1990 Cumulative Final Exam

Name______________________________

Box________________________________

Department of Pathology & Laboratory Medicine

General Pathology (Path. 500)

Final Examination

Monday, December l7, l990

9:00 a.m. - 12:00 noon

INSTRUCTIONS: (1) Write your name and box number on each page.

(2) Read each question carefully. How it is asked could

be as important as what is asked.

(3) Suggested time allotments:

Part I: 1 hour

Parts II and III: 2 hours

Name_____________________________

Box_______________________________

Part III

Write the letter preceding the ONE best answer in the space provided next to each question.

_______l. a. In comparison with young people, the bodies of older people

have more water and less fat.

b. The lipid peroxidation theory of aging is better supported by

experimental and clinical data than any other theory of aging.

c. Chemicals such as acetaminophen or bromobenzene produce

lipid peroxidation by means of activated oxygen species

formed by metabolism in the mixed-function oxidase system.

d. The brain is not subject to dystrophic calcification.

e. Although heavy metals comprise a heterogeneous group, their

cytotoxic effects are all mediated by the hydroxyl radical.

_______2. a. The accumulation of water in the cytoplasm in hydropic swell-

ing compresses the cisternae of the rough endoplasmic

reticulum, which therefore appear as thin, bilayered profiles

densely studded with ribosomes.

b. The separation of the fibrillar from the granular component

of the nucleolus is a sign of irreversible cell injury.

c. Atrophic cells have reduced energy requirements.

d. In adapting to injury, cells lose water and, therefore, a

reversibly injured cell is typically shrunken and smaller

than normal.

e. In contrast to cells in coagulative necrosis, cells in caseous

necrosis retain their outlines.

Name_____________________

Box______________________

Write the letter preceding the ONE best answer in the space provided next to each question.

_______3. Epithelioid cells in tubercles are derived from....

a. fibroblasts

b. lymphoblasts

c. alveolar epithelial cells

d. macrophages or monocytes

e. mesothelial cells

_______4. If a mammalian cell is in G1 phase....

a. it must undergo mitosis in order to survive

b. it generally will undergo mitosis within two hours

c. its RNA and protein content are, as a rule, rising in less

than three hours

d. it must enter S phase promptly

e. it ceases to glycosylate proteins

_______5. Homogeneous T-cell receptor gene rearrangements in lymphoma cells from a patient with malignant lymphoma indicate....

a. phenotypic identity

b. T-suppressor cell function

c. T-helper cell function

d. cytotoxic function of cells

e. monoclonal origin

_______6. The neoplastic cells in rapidly growing cancers....

a. are, as a rule, highly differentiated

b. often skip G1 phase when replicating

c. vary in rate of replication

d. are incapable of glycolysis

e. are likely to be highly synchronized for cell division

Name_________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

_______7. Which of the following collagen types is found exclusively in basement membranes?

a. Type I

b. Type II

c. Type III

d. Type IV

e. Type V

_______8. Which one of the following is not true for proteoglycans?

a. They contain amino sugars.

b. They are negatively charged molecules.

c. They are highly hydrophilic.

d. Their distribution is tissue-specific.

e. They appear in healing wounds during late stages of scar

formation.

_______9. A healing wound reaches its maximal tensile strength....

a. at 2 weeks

b. at 6 weeks

c. at 3 months

d. at l year

e. over an unpredictable period of time

______l0. Which ONE of the following statements is TRUE?

a. Cancer cells always proliferate faster than their normal

counterparts.

b. Polypeptide growth factors implicated in neoplasia do not

act on normal cells.

c. Malignant tumors are invariably composed of homogeneous cell populations that are of monoclonal origin.

d. Spleen and skeletal muscle are frequently involved by

metastases of epithelial tumors because of their size and abundant blood supply.

e. RNA retroviruses may cause cancer either by integrating their oncogene into the cellular genome or by the activation

of pre-existing cellular proto-oncogenes.

Name__________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

______11. The progressive and rapid growth of many neoplasms is an indication of....

a. marked shortening of the cell replication times as compared to normal cells

b. marked shortening of S phase

c. absence of G2 phase

d. number of completed cell replication cycles per unit of

time (e.g. month)

e. inability of host to mount immune responses

______12. The replicative pools of cells in a cancer are generally....

a. the most differentiated cancer cells

b. synchronized for replication by host factors

c. a numerical majority of the cancer cells

d. a numerical minority of the cancer cells

e. synchronized once they have metastasized

______13. Many sorts of dividing cancer cells are genetically unstable and undergo mutations that influence the subsequent clinical

behavior of cell sub-populations, usually in the direction of

greater malignancy. Such behavior of cancer cells is termed....

a. initiation

b. promotion

c. aggression

d. progression

e. resilience

Name_________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

______14. A biopsy specimen from a uterine ectocervix was reported as

showing squamous cell carcinoma in situ. Which one of the

following would be out of line with that diagnosis?

