Kermit Configuration

Suggested voltages current as of Dec 31, 2015.

Detailed information about this instrument can be found on the baseline report (available upon request).

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

To design a panel using Fluorofinder please click here.

 

 

LSR-II Configuration: 

488 Laser  || 633 Laser  || 405 Laser

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

BLUE A

735

780/60

PE-Cy7, PE-Alexa750

600

BLUE B

685

710/50

PerCP-Cy5.5, PE-Cy5.5, PerCP, PE-Alexa700, PE-Alexa680

550

BLUE C

665 660/20 PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ 550

BLUE D

600

610/20

630/30

PI, PE-Texas Red, PE-Alexa610, PE-Alexa594

500

BLUE E

550

576/26

PE

550

BLUE F

505

525/50

FITC, Alexa488, YFP, GFP, CFSE

500
BLUE G   488/10 SSC  

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

RED A

735

780/60

APC-Cy7, APC-Alexa750, APC-H7

550

RED B

685 710/50 APC-Cy5.5, AlexaFluor 700, AlexaFluor 680 500

RED C

660/20

APC, Alexa 647

450

VIOLET 405 LASER (40mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

VIOLET A

595

610/20

Qdot 605

550

VIOLET B

505

525/50

AlexaFluor 430, Fura-Hich_Ca++, Pacific Orange

500

VIOLET C

450/50

AlexaFluor 405, DAPI, BV 421, Pacific Blue, Sytox Blue

400