M Anwar Iqbal, Ph.D.
India-Osmania University, Gandhi Medical College
Department of Pathology and Laboratory Medicine
The Iqbal Laboratory provides microarray comparative genomic hybridization (microarray CGH) testing for postnatal, prenatal and cancer patients. As part of URMC’s comprehensive pathology and laboratory medicine department, the lab helps clinical geneticists, reproductive geneticists, pediatric geneticists, neonatologists, clinical oncologists and haematopathologists provide the best patient care management.
The microarray CGH lab receives peripheral blood, amniotic fluid, chorionic villus, products of conception, bone marrow, formalin-fixed, paraffin embedded (FFPE) and solid tumor samples for DNA microarray testing. Testing determines the loss or gain of DNA segments in the patient compared to normal controls. These changes, known as copy number variations (CNVs), have been associated with susceptibility to certain disorders as well as reactions to drugs that treat these disorders.
Our associated research involves the following:
- Evaluation of various CGH platforms and methodologies.
- Studies of the efficacy of microarray-based testing for detection of genetic aberrations in various types of malignancies.
Microarray technology allows the comparison of a specific segment of DNA to thousands of probes. Procedural issues include questions such as, “What are the most useful probes for clinical testing, that yield the clearest results and point to possible therapies?” Data resulting from multiple tests of specific tumor types may also uncover new areas of genomic interest for basic researchers. Our laboratory is collaborating with Dr. Marilyn Li of Tulane University and with the CCMC in these areas.
The microarray lab is the only lab in the Upstate NY area providing in-house and reference testing for patients with autism, dysmorphic features and mental retardation, and for fetuses with multiple ultrasound abnormalities. This has allowed the accumulation of significant data for further studies.
Evans AG, Ahmad A, Burack WR, Iqbal MA. "Combined comparative genomic hybridization and single-nucleotide polymorphism array detects cryptic chromosomal lesions in both myelodysplastic syndromes and cytopenias of undetermined significance." Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.. 2016 Oct 0; 29(10):1183-99. Epub 2016 Jul 08.
Ahmad A; Battacharya S; Sridhar A; Iqbal MA and Mariani TJ. "Recurrent Copy Number Variation Associated with Bronchopulmonary Dysplasia". Pediatr Res. 2016; 79((6)): 940-5.
Petersen AK, Ahmad A, Shafiq M, Brown-Kipphut B, Fong CT, Anwar Iqbal M. "Deletion 1q43 encompassing only CHRM3 in a patient with autistic disorder." European journal of medical genetics.. 2013 Feb 0; 56(2):118-22. Epub 2012 Dec 16.
Coppage M, Iqbal A, Ahmad A, Becker MW. "Leukemia specific loss of heterozygosity of MHC in a CLL patient: disease state impacts timing of confirmatory typing." Human immunology.. 2013 Jan 0; 74(1):41-4. Epub 2012 Oct 17.
Velinov M, Ahmad A, Brown-Kipphut B, Shafiq M, Blau J, Cooma R, Roth P, Iqbal MA. "A 0.7?Mb de novo duplication at 7q21.3 including the genes DLX5 and DLX6 in a patient with split-hand/split-foot malformation." American journal of medical genetics. Part A.. 2012 Dec 0; 158A(12):3201-6. Epub 2012 Nov 20.
Vargas H, Beldia G, Korosh W, Sudhalter V, Iqbal A, Sanchez-Lacay JA, Velinov M. "A 4.5 Mb terminal deletion of chromosome 12p helps further define a psychosis-associated locus." European journal of medical genetics.. 2012 Oct 0; 55(10):573-6. Epub 2012 Jun 02.
Cherry AM, Slovak ML, Campbell LJ, Chun K, Eclache V, Haase D, Haferlach C, Hildebrandt B, Iqbal AM, Jhanwar SC, Ohyashiki K, Sole F, Vandenberghe P, VanDyke DL, Zhang Y, Dewald GW. "Will a peripheral blood (PB) sample yield the same diagnostic and prognostic cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia?" Leukemia research.. 2012 Jul 0; 36(7):832-40. Epub 2012 Apr 25.
Johnson MD, O'Connell M, Iqbal MA, Williams JP. "Radiation effects on human leptomeningeal cell response to cerebrospinal fluid and PDGF-BB." International journal of radiation biology.. 2012 Jul 0; 88(7):547-55. Epub 2012 May 25.
Ahmad A, Iqbal MA. "Significance of genome-wide analysis of copy number alterations and UPD in myelodysplastic syndromes using combined CGH - SNP arrays." Current medicinal chemistry.. 2012 19(22):3739-47.
Society of Human Genetics Annual Meeting, Washington, DC, Nov. 3-6, 2010 (Platform Presentation)