Bone Marrow Transplantation
What is a bone marrow transplant?
Bone marrow transplant (BMT) is a special therapy for patients with certain cancers
or other diseases. A bone marrow transplant involves taking cells that are normally
found in the bone marrow (stem cells), filtering those cells, and giving them back
either to the donor (patient) or to another person. The goal of BMT is to transfuse
healthy bone marrow cells into a person after his or her own unhealthy bone marrow
has been treated to kill the abnormal cells.
Bone marrow transplant has been used successfully to treat diseases such as leukemias,
lymphomas, aplastic anemia, immune deficiency disorders, and some solid tumor cancers
What is bone marrow?
Bone marrow is the soft, spongy tissue found inside bones. It is where most of the
body's blood cells develop and are stored.
The blood cells that make other blood cells are called stem cells. The most primitive of the stem cells is called the pluripotent stem cell. This is different than other blood cells with regard to the following properties:
It is the stem cells that are needed in bone marrow transplant.
Why is a bone marrow transplant needed?
The goal of a bone marrow transplant is to cure many diseases and types of cancer.
When the doses of chemotherapy or radiation needed to cure a cancer are so high that a
person's bone marrow stem cells will be permanently damaged or destroyed by the treatment,
a bone marrow transplant may be needed. Bone marrow transplants may also be needed
if the bone marrow has been destroyed by a disease.
A bone marrow transplant can be used to:
Replace diseased, nonfunctioning bone marrow with healthy functioning bone marrow
(for conditions such as leukemia, aplastic anemia, and sickle cell anemia).
Regenerate a new immune system that will fight existing or residual leukemia or other
cancers not killed by the chemotherapy or radiation used in the transplant.
Replace the bone marrow and restore its normal function after high doses of chemotherapy
and/or radiation are given to treat a malignancy. This process is often called rescue.
Replace bone marrow with genetically healthy functioning bone marrow to prevent more
damage from a genetic disease process (such as Hurler's syndrome and adrenoleukodystrophy).
The risks and benefits must be weighed in a thorough discussion with your healthcare
provider and specialists in bone marrow transplants before the procedure.
What are some diseases that may benefit from bone marrow transplant?
The following diseases are the ones that most commonly benefit from bone marrow transplant:
However, patients experience diseases differently, and bone marrow transplant may
not be right for everyone who suffers from these diseases.
What are the different types of bone marrow transplants?
There are different types of bone marrow transplants depending on who the donor is.
The different types of BMT include the following:
Autologous bone marrow transplant. The donor is the patient himself or herself. Stem cells are taken from the patient
either by bone marrow harvest or apheresis (a process of collecting peripheral blood
stem cells), frozen, and then given back to the patient after intensive treatment.
Often the term rescue is used instead of transplant.
Allogeneic bone marrow transplant. The donor shares the same genetic type as the patient. Stem cells are taken either
by bone marrow harvest or apheresis from a genetically matched donor, usually a brother
or sister. Other donors for allogeneic bone marrow transplants may include the following:
A parent. A haploid-identical match is when the donor is a parent and the genetic match is at
least half identical to the recipient. These transplants are rare.
Unrelated bone marrow transplants (UBMT or MUD for matched unrelated donor). The genetically matched marrow or stem cells are from an unrelated donor. Unrelated
donors are found through national bone marrow registries.
Umbilical cord blood transplant. Stem cells are taken from an umbilical cord immediately after delivery of an infant.
These stem cells reproduce into mature, functioning blood cells quicker and more effectively
than do stem cells taken from the bone marrow of another child or adult. The stem
cells are tested, typed, counted, and frozen until they are needed for a transplant.
How are a donor and recipient matched?
Matching involves typing human leukocyte antigen (HLA) tissue. The antigens on the
surface of these special white blood cells determine the genetic makeup of a person's
immune system. There are at least 100 HLA antigens; however, it is believed that there
are a few major antigens that determine whether a donor and recipient match. The others
are considered "minor" and their effect on a successful transplant is not as well-defined.
