- 1994: MSc Degree in Biology awarded by the University of Navarra (Spain).
- 1996-2000: PhD Thesis. Estudio de las interacciones entre las proteínas implicadas en los procesos de replicación y transcripción del Virus Respiratorio Sincitial. Mentor: José Antonio Melero Fondevila. Research Center: Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III. Madrid, Spain.
- 2000-2007: Post-doctoral fellow. Mentor: Dr. Adolfo García-Sastre. Research Center: Mount Sinai School of Medicine. Microbiology Department. 1 Gustave L. Levy Place, New York, NY, 10029.
- 2007 (January)-2008 (July): Research Assistant Professor.
Research Center: Mount Sinai School of Medicine. Microbiology Department. 1 Gustave L. Levy Place, New York, NY, 10029.
- 2008 (August)-present: Assistant Professor. Research Center: University of Rochester School of Medicine and Dentistry. Department of Microbiology and Immunology. 601 Elmwood Avenue, Box 672, Rochester, NY, 14642.
- 2012 (January)-present: External Professor Universidad de Merida en Yucatan, Mexico.
My research interest for the last decade has been focused on the molecular biology, immunology, and pathogenesis of negative-strand (Influenza viruses, Respiratory Syncytial Virus, Human Metapneumovirus, arenavirus, Thogoto virus, Ebola virus, Crimean Congo Hemorrhagic Fever virus) and positive-strand (Dengue virus, SARS coronavirus, Mouse Hepatitis virus) RNA, and DNA viruses. I have extensive knowledge in reverse genetics techniques for rescuing RNA and DNA viruses, pioneered the development of techniques and screening assays to identify viral-encoded interferon antagonist proteins, and established new molecular biology techniques to study highly pathogenic viruses without the requirement of special biosafety conditions.
My major contributions to the virology field include the development of new experimental systems to answer difficult questions in the mechanisms of virus-host interactions, including: 1) Reverse genetics tools to genetically manipulate viruses, that I have used to examine virus-host interactions and development of vaccines; 2) Plasmid, virus and cellular based assays for the identification of virus-encoded IFN-antagonist proteins that I have used to uncover molecular mechanisms involved in viral pathogenesis, including the first description of an IFN antagonist protein in the arenavirus family and the multiple anti-IFN actions of influenza virus NS1; and 3) Single-cycle infectious influenza and arenaviruses to study highly pathogenic viruses, without the requirement of special biosafety conditions.
Since my recent appointment in the Department of Microbiology and Immunology at University of Rochester, one of the 5 NIAID Centers of Excellence for Influenza Research and Surveillance, my research has focused on the interactions of influenza and arenavirus proteins with the host cell. My experience in molecular biology, virus reverse genetics techniques, and virus-based bioassays to study the interferon response gives me the unique opportunity to carry out innovative and original research projects, the major objective of which is to shed light and understand the molecular determinants of patogenesis of influenza and arenaviruses and how these viruses interact with their hosts.
Breen M, Nogales A, Baker SF, Perez DR, Martínez-Sobrido L. "Replication-Competent Influenza A and B Viruses Expressing a Fluorescent Dynamic Timer Protein for In Vitro and In Vivo Studies." PloS one. 2016 11(1):e0147723. Epub 2016 Jan 25.
2015 Nov 15
Nogales A, Rodríguez-Sánchez I, Monte K, Lenschow DJ, Perez DR, Martínez-Sobrido L. "Replication-competent fluorescent-expressing influenza B virus." Virus research. 2015 Nov 15; 213:69-81. Epub 2015 Nov 15.
2015 Oct 15
Halliley JL, Khurana S, Krammer F, Fitzgerald T, Coyle EM, Chung KY, Baker SF, Yang H, Martínez-Sobrido L, Treanor JJ, Subbarao K, Golding H, Topham DJ, Sangster MY. "High-Affinity H7 Head and Stalk Domain-Specific Antibody Responses to an Inactivated Influenza H7N7 Vaccine After Priming With Live Attenuated Influenza Vaccine." The Journal of infectious diseases. 2015 Oct 15; 212(8):1270-8. Epub 2015 Apr 02.
2015 Jul 26
Nogales A, Baker SF, Domm W, Martínez-Sobrido L. "Development and applications of single-cycle infectious influenza A virus (sciIAV)." Virus research. 2015 Jul 26; Epub 2015 Jul 26.
2015 Jul 9
Baker SF, Nogales A, Santiago FW, Topham DJ, Martínez-Sobrido L. "Competitive detection of influenza neutralizing antibodies using a novel bivalent fluorescence-based microneutralization assay (BiFMA)." Vaccine. 2015 Jul 9; 33(30):3562-70. Epub 2015 Jun 01.