a. loss of normal epithelial stratification

b. presence of bipolar mitoses

c. presence of anaplastic cells in the cervical epithelial cover

d. presence of anaplastic epithelial cells in the subepithelial

stroma

e. loss of normal polarity of cells in the cervical epithelial cover

______15. When cancer cells are grown in vitro, they generally display

behavior not associated with non-neoplastic cells growing

under similar circumstances. Which one of the following is

characteristic only of cancer cells?

a. absence of autophagy in cultured cells

b. confluent growth

c. loss of contact inhibition

d. altered shape of cells

e. altered size of cells

______l6. Which ONE of the following statements is FALSE about initiation

of cancer?

a. Most initiated cells never display neoplastic behavior.

b. Initiation precedes promotion.

c. Initiation confers growth autonomy upon cells.

d. Initiation of cells can result from point mutations.

e. Initiation of cells can result from frameshift mutations.

Name__________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

______17. Which one of the following statements about carcinogenic

chemicals is FALSE?

a. Many are both initiators and promoters.

b. Some require no metabolic activation or conversion for

carcinogenicity.

c. Most are mutagens.

d. Some are metals.

e. They are generally not synergistic.

______18. Viewed statistically, the most significant factor in effecting cure

of any cancer is....

a. accurate staging

b. accurate grading

c. use of fine needle aspiration

d. early detection and diagnosis by whatever means

e. extensive screening of populations at risk

______19. Which one of the following statements is true?

a. Squamous metaplasia of the bronchial epithelium induced by

cigarette smoke is irreversible and, if untreated, progresses

to cancer.

b. Squamous metaplasia, in which one type of epithelium is con-

verted to another, is invariably beneficial, because it provides

the tissue with additional protection against adverse external

influences.

c. The term "dysplasia" denotes cytologic changes, primarily

reflected in the morphology of the nuclei, but does not include changes in the arrangement of cells within the tissue.

d. Endometrial thickening caused by the administration of

exogenous hormones is an example of hyperplasia.

e. The term "dysplasia" is applied to many different conditions

in all of which there is an increased risk of cancer.

Name__________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

20 - 23 - Match each of the following carcinogenic chemicals (A) with one

statement under (B).

A B

_____20. Alkylating agents a. Their relation to nitrite food

additives has raised many

questions.

_____21. Polycyclic hydrocarbons b. Have pre-eminently served to

clarify many aspects of liver

cancer.

_____22. Azo Dyes c. Major atmospheric pollutants

_____23. Nitrosamines d. Used in treating cancer

24 - 28 - Complete the following table of corresponding benign and

malignant neoplasms.

Neoplasm

BENIGN MALIGNANT

24. Hemangioma________________________________________

25. Chondrosarcoma

26. Leiomyoma__________________________________________

27. Epidermoid carcinoma

28. Cystadenoma________________________________________

Name________________________

Box__________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

______29. Which one of the following statements is true of inositol

triphosphate?

a. It stimulates protein kinase C activity by stimulating the

release of calcium from the endoplasmis reticulum.

b. Phosphodiesterase converts it back to inositol 1, 4, 5-tri-

phosphate.

c. Its production is stimulated by a cascade of phosphorylation

events.

d. It is active when associated with the plasma membrane.

______30. Which one of the following statements is true of oncogene

products in the cell nucleus?

a. They exert their effect through their DNA binding activities.

b. They are typically non-functional as proto-oncogenes.

c. They are synthesized in the nucleus and act in the cytoplasm.

d. They stimulate phosphorylation of the RB protein.

e. They must be activated by chromosomal rearrangement in

order to function.

______31. Which statement best describes anti-oncogene function?

a. They are normal cellular proteins which are activated by

the overexpression of deregulation of an oncogene.

b. They are cellular proteins which function in normal cellular

regulation.

c. Through direct binding with an oncogene protein they re-

store normal cellular functions.

d. Most cells lack these functions.

Name_________________________

Box___________________________

Write the letter preceding the ONE best answer in the space provided next to each question.

______32. Which of the following is NOT a form of antigen-specific

immunotherapy?

a. monoclonal antibodies carrying a toxin

b. interferon immunotherapy

c. adoptive immunotherapy

d. tumor cell vaccines

______33. Lymphocytes kill tumor cells by which one of the following

mechanisms?

a. adhesion of surfaces, cytokine transfer

b. cytokine release, membrane rupture

c. adhesion of surfaces, toxic granule transfer

d. cytokine release, phagocytosis

______34. Which of the following characteristics of tumor cells is critical

for active specific immunotherapy?

a. hyperdiploid (greater than 46 chromosomes)

b. production of IL-1 and/or IL-2

c. presence of HLA class III molecules

d. expression of tumor associated antigens

Name_________________________

Box___________________________

ANSWER: A if only item 1 is correct

B if only items 1 and 3 are correct

C if only item 2 is correct

D if only item 3 is correct

E if only items 2 and 4 are correct

F if all four (1, 2, 3, 4) items are correct

______35. Which of the following factors have a role in clearance of

particles from the broncho-alveolar airways?

