Immune Cells and Disease
Our lab is focused on understanding the complex interactions among immune cells that take place in target tissues. We have a particular interest on defining the distinctive and strategic organization of immune cells in the peripheral tissues and how they contribute to the local induction of pathological or protective immune responses.
Role of Bronchus Associated Lymphoid Tissue in Pulmonary Immunity
In a collaborative project with Dr. Shabaana Abdul Khader at Washington University in Saint Louis and with support from the NIH, we are defining the contribution of inducible bronchus associated lymphoid tissue (iBALT) in the control of experimental pulmonary tuberculosis. Additionally, in a recent collaboration with Dr. Taylor Edens and Dr. Jay Kolls, we defined how IL17 and type 2 cytokines support IBALT formation during Pneumocystis murina infection (Cell Rep 18(13): 3078-3090:2017).
Impact of Radiation on Immunity
Preliminary data in our lab have shown a long-lasting impact of neonatal irradiation on the CD4 T cell response against influenza viruses. Although defective CD4 T cells response did not compromise immunity to influenza viruses, it is possible that CD4 T cell-mediated protection against other extracellular pulmonary pathogens can be compromised by exposure to radiation in neonatal life (Radiat Res 184(4): 352-366: 2015). Thus, we are currently focused to investigate the effect of neonatal irradiation in the control of clinically relevant pulmonary pathogens.
Tertiary Lymphoid Organs in Cancer
In a recent collaboration with researchers from University of Washington in Saint Louis (Shabaana Abdul Khader), University of Southern California in los Angeles (Martin W. Kast) and colleagues from the National Institute of Medical Sciences and Nutrition in Mexico City (Norma Ofelia Uribe Uribe, Ricardo Espinosa-Gonzalez), we demonstrated the existence of a unique microenvironment that is associated with the spontaneous regression of prostate cancer. Picture from TLO in evanescent prostate carcinoma (Front Immunol 8: 563: 2017).
Evaluation of Experimental Therapies in a Preclinical Model of Lupus
In collaboration with Dr. Allan Smrcka, we previously demonstrated that inhibition of G protein signaling prevents lupus nephritis by targeting the migration of immune cells and impairing auto-reactive germinal centers (Arthritis Rheumatol 68(9): 2244-2256: 2016). In a similar fashion, experimental treatment with inhibitors of nuclear disrupts splenic germinal centers and the niche for auto-reactive and inflammatory cells in the inflamed kidney of lupus prone mice. Picture from plasma cells after treatment with selective inhibitors of nuclear export.
Use of Laser-capture Microdissection to Decipher RNA Signatures in Target Tissues
In a project supported by the AMP and NIH, we are dissecting the molecular signatures of infiltrating lymphocytes in the inflamed synovium of patients with rheumatoid arthritis by using immunofluorescence and laser capture microdissection. The purpose is to reveal molecules that can be used as markers of disease progression/severity and identify novel targets to increase the therapeutic armamentarium for rheumatoid arthritis.