Medical research is still investigating the role all antigens play in the process
of a bone marrow transplant. The more antigens that match, the better the engraftment
of donated marrow. Engraftment of the stem cells happens when the donated cells make
their way to the marrow and begin making new blood cells.
Most of the genes that "code" for the human immune system are on one chromosome. Since
we only have two of each chromosome, one we received from each of our parents, a full
sibling of a patient in need of a transplant has a 1 in 4 chance of having gotten
the same set of chromosomes and being a "full match" for transplantation.
The bone marrow transplant team
The group of specialists involved in the care of patients going through transplant
is often referred to as the transplant team. All individuals work together to give the best chance for a successful transplant.
The team consists of the following:
Healthcare providers. Healthcare providers who specialize in oncology, hematology, immunology, and bone
Bone marrow transplant nurse coordinator. A nurse who organizes all aspects of care provided before and after the transplant.
The nurse coordinator will provide patient education, and coordinates the diagnostic
testing and follow-up care.
Social workers. Professionals who will help your family deal with many issues that may arise, including
lodging and transportation, finances, and legal issues.
Dietitians. Professionals who will help you meet your nutritional needs before and after the
transplant. They will work closely with you and your family.
Physical therapists. Professionals who will help you become strong and independent with movement and
endurance after the transplantation.
Pastoral care. Chaplains who provide spiritual care and support.
Other team members. Several other team members will evaluate you before transplantation and will give
follow-up care as needed. These include, but are not limited to, the following:
An extensive evaluation is completed by the bone marrow transplant team. The decision
for you to undergo a bone marrow transplant will be based on many factors, including
Your age, overall health, and medical history
Extent of the disease
Availability of a donor
Your tolerance for specific medicines, procedures, or therapies
Expectations for the course of the disease
Expectations for the course of the transplant
Your opinion or preference
Preparation for the recipient
For a patient receiving the transplant, the following will occur in advance of the
Before the transplant, an extensive evaluation is completed by the bone marrow transplant
team. All other treatment choices are discussed and evaluated for risk versus benefit.
A complete medical history and physical exam are performed, including multiple tests
to evaluate the patient's blood and organ functions (for example, heart, kidney, liver,
A patient will often come into the transplant center up to 10 days before transplant
for hydration, evaluation, placement of the central venous line, and other preparations.
A catheter, also called a central venous line, is surgically placed in a vein in the
chest area. Blood products and medicines will be given through the catheter during
For an allogeneic transplant, a suitable (tissue typed and matched) donor must be
available. Finding a matching donor can be a challenging and lengthy process, especially
if a sibling match is not available. Voluntary marrow donors are registered in several
national and international registries. A bone marrow search involves searching these
registries for donors whose blood most closely resembles or matches the individual
needing the transplant.
Preparation for the donor
Donor sources available include: self, sibling, parent or relative, nonrelated person,
or umbilical cord from a related or nonrelated person. There are national and international
registries for nonrelated people and cord blood. Some family members may be typed
because of the desire to help. These relatives may or may not elect to have their
type registered for use with other recipients.
If the potential donor is notified that he or she may be a match for a patient needing
a transplant, he or she will undergo additional tests. Tests related to his or her health,
exposure to viruses, and genetic analysis will be done to determine the extent of
the match. The donor will be given instructions on how a bone marrow donation will
Once a match for a patient needing a bone marrow transplant is found, then stem cells
will be collected either by a bone marrow harvest. This is a collection of stem cells
with a needle placed into the soft center of the bone marrow. Or by a peripheral blood
stem cell collection. This is where stem cells are collected from the circulating
cells in the blood. Of the two, peripheral blood stem cell donations are now more
common. Cord blood has already been collected at the time of a birth and stored for
How are the stem cells collected?
A bone marrow transplant is done by transferring stem cells from one person to another.
