Paul Daniel Kingsley, Ph.D.

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Dr. Kingsley's research interests are:

1) Embryonic Hematopoiesis

2) Erythroid Differentiation

3) Ontogeny of Hematopoietic Progenitors

4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Specialty

Hematology / Oncology

Current Appointments

• Research Assistant Professor - Department of Pediatrics (SMD)
• Research Assistant Professor - Center for Pediatric Biomedical Research (SMD)

Education
PhD Developmental Biology	University of Rochester	1992
MS Developmental Biology	University of Rochester	1987
BA Biology	Reed College	1982

Post-Doctoral Training & Residency
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY	1992 - 1994

Fellowship Awards
Postdoctoral Traineeship, National Cancer Institute, University of Rochester, Rochester, NY	1992 - 1993

Recent Journal Articles
Showing the 5 most recent journal articles. (36 available)
Naik, A.A.; Xie, C.; Zuscik, M.J.; Kingsley, P., Schwarz, E.M.; Awad, H.; Guldberg, R.; Drissi, H.; Puzas, J.E.; Boyce, B.; Zhang, X; O'Keefe, R.J. "Reduced COX-2 expression in aged mice is associated with impaired fracture healing." J Bone Miner Res. 24 (2009): 251-264.
McGrath KE; Kingsley PD; Koniski AD; Porter RL; Bushnell TP; Palis J. "Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus." Blood. 2008; 111(4):2409-17. Epub 2007 Nov 21.
Keel SB; Doty RT; Yang Z; Quigley JG; Chen J; Knoblaugh S; Kingsley PD; De Domenico I; Vaughn MB; Kaplan J; Palis J; Abkowitz JL. "A heme export protein is required for red blood cell differentiation and iron homeostasis." Science (New York, N.Y.). 2008; 319(5864):825-8.
Bullard T; Koek L; Roztocil E; Kingsley PD; Mirels L; Ovitt CE. "Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands." Developmental biology. 2008; 320(1):72-8. Epub 2008 Apr 23.
Kingsley PD; Malik J; Emerson RL; Bushnell TP; McGrath KE; Bloedorn LA; Bulger M; Palis J. ""Maturational" globin switching in primary primitive erythroid cells." Blood. 2006; 107(4):1665-72. Epub 2005 Nov 01.