Originally sponsored by the James P. Wilmont Cancer Center, the acquisition and upgrading of the Amnis ImageStream was supported by NIH Grant 1 S10 RR023458-01A1 (C Jordan PI).
Supported in part by the University of Rochester Developmental Center for AIDS Research grant P30 AI078498 (NIH/NIAID) and the University of Rochester School of Medicine and Dentistry.
Amnis - ImageStream GenX
Amnis ImageStream, a hybrid fluorescent microscope/flow cytometer. With this technology, one can collect morphological data on cells stained with up to four different fluorochromes at 5,000 events per minute. Coupled with the analytical packages, IDEAS, statistical rigor can be brought to answer questions such as:
- sub-cellular protein localization
- surface maker co-localization
- marker asymmetry
- molecular trafficking
- Phagocytosis ll
Lasers include 405nm, 488nm, 594nm, and 658 nm
To design a panel using Fluorofinder please click here.
Channels 1-6 are primarily used for the 488nm laser. Channels 7-8 are primarily for the 405 laser, channels 9-10 for the 594nm laser, and 11-12 for the 658nm laser. There are multiple channels that have multiple or cross laser excitation that compensation will take care of. On the Fluorochrome List at the bottom of the page, the common dyes visible in each channel are listed, along with the secondary laser excitation. For more information, contact Matt Cochran or Wojtek.
|Channel 1||Channel 2||Channel 3||Channel 4||Channel 5||Channel 6|
|Channel 7||Channel 8||Channel 9||Channel 10||Channel 11||Channel 12|
Use the following link to access the full list of fluors usable on this instrument. If a fluor you would like to use is not listed, contact Matt Cochran or Wojtek in the Flow Core.
Location/Time - Rm 3-4103 @10am (in the flow core hallway)
Schedule: Jan 23, Feb 6 and 20.
next lecture coming soon!
Spectral Viewers and Panel Design
All guidelines are available in the "User Docs" folder located on every instrument computer as well as in the FCC_Library server folder.
Comments about the Flow Core?