Stem cells can either be collected from the circulating cells in the blood (the peripheral
system) or from the bone marrow.
Peripheral blood stem cells. Peripheral blood stem cells (PBSCs) are collected by apheresis. This is a process
in which the donor is connected to a special cell separation machine via a needle
inserted in arm veins. Blood is taken from one vein and is circulated though the machine
which removes the stem cells and returns the remaining blood and plasma back to the
donor through another needle inserted into the opposite arm. Several sessions may
be needed to collect enough stem cells to ensure a chance of successful engraftment
in the recipient.
A medicine may be given to the donor for about one week prior to apheresis that will
stimulate the bone marrow to increase production of new stem cells. These new stem
cells will be released from the marrow and into the circulating or peripheral blood
system; from there they can be collected during apheresis.
Bone marrow harvest. Bone marrow harvesting involves collecting stem cells with a needle placed into the
soft center of the bone, the marrow. Most sites used for bone marrow harvesting are
located in the hip bones and the sternum. The procedure takes place in the operating
room. The donor will be anesthetized during the harvest and will not feel the needle.
In recovery, the donor may experience some pain in the areas where the needle was
If the donor is the person himself or herself, it is called an autologous bone marrow transplant. If an autologous transplant is planned, previously collected stem cells, from either
peripheral (apheresis) or harvest, are counted, screened, and ready to infuse.
The bone marrow transplant procedure
The preparations for a bone marrow transplant vary depending on the type of transplant,
the disease needing transplant, and your tolerance for certain medicines. Consider
Most often, high doses of chemotherapy and/or radiation are included in the preparations.
This intense therapy is required to effectively treat the malignancy and make room
in the bone marrow for the new cells to grow. This therapy is often called ablative,
or myeloablative, because of the effect on the bone marrow. The bone marrow produces
most of the blood cells in our body. Ablative therapy prevents this process of cell
production and the marrow becomes empty. An empty marrow is needed to make room for
the new stem cells to grow and establish a new blood cell production system.
After the chemotherapy and/or radiation is administered, the marrow transplant is
given through the central venous catheter into the bloodstream. It is not a surgical
procedure to place the marrow into the bone, but is similar to receiving a blood transfusion.
The stem cells find their way into the bone marrow and begin reproducing and growing
new, healthy blood cells.
After the transplant, supportive care is given to prevent and treat infections, side
effects of treatments, and complications. This includes frequent blood tests, close
monitoring of vital signs, strict measurement of fluid input and output, daily weigh-ins,
and providing a protected and clean environment.
The days before transplant are counted as minus days. The day of transplant is considered
day zero. Engraftment and recovery following the transplant are counted as plus days.
For example, a patient may enter the hospital on day -8 for preparative regimen. The
day of transplant is numbered zero. Days +1, +2, etc., will follow. There are specific
events, complications, and risks associated with each day before, during, and after
transplant. The days are numbered to help the patient and family understand where
they are in terms of risks and discharge planning.
During infusion of bone marrow, the patient may experience the following:
After infusion, the patient may:
Spend several weeks in the hospital
Be very susceptible to infection
Experience excessive bleeding
Need blood transfusions
Be confined to a clean environment
Take multiple antibiotics and other medicines
Be given medicine to prevent graft-versus-host disease—if the transplant was allogeneic.
The transplanted new cells (the graft) tend to attack the patient's tissues (the host),
even if the donor is a relative.
Undergo continual laboratory testing
Experience nausea, vomiting, diarrhea, mouth sores, and extreme weakness
Experience temporary mental confusion and emotional or psychological distress
After leaving the hospital, the recovery process continues for several months or longer,
during which time the patient cannot return to work or many previously enjoyed activities.
The patient must also make frequent follow-up visits to the hospital or healthcare
When does engraftment happen?
Engraftment of the stem cells happens when the donated cells make their way to the
marrow and begin making new blood cells. Depending on the type of transplant and the
disease being treated, engraftment usually happens around day +15 or +30. Blood counts
will be checked often during the days following transplant to evaluate initiation
and progress of engraftment. Platelets are generally the last blood cell to recover.
