ig mRNA	-	+	+
Ig protein	-	-	-
MHC class I mRNA and protein	-	+	-
MHC class II mRNA and protein	-	-	-
Tumorigenicity in syngeneic
Larvae	-	+	++
Adult

List of available genomic and cDNA libraries