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J Edward Puzas, Ph.D.

J Edward Puzas, Ph.D.

Research Lab

The Center for Musculoskeletal Research houses nearly 70 MD and PhD researchers and graduate and post-doctoral students. This team has been the top orthopaedics lab in the United States since 2006.

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About Me

Faculty Appointments

Professor (Part-Time) - Department of Orthopaedics, Center for Musculoskeletal Research (SMD)

Credentials

Education

PhD | University of Rochester School of Medicine and Dentistry. Radiation Biology and Biophysics. 1976

MS | University of Rochester School of Medicine and Dentistry. Radiation Biology and Biophysics. 1973

BS | University of Massachusetts. Zoology. 1971

Research

Patents

Correction of Dual energy X-Ray Absorptiometry Measurements Base on Body Lead Levels

Issue date: June 10, 2008

Patent #: 7,386,337

Country: United States

Inventors: J. Edward Puzas

Compositions and Methods Involved in Bone Growth

Issue date: July 10, 2007

Patent #: 7,241,732

Country: United States

Inventors: J. Edward Puzas

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: May 30, 2018

Patent #: 2349325

Country: Europe

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: August 20, 2013

Patent #: 8,513,193

Country: United States

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: November 24, 2015

Patent #: 9,192,653

Country: United States

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Compositions and Methods for Controlled Localized Delivery of Bone Forming Therapeutic Agents

Issue date: April 24, 2018

Patent #: 9,949,950

Country: United States

Inventors: Danielle SW Benoit, Maureen Newman, J. Edward Puzas, Tzong-Jen Sheu

Compositions and Methods for Controlled Localized Delivery of Bone Forming Therapeutic Agents

Issue date: February 05, 2019

Patent #: 10,195,284

Country: United States

Inventors: Danielle SW Benoit, Benjamin J Frisch, Maureen Newman, J. Edward Puzas, Tzong-Jen Sheu

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: May 30, 2018

Patent #: FR2349325

Country: France

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: May 30, 2018

Patent #: 602009052599.9

Country: Germany

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Issue date: May 30, 2018

Patent #: GB2349325

Country: United Kingdom

Inventors: Hani A Awad, Susan Bukata, J. Edward Puzas, Randy N Rosier, Erik R Sampson, Michael J Zuscik

Publications

Journal Articles

Quantitative mouse model of implant-associated osteomyelitis and the kinetics of microbial growth, osteolysis, and humoral immunity.

Li D, Gromov K, Søballe K, Puzas JE, O'Keefe RJ, Awad H, Drissi H, Schwarz EM

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2008 January 26 (1):96-105. Epub 1900 01 01.

Natural calcium isotopic composition of urine as a marker of bone mineral balance.

Skulan J, Bullen T, Anbar AD, Puzas JE, Shackelford L, LeBlanc A, Smith SM

Clinical chemistry.. 2007 June 53 (6):1155-8. Epub 04/26/2007.

Nano to microscale porous silicon as a cell interface for bone tissue engineering.

Sun W; Puzas JE; Sheu TJ; Liu X; Fauchet PM.

Advanced Materials. 2007; .

Molecular correlates of site-specific metastasis.

Dai CY, Haqq CM, Puzas JE

Seminars in radiation oncology.. 2006 April 16 (2):102-10. Epub 1900 01 01.

Intraarticular induction of interleukin-1beta expression in the adult mouse, with resultant temporomandibular joint pathologic changes, dysfunction, and pain.

Lai YC, Shaftel SS, Miller JN, Tallents RH, Chang Y, Pinkert CA, Olschowka JA, Dickerson IM, Puzas JE, O'Banion MK, Kyrkanides S

Arthritis and rheumatism.. 2006 April 54 (4):1184-97. Epub 1900 01 01.

Accelerated fracture healing.

Puzas JE, Houck J, Bukata SV

The Journal of the American Academy of Orthopaedic Surgeons.. 2006 14 (10 Spec No.):S145-51. Epub 1900 01 01.

A genome-wide expression profile and system-level integration of nuclear factor kappa B regulated genes reveals fundamental metabolic adaptations during cell growth and survival.

Andela VB, Schwarz EM, O'Keefe RJ, Puzas EJ, Rosenblatt JD, Rosier RN

FEBS letters.. 2005 December 19579 (30):6814-20. Epub 11/28/2005.

Lead exposure inhibits fracture healing and is associated with increased chondrogenesis, delay in cartilage mineralization, and a decrease in osteoprogenitor frequency.

Carmouche JJ, Puzas JE, Zhang X, Tiyapatanaputi P, Cory-Slechta DA, Gelein R, Zuscik M, Rosier RN, Boyce BF, O'Keefe RJ, Schwarz EM

Environmental health perspectives.. 2005 June 113 (6):749-55. Epub 1900 01 01.

Nuclear factor-kappaB-dependent mechanisms in breast cancer cells regulate tumor burden and osteolysis in bone.

Gordon AH, O'Keefe RJ, Schwarz EM, Rosier RN, Puzas JE

Cancer research.. 2005 April 1565 (8):3209-17. Epub 1900 01 01.

