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Dorota Piekna-Przybylska

TitleResearch Assistant Professor
InstitutionSchool of Medicine and Dentistry
DepartmentMicrobiology and Immunology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 672
Rochester NY 14642
HIV Replication and Latency: The Role of RNA and DNA G-quadruplexes.
G-quadruplexes are guanine rich nucleic acid structures capable of forming a four-stranded structure. DNA G-quadruplexes have been identified in telomeric DNA as well as more recently in mammalian chromosomal DNA - and are considered attractive targets in anti-cancer drug development studies.
We have recently shown that guanine-rich sequences in the HIV-1 genome can adopt G-quadruplex structures in both viral RNA and DNA. Viral RNA G-quadruplexes support genome dimerization and may facilitate viral recombination, which contributes to viral diversity. We have also identified G-quadruplexes in the promoter region of HIV-1 proviral DNA (Sp1 binding sites). This suggests that these structures may also contribute to the regulation of viral gene expression (i.e., regulation of latency and reactivation). Our current research focuses on understanding the role of G-quadruplexes in HIV-1 DNA, and determining whether these structures may represent a new target for antiviral therapies.

 Selected Publications
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  1. Piekna-Przybylska D, Sullivan MA, Sharma G, Bambara RA. U3 Region in the HIV-1 Genome Adopts a G-Quadruplex Structure in Its RNA and DNA Sequence. Biochemistry. 2014 Apr 29; 53(16):2581-93.
    View in: PubMed
  2. Piekna-Przybylska D, Sharma G, Bambara RA. Mechanism of HIV-1 RNA Dimerization in the Central Region of the Genome and Significance for Viral Evolution. J Biol Chem. 2013 Aug 16; 288(33):24140-50.
    View in: PubMed
  3. Piekna-Przybylska D, Bambara RA. Requirements for efficient minus strand strong-stop DNA transfer in human immunodeficiency virus 1. RNA Biol. 2011 Mar-Apr; 8(2):230-6.
    View in: PubMed
  4. Piekna-Przybylska D, Dykes C, Demeter LM, Bambara RA. Sequences in the U3 region of human immunodeficiency virus 1 improve efficiency of minus strand transfer in infected cells. Virology. 2011 Feb 20; 410(2):368-74.
    View in: PubMed
  5. Piekna-Przybylska D, DiChiacchio L, Mathews DH, Bambara RA. A sequence similar to tRNA 3 Lys gene is embedded in HIV-1 U3-R and promotes minus-strand transfer. Nat Struct Mol Biol. 2010 Jan; 17(1):83-9.
    View in: PubMed
  6. Baudin-Baillieu A, Fabret C, Liang XH, Piekna-Przybylska D, Fournier MJ, Rousset JP. Nucleotide modifications in three functionally important regions of the Saccharomyces cerevisiae ribosome affect translation accuracy. Nucleic Acids Res. 2009 Dec; 37(22):7665-77.
    View in: PubMed
  7. Liu B, Liang XH, Piekna-Przybylska D, Liu Q, Fournier MJ. Mis-targeted methylation in rRNA can severely impair ribosome synthesis and activity. RNA Biol. 2008 Oct-Dec; 5(4):249-54.
    View in: PubMed
  8. Piekna-Przybylska D, Przybylski P, Baudin-Baillieu A, Rousset JP, Fournier MJ. Ribosome performance is enhanced by a rich cluster of pseudouridines in the A-site finger region of the large subunit. J Biol Chem. 2008 Sep 19; 283(38):26026-36.
    View in: PubMed
  9. Piekna-Przybylska D, Decatur WA, Fournier MJ. The 3D rRNA modification maps database: with interactive tools for ribosome analysis. Nucleic Acids Res. 2008 Jan; 36(Database issue):D178-83.
    View in: PubMed
  10. Piekna-Przybylska D, Decatur WA, Fournier MJ. New bioinformatic tools for analysis of nucleotide modifications in eukaryotic rRNA. RNA. 2007 Mar; 13(3):305-12.
    View in: PubMed
  11. Piekna-Przybylska D, Liu B, Fournier MJ. The U1 snRNA hairpin II as a RNA affinity tag for selecting snoRNP complexes. Methods Enzymol. 2007; 425:317-53.
    View in: PubMed
  12. Decatur WA, Liang XH, Piekna-Przybylska D, Fournier MJ. Identifying effects of snoRNA-guided modifications on the synthesis and function of the yeast ribosome. Methods Enzymol. 2007; 425:283-316.
    View in: PubMed
  13. Piekna-Przybylska D, Augustyniak H. [Import of protein into mitochondria]. Postepy Biochem. 2002; 48(4):262-74.
    View in: PubMed


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