Animal Husbandry Information Animal Water Quality The NIH Guide (ILAR 1996) recommends that laboratory animals should have access to potable, uncontaminated drinking water. Water quality testing of the Rochester water supply includes periodic monitoring for pH, hardness, and microbial or chemical contamination. The most recent report from the Monroe County Water Authority indicates that Rochester drinking water meets all New York State and USEPA drinking water standards. Please refer to the website link above for more details about detected contaminants and a water quality summary. Supplemental treatment (e.g., autoclaving, acidification, hyperchlorination) of water provided to the University's laboratory animals is available by special request. All water provided to animals housed in the KMRB and CVRI facilities is treated by reverse osmosis. Water Delivery Systems HYDROPAC® Close monitoring of newly weaned pups on HYDROPAC®: The pink flag is designated for PI use to label newly weaned mice. This will assist the vivarium staff in identifying cages where animals might have a problem accessing water. The PI writes the date weaned on the pink flag and affixes it to the cage card of the newly weaned pups. The PI places several pieces of rodent diet on the cage floor and moistens them with water from Hydropac®. This action serves two purposes: providing moistened chow that can be easily accessed by pups and “flicking” the Hydropac® valve. Vivarium staff will pay extra attention to pink flagged cages for any indication that the mice are not eating or drinking. This intense monitoring will continue daily for a two week period after weaning. Any mice that do not appear well will be reported to the DCM veterinary staff. The PI will be contacted by DCM regarding the problem and recommended treatment. If the weanlings appear healthy after two weeks, the animal care staff will remove the pink flag. Rodent Diet and Bedding The standard laboratory rodent diet fed to our mice and rats and chemical compositions are: Laboratory Rodent Diet 5001 Standard Rooms Laboratory Autoclavable Diet 5010 MIT Rooms The standard bedding used with our rodents is: 1/8" Performance Bedding All Rooms Primate Diet and Bedding The standard laboratory diet fed to our primates and chemical compositions are: High Protein Monkey Diet 5045 Old World Primates New World Primate Diet 5040 New World Primates The standard bedding used with our primates is: Pel-o' Cobs® Rabbit Diet and Bedding The standard laboratory diet fed to our rabbits and chemical compositions are: Laboratory Rabbit Diet HF 5326 The standard bedding used with our rabbits is: Pel-o' Cobs® Animal Room Temperatures & Humidity Ranges All of the University of Rochester's animal holding rooms are maintained within temperature and humidity ranges described in the ILAR Guide for the Care and Use of Laboratory Animals (1996) with very rare exceptions. The building HVAC systems occasionally fail to tightly control room humidity in the face of outdoor seasonal extremes (Winter levels < 30%, Summer levels >70%). Please note that the animal room humidity levels rarely fall outside of range (less than 10 days in the Winter and Summer) and that the change in humidity is gradual over time. Be aware that we cannot control these outliers. Animal Room Temperature Ranges Mouse, rat, hamster, gerbil, guinea pig: 64-79F Rabbit: 61-72F Cat, dog, non-human primate: 64-84F Farm animals and poultry: 61-81F Animal Room Relative Humidity Range: 30-70% Recommendations for Husbandry of Severely Immunocompromised Mice The Animal Resource recommends the following special diet and water for severely immunocompromised mice such as NOG, SCID, RAG-2, Athymic Nude, and Beige mice. Immunodeficient mice also require strict attention to MicroIsolator Technology. The following practices are available to investigators by submission of a special request to the Animal Resource Office: Autoclaved acid water (pH 2.5-3.0) in sterile water bottles changed once weekly. This prevents a biofilm or overgrowth of Pseudomonas sp. (and other bacteria) in the water bottle. Feed irradiated TEST DIET MODIFIED ISOPRO (0.124% Sulfadiazine/0.025% Trimethoprim—5TVP mouse chow. The vivarium has this diet available on inventory. Please be sure to incorporate this information into your UCAR protocol as a Vivarium recommended husbandry practice for severely immunocompromised mice.