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Animal - BD LSRII - FACSDiva based

MIFLOWCYT information specific for this instrument can be found in the FCC_Library.

Detailed information about the QC can be found on the baseline report (available upon request).

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

To design a panel using Fluorofinder please click here.

training videos can be accessed here

LSRII Configuration: Low starting voltages as of Jan 2023 posted at the machine and available via FCC_Library.

BLUE 488 LASER (100mW):

Detector

LP

Band Pass

Fluor's (check Fluorofinder for more info)

BLUE A

685

710/50

PerCP-Cy5.5

BLUE B

505

515/20

FITC, CFSE, GFP, Alexa 488

BLUE C

  488/10 SSC

FSC

 

 

 

RED 633 LASER (35mW):

Detector

LP

Band Pass

Fluor's (check Fluorofinder for more info)

RED A

740

780/60

APC-Cy7, APC-Alexa 750

RED B

685 710/50 APC-Cy5.5, Alexa 700, Alexa 680

RED C

 

660/20

APC, Alexa 647

VIOLET 407 LASER (65mW):

Detector

LP

Band Pass

Fluor's (check Fluorofinder for more info)

VIOLET A

740

780/60

Qdot 800, BV 786

VIOLET B

670

705/70

Qdot 705, BV 711

VIOLET C

630

660/40

Qdot 655, BV 650

VIOLET D 595 605/40 Qdot 605, BV 605
VIOLET E 570 585/42 Qdot 585
VIOLET F 557 560/40 Qdot 565
VIOLET G 535 550/40 Pacific Orange, BV 510, Qdot 545
VIOLET H   450/50 Pacific Blue, BV 421,Alexa 405

GREEN 532 LASER (50mW):

Detector

LP

Band Pass

Fluor's (check Fluorofinder for more info)

GREEN A

740

780/40

PE-Cy7, PE-Alexa 750

GREEN B

690

710/50

PE-Cy5.5

GREEN C

640 660/40 7AAD, PE-Cy5

GREEN D

600

610/20

PE-Alexa 610, PE-Texas Red

GREEN E

 

575/25

PE, PI, Alexa 532