Ph.D.
(1975) |
Edith
Lord
Professor of Oncology in Microbiology & Immunology Primary Appointment: GEBS Cluster Affiliations: |
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| Contact Information: | ||
| University
of Rochester School of Medicine and Dentistry 601 Elmwood Ave, Box 672 Rochester, New York 14642 |
Medical Center KMRB 2-9617(Office) / 2-9812(Lab) Phone: (585) 275-5855 Fax: (585) 473-9573 E-Mail: edith_lord@urmc.rochester.edu |
|
- Research Focus
- Cell-Mediated Immunity to Tumors
- Research Overview
- We are interested in the immune responses which can control tumor development. We have been using a murine lung tumor model to study how different cytokines can affect the generation of specific cytolytic T cells. Transfection of the tumor cells with the genes for IL-2 or IL-3 was found to cause an increase in the number and function of cytolytic T cells present within the tumors. We are presently studying the molecular basis for this finding. IL-3 appears to increase the number and efficacy of a subpopulation of macrophages to effectively present tumor antigen in the context of class I major histocompatibility antigens to cytotoxic T cells. We have also found that IL-2 can also reverse the immunoinhibitory effects of another cytokine, transforming growth factor beta, that is produced by many tumor cells.
- We are also interested in studying other aspects of the unique microenvironment present within growing tumors. Of particular interest is the development of hypoxic areas that are deficient in oxygen due to the rapid growth of the tumor mass. We have made use of a new nitroimidazole compound called EF5, that is reduced under hypoxia and forms adducts with cellular protein. We have produced monoclonal antibodies that are reactive with this drug, and we are now able to detect hypoxic cells within tumors by immuno-cytochemistry, flow cytometric analysis and ELISA assays. We are currently studying how these hypoxic areas within tumors affect the expression of cell adhesion molecules that influence the infiltration by host immune cells.
- Recent Publications
- Santos K, Simon DA, Conway E, Bowers WJ, Mitra S, Foster TH, Lugade A, Lord EM, Federoff HJ, Dewhurst S, Frelinger JG. "Spatial and temporal expression of herpes simplex virus type 1 amplicon-encoded genes: implications for their use as immunization vectors." Hum Gene Ther. 2007 Feb;18(2):1-13.
- Ramanayake T, Simon DA, Frelinger JG, Lord EM, Robert J. "In vivo study of T-cell responses to skin alloantigens in Xenopus using a novel whole-mount immunohistology method." Transplantation. 2007 Jan 27;83(2):159-66.
- Gerber SA, Rybalko VY, Bigelow CE, Lugade AA, Foster TH, Frelinger JG, Lord EM "Preferential attachment of peritoneal tumor metastases to omental immune aggregates and possible role of a unique vascular microenvironment in metastatic survival and growth." Am J Pathol. 2006 Nov;169(5):1739-52
- Lugade AA, Moran JP, et al. "Local radiation therapy of B16 melanoma tumors increases the generation of tumor antigen-specific effector cells that traffic to the tumor." J Immunol. 2005 Jun 15;174(12):7516-23.
- Gerber, S. A., M. J. Turner, et al. "Characterization of a lymph node within the mouse prostate: Detailed analysis using whole mount histology." Prostate 63(2): 105-16, 2005.
- Moran JP, Gerber SA, Martin CA, Frelinger JG, Lord EM. "Transfection of the genes for interleukin-12 into the K1735 melanoma and the EMT6 mammary sarcoma murine cell lines reveals distinct mechanisms of antitumor activity." Int J Cancer. 106:690-8, 2003.
- Gerber SA, Moran JP, Frelinger JG, Frelinger JA, Fenton BM, Lord EM. "Mechanism of IL-12 mediated alterations in tumour blood vessel morphology: analysis using whole-tissue mounts." Br J Cancer 88:1453-61, 2003.
- Fisher TL, Nocera M, Willis RA, et al. "Generation of monoclonal antibodies specific for human kallikrein 2 (hK2) using hK2-expressing tumors." Prostate 51:153-65, 2002.
- Turner MJ, Abdul-Alim CS, Willis RA, Fisher TL, Lord EM, Frelinger JG. "T-cell antigen discovery (T-CAD) assay: a novel technique for identifying T cell epitopes." J Immunol Methods. 256:107-19, 2001.
- Lord E, Paoni S. "Effects of radiation on tumor intravascular oxygenation, vascular configuration, development of hypoxia, and clonogenic survival." Radiat Res. 155:360-8, 2001.
- Brown DM, Fisher TL, Wei C, Frelinger JG, Lord EM. Tumours can act as adjuvants for humoral immunity. Immunology 102:486-97, 2001.
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