1. mucociliary "escalator"

2. cough reflex

3. rate of respiration

4. macrophages

______36. Coalworkers' pneumoconiosis may, if complicated, give rise to....

1. large (> 2 cm) black scars in the lungs

2. cavitation of lungs

3. "rheumatoid" lesions in the lungs

4. cor pulmonale

______37. The most frequent hallmarks of pulmonary asbestosis are....

1. patchy alveolar fibrosis in periphery of upper lobes

2. diffuse interstitial fibrosis in the periphery of lower lobes

3. presence of uncoated chrysotile fibers in alveolar air spaces

4. ferruginous bodies with cores of asbestos in areas of diffuse

interstitial fibrosis

______38. Silicosis of the lungs....

1. carries an increased risk of lung cancer

2. carries an increased risk of pulmonary tuberculosis

3. carries an increased risk of cancer of the larynx

4. may be complicated by the development of Caplan's

syndrome

Name_________________________

Box___________________________

ANSWER: A if only item 1 is correct

B if only items 1 and 3 are correct

C if only item 2 is correct

D if only item 3 is correct

E if only items 2 and 4 are correct

F if all four (1, 2, 3, 4) items are correct

______39. The use of cocaine or its derivative "crack" in relatively high

doses often leads to....

1. ventricular arrhythmias

2. necrosis of cardiac myocytes

3. contraction bands in cardiac myocytes

4. pulmonary edema

40 - 44 - Write the ONE best answer in the space provided next to each question.

______40. In a contact sensitivity reaction to poison ivy (rhus), what

happens first?

a. monocytes become activated by direct contact with rhus oils

b. natural killer cells respond to antigen-antibody complexes

localized to the skin

c. T-cells bearing antigen receptors specific for rhus antigens

are activated by contact with antigen

d. blood monocytes are recruited to the skin by cytokines

released by T-cells

______41. In which of the types of hypersensitivy reaction is the target

cell actually destroyed directly by a lymphocyte?

a. Type I (anaphylactic)

b. Type II (cytotoxic)

c. Type III (immune complex)

d. Type IV (cell-mediated)

e. none of the above

Name_________________________

Box___________________________

40 - 44 - Write the ONE best answer in the space provided next to each

question.

______42. In the formation of a granuloma as part of a Type IV (cell-

mediated) immune response, which is the first event to occur

in the usual sequence of events?

a. recognition of antigen by specific T-cell clones

b. activation of monocytes to form "epithelioid cells"

c. formation of giant cells

d. recruitment of monocytes by T-cells

______43. Anti-nuclear antibodies....

1. are specific for SLE (systemic lupus)

2. are never found in normal individuals

3. bind to unknown antigens in the nucleus

4. are a broad group of antibodies reacting to various mostly

known nuclear structures, a fact which is of practical

diagnostic usefulness in certain diseases

______44. Serum sickness usually occurs 7 - l0 days after injection of the

foreign protein because....

1. T-cells require that much time to recruit monocytes to site

of antigenic contact

2. neutrophils and monocytes require at least 1 week to respond

to deposition of complement in vessels and other tissues

3. of the necessity to generate cytotoxic T-cells

4. the disease develops after formation of antibody to the foreign

protein, which requires this long for a primary response

Name_________________________

Box___________________________

Part I

INSTRUCTIONS: Use only the space provided on this page for your answer. Nothing on the backs will be graded. Answers must be in ink (pen). Illegible answers will not be graded.

1. An apparently healthy 17-year old male begins to have repeated

episodes of pulmonary thromboembolism. Briefly, outline a general plan by which you would investigate the patient's problem.

Name________________________

Box__________________________

INSTRUCTIONS: Use only the space provided on this page for your answer. Nothing on the backs will be graded. Answers must be in ink (pen). Illegible answers will not be graded.

2. Compare and contrast the gross and microscopic appearance of necrosis in a non-tuberculous bacterial abscess and in tuberculous caseation in lung.

A. Non-tuberculous:

B. Tuberculous:

C. Briefly, differentiate the pathogenesis of the two forms of necrosis.

Name_________________________

Box___________________________

INSTRUCTIONS: Use only the space provided on the this page for your answer. Nothing on the backs will be graded. Answers must be in ink (pen). Illegible answers will not be graded.

3. A 22-year old woman with leukemia was given total body irradiation of 1000 rad in divided doses in preparation for bone marrow transplantation from her mother, performed one week after the last dose of radiation. Two days later, despite anti- biotics, the patient developed overwhelming sepsis with blood cultures positive for fungi. She died 5 days after marrow transplantation.