The role of Axin2 in calvarial morphogenesis and craniosynostosis.

Yu HM, Jerchow B, Sheu TJ, Liu B, Costantini F, Puzas JE, Birchmeier W, Hsu W

Development.. 2005 April 132 (8):1995-2005. Epub 1900 01 01.

The association between environmental lead exposure and bone density in children.

Campbell JR, Rosier RN, Novotny L, Puzas JE

Environmental health perspectives.. 2004 August 112 (11):1200-3. Epub 1900 01 01.

Transforming growth factor-beta1 induced alteration of skeletal morphogenesis in vivo.

Ferguson CM, Schwarz EM, Puzas JE, Zuscik MJ, Drissi H, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2004 July 22 (4):687-96. Epub 1900 01 01.

5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes.

Zuscik MJ, Baden JF, Wu Q, Sheu TJ, Schwarz EM, Drissi H, O'Keefe RJ, Puzas JE, Rosier RN

Journal of cellular biochemistry.. 2004 May 1592 (2):316-31. Epub 1900 01 01.

Primary murine limb bud mesenchymal cells in long-term culture complete chondrocyte differentiation: TGF-beta delays hypertrophy and PGE2 inhibits terminal differentiation.

Zhang X, Ziran N, Goater JJ, Schwarz EM, Puzas JE, Rosier RN, Zuscik M, Drissi H, O'Keefe RJ

Bone.. 2004 May 34 (5):809-17. Epub 1900 01 01.

CREB Cooperates with BMP-stimulated Smad signaling to enhance transcription of the Smad6 promoter.

Ionescu AM, Drissi H, Schwarz EM, Kato M, Puzas JE, McCance DJ, Rosier RN, Zuscik MJ, O'Keefe RJ

Journal of cellular physiology.. 2004 March 198 (3):428-40. Epub 1900 01 01.

PGE2 and IL-6 production by fibroblasts in response to titanium wear debris particles is mediated through a Cox-2 dependent pathway.

Bukata SV, Gelinas J, Wei X, Rosier RN, Puzas JE, Zhang X, Schwarz EM, Song XY, Griswold DE, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2004 January 22 (1):6-12. Epub 1900 01 01.

Runx2/Cbfa1 stimulation by retinoic acid is potentiated by BMP2 signaling through interaction with Smad1 on the collagen X promoter in chondrocytes.

Drissi MH, Li X, Sheu TJ, Zuscik MJ, Schwarz EM, Puzas JE, Rosier RN, O'Keefe RJ

Journal of cellular biochemistry.. 2003 December 1590 (6):1287-98. Epub 1900 01 01.

Pharmacologic modulators of fracture healing: the role of cyclooxygenase inhibition.

Puzas JE, O'Keefe RJ, Schwarz EM, Zhang X

Journal of musculoskeletal & neuronal interactions.. 2003 December 3 (4):308-12; discussion 320-1. Epub 1900 01 01.

Biology and pathology of the temporomandibular joint: is there an animal model to study this affliction?

Puzas JE

Journal of musculoskeletal & neuronal interactions.. 2003 December 3 (4):395-400; discussion 406-7. Epub 1900 01 01.

The mevalonate synthesis pathway as a therapeutic target in cancer.

Andela VB, Pirri M, Schwarz EM, Puzas EJ, O'Keefe RJ, Rosenblatt JD, Rosier RN

Clinical orthopaedics and related research.. 2003 October (415 Suppl):S59-66. Epub 1900 01 01.

NFkappaB: a pivotal transcription factor in prostate cancer metastasis to bone.

Andela VB, Gordon AH, Zotalis G, Rosier RN, Goater JJ, Lewis GD, Schwarz EM, Puzas JE, O'Keefe RJ

Clinical orthopaedics and related research.. 2003 October (415 Suppl):S75-85. Epub 1900 01 01.

Smad6 is induced by BMP-2 and modulates chondrocyte differentiation.

Li X, Ionescu AM, Schwarz EM, Zhang X, Drissi H, Puzas JE, Rosier RN, Zuscik MJ, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2003 September 21 (5):908-13. Epub 1900 01 01.

ALK2 functions as a BMP type I receptor and induces Indian hedgehog in chondrocytes during skeletal development.

Zhang D, Schwarz EM, Rosier RN, Zuscik MJ, Puzas JE, O'Keefe RJ

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 2003 September 18 (9):1593-604. Epub 1900 01 01.

ATF-2 cooperates with Smad3 to mediate TGF-beta effects on chondrocyte maturation.

Ionescu AM, Schwarz EM, Zuscik MJ, Drissi H, Puzas JE, Rosier RN, O'Keefe RJ

Experimental cell research.. 2003 August 1288 (1):198-207. Epub 1900 01 01.

Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.

Boyan BD, Schwartz Z, Lohmann CH, Sylvia VL, Cochran DL, Dean DD, Puzas JE

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2003 July 21 (4):638-47. Epub 1900 01 01.

Retinoic acid stimulates chondrocyte differentiation and enhances bone morphogenetic protein effects through induction of Smad1 and Smad5.

