Canto Training

Introduction to Flow Lecture

Before you can sign up for initial training, you will be required to attend the Introduction to Flow lecture. This is a 1 hour basic lecture to introduce you to the concepts of flow cytometry. Training will occur in 3-4103 in the Flow Core hallway at 4pm every other Monday. There is no registration for the lecture and all are eligible to attend. Please be sure to have reviewed the material in the BD Introduction to Flow pdf.


The following documents will either be needed during training or are a useful resource before you come for training. All documents can be found in the FCC_Library here.

  1. URMC Introduction to Flow Lecture
  2. BD FACSCanto II User Information
  3. BD Intro to Flow tutorial
  4. Flow Core's FACSDiva software guide

Training Availability

Training for the Accuri is done on a flexible basis based on the availibility of the instrument and an instructor.

Before Canto Training

The user must complete the LSR training before Canto training can be done.

Canto Training Visit

This is for trained LSR users that would also like to learn the FACSCanto operation. The FACSDiva software on the Canto is very similar to that found on the LSR but operation of the instrument is different.

  1. Fill out a training request form to schedule an appointment.
  2. This visit is 30 minutes where the user will run beads under the supervision of an instructor.
  3. Upon completion of this visit, calendar access for the Canto will be activated.

Calendar Access

After Canto training, the trainee will have calendar access and schedule/run experiments during normal business hours (8am-5pm).

After-Hours Access

The Flow Core is open during normal business hours (8am to 5pm) from Monday through Friday.

To receive after-hours access, the investigator must fill out the afterhours access form. A separate training session (free, approximately ½ hour) will be scheduled to review the specifics of after-hours access and instrument troubleshooting. After this session, the investigator must pass a written afterhours access exam. There is no practical for the Canto. If they fail, they must wait two weeks to reschedule the test. An investigator can schedule this test any time after they have normal access to the instrument.

Helpful Training Links

Upcoming Events

Intro Lecture:

Location/Time - Rm 3-4103 @10am (in the flow core hallway)

Schedule: Feb 1st, 15th and 29th

Lecture series

next lecture coming soon!


Featured Article

Niswander et al., Cytometry


Spectral Viewers

Spectra Viewer (via Invitrogen)

Spectra Viewer (via BD Biosciences)

Spectra Viewer (via EBioscience)


Instrument Guidelines

All guidelines are available in the "User Docs" folder located on every instrument computer as well as in the FCC_Library server folder.


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