Davender Khera was born in Pittsburgh, PA. He completed his undergraduate studies at Yale University in 2001, receiving a BA in economics. He completed medical school at Yale University in 2006. He completed his internship in internal medicine at Massachusetts General Hospital in Boston, MA and his residency in neurology at Massachusetts General and Brigham and Women's Hospital (Partners Healthcare) in Boston, MA in 2010. From 2010-2012, he worked as a general neurologist at Cambridge Hospital (Harvard affiliated) in Cambridge, MA. He joined the University of Rochester in 2012 as a general neurologist.