Li X, Schwarz EM, Zuscik MJ, Rosier RN, Ionescu AM, Puzas JE, Drissi H, Sheu TJ, O'Keefe RJ

Endocrinology.. 2003 June 144 (6):2514-23. Epub 1900 01 01.

Clinical relevance of increased retinoid and cAMP transcriptional programs in tumor cells rendered non-malignant by dominant negative inhibition of NFkappaB.

Andela VB, Gingold BI, Souza MD, O'Keefe RJ, Puzas EJ, Schwarz EM, Rosier RN

Cancer letters.. 2003 May 8194 (1):37-43. Epub 1900 01 01.

A phage display technique identifies a novel regulator of cell differentiation.

Sheu TJ, Schwarz EM, Martinez DA, O'Keefe RJ, Rosier RN, Zuscik MJ, Puzas JE

The Journal of biological chemistry.. 2003 January 3278 (1):438-43. Epub 10/25/2002.

Malignant reversion of a human osteosarcoma cell line, Saos-2, by inhibition of NFkappaB.

Andela VB, Sheu TJ, Puzas EJ, Schwarz EM, O'Keefe RJ, Rosier RN

Biochemical and biophysical research communications.. 2002 September 20297 (2):237-41. Epub 1900 01 01.

Parathyroid hormone-related peptide regulation of chick tibial growth plate chondrocyte maturation requires protein kinase A.

Zuscik MJ, O'Keefe RJ, Gunter TE, Puzas JE, Schwarz EM, Rosier RN

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2002 September 20 (5):1079-90. Epub 1900 01 01.

The role of p105 protein in NFkappaB activation in ANA-1 murine macrophages following stimulation with titanium particles.

Soloviev A, Schwarz EM, Kuprash DV, Nedospasov SA, Puzas JE, Rosier RN, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2002 July 20 (4):714-22. Epub 1900 01 01.

Lead alters parathyroid hormone-related peptide and transforming growth factor-beta1 effects and AP-1 and NF-kappaB signaling in chondrocytes.

Zuscik MJ, Pateder DB, Puzas JE, Schwarz EM, Rosier RN, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2002 July 20 (4):811-8. Epub 1900 01 01.

Growth plate chondrocyte maturation is regulated by basal intracellular calcium.

Zuscik MJ, D'Souza M, Ionescu AM, Gunter KK, Gunter TE, O'Keefe RJ, Schwarz EM, Puzas JE, Rosier RN

Experimental cell research.. 2002 June 10276 (2):310-9. Epub 1900 01 01.

Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair.

Zhang X, Schwarz EM, Young DA, Puzas JE, Rosier RN, O'Keefe RJ

The Journal of clinical investigation.. 2002 June 109 (11):1405-15. Epub 1900 01 01.

Use of a phage display technique to identify potential osteoblast binding sites within osteoclast lacunae.

Sheu TJ, Schwarz EM, O'Keefe RJ, Rosier RN, Puzas JE

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 2002 May 17 (5):915-22. Epub 1900 01 01.

Viral interleukin-10 gene inhibition of inflammation, osteoclastogenesis, and bone resorption in response to titanium particles.

Carmody EE, Schwarz EM, Puzas JE, Rosier RN, O'Keefe RJ

Arthritis and rheumatism.. 2002 May 46 (5):1298-308. Epub 1900 01 01.

Synergism of aminobisphosphonates and farnesyl transferase inhibitors on tumor metastasis.

Andela VB, Rosenblatt JD, Schwarz EM, Puzas EJ, O'Keefe RJ, Rosier RN

Clinical orthopaedics and related research.. 2002 April (397):228-39. Epub 1900 01 01.

Immunohistochemical study of receptor activator of nuclear factor kappa-B ligand (RANK-L) in human osteolytic bone tumors.

Good CR, O'Keefe RJ, Puzas JE, Schwarz EM, Rosier RN

Journal of surgical oncology.. 2002 March 79 (3):174-9. Epub 1900 01 01.

Efficacy of ex vivo OPG gene therapy in preventing wear debris induced osteolysis.

Goater JJ, O'Keefe RJ, Rosier RN, Puzas JE, Schwarz EM

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2002 March 20 (2):169-73. Epub 1900 01 01.

In vivo RANK signaling blockade using the receptor activator of NF-kappaB:Fc effectively prevents and ameliorates wear debris-induced osteolysis via osteoclast depletion without inhibiting osteogenesis.

Childs LM, Paschalis EP, Xing L, Dougall WC, Anderson D, Boskey AL, Puzas JE, Rosier RN, O'Keefe RJ, Boyce BF, Schwarz EM

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 2002 February 17 (2):192-9. Epub 1900 01 01.

A role for the BMP antagonist chordin in endochondral ossification.

Zhang D, Ferguson CM, O'Keefe RJ, Puzas JE, Rosier RN, Reynolds PR

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 2002 February 17 (2):293-300. Epub 1900 01 01.

Role of pentoxifylline in preventing radiation damage to epiphyseal growth plate chondrocytes.

Pateder DB, Sheu TJ, O'Keefe RJ, Puzas JE, Schwarz EM, Constine LS, Okunieff P, Rosier RN

Radiation research.. 2002 January 157 (1):62-8. Epub 1900 01 01.

