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Instruments

Laser Scanning Confocal Microscope I (FV1000 Olympus)

FV1000

Microscope of choice for the following studies:

  • Slides with fluorescent labels on cultured cells or tissue sections which require high resolution imaging
  • Samples up to 100 um thicknesses
  • Live imaging studies both short- and long-term including FRET, FRAP, FLIP, Photobleaching
  • Multiple time point ("mark and find" studies) and studies requiring image tiling
  • All fluorophores within the visible range with excitations beginning at 405nm can be imaged with this spectral laser confocal system. See below for laser lines.

Summary of Instrument Features

  • Microscope: Olympus IX81 (INVERTED microscope configuration i.e. objectives below the sample)
  • Objectives:
    • Normal configuration: 4x (N.A. 0.16),, 10x (N.A. 0.40), 20x oil (N.A. 0.85), 40x oil (N.A. 1.30), 60x oil (N.A. 1.42), 100x oil (N.A. 1.40).
    • Dry objectives available: 20x dry (N.A. 0.75), 40x dry (N.A. 0.60), 60x dry (N.A. 0.70)
    • Water objective available: 60x water
  • DIC Capabilities: 20x, 40x, 60x, 100x
  • Lasers: 405 diode laser, 440 diode laser, 488/515 argon laser, 559 diode laser, 635 diode laser
  • Stage: Prior, Inc. motorized stage, includes tiling and multi-point mark and find memory function
  • Stage Holders: Multiple stage holder formats are provided by the Core. Current stage holders will support: 1 x 3 slides, 2 x 3 slides, circular (all sizes) glass-bottom culture dishes, well plates with glass-coverslip bottoms
  • Environmental Chamber: FULL STAGE INCUBATOR on the microscope, with CO2, temperature, and humidity regulation. Short- or long-term live imaging studies including FRET, FRAP, Multiple point timelapse imaging, etc. are possible
  • New Binder Incubator in room: Cultures can be equilibrated for a number of hours before being transferred into the stage incubator with identical C02, temp. and humidity conditions.
  • Service Contract Support: The FV1000 is under full service contract by Olympus.
  • Opportunities to work directly with Olympus reps: The core organizes multiple opportunities for PIs and staff to talk or work directly with the Olympus rep when they are on-campus.

Conventional Microscope (Olympus VanoxAH-2)

Vanox AH-2

Microscope of choice for the following study types:

  • Studies needing brightfield microscopy

Summary of Instrument Features

  • Microscope: Upright microscope configuration (i.e. objectives above the sample)
  • Objectives: 4x, 10x, 20x, 40x, 100x
  • Lamp source: Halogen lamp
  • Stage: Prior Stage
  • Camera: MicroFire Color Camera
  • Image capture software: PictureFrame

Laser Capture Microdissection Microscope (Zeiss Palmbeam)Zeiss Palmbeam Laser Capture

Microscope of choice for the following study types:

  • Capture of groups of phenotypically similar cells from fresh frozen, fixed then frozen, FFPE tissue onto eppendorf tube caps for further analysis  by  DNA, RNA, and protein assays
  • Capture of cultured cells for further expansion or downstream processing for DNA, RNA, and protein analyses

Summary of Instrument Features

  • Microscope:  Upright microscope configuration (i.e. objectives above sample)
  • Objectives:  10x, 20x, 40x, 63x  dry  - long working distances (able to see through back of slides with good resolution)
  • LED fluorescence lightsource:  Xcite Lamp
  • Capture laser:  Pulsed UV laser (100 nanosecond pulses)
  • Cap Holder:  Holds two caps
  • Sample holders:
    • for 1x 3 slides – holds 3 slides at a time
  • Cameras: two cameras:
    • CC1 color camera
    • mRM camera for fluorescence
      {Note:  Both cameras provide the ability to capture “before cut, after cut, and on-cap” images}
  • Stage:  motorized stage

High Definition Camera with Adjustable Photography Stand for Large Specimen Imaging

Large Specimen High Def Camera

Imaging equipment of choice for:

  • Studies requiring comparison of histologically processed sections to the original thick slice of tissue and original organ (i.e. comparative microscopy for gross organ, macro slices to paraffin-embedded histology samples).

Summary of Instrument Features

  • Stand: Kodak adjustable photography stand
  • Camera: SPOT Camera
  • Software: Image Pro Plus

Image Analysis Capabilities

**NEW WORKSTATION COMING March 2017**

  • Image Pro Plus (v7): Basic morphometry package (cell counting and measuring)
  • FV1000 (Fluoview 4.2): Basic analysis of confocal images. Limited 3d capabilities.
  • FIJI/Image J: NIH standard for image analysis.
  • Amira (though SMDNAS shared licensing). Powerful 3d image processing program.
  • Ability to analyze large data sets through shared computing with UR CIRC and the UR SuperComputer (BlueHive).