Fee Structure A screening project consists generally consists of three phases. Assay development. In order to perform a screen an assay that is suitable for high throughput screening must be developed. Users must develop their own assays but must be realistic about what assays are suitable for HTS. The facility will provide advice on assay development and HTS facility equipment can be used to develop the assay. In the end the assay must be simple and robust. The amount of time needed for assay optimization, miniaturization, and validation varies depending on the assay variables and technology associated with it. Pricing for assay development is $55/hr of labor. Small scale Screen of Spectrum library. We recommend a 2000 molecule screen of the Spectrum library.Pricing for this screen would be approximately $1500 Large scale Screen of Chembridge library. 5000-20,000 compounds Pricing for 20,000 compounds would be approximately $5250 Included in the price of the screen are cherry picking of the top 20 compound hits and confirmation by assay repeat. An extra charge will apply for dose responses and hit validation beyond the first 20 compounds. Equipment Rental Equipment can also be used on a time basis for limited projects depending on availability with close supervision by the facility director. Equipment Pricing/Hr Janus Robot $60.00 Envision Plate Reader $20.00 FlexDrop Liquid dispenser $20.00 Consumables and Reagents Users will be responsible for the costs of consumables and reagents required to conduct the screen. Major consumable costs can include assay plates, pipette tips and assay reagents.