Karen L. Bentley, M.S.

Karen L. Bentley, M.S.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 626
Rochester, NY 14642

Office: (585) 275-1954

Lab Information

The purpose of the URMC Electron Microscope Research Core Facility is to provide up-to-date and cost-effective support to researchers whose studies involve the use of electron microscopy.

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Professional Bio

Ms.(de Mesy) Bentley has 29 years of experience in Ultrastructural Pathology and is considered an expert in both diagnostic and research electron microscopy. She has also been responsible for the development of numerous Diagnostic EM teaching sets for Pathology residents and other departmental resident trainees. Ms. Bentley has been innovative in the development of EM customized techniques to support both EM based clinical and research projects. At present, she is the Director of the Medical Center's Electron Microscope Shared Resource Facility.

Research Bio

Ms. (de Mesy) Bentley is currently, the Director of the Electron Microscopy Shared Resource and has 29 years of teaching and diagnostic experience in Ultrastructural Pathology, Immunoelectron Microscopy, Hematology, Dental Research, Infectious Agents, Biofilm Formation, Nanoparticle Characterization, Morphometric Analysis and X-ray Dispersive Spectroscopy using both Transmission and Scanning Electron Microsocopes.

In 2011, Ms. Bentley was recruited by Dr. Edward Schwarz, the Director of the Center for Musculoskeletal Research in Orthopaedics, to investigate and analyze a mouse model of Staphylococcus aureus (MRSA and MSSA strains) infected implants and biofilm formation using scanning electron microscopy (SEM) which resulted in two Orthopaedic Research Society journal covers.

In 2014, she initiated transmission electron microscopy (TEM) studies on the infected bone which had surrounded the biofilm laden implants. Ms. Bentley's high magnification TEM study led to the discovery of S. aureus' deforming into submicron rod-shaped bacteria to colonize the ostetocytic-lacunar canaliculi network of bone. This was the first evidence of S. aureus, a round non-motile 1-1.5 micron sized bacteria, becoming rod-shaped to enter (using asymmetric binary fission) the narrow confines of (0.4-0.2 micron diameter) canaliculi within live bone. This finding was published in the Journal of Bone and Mineral Research in 2016 and was also chosen for a book cover, Osteoimmunology, 2nd Ed, 2016.

She has also authored and co-authored over 50 research publications resulting from her years of collaborative TEM and SEM imaging support to researchers here at the University. URMC Researchers who require high magnification imaging support can request a consultation and an estimate of cost prior to starting a research project. This facility provides full wet-lab services for processing, sectioning and utilizes a Hitachi 7650 TEM/STEM electron microscope with an attached 11 megapixel Gatan digital camera system. It is located in the basement of the Medical School building.

Awards & Honors (Local)

Current Concepts in Ultrastructural Pathology | Ultrapath IV | Ullensvang, Norway 1988
M.S. Anatomy/Pathology 1984
B.S. Cum laude | University of Rochester 1982

Recent Journal Articles

Showing the 5 most recent journal articles. 58 available »

2016 Aug 8
Carli AV, Warth LC, de Mesy Bentley KL, Nestor BJ. "Short to Midterm Follow-Up of the Tritanium Primary Acetabular Component: A Cause for Concern." The Journal of arthroplasty. 2016 Aug 8; Epub 2016 Aug 08.
2016 Jan 15
Shum LC, White NS, Mills BN, de Mesy Bentley KL, Eliseev RA. "Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation." Stem cells and development. 2016 Jan 15; 25(2):114-22. Epub 2015 Dec 10.
2016
de Mesy Bentley KL; Trombetta R; Nishitani K; Bello-Irizarry SN; Ninomiya M; Zhang L; Chung HL; McGrath JL; Daiss JL; Awad HA; Kates SL; Schwarz EM. "Evidence of Staphylococcus aureus Deformation, Proliferation and Migration in Canaliculi of Live Cortical Bone in Murine Models of Osteomyelitis." Journal of Bone and Mineral Research, Dec 8. doi: 10.1002/jbmr.3055. [Epub ahead of print]. 2016; .
2015 Sep
Nishitani K, Sutipornpalangkul W, de Mesy Bentley KL, Varrone JJ, Bello-Irizarry SN, Ito H, Matsuda S, Kates SL, Daiss JL, Schwarz EM. "Quantifying the natural history of biofilm formation in vivo during the establishment of chronic implant-associated Staphylococcus aureus osteomyelitis in mice to identify critical pathogen and host factors." Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2015 Sep; 33(9):1311-9. Epub 2015 May 18.
2015 Jan 1
Trembley MA, Velasquez LS, de Mesy Bentley KL, Small EM. "Myocardin-related transcription factors control the motility of epicardium-derived cells and the maturation of coronary vessels." Development. 2015 Jan 1; 142(1):21-30.

Current Appointments

Associate - Department of Pathology and Laboratory Medicine (SMD) - Primary
Associate - Center for Musculoskeletal Research

Education

MS | Anatomy | Univ Rochester Sch Med/Dent1984
BS | Biology | University of Rochester1982