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Gonzo Configuration

This instrument is no longer in service. This page will remain active as a reference only.

LSR-II Configuration:

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

BLUE A

735

780/60

PE-Cy7, PE-Alexa750

650

BLUE B

685

710/50

PerCP-Cy5.5, PE-Cy5.5, PerCP, 7AAD, PE-Alexa700, PE-Alexa680

550

BLUE C

665 660/20 PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ 550

BLUE D

600

610/20

630/30

PI, PE-Texas Red, PE-Alexa610, PE-Alexa594

550

BLUE E

550

576/26

PE

400

BLUE F

505

525/50

FITC, Alexa488, YFP, GFP, CFSE

450
BLUE G   488/10 SSC  

RED 633 LASER (20mW)

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

RED A

735

780/60

APC-Cy7, APC-Alexa750, APC-H7

550

RED B

685 710/50 APC-Cy5.5, AlexaFluor 700, AlexaFluor 680 550

RED C

 

660/20

APC, Alexa 647

450

VIOLET 405 LASER (40mw)

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

VIOLET A

595

610/20

Qdot 605

450

VIOLET B

505

525/50

AlexaFluor 430, CFP, Fura-Hich_Ca++, Pacific Orange

450

VIOLET C

 

450/50

AlexaFluor 405, DAPI, BV 421, Pacific Blue, Sytox Blue

400