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URMC / Research / Flow Cytometry / Services / Instruments / Analytical / Bunsen

Dr. Bunsen Honeydew Configuration

Suggested voltages current as of Dec 22, 2015.

Detailed information about this instrument can be found in the baseline report (available upon request).

FACSCanto II Configuration:

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

 Low Starting Voltage

BLUE A

735

780/60

PE-Cy7

 550

BLUE B

655

670LP

PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP

 550

BLUE C

 610 - - -

BLUE D

556

585/42

PE, PI

400

BLUE E

502

530/30

FITC, GFP, YFP, AlexaFluor 488,

 500

BLUE F

-

488/10

SSC

  
BLUE G - -    

BLUE H

---

-

 

 

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

 Low Starting Voltage

RED A

735

780/60

APC-Cy7

 500

RED B

 685 -    

RED C

---

660/20

APC, Alexa 647, ToPro3

 500

VIOLET 405 LASER (25mW):

Detector

LP

Band Pass

Fluor's

 Low Starting Voltage

VIOLET A

502

550/50

Pacific Orange, LiveDead Orange

 450

VIOLET B

 

450/50

Pacific Blue, BV 421, DAPI, Vibrant Violet

 350

VIOLET C

-

-