A. Briefly, describe microscopic changes you would find at autopsy in bone marrow, spleen, and lymph nodes.

B. Briefly, describe possible microscopic, pathological changes in parenchymal organs such as heart, lungs and kidneys.

Name_________________________

Box___________________________

Part II

Answer: A if items 1, 2, 3, are correct

B if items 1 and 3 are correct

C if items 2 and 4 are correct

D if item 4 is correct

E if all four (1, 2, 3, 4) items are correct

_______1. The body's main response(s) to an obligate extracellular

pathogen include:

1. Acute exudative response

2. Granulomatous response if the pathogen is very large

3. Antibody-mediated immune response

4. Cell-mediated immune response

_______2. Pathogens which are typically spread through the

mother's blood to the placenta and fetus include:

l. Toxoplasma gondii

2. Group B beta-hemolytic Streptococci

3. Cytomegalovirus

4. Gardnerella vaginalis

_______3. Which of the following are examples of virulence

factors?

1. Meningococcal exotoxin

2. Staphylococcal coagulase

3. Pneumococcal exotoxin

4. Mycobacterial cording factor

Name_________________________

Box___________________________

Answer: A if items 1, 2, 3 are correct

B if items 1 and 3 are correct

C if items 2 and 4 are correct

D if item 4 is correct

E if all four (1, 2, 3, 4) items are correct

_______4. Route(s) of body invasion which occur(s) with viruses,

protozoa, and bacteria, but not with fungi include:

1. Transcutaneous

2. Respiratory

3. Gastrointestinal

4. Transplacental

_______5. Lyme disease is caused by a spirochete (Borrelia burg-

dorferi) spread by an insect vector. Since it is caused by

a spirochete, one would expect the disease to have:

1. A primary lesion at the inoculation site

2. A secondary phase with symptoms related to systemic

spread

3. Stillbirths caused by maternal infection

4. A tertiary phase long after the initial infection

_______6. Opportunistic infections are....

1. Caused by organisms which are not normally invasive

2. Infrequent in a hospitalized population

3. Vary according to the type of abnormal defenses

4. Only knock once

_______7. Corynebacterium diphtheriae....

1. Causes a tracheal inflammatory pseudomembrane

2. Produces toxin only when infected by a bacteriophage

3. Toxin causes myocarditis and neuritis

4. Causes tracheal perforation

Name_________________________

Box___________________________

Answer: A if items 1, 2, 3, are correct

B if items 1 and 3 are correct

C if items 2 and 4 are correct

D if item 4 is correct

E if all four (1, 2, 3, 4) items are correct

_______8. In syphilis....

1. Neutrophilic and eosinophilic inflammation are

characteristic

2. The causative organism can't be cultured in vitro.

3. Granulomas are part of the secondary stage

4. Production of antibodies is effective against re-

infection

_______9. Members of the genus Clostridium produce....

1. Enterotoxins

2. Hemolysins

3. Neurotoxins

4. Endotoxins

______10. Coccidioidomycosis....

1. Causes infiltration by neutrophils

2. Does not cause granulomatous inflammation

3. May mimic tuberculosis in x-ray images

4. Is primarily an enteric infection

Name_________________________

Box___________________________

Answer: A if items 1, 2, 3, are correct

B if items 1 and 3 are correct

C if items 2 and 4 are correct

D if item 4 is correct

E if all four (1, 2, 3, 4) items are correct

______11. Miliary tuberculosis....

1. Is characterized by granulomas without necrosis

2. Can be seen in many parts of the body

3. Is widely disseminated throughout the lungs

4. Is named for the high prevalence in army camps

______12. Patients with A.I.D.S. tend to have infections by....

1. Pyogenic bacteria

2. Herpes group viruses

3. Enteric bacteria

4. Enteric protozoa

______13. Parts coded in a virus's genome include....

1. Capsid

2. Nucleic acid polymerase

3. Envelope proteins

4. Envelope lipids

______14. Mechanisms by which organisms avoid or survive

phagocytosis include....

1. Enzymes to produce peroxide

2. Carbohydrate-rich capsules

3. Hemagglutinins

4. Waxy cell walls

Name_________________________

Box___________________________

Answer: A if items 1, 2, 3, are correct

B if items 1 and 3 are correct

C if items 2 and 4 are correct

D if item 4 is correct

E if all four (1, 2, 3, 4) items are correct

______15. Lobar pneumonia is caused by all of the following

except:

1. Legionella pneumophila

2. Streptococcus pneumoniae

3. Klebsiella pneumoniae

4. Bordetella pertussis

______16. Entamoeba histolytica can....

1. Have an asymptomatic carrier state

2. Invade the liver via portal vessels

3. Phagocytize red blood cells

4. Cause liquefactive necrosis of bowel mucosa

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