CONTACT INFORMATIONCREDENTIALSAWARDSPUBLICATIONSAlan E. Friedman, Ph.D.Contact InformationPhone NumbersOffice: (585) 273-4066LocationsUniversity of Rochester Medical CenterSchool of Medicine and Dentistry601 Elmwood Ave, Box EHSCRochester, NY 14642BiographyResearchThe evaluation of biomarkers as diagnostic indicators for disease progression is an endeavor of intense academic and clinical research. Our group is interested in the regulation of cancer progression, which we are studying through a novel in vitro cell model system. This model, called the Prostatic Human Epithelial Cancer (PHEC) system, represents all three states of human prostate cancer progression: non-tumorigenic, tumorigenic and metastatic cells. For each state, we have individual cell lines in culture, and thus we are able to compare the three states directly, as they are generated from the same progenitor line. This allows us to study changes in expressed protein identities, concentrations, biomolecular interaction partners, and even genetic expression profiles, to understand the sub-cellular basis for prostate cancer progression. Our research focuses on the identification of aberrant proteins and subtle changes in concentration in response to cellular changes, using mass spectrometry and other cutting edge protein chemistry tools. With these tools, we recently defined the behavior of one such protein, nucleoside diphosphate kinase A, or non-metastatic 23 H1 form (NM23-H1). Doing a comparative analysis of this protein in our PHEC cell lines, we discovered that NM23-H1 has a consistent regression in tumorigenic and metastatic states of cancer compared to its levels in the non-tumorigenic state, as illustrated by immunochemistry in Figure 1. This protein is the only known human histidine kinase, and may serve as the entry point for a two-component signal transduction (2CST), a style of protein regulation that is common in prokaryotes but seldom seen in eukaryotes above yeast. 2CST systems rely upon a histidine kinase for autophosphorylation, followed by the transfer of the phosphate group to a response regulator (RR), usually on an aspartic acid residue. The proposal of the role of NM23-H1 in such a 2CST system is outlined in Scheme 1. This is a new paradigm for the regulation of proteins in humans, and our laboratory is working to provide further evidence for the existence of the 2CST system in humans and to characterize its role in the progression of prostate cancer and its metastasis outside the prostate.CredentialsEducation1982BS | San Diego State UniversityChemistry1988PhD | University of California, Santa BarbaraChemistryAwards2012Research Funding Undergraduate Research (Michael Nevid)Location: University of Rochester2010Provost's Multidisciplinary AwardSponsor: University of Rochester1998 - 2000European Consortium on PeroxidasesSponsor: Johnson and Johnson Clinical Diagnostics1993Distinguished Service AwardSponsor: Eastman Kodak CompanyLocation: Rochester, NY1991Distinguished Service AwardSponsor: Eastman Kodak CompanyLocation: Rochester, NY1990 - 1991Research FellowshipSponsor: National Institute of Health/National Cancer InstituteLocation: California Institute of Technology, Pasadena, CA1988 - 1989Postdoctoral Research FellowshipSponsor: National institute of Health/National Cancer InstituteLocation: Columbia University, New York, NY1986Chancellors Patent Fund GrantSponsor: University of California at Santa Barbara1980American Chemical Society Summer FellowshipSponsor: San Diego State University, CAUniversity Regents FellowshipSponsor: University of California at Santa Barbara, CALocation: 1982, 1984, 1986VIEW ALL expand_morePublicationsJournal Articles10/30/2015Polevoda B, McDougall WM, Tun BN, Cheung M, Salter JD, Friedman AE, Smith HC. "RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates." Nucleic acids research.. 2015 Oct 30; 43(19):9434-45. Epub 2015 Sep 30. 2/2015Lapek JD, Tombline G, Kellersberger KA, Friedman MR, Friedman AE. "Evidence of histidine and aspartic acid phosphorylation in human prostate cancer cells." Naunyn-Schmiedeberg's archives of pharmacology.. 2015 Feb 0; 388(2):161-73. Epub 2014 Nov 06. 2015Li Q, Kappil MA, Li A, Dassanayake PS, Darrah TH, Friedman AE, Friedman M, Lambertini L, Landrigan P, Stodgell CJ, Xia Y, Nanes JA, Aagaard KM, Schadt EE, Murray JC, Clark EB, Dole N, Culhane J, Swanson J, Varner M, Moye J, Kasten C, Miller RK, Chen J. "Exploring the associations between microRNA expression profiles and environmental pollutants in human placenta from the National Children's Study (NCS)." Epigenetics.. 2015 10(9):793-802. Epub 2015 Aug 07. VIEW ALL PUBLICATIONSClose WindowSchedule an appointment with Alan E. Friedman, Ph.D.Please answer the following questions to help us find the right appointment for you.Important: If you believe that you have a medical or psychiatric emergency, please call 911 or go to the nearest hospital. This website is not intended for emergency care.Have you seen this provider in the last 2 years?YesNoExisting Patient Schedule or request a follow up appointment online through MyChart. If you do not have a MyChart account, please close this window and call the appointment phone number. Alan E. Friedman, Ph.D. is currently scheduling for the following appointment type(s): Our policy does not permit patients to establish care with multiple providers within the same practice or specialty without prior approval. If you choose a new provider in the same office, we will cancel the appointment. Please contact the office directly with questions on this policy.