Gloria Salvini Pryhuber, M.D.

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Dr. Pryhhuber's research interests are:

1) Inflammatory Lung Disease

2) Interstitial Fibrosis

3) Silicosis

4) Bronchopulmonary Dysplasia.

Up to 300,000 adults and infants per year in the U.S. suffer from acute inflammatory lung diseases such as pneumonia, acute respiratory distress syndrome (ARDS) and respiratory distress of prematurity, while many times that number suffer from chronic, fibrotic lung disease. The proinflammatory cytokine, tumor necrosis factor-a (TNF), is a major pathogenic factor in these acute and chronic lung diseases. Recent discoveries advanced understanding of TNF signaling mechanisms that account for the highly pleiotropic and cell type specific effects of the cytokine. Dr. Pryhuber's laboratory is applying these advances in molecular biology to study how TNF and oxidant stress interact to alter pulmonary cell survival, gene expression and function, with an ultimate goal of improving therapy for inflammatory lung disease.

In her laboratory, cell culture and whole animal models are being utilized to determine signaling mechanisms by which members of the NGF/TNF receptor superfamily, of which TNF receptor I and II (TNFRI and TNFRII) are representative, regulate gene expression, inflammation and apoptosis in the lung. They recently demonstrated marked induction of TNF receptor associated factors (TRAF) and Inhibitor of Apoptosis Proteins (IAPs) by TNF in pulmonary epithelial cells in vitro, in mouse lung following intratracheal TNF treatment and in inflamed human and baboon lung tissue. They are pursing studies to establish cellular and developmental localization, regulation and function of the TRAF-1 and cIAP proteins in lung, as well as their role in TNF-induced proliferation, programmed cell death and transcription factor AP-1 and NFkB activation. Effects of TNF on pulmonary cell function are also being studied in whole animal models. Structural and biochemical abnormalities are measured following exposure of "knock-out" transgenic mice, in which gene expression of either of the TNF receptors or of a signal transduction intermediate protein, such as TRAF1, is prevented, to intratracheal administration of TNF, silica or hyperoxia, in order to understand mechanisms of TNF mediated injury and repair.

Specialty

Neonatology

Current Appointments

• Associate Professor - Department of Pediatrics (SMD)
• Associate Professor - Department of Environmental Medicine (SMD)