Instruments

Laser Scanning Confocal Microscope I (FV1000 Olympus)

FV1000Microscope of choice for the following studies:

  • Slides with fluorescent labels on cultured cells or tissue sections which require high resolution imaging
  • Samples up to 100 um thicknesses
  • Live imaging studies both short- and long-term including FRET, FRAP, FLIP, Photobleaching
  • Multiple time point ("mark and find" studies) and studies requiring image tiling
  • All fluorophores within the visible range with excitations beginning at 405nm can be imaged with this spectral laser confocal system. See below for laser lines.

Summary of Instrument Features

  • Microscope: Olympus IX81 (INVERTED microscope configuration i.e. objectives below the sample)
  • Objectives:
    • Normal configuration: 4x (N.A. 0.16),, 10x (N.A. 0.40), 20x oil (N.A. 0.85), 40x oil (N.A. 1.30), 60x oil (N.A. 1.42), 100x oil (N.A. 1.40); Dry objectives available: 60x water .
    • Dry objectives available:  20x dry, (N.A. 0.75), 40x dry (N.A. 0.60),  60x dry (N.A.   0.70)
    • Water objectives available:   60x water (N.A. 1.20 (double check)
  • DIC Capabilities: 20x, 40x, 60x, 100x
  • Lasers: 405 diode laser, 440 diode laser, 488/515 argon laser, 559 diode laser, 635 diode laser
  • Stage: Prior, Inc. motorized stage, includes tiling and multi-point mark and find memory function
  • Stage Holders: Multiple stage holder formats are provided by the Core. Current stage holders will support: 1 x 3 slides, 2 x 3 slides, circular (all sizes) glass-bottom culture dishes, well plates with glass-coverslip bottoms
  • Environmental Chamber: FULL STAGE INCUBATOR on the microscope, with CO2, temperature, and humidity regulation. Short- or long-term live imaging studies including FRET, FRAP, Multiple point timelapse imaging, etc. are possible
  • New Binder Incubator in room: Cultures can be equilibrated for a number of hours before being transferred into the stage incubator with identical C02, temp. and humidity conditions.
  • Service Contract Support: The FV1000 is under full service contract by Olympus.
  • Opportunities to work directly with Olympus reps: The core organizes multiple opportunities for PIs and staff to talk or work directly with the Olympus rep when they are on-campus.

 

Conventional Microscope (Olympus VanoxAH-2)

Vanox AH-2Microscope of choice for the following study types:

  • Studies needing brightfield microscopy
  • Samples with strong fluorophore signal needing standard conventional fluorescence imaging
  • Studies requiring "Live counting" image analysis

Summary of Instrument Features

  • Microscope: Upright microscope configuration (i.e. objectives above the sample)
  • Objectives: 4x, 10x, 20x, 40x, 100x
  • Lamp source: Mercury Lamp
  • Stage: Prior, Inc. Motorized Stage
  • Rotatable slide holder: full rotation capabilities
  • Camera: MicroFire Color Camera
  • Image capture software: PictureFrame
  • Image analysis software: StereoInvestigator (version 8.0)
    • SI modules currently in Core:
      • Optical Fractionator
      • Confocal quantitation module
      • Space Balls module

Laser Capture Microdissection Microscope (Zeiss Palmbeam)

NEW!! NOW AVAILABLE FOR USE!

Zeiss Palmbeam Laser Capture

Microscope of choice for the following study types:

* Capture of groups of phenotypically similar cells from fresh frozen, fixed then frozen, FFPE tissue onto eppendorf tube caps for further analysis  by  DNA, RNA, and protein assays

* Capture of cultured cells for further expansion or downstream processing for DNA, RNA, and protein analyses

Summary of Instrument Features

* Microscope:  Upright microscope configuration (i.e. objectives above sample)

* Objectives:  10x, 20x, 40x, 63x  dry  - long working distances (able to see through back of slides with good resolution)

* LED fluorescence lightsource:  Xcite Lamp

* Capture laser:  Pulsed UV laser (100 nanosecond pulses)

* Cap Holder:  Holds two caps

* Sample holders:

            a)_for 1x 3 slides – holds 3 slides at a time

            b)  Culture holders

* Cameras: two cameras:

            a) CC1 color camera

            b) mRM camera for fluorescence

{Note:  Both cameras provide the ability to capture “before cut, after cut, and on-cap” images}

* Stage:  motorized stage

 

High Definition Camera with Adjustable Photography Stand for Large Specimen Imaging

Large Specimen High Def Camera

Imaging equipment of choice for:

  • Studies requiring comparison of histologically processed sections to the original thick slice of tissue and original organ (i.e. comparative microscopy for gross organ, macro slices to paraffin-embedded histology samples).

Summary of Instrument Features

  • Stand: Kodak adjustable photography stand
  • Camera: SPOT Camera
  • Software: Image Pro Plus

Image Analysis Capabilities

At the current time, the following image analysis software packages are offered through the Core:

  • StereoInvestigator for quantitation of counts of cells using the Optical Fractionator method, version 8.0
  • Image Pro Plus
  • FV1000 Software - basic analysis of confocal images

Contact Us

Director:
Linda Callahan

Phone: 275-1317

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more to come!