Approved Euthanasia Dosage and Techniques
(Table 2)

RODENTS

1. Sodium Pentobarbital 100 mg/kg IV or IP

2. Carbon Dioxide Inhalation Chamber followed by secondary physical method (i.e. pneumothorax, cervical dislocation for rodents under 200 grams, decapitation, perfusion of a histological fixative via the major blood vessels or complete severing of the spine just below the base of the skull using a dorsal approach)

3. Cervical dislocation for rats weighing less than 200 grams and all mice after sedation (unless otherwise scientifically justified to U.C.A.R.)

4. Decapitation with guillotine only after the animal has been sedated (unless otherwise justified to U.C.A.R.)

5. Cardiac perfusion or exsanguination under deep plane of surgical anesthesia.

RABBITS, NONHUMAN PRIMATES, DOGS, CATS, SWINE

1. Sodium Pentobarbital 100 mg/kg IV

2. Cardiac perfusion or exsanguination under deep plane of surgical anesthesia.