- 1994: MSc Degree in Biology awarded by the University of Navarra (Spain).
- 1996-2000: PhD Thesis. Estudio de las interacciones entre las proteínas implicadas en los procesos de replicación y transcripción del Virus Respiratorio Sincitial. Mentor: José Antonio Melero Fondevila. Research Center: Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III. Madrid, Spain.
- 2000-2007: Post-doctoral fellow. Mentor: Dr. Adolfo García-Sastre. Research Center: Mount Sinai School of Medicine. Microbiology Department. 1 Gustave L. Levy Place, New York, NY, 10029.
- 2007 (January)-2008 (July): Research Assistant Professor.
Research Center: Mount Sinai School of Medicine. Microbiology Department. 1 Gustave L. Levy Place, New York, NY, 10029.
- 2008 (August)-present: Assistant Professor. Research Center: University of Rochester School of Medicine and Dentistry. Department of Microbiology and Immunology. 601 Elmwood Avenue, Box 672, Rochester, NY, 14642.
- 2012 (January)-present: External Professor Universidad de Merida en Yucatan, Mexico.
My research interest for the last decade has been focused on the molecular biology, immunology, and pathogenesis of negative-strand (Influenza viruses, Respiratory Syncytial Virus, Human Metapneumovirus, arenavirus, Thogoto virus, Ebola virus, Crimean Congo Hemorrhagic Fever virus) and positive-strand (Dengue virus, SARS coronavirus, Mouse Hepatitis virus) RNA, and DNA viruses. I have extensive knowledge in reverse genetics techniques for rescuing RNA and DNA viruses, pioneered the development of techniques and screening assays to identify viral-encoded interferon antagonist proteins, and established new molecular biology techniques to study highly pathogenic viruses without the requirement of special biosafety conditions.
My major contributions to the virology field include the development of new experimental systems to answer difficult questions in the mechanisms of virus-host interactions, including: 1) Reverse genetics tools to genetically manipulate viruses, that I have used to examine virus-host interactions and development of vaccines; 2) Plasmid, virus and cellular based assays for the identification of virus-encoded IFN-antagonist proteins that I have used to uncover molecular mechanisms involved in viral pathogenesis, including the first description of an IFN antagonist protein in the arenavirus family and the multiple anti-IFN actions of influenza virus NS1; and 3) Single-cycle infectious influenza and arenaviruses to study highly pathogenic viruses, without the requirement of special biosafety conditions.
Since my recent appointment in the Department of Microbiology and Immunology at University of Rochester, one of the 5 NIAID Centers of Excellence for Influenza Research and Surveillance, my research has focused on the interactions of influenza and arenavirus proteins with the host cell. My experience in molecular biology, virus reverse genetics techniques, and virus-based bioassays to study the interferon response gives me the unique opportunity to carry out innovative and original research projects, the major objective of which is to shed light and understand the molecular determinants of patogenesis of influenza and arenaviruses and how these viruses interact with their hosts.
Nogales A, Huang K, Chauché C, DeDiego ML, Murcia PR, Parrish CR, Martínez-Sobrido L. "Canine influenza viruses with modified NS1 proteins for the development of live-attenuated vaccines." Virology. 2017 Jan; 500:1-10. Epub 2016 Oct 15.
2016 Nov 1
DeDiego ML, Nogales A, Lambert-Emo K, Martinez-Sobrido L, Topham DJ. "NS1 Protein Mutation I64T Affects Interferon Responses and Virulence of Circulating H3N2 Human Influenza A Viruses." Journal of virology. 2016 Nov 1; 90(21):9693-9711. Epub 2016 Oct 14.
2016 Oct 2
Romero-Beltran L, Baker SF, Puerto-Solís M, González-Losa R, Conde-Ferraez L, Alvarez-Sánchez LC, Martínez-Sobrido L, Ayora-Talavera G. "Mutations at highly conserved residues in influenza A(H1N1)pdm09 virus affect neuraminidase activity." Virus research. 2016 Oct 2; 225:1-9. Epub 2016 Sep 03.
Lenartowicz E, Nogales A, Kierzek E, Kierzek R, Martínez-Sobrido L, Turner DH. "Antisense Oligonucleotides Targeting Influenza A Segment 8 Genomic RNA Inhibit Viral Replication." Nucleic acid therapeutics. 2016 Oct; 26(5):277-285. Epub 2016 Jul 27.
Martinez-Sobrido L, de la Torre JC. "Novel strategies for development of hemorrhagic fever arenavirus live-attenuated vaccines." Expert review of vaccines. 2016 Sep; 15(9):1113-21. Epub 2016 May 13.