The orthopaedic genome: what does the future hold and are we ready?

Puzas JE, O'Keefe RJ, Lieberman JR

The Journal of bone and joint surgery. American volume.. 2002 January 84-A (1):133-41. Epub 1900 01 01.

COX-1 and COX-2 expression in osteoid osteomas.

Mungo DV, Zhang X, O'Keefe RJ, Rosier RN, Puzas JE, Schwarz EM

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2002 January 20 (1):159-62. Epub 1900 01 01.

PTHrP expression in chick sternal chondrocytes is regulated by TGF-beta through Smad-mediated signaling.

Pateder DB, Ferguson CM, Ionescu AM, Schwarz EM, Rosier RN, Puzas JE, O'Keefe RJ

Journal of cellular physiology.. 2001 September 188 (3):343-51. Epub 1900 01 01.

Oral pentoxifylline inhibits release of tumor necrosis factor-alpha from human peripheral blood monocytes : a potential treatment for aseptic loosening of total joint components.

Pollice PF, Rosier RN, Looney RJ, Puzas JE, Schwarz EM, O'Keefe RJ

The Journal of bone and joint surgery. American volume.. 2001 July 83-A (7):1057-61. Epub 1900 01 01.

The role of autocrine growth factors in radiation damage to the epiphyseal growth plate.

Pateder DB, Eliseev RA, O'Keefe RJ, Schwarz EM, Okunieff P, Constine LS, Puzas JE, Rosier RN

Radiation research.. 2001 June 155 (6):847-57. Epub 1900 01 01.

PTHrP modulates chondrocyte differentiation through AP-1 and CREB signaling.

Ionescu AM, Schwarz EM, Vinson C, Puzas JE, Rosier R, Reynolds PR, O'Keefe RJ

The Journal of biological chemistry.. 2001 April 13276 (15):11639-47. Epub 01/02/2001.

Evidence for a direct role of cyclo-oxygenase 2 in implant wear debris-induced osteolysis.

Zhang X, Morham SG, Langenbach R, Young DA, Xing L, Boyce BF, Puzas EJ, Rosier RN, O'Keefe RJ, Schwarz EM

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 2001 April 16 (4):660-70. Epub 1900 01 01.

BMP signaling stimulates chondrocyte maturation and the expression of Indian hedgehog.

Grimsrud CD, Romano PR, D'Souza M, Puzas JE, Schwarz EM, Reynolds PR, Roiser RN, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2001 January 19 (1):18-25. Epub 1900 01 01.

Development of an animal model for prostate cancer cell metastasis to adult human bone.

Tsingotjidou AS, Zotalis G, Jackson KR, Sawyers C, Puzas JE, Hicks DG, Reiter R, Lieberman JR

Anticancer research.. 2001 21 (2A):971-8. Epub 1900 01 01.

Degradative pathways in tissues of the temporomandibular joint. Use of in vitro and in vivo models to characterize matrix metalloproteinase and cytokine activity.

Puzas JE, Landeau JM, Tallents R, Albright J, Schwarz EM, Landesberg R

Cells, tissues, organs. 2001 169 (3):248-56. Epub 1900 01 01.

Tumor metastasis and the reciprocal regulation of prometastatic and antimetastatic factors by nuclear factor kappaB.

Andela VB, Schwarz EM, Puzas JE, O'Keefe RJ, Rosier RN

Cancer research.. 2000 December 160 (23):6557-62. Epub 1900 01 01.

Smad2 and 3 mediate transforming growth factor-beta1-induced inhibition of chondrocyte maturation.

Ferguson CM, Schwarz EM, Reynolds PR, Puzas JE, Rosier RN, O'Keefe RJ

Endocrinology.. 2000 December 141 (12):4728-35. Epub 1900 01 01.

Quantitative small-animal surrogate to evaluate drug efficacy in preventing wear debris-induced osteolysis.

Schwarz EM, Benz EB, Lu AP, Goater JJ, Mollano AV, Rosier RN, Puzas JE, Okeefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2000 November 18 (6):849-55. Epub 1900 01 01.

Targeted deletion of Minpp1 provides new insight into the activity of multiple inositol polyphosphate phosphatase in vivo.

Chi H, Yang X, Kingsley PD, O'Keefe RJ, Puzas JE, Rosier RN, Shears SB, Reynolds PR

Molecular and cellular biology.. 2000 September 20 (17):6496-507. Epub 1900 01 01.

Cloning of the chick BMP1/Tolloid cDNA and expression in skeletal tissues.

Reynolds SD, Zhang D, Puzas JE, O'Keefe RJ, Rosier RN, Reynolds PR

Gene.. 2000 May 2248 (1-2):233-43. Epub 1900 01 01.

PTHrP expression in chondrocytes, regulation by TGF-beta, and interactions between epiphyseal and growth plate chondrocytes.

Pateder DB, Rosier RN, Schwarz EM, Reynolds PR, Puzas JE, D'Souza M, O'Keefe RJ

Experimental cell research.. 2000 May 1256 (2):555-62. Epub 1900 01 01.