Engraftment can be delayed because of infection, medicines, low donated stem cell
count, or graft failure. Although the new bone marrow may begin making cells in the
first 30 days following transplant, it may take months, even years, for the entire
immune system to fully recover.
What complications and side effects may happen following BMT?
Complications may vary, depending on the following:
Type of marrow transplant
Type of disease requiring transplant
Age and overall health of the recipient
Variance of tissue matching between donor and recipient
Presence of severe complications
The following are complications that may happen with a bone marrow transplant. However,
each individual may experience symptoms differently. These complications may also
happen alone, or in combination:
Infections. Infections are likely in the patient with severe bone marrow suppression. Bacterial
infections are the most common. Viral and fungal infections can be life-threatening.
Any infection can cause an extended hospital stay, prevent or delay engraftment, and/or
cause permanent organ damage. Antibiotics, antifungal medicines, and antiviral medicines
are often given to try to prevent serious infection in the immunosuppressed patient.
Low platelets and low red blood cells. Thrombocytopenia (low platelets) and anemia (low red blood cells), as a result of
a nonfunctioning bone marrow, can be dangerous and even life-threatening. Low platelets
can cause dangerous bleeding in the lungs, gastrointestinal (GI) tract, and brain.
Pain. Pain related to mouth sores and gastrointestinal (GI) irritation is common. High doses
of chemotherapy and radiation can cause severe mucositis (inflammation of the mouth
and GI tract).
Fluid overload. Fluid overload is a complication that can lead to pneumonia, liver damage, and high
blood pressure. The main reason for fluid overload is because the kidneys cannot keep
up with the large amount of fluid being given in the form of intravenous (IV) medicines,
nutrition, and blood products. The kidneys may also be damaged from disease, infection,
chemotherapy, radiation, or antibiotics.
Respiratory distress. Respiratory status is an important function that may be compromised during transplant.
Infection, inflammation of the airway, fluid overload, graft-versus-host disease,
and bleeding are all potential life-threatening complications that may happen in the
lungs and pulmonary system.
Organ damage. The liver and heart are important organs that may be damaged during the transplantation
process. Temporary or permanent damage to the liver and heart may be caused by infection,
graft-versus-host disease, high doses of chemotherapy and radiation, or fluid overload.
Graft failure. Failure of the graft (transplant) taking hold in the marrow is a potential complication.
Graft failure may happen as a result of infection, recurrent disease, or if the stem
cell count of the donated marrow was insufficient to cause engraftment.
Graft-versus-host disease. Graft-versus-host disease (GVHD) can be a serious and life-threatening complication
of a bone marrow transplant. GVHD occurs when the donor's immune system reacts against
the recipient's tissue. As opposed to an organ transplant where the patient's immune
system will attempt to reject only the transplanted organ, in GVHD the new or transplanted
immune system can attack the entire patient and all of his or her organs. This is
because the new cells do not recognize the tissues and organs of the recipient's body as
self. Over time and with the help of medicines to suppress the new immune system,
it will begin to accept its new body and stop attacking it. The most common sites
for GVHD are GI tract, liver, skin, and lungs.
Long-term outlook for a bone marrow transplantation
Prognosis greatly depends on the following:
Type of transplant
Type and extent of the disease being treated
Disease response to treatment
Your age and overall health
Your tolerance of specific medicines, procedures, or therapies
Severity of complications
As with any procedure, in bone marrow transplant the prognosis and long-term survival
can vary greatly from person to person. The number of transplants being done for an
increasing number of diseases, as well as ongoing medical developments, have greatly
improved the outcome for bone marrow transplant in children and adults. Continuous
follow-up care is essential for the patient following a bone marrow transplant. New
methods to improve treatment and to decrease complications and side effects of a bone
marrow transplant are continually being discovered.