Tumor necrosis factor-alpha/nuclear transcription factor-kappaB signaling in periprosthetic osteolysis.

Schwarz EM, Lu AP, Goater JJ, Benz EB, Kollias G, Rosier RN, Puzas JE, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2000 May 18 (3):472-80. Epub 1900 01 01.

BMP-6 is an autocrine stimulator of chondrocyte differentiation.

Grimsrud CD, Romano PR, D'Souza M, Puzas JE, Reynolds PR, Rosier RN, O'Keefe RJ

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 1999 April 14 (4):475-82. Epub 1900 01 01.

Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19.

Chi H, Tiller GE, Dasouki MJ, Romano PR, Wang J, O'keefe RJ, Puzas JE, Rosier RN, Reynolds PR

Genomics.. 1999 March 1556 (3):324-36. Epub 1900 01 01.

Differential activation by cytokines of mitogen-activated protein kinases in bovine temporomandibular-joint disc cells.

Landesberg R, Takeuchi E, Puzas JE

Archives of oral biology.. 1999 January 44 (1):41-8. Epub 1900 01 01.

Interleukin-10 inhibits cytokine synthesis in monocytes stimulated by titanium particles: evidence of an anti-inflammatory regulatory pathway.

Pollice PF, Hsu J, Hicks DG, Bukata S, Rosier RN, Reynolds PR, Puzas JE, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1998 November 16 (6):697-704. Epub 1900 01 01.

Stimulation of bone formation in vivo by insulin-like growth factor-II in rats.

Ishibe M, Ishibashi T, Kaneda K, Koda T, Rosier RN, Puzas JE

Calcified tissue international.. 1998 July 63 (1):36-8. Epub 1900 01 01.

HiPER1, a phosphatase of the endoplasmic reticulum with a role in chondrocyte maturation.

Romano PR, Wang J, O'Keefe RJ, Puzas JE, Rosier RN, Reynolds PR

Journal of cell science.. 1998 March 111 ( Pt 6) :803-13. Epub 1900 01 01.

Bone morphogenetic protein-7 in growth-plate chondrocytes: regulation by retinoic acid is dependent on the stage of chondrocyte maturation.

Grimsrud CD, Rosier RN, Puzas JE, Reynolds PR, Reynolds SD, Hicks DG, O'Keefe RJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1998 March 16 (2):247-55. Epub 1900 01 01.

Modulation of the production of cytokines in titanium-stimulated human peripheral blood monocytes by pharmacological agents. The role of cAMP-mediated signaling mechanisms.

Blaine TA, Pollice PF, Rosier RN, Reynolds PR, Puzas JE, O'Keefe RJ

The Journal of bone and joint surgery. American volume.. 1997 October 79 (10):1519-28. Epub 1900 01 01.

P-glycoprotein expression in cartilaginous tumors.

Rosier RN, O'Keefe RJ, Teot LA, Fox EJ, Nester TA, Puzas JE, Reynolds PR, Hicks DG

Journal of surgical oncology.. 1997 June 65 (2):95-105. Epub 1900 01 01.

Characterization of voltage-sensitive calcium channels in growth plate chondrocytes.

Zuscik MJ, Gunter TE, Puzas JE, Rosier RN

Biochemical and biophysical research communications.. 1997 May 19234 (2):432-8. Epub 1900 01 01.

Osteoclasts constitutively express regulators of bone resorption: an immunohistochemical and in situ hybridization study.

O'Keefe RJ, Teot LA, Singh D, Puzas JE, Rosier RN, Hicks DG

Laboratory investigation; a journal of technical methods and pathology.. 1997 April 76 (4):457-65. Epub 1900 01 01.

Differential regulation of type-II and type-X collagen synthesis by parathyroid hormone-related protein in chick growth-plate chondrocytes.

O'Keefe RJ, Loveys LS, Hicks DG, Reynolds PR, Crabb ID, Puzas JE, Rosier RN

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1997 March 15 (2):162-74. Epub 1900 01 01.

The reversal line may be a key modulator of osteoblast function: observations from an alveolar bone wound-healing model.

Romano PR, Caton JG, Puzas JE

Journal of periodontal research.. 1997 January 32 (1 Pt 2):143-7. Epub 1900 01 01.

Vertebral body osteopenia associated with posterolateral spine fusion in humans.

Myers MA, Casciani T, Whitbeck MG, Puzas JE

Spine.. 1996 October 1521 (20):2368-71. Epub 1900 01 01.

Effects of lead on growth plate chondrocyte phenotype.

Hicks DG, O'Keefe RJ, Reynolds KJ, Cory-Slechta DA, Puzas JE, Judkins A, Rosier RN

Toxicology and applied pharmacology.. 1996 September 140 (1):164-72. Epub 1900 01 01.

Increased levels of tumor necrosis factor-alpha and interleukin-6 protein and messenger RNA in human peripheral blood monocytes due to titanium particles.

Blaine TA, Rosier RN, Puzas JE, Looney RJ, Reynolds PR, Reynolds SD, O'Keefe RJ

The Journal of bone and joint surgery. American volume.. 1996 August 78 (8):1181-92. Epub 1900 01 01.

Sporadic corticosteroid pulses and osteoporosis in multiple sclerosis.

Schwid SR, Goodman AD, Puzas JE, McDermott MP, Mattson DH

Archives of neurology.. 1996 August 53 (8):753-7. Epub 1900 01 01.

Identification and characterization of a unique chondrocyte gene involved in transition to hypertrophy.

Reynolds SD, Johnston C, Leboy PS, O'Keefe RJ, Puzas JE, Rosier RN, Reynolds PR

Experimental cell research.. 1996 July 10226 (1):197-207. Epub 1900 01 01.

Alternative splicing of a chondrocyte-specific gene.

Reynolds PR, Reynolds SD, O'Keefe RJ, Puzas JE, Rosier RN

Annals of the New York Academy of Sciences.. 1996 June 8785 :321-4. Epub 1900 01 01.

Granular histiocytosis of pelvic lymph nodes following total hip arthroplasty. The presence of wear debris, cytokine production, and immunologically activated macrophages.

Hicks DG, Judkins AR, Sickel JZ, Rosier RN, Puzas JE, O'Keefe RJ

The Journal of bone and joint surgery. American volume.. 1996 April 78 (4):482-96. Epub 1900 01 01.

Cellular, biochemical and molecular characterization of the bovine temporomandibular joint disc.

Landesberg R, Takeuchi E, Puzas JE

Archives of oral biology.. 1996 41 (8-9):761-7. Epub 1900 01 01.

17 beta-estradiol increases the receptor number and modulates the action of 1,25-dihydroxyvitamin D3 in human osteosarcoma-derived osteoblast-like cells.

Ishibe M, Nojima T, Ishibashi T, Koda T, Kaneda K, Rosier RN, Puzas JE

Calcified tissue international.. 1995 December 57 (6):430-5. Epub 1900 01 01.

Identification of functional insulin-like growth factor-II/mannose-6-phosphate receptors in isolated bone cells.

Martinez DA, Zuscik MJ, Ishibe M, Rosier RN, Romano PR, Cushing JE, Puzas JE

Journal of cellular biochemistry.. 1995 October 59 (2):246-57. Epub 1900 01 01.

Comparison of the type-2 insulin-like growth factor receptor in normal osteoblasts and osteosarcoma-derived osteoblast-like cells.

Ishibe M, Nojima T, Ishibashi T, Koda T, Rosier RN, Puzas JE, Kaneda K

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1995 September 13 (5):643-8. Epub 1900 01 01.

Shared phenotypic expression of osteoblasts and chondrocytes in fracture callus.

Hughes SS, Hicks DG, O'Keefe RJ, Hurwitz SR, Crabb ID, Krasinskas AM, Loveys L, Puzas JE, Rosier RN

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 1995 April 10 (4):533-44. Epub 1900 01 01.

The mandibular condylar growth center: separation and characterization of the cellular elements.

Landesberg R, Proctor RL, Rosier RN, Puzas JE

Calcified tissue international.. 1995 January 56 (1):71-7. Epub 1900 01 01.

Multiple drug resistance in osteosarcoma.

Rosier RN, Teot LA, Hicks DG, Schwartz C, O'Keefe RJ, Puzas JE

The Iowa orthopaedic journal.. 1995 15 :66-73. Epub 1900 01 01.

Cyclic-AMP-dependent protein kinase activity is not required by parathyroid hormone to stimulate phosphoinositide signaling in chondrocytes but is required to transduce the hormone's proliferative effect.

Zuscik MJ, Puzas JE, Rosier RN, Gunter KK, Gunter TE

Archives of biochemistry and biophysics.. 1994 December 315 (2):352-61. Epub 1900 01 01.

Analysis of type II and type X collagen synthesis in cultured growth plate chondrocytes by in situ hybridization: rapid induction of type X collagen in culture.

O'Keefe RJ, Puzas JE, Loveys L, Hicks DG, Rosier RN

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 1994 November 9 (11):1713-22. Epub 1900 01 01.

Activation of phosphoinositide metabolism by parathyroid hormone in growth plate chondrocytes.

Zuscik MJ, Gunter TE, Rosier RN, Gunter KK, Puzas JE

Cell calcium.. 1994 August 16 (2):112-22. Epub 1900 01 01.

Comparative effects of growth factors on fibroblasts of Dupuytren's tissue and normal palmar fascia.

Alioto RJ, Rosier RN, Burton RI, Puzas JE

The Journal of hand surgery.. 1994 May 19 (3):442-52. Epub 1900 01 01.

Effects of transforming growth factor-beta 1 and fibroblast growth factor on DNA synthesis in growth plate chondrocytes are enhanced by insulin-like growth factor-I.

O'Keefe RJ, Crabb ID, Puzas JE, Rosier RN

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1994 May 12 (3):299-310. Epub 1900 01 01.

Regulation of osteoclastic activity in infection.

Puzas JE, Hicks DG, Reynolds SD, O'Keefe RJ

Methods in enzymology.. 1994 236 :47-58. Epub 1900 01 01.

Low content of estrogen receptors in human giant cell tumors of bone.

Ishibe M, Ishibe Y, Ishibashi T, Nojima T, Rosier RN, Puzas JE, Kaneda K

Archives of orthopaedic and trauma surgery.. 1994 113 (2):106-9. Epub 1900 01 01.

Effects of parathyroid hormone-related peptide on chick growth plate chondrocytes.

Loveys LS, Gelb D, Hurwitz SR, Puzas JE, Rosier RN

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 1993 November 11 (6):884-91. Epub 1900 01 01.

Preliminary findings on the effects of sex hormones on bone metabolism in anorexia nervosa.

Kreipe RE, Hicks DG, Rosier RN, Puzas JE

The Journal of adolescent health : official publication of the Society for Adolescent Medicine.. 1993 June 14 (4):319-24. Epub 1900 01 01.

Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues.

Crabb ID, Hughes SS, Hicks DG, Puzas JE, Tsao GJ, Rosier RN

The American journal of pathology.. 1992 September 141 (3):579-89. Epub 1900 01 01.

Influence of prostaglandins on DNA and matrix synthesis in growth plate chondrocytes.

O'Keefe RJ, Crabb ID, Puzas JE, Rosier RN

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 1992 April 7 (4):397-404. Epub 1900 01 01.

Differential effects of parathyroid hormone on chick growth plate and articular chondrocytes.

Crabb ID, O'Keefe RJ, Puzas JE, Rosier RN

Calcified tissue international.. 1992 January 50 (1):61-6. Epub 1900 01 01.

Osteoblasts and chondrocytes are important target cells for the toxic effects of lead.

Puzas JE, Sickel MJ, Felter ME

Neurotoxicology.. 1992 13 (4):783-8. Epub 1900 01 01.

Human prostatic acid phosphatase directly stimulates collagen synthesis and alkaline phosphatase content of isolated bone cells.

Ishibe M, Rosier RN, Puzas JE

The Journal of clinical endocrinology and metabolism.. 1991 October 73 (4):785-92. Epub 1900 01 01.

Heterotopic ossification: clinical and cellular aspects.

Sawyer JR, Myers MA, Rosier RN, Puzas JE

Calcified tissue international.. 1991 September 49 (3):208-15. Epub 1900 01 01.

Synergistic effect of transforming growth factor beta and fibroblast growth factor on DNA synthesis in chick growth plate chondrocytes.

Crabb ID, O'Keefe RJ, Puzas JE, Rosier RN

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.. 1990 November 5 (11):1105-12. Epub 1900 01 01.

The production of transforming growth factor-beta by chick growth plate chondrocytes in short term monolayer culture.

Gelb DE, Rosier RN, Puzas JE

Endocrinology.. 1990 October 127 (4):1941-7. Epub 1900 01 01.

Cytosolic free calcium concentrations in avian growth plate chondrocytes.

Gunter TE, Zuscik MJ, Puzas JE, Gunter KK, Rosier RN

Cell calcium.. 1990 August 11 (7):445-57. Epub 1900 01 01.

Animal model for disk displacement.

Tallents RH, Macher DJ, Rivoli P, Puzas JE, Scapino RP, Katzberg RW

Journal of craniomandibular disorders : facial & oral

Heterotopic ossification following uncemented total hip arthroplasty.

Rockwood PR, Horne JG

The Journal of arthroplasty.. 1990 5 Suppl :S43-6. Epub 1900 01 01.

Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes.

O'Keefe RJ, Rosier RN, Puzas JE

Connective tissue research.. 1990 24 (1):53-66. Epub 1900 01 01.

Pathologic bone formation.

Puzas JE, Miller MD, Rosier RN

Clinical orthopaedics and related research.. 1989 August (245):269-81. Epub 1900 01 01.

Countercurrent centrifugal elutriation. High-resolution method for the separation of growth-plate chondrocytes.

O'Keefe RJ, Crabb ID, Puzas JE, Rosier RN

The Journal of bone and joint surgery. American volume.. 1989 April 71 (4):607-20. Epub 1900 01 01.

Transforming growth factor beta: an autocrine regulator of chondrocytes.

Rosier RN, O'Keefe RJ, Crabb ID, Puzas JE

Connective tissue research.. 1989 20 (1-4):295-301. Epub 1900 01 01.

Effect of transforming growth factor-beta on DNA synthesis by growth plate chondrocytes: modulation by factors present in serum.

O'Keefe RJ, Puzas JE, Brand JS, Rosier RN

Calcified tissue international.. 1988 December 43 (6):352-8. Epub 1900 01 01.

Effects of transforming growth factor-beta on matrix synthesis by chick growth plate chondrocytes.

O'Keefe RJ, Puzas JE, Brand JS, Rosier RN

Endocrinology.. 1988 June 122 (6):2953-61. Epub 1900 01 01.

Synthesis of 1,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol by calvarial cells. Characterization of the enzyme systems.

Puzas JE, Turner RT, Howard GA, Brand JS, Baylink DJ

The Biochemical journal.. 1987 July 15245 (2):333-8. Epub 1900 01 01.

The stimulus for bone formation.

Puzas JE, Evarts CM, Brand JS

The Hip. 1987 :25-38. Epub 1900 01 01.

Prevention of heterotopic bone formation in high-risk patients by postoperative irradiation.

Evarts CM, Ayers DC, Puzas JE

The Hip. 1987 :70-83. Epub 1900 01 01.

Isolation and characterization of bone cells from a patient with osteogenesis imperfecta type 1b.

Puzas JE, Brand JS, Jackman KV

Bone and mineral. 1986 October 1 (5):373-82. Epub 1900 01 01.

The effect of bone cell stimulatory factors can be measured with thymidine incorporation only under specific conditions.

Puzas JE, Brand JS

Calcified tissue international.. 1986 August 39 (2):104-8. Epub 1900 01 01.

In vitro use of vitamin D3 metabolites: culture conditions determine cell uptake.

Puzas JE, Brand JS

Calcified tissue international.. 1985 September 37 (5):474-7. Epub 1900 01 01.

Bone cell phosphotyrosine phosphatase: characterization and regulation by calcitropic hormones.

Puzas JE, Brand JS

Endocrinology.. 1985 June 116 (6):2463-8. Epub 1900 01 01.

Calvarial cells synthesize 1 alpha,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3.

Turner RT, Howard GA, Puzas JE, Baylink DJ, Knapp DR

Biochemistry.. 1983 March 122 (5):1073-6. Epub 1900 01 01.

Bone cells on microcarrier spheres.

Howard GA, Turner RT, Puzas JE, Nichols F, Baylink DJ

JAMA.. 1983 January 14249 (2):258-9. Epub 1900 01 01.

Cells isolated from embryonic intestine synthesize 1,25-dihydroxyvitamin-D3 and 24,25-dihydroxyvitamin-D3 in culture.

Puzas JE, Turner RT, Howard GA, Baylink DJ

Endocrinology.. 1983 January 112 (1):378-80. Epub 1900 01 01.

Histomorphometry and autoradiography of cultured fetal rat long bones.

Nefussi JR, Vignery A, Puzas JE, Baron R

The Anatomical record.. 1982 October 204 (2):105-12. Epub 1900 01 01.

Electrophoretically separated bone cell types from the foetal rat calvarium: a histochemical and biochemical study.

Puzas JE, Jensen JA

The Histochemical journal.. 1982 July 14 (4):561-71. Epub 1900 01 01.

Adding selected areas of color to black and white slides by color coupling.

Puzas JE, Wilson DE, Treat DE, Brand JS

Journal of biological photography. 1982 July 50 (3):69-70. Epub 1900 01 01.

Effect of skeletal alkaline phosphatase inhibitors on bone cell proliferation in vitro.

Farley JR, Puzas JE, Baylink DJ

Mineral and electrolyte metabolism.. 1982 June 7 (6):316-23. Epub 1900 01 01.

Coupling factor.

Baylink D, Farley J, Howard G, Drivdahl R, Puzas E, Masuda T, Ivey J, Gruber H

Advances in experimental medicine and biology.. 1982 151 :409-21. Epub 1900 01 01.

Regulation of DNA synthesis in chick calvaria cells by factors from bone organ culture.

Drivdahl RH, Puzas JE, Howard GA, Baylink DJ

Proceedings of the Society for Experimental Biology and Medicine.. 1981 November 168 (2):143-50. Epub 1900 01 01.

Endogenous inhibitor of bone cell proliferation.

Puzas JE, Drivdahl RH, Howard GA, Baylink DJ

Proceedings of the Society for Experimental Biology and Medicine.. 1981 January 166 (1):113-22. Epub 1900 01 01.

In vitro synthesis of 1 alpha,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol by isolated calvarial cells.

Turner RT, Puzas JE, Forte MD, Lester GE, Gray TK, Howard GA, Baylink DJ

Proceedings of the National Academy of Sciences of the United States of America.. 1980 October 77 (10):5720-4. Epub 1900 01 01.

Metabolism of 25(OH)D3 to 1,25(OH)2D3 and 24,25(OH)2D3 by chick chorioallantoic cells in culture.

Puzas JE, Turner RT, Forte MD, Kenny AD, Baylink DJ

General and comparative endocrinology.. 1980 September 42 (1):116-22. Epub 1900 01 01.

Isolation of specific bone cell types by free-flow electrophoresis.

Puzas JE, Vignery A, Rasmussen H

Calcified tissue international.. 1979 July 327 (3):263-8. Epub 1900 01 01.

Parathyroid hormone stimulation of collagenase secretion by isolated bone cells.

Puzas JE, Brand JS

Endocrinology.. 1979 February 104 (2):559-62. Epub 1900 01 01.

A rapid assay for cellular deoxyribonucleic acid.

Puzas JE, Goodman DB

Analytical biochemistry.. 1978 May 86 (1):50-5. Epub 1900 01 01.

Collagenolytic activity from isolated bone cells.

Puzas JE, Brand JS

Biochimica et biophysica acta.. 1976 May 13429 (3):964-74. Epub